BACKGROUND: Chemotaxis is an important step durin the invasion of carcinoma cells. And integrins are most im portant receptors mediating interaction between cells an extracellular matrix ( ECM). This study was designe...BACKGROUND: Chemotaxis is an important step durin the invasion of carcinoma cells. And integrins are most im portant receptors mediating interaction between cells an extracellular matrix ( ECM). This study was designed t study integrin betal mediating chemotaxis of hepatocellula carcinoma (HCC) cells to laminin (LN). METHODS: A micropipette technique was adopted to in vestigate the effect of blockade of integrin betal on pseudo pod protrusion of HCC cells in response to LN stimulation Chemotactic pseudopod protrusion of a HCC cell was eva luated using a dual-pipette set-up, in which two pipette filled with LN solution were positioned in close contact wit the same cell, and pseudopod protrusion into each pipett was viewed dynamically and recorded with a tape recorder The lengths of pseudopods were measured and plotte against time to obtain a pseudopod growth curve. The in tegrin betal subunit on the surfaces of HCC cells were ana lyzed by flow cytometry. RESULTS: In dual pipette chemotaxis experiment, whe the two pipettes were filled with LN(50 μg/ml, 200 μg/ml) pseudopods extended from the HCC cell into each of th pipettes nearly symmetrically, ie, with nearly identica maximum pseudopod length and similar pseudopod growth curves. Upon addition of anti-CD29 (20 μg/ml) to one o the pipettes, pseudopod protrusion was blocked nearly completely while protrusion into the opposite pipette be came more evidently, with a larger maximum length. Ex pression of integrin betal was up to 95.78% to cells chosen in the experiment. CONCLUSION: Integrin betal subunit was an importan constituent receptor subunit for mediating chemotactic pseudopod protrusion of HCC cell to LN.展开更多
Background The mechanical microenvironment of the chondrocytes plays an important role in cartilage homeostasis and in the health of the joint. The pericellular matrix, cellular membrane of the chondrocytes, and their...Background The mechanical microenvironment of the chondrocytes plays an important role in cartilage homeostasis and in the health of the joint. The pericellular matrix, cellular membrane of the chondrocytes, and their cytoskeletal structures are key elements in the mechanical environment. The aims of this study are to measure the viscoelastic properties of isolated chondrons and chondrocytes from rabbit knee cartilage using micropipette aspiration and to determine the effect of aging on these properties. Methods Three age groups of rabbit knees were evaluated： （1） young （2 months, n=10）; （2） adult （8 months, n=10）; and （3） old （31 months, n=10）. Chondrocytes were isolated from the right knee cartilage and chondrons were isolated from left knees using enzymatic methods. Micropipette aspiration combined with a standard linear viscoelastic solid model was used to quantify changes in the viscoelastic properties of chondrons and chondrocytes within 2 hours of isolation. The morphology and structure of isolated chondrons were evaluated by optical microscope using hematoxylin and eosin staining and collagen-6 immunofluorescence staining. Results In response to an applied constant 0.3-0.4 kPa of negative pressure, all chondrocytes exhibited standard linear viscoelastic solid properties. Model predictions of the creep data showed that the average equilibrium modulus （E~）, instantaneous modulus （E0）, and apparent viscosity （~） of old chondrocytes was significantly lower than the young and adult chondrocytes （P 〈0.001）; however, no difference was found between young and adult chondrocytes （P 〉0.05）. The adult and old chondrons generally possessed a thicker pericellular matrix （PCM） with more enclosed cells. The young and adult chondrons exhibited the same viscoelastic creep behavior under a greater applied pressure （1.0-1.1 kPa） without the deformation seen in the old chondrons. The viscoelastic properties （E,, E0, and/~） of young and adult chondrons were signifi展开更多
Objectives: To quantitatively study the adhesive pro- perties of hepatoma cells to collagen Ⅳ coated artifi- cial basement membrane and to investigate the rele- vance of cell adhesive forces to the concentration of c...Objectives: To quantitatively study the adhesive pro- perties of hepatoma cells to collagen Ⅳ coated artifi- cial basement membrane and to investigate the rele- vance of cell adhesive forces to the concentration of collagen Ⅳ. Methods: Synchronous G1 and S phase cells were a- chieved using thymine-2-desoxyriboside and cochicine sequential blockage method and double thymine-2- desoxyriboside blockage method respectively. The adhesive forces of hepatoma cells were investigated by micropipette aspiration technique. Results: The adhesive forces of hepatoma cells to ar- tificial basement membrane were (107.78±65.44) ×10^(-10)N, (182.60±107.88)×10^(-10)N, (298.91± 144.13)×10^(-10)N when the concentration of the membrane coated by 1, 2, 5μg/ml collagen Ⅳ re- spectively (P<0.001). The adhesive forces of G1 and S phases hepatoma cells to artificial basement membrane were (275.86±232.80)×10^(-10)N and (161.16±120.40)×10^(-10)N respectively when the concentration of the membrane coated by 5μg/ml collagen Ⅳ (P<0.001). Conclusions: The adhesive forces of hepatoma cells to artifical basement membrane in direct proportion to the concentration of collagen Ⅳ suggests that the in- crease of basement membrane might be conducive to the chemotactic motion and adhesiveness of tumor cells. G1 phase cells are more capable of adhering to basement membrane than S phase cells. Hepatoma cells, especially G1 phase cells, may survive in blood circulation, and sequest and adhere in microcircula- tion, and get through basement membrane for re- mote metastasis.展开更多
Using the method of gene transfection with liposome, we obtained the mouse erythro-leukemia cell line MEL-TF19, which stably carries TFAR19, a novel apoptosis-related gene. The expression of TFAR19 was detected by Wes...Using the method of gene transfection with liposome, we obtained the mouse erythro-leukemia cell line MEL-TF19, which stably carries TFAR19, a novel apoptosis-related gene. The expression of TFAR19 was detected by Western blot. Growth curve and flow cytometry analysis showed that after being transfected with TFAR19 gene, the growth of MEL-TF19 is suppressed and its apoptosis is accelerated because of the serum deprivation. Our biorheological study indi-cated that in the apoptotic process, compared with MEL cells, MEL-TF19 cells exhibit larger os-motic fragility, lower cell surface charge density, increased elastic modulus K1 which is inversely proportional to cells?maximal deformation ability, obviously diminished surface viscosity m, with elastic modulus K2 having no distinct changes. The above results provided some bases for recog-nizing the function of TFAR19 completely from the viewpoint of biorheology.展开更多
Cell adhesion plays an important role in cell physiology. A better understanding of this process could facilitate many clinical therapies. In this study, Rat bone marrow-derived mesenchymal stem cells （rBMSCs） were ...Cell adhesion plays an important role in cell physiology. A better understanding of this process could facilitate many clinical therapies. In this study, Rat bone marrow-derived mesenchymal stem cells （rBMSCs） were cultured on glass substrate, and the morphology and adhesion strength were characterized. The cell morphology was defined as spherical, adhesive, and spreading. The adhesion strengths of the different morphologies exhibited different distribu- tion patterns. The spherical cells exhibited low adhesion strength; the adhesive cells exhibited rapidly increasing adhesion strength while their diameters remained relatively constant. The ad- hesion strength increased with the cell diameter in the spreading cells. These findings suggest that adhesion strength can be quickly assessed by examining the cell morphology.展开更多
Glass micropipettes with silanized inner walls can be filled with an organic solvent for voltammetric measurements in an aqueous solution. This arrangement was employed to investigate systematically the mechanism of f...Glass micropipettes with silanized inner walls can be filled with an organic solvent for voltammetric measurements in an aqueous solution. This arrangement was employed to investigate systematically the mechanism of facilitated potassium ion transfer by an ionophore dibenzo 18 crown 6(DB18C6) across a micro water/1,2 dichloroethane(W/DCE) interface supported at the tip of a silanized micropipette. Our experimental results verify that this facilitated ion transfer across the liquid/liquid interface did occur by an interfacial complexation dissociation process(TIC TID mechanism). The ratio of the diffusion coefficient of DB18C6 to that of its complexed ion in the DCE phase was calculated to be 1.74±0.07.展开更多
Ion current rectification(ICR)is an electrodynamic phenomenon in electrolyte solution which is defined as the asymmetric potential-dependent ion flux through a confined environment,giving rise to asymmetric electrical...Ion current rectification(ICR)is an electrodynamic phenomenon in electrolyte solution which is defined as the asymmetric potential-dependent ion flux through a confined environment,giving rise to asymmetric electrical current-voltage characteristics induced by the influence of an asymmetric electrical double layer structure.Since the discovery of the ICR phenomenon,the observation and application of ICR at nanoscale and microscale have been widely investigated experimentally and theoretically.Here,the recent progress of ICR from nanoscale to microscale is systematically reviewed.Nano/micropore structures of different materials,shapes and pore sizes are first discussed.Then,the factors influencing ICRs by thermodynamically or kinetically regulating the electrical double layer structure are introduced.Moreover,theoretical models are presented to explain the mechanism of ICRs.Based on the understanding of this phenomenon,the applications,especially in biosensors,are discussed.Finally,future developments of this area are briefly presented.This review covers the representative related literature published since 2010 and is intended to give a systematic introduction to this area.展开更多
Background The mechanical properties are related with many biological functions of cells. Accurate quantification of the mechanical properties of living cells require the combined use of experimental techniques and th...Background The mechanical properties are related with many biological functions of cells. Accurate quantification of the mechanical properties of living cells require the combined use of experimental techniques and theoretical models. Micropipette aspiration (MPA) is one of common techniques in determining mechanical properties of the living cells. The halfspace model (HSM) is employed in MPA technique. However,in the conditions of linear constitutive relations and small deformations,the HSM is inadequate for characterizing the MPA of a spherical cell in two respects. Firstly,the cell size is fairly finite other than semi-infinite to the inner radius of a micropipette;Secondly,cells are compressible,with a Poisson’s ratioνvarying from 0. 2 to 0. 4 (23-25) instead of incompressible (ν=0. 5). Thus,a more accurate model is necessary.In this study,the viscoelastic expressions were derived from our previous MPA test. Then,a sphere model (SM) employed to analyze mechanical properties of rabbit chondrocytes combined with the experimental data. Differences in mechanical properties estimated by different mechanical models were evaluated.Methods A sphere model (SM) was employed. The relative dimension of cell to micropipette and the compressibility of the cell were taken into account,as shown in Fig. 1a.■Fig.1 Sphere model of the MPA of a single cell employing different constitutive relationships The approximate expression for the aspirated length was obtained from our previous study as follows:■Furthermore,assuming that the cell behaves as a homogeneous and isotropic standard linear solid (Fig. 1b),two viscoelastic creep expressions of the aspirated length for incompressible sphere model (ICSM) and for compressible sphere model (CSM) were derived by elastic-viscoelastic correspondence principle and integral transformation as Eqs.(2) and (3)respectively.■Results(1) Comparisons of models The elastic modulus from the ICSM was 47. 4%higher than that of the half-space model (HSM)(P<0. 001). For the CSM,the percentage展开更多
The erythrocytes play an important role in the human body. The healthy erythrocytes can undergo extremely large deformation while passing through small capillaries. Their infection by Malaria Plasmodium falcipurum （P...The erythrocytes play an important role in the human body. The healthy erythrocytes can undergo extremely large deformation while passing through small capillaries. Their infection by Malaria Plasmodium falcipurum （P.f.） will lead to capillary blockage and blood flow obstruction. Many experimental and computational methods have been applied to study the increase in stickiness and decrease in deformability of the Malaria （P.f.） infected erythrocytes. The novelty of this paper lies in the establishment of an multi-component model for investigating mechanical properties of Malaria （P.f.） infected erythrocytes, especially of their enclosed parasites. Finite element method was applied to simulate the erythrocytes＇ deformation in micropipette aspiration and optical tweezers stretching using the computational software ABAQUS. The comparisons between simulations and experiments were able to quantitatively conclude the effects of stiffness and stickiness of the parasitophorous vacuole membrane on the cells＇ deformation, which could not be obtained from experiments directly.展开更多
With the development of biomaterials,more attention is paid to the adhesion characteristics between cells and materials.It is necessary to study the adhesive force with a suitable method.Silk fibroin(SF)is widely inve...With the development of biomaterials,more attention is paid to the adhesion characteristics between cells and materials.It is necessary to study the adhesive force with a suitable method.Silk fibroin(SF)is widely investigated in biomedical application due to its novel biocompatibility and mechanical properties.In this article,the micropipette aspiration method and measurement pattern of uniform cells in round shape(UCR)was used to study the initial adhesive force of three types of cells on pure silk fibroin films(SFFs).We also compared the adhesive forces of modified SFFs with that of pure SFFs.The results of adhesive force in the initial adhesive stage were in concordance with the results of MTT assay andmicroscope observation,which were confirmed by the above three cell lines and four kinds of SFFs.The results indicated UCR was an efficient and quantitative measurement pattern in initial adhesion stage.This article also provides a useful method in identifying initial cell-materials interactions.展开更多
基金This study was supported by grants from the Natural Science Foundation of China (39970198) and Visiting Scholar Foundation of Education Ministry,China ([2002]4).
文摘BACKGROUND: Chemotaxis is an important step durin the invasion of carcinoma cells. And integrins are most im portant receptors mediating interaction between cells an extracellular matrix ( ECM). This study was designed t study integrin betal mediating chemotaxis of hepatocellula carcinoma (HCC) cells to laminin (LN). METHODS: A micropipette technique was adopted to in vestigate the effect of blockade of integrin betal on pseudo pod protrusion of HCC cells in response to LN stimulation Chemotactic pseudopod protrusion of a HCC cell was eva luated using a dual-pipette set-up, in which two pipette filled with LN solution were positioned in close contact wit the same cell, and pseudopod protrusion into each pipett was viewed dynamically and recorded with a tape recorder The lengths of pseudopods were measured and plotte against time to obtain a pseudopod growth curve. The in tegrin betal subunit on the surfaces of HCC cells were ana lyzed by flow cytometry. RESULTS: In dual pipette chemotaxis experiment, whe the two pipettes were filled with LN(50 μg/ml, 200 μg/ml) pseudopods extended from the HCC cell into each of th pipettes nearly symmetrically, ie, with nearly identica maximum pseudopod length and similar pseudopod growth curves. Upon addition of anti-CD29 (20 μg/ml) to one o the pipettes, pseudopod protrusion was blocked nearly completely while protrusion into the opposite pipette be came more evidently, with a larger maximum length. Ex pression of integrin betal was up to 95.78% to cells chosen in the experiment. CONCLUSION: Integrin betal subunit was an importan constituent receptor subunit for mediating chemotactic pseudopod protrusion of HCC cell to LN.
基金This work was supported in part by 973 Program （No. 2009CB526514） and National Natural Science Foundation （No. 30872616 and No. 81071495） of China.
文摘Background The mechanical microenvironment of the chondrocytes plays an important role in cartilage homeostasis and in the health of the joint. The pericellular matrix, cellular membrane of the chondrocytes, and their cytoskeletal structures are key elements in the mechanical environment. The aims of this study are to measure the viscoelastic properties of isolated chondrons and chondrocytes from rabbit knee cartilage using micropipette aspiration and to determine the effect of aging on these properties. Methods Three age groups of rabbit knees were evaluated： （1） young （2 months, n=10）; （2） adult （8 months, n=10）; and （3） old （31 months, n=10）. Chondrocytes were isolated from the right knee cartilage and chondrons were isolated from left knees using enzymatic methods. Micropipette aspiration combined with a standard linear viscoelastic solid model was used to quantify changes in the viscoelastic properties of chondrons and chondrocytes within 2 hours of isolation. The morphology and structure of isolated chondrons were evaluated by optical microscope using hematoxylin and eosin staining and collagen-6 immunofluorescence staining. Results In response to an applied constant 0.3-0.4 kPa of negative pressure, all chondrocytes exhibited standard linear viscoelastic solid properties. Model predictions of the creep data showed that the average equilibrium modulus （E~）, instantaneous modulus （E0）, and apparent viscosity （~） of old chondrocytes was significantly lower than the young and adult chondrocytes （P 〈0.001）; however, no difference was found between young and adult chondrocytes （P 〉0.05）. The adult and old chondrons generally possessed a thicker pericellular matrix （PCM） with more enclosed cells. The young and adult chondrons exhibited the same viscoelastic creep behavior under a greater applied pressure （1.0-1.1 kPa） without the deformation seen in the old chondrons. The viscoelastic properties （E,, E0, and/~） of young and adult chondrons were signifi
基金This work was supported by a grant from the National Natural Science Foundation of China (No. 39500037).
文摘Objectives: To quantitatively study the adhesive pro- perties of hepatoma cells to collagen Ⅳ coated artifi- cial basement membrane and to investigate the rele- vance of cell adhesive forces to the concentration of collagen Ⅳ. Methods: Synchronous G1 and S phase cells were a- chieved using thymine-2-desoxyriboside and cochicine sequential blockage method and double thymine-2- desoxyriboside blockage method respectively. The adhesive forces of hepatoma cells were investigated by micropipette aspiration technique. Results: The adhesive forces of hepatoma cells to ar- tificial basement membrane were (107.78±65.44) ×10^(-10)N, (182.60±107.88)×10^(-10)N, (298.91± 144.13)×10^(-10)N when the concentration of the membrane coated by 1, 2, 5μg/ml collagen Ⅳ re- spectively (P<0.001). The adhesive forces of G1 and S phases hepatoma cells to artificial basement membrane were (275.86±232.80)×10^(-10)N and (161.16±120.40)×10^(-10)N respectively when the concentration of the membrane coated by 5μg/ml collagen Ⅳ (P<0.001). Conclusions: The adhesive forces of hepatoma cells to artifical basement membrane in direct proportion to the concentration of collagen Ⅳ suggests that the in- crease of basement membrane might be conducive to the chemotactic motion and adhesiveness of tumor cells. G1 phase cells are more capable of adhering to basement membrane than S phase cells. Hepatoma cells, especially G1 phase cells, may survive in blood circulation, and sequest and adhere in microcircula- tion, and get through basement membrane for re- mote metastasis.
基金supported by the Key Project of the National Natural Science Foundation of China(Grant No.39830110)the Doctoral Foundation(Grant No.20010001082).
文摘Using the method of gene transfection with liposome, we obtained the mouse erythro-leukemia cell line MEL-TF19, which stably carries TFAR19, a novel apoptosis-related gene. The expression of TFAR19 was detected by Western blot. Growth curve and flow cytometry analysis showed that after being transfected with TFAR19 gene, the growth of MEL-TF19 is suppressed and its apoptosis is accelerated because of the serum deprivation. Our biorheological study indi-cated that in the apoptotic process, compared with MEL cells, MEL-TF19 cells exhibit larger os-motic fragility, lower cell surface charge density, increased elastic modulus K1 which is inversely proportional to cells?maximal deformation ability, obviously diminished surface viscosity m, with elastic modulus K2 having no distinct changes. The above results provided some bases for recog-nizing the function of TFAR19 completely from the viewpoint of biorheology.
基金financial support received from the Sate Key Laboratory of Tribology, Tsinghua University
文摘Cell adhesion plays an important role in cell physiology. A better understanding of this process could facilitate many clinical therapies. In this study, Rat bone marrow-derived mesenchymal stem cells （rBMSCs） were cultured on glass substrate, and the morphology and adhesion strength were characterized. The cell morphology was defined as spherical, adhesive, and spreading. The adhesion strengths of the different morphologies exhibited different distribu- tion patterns. The spherical cells exhibited low adhesion strength; the adhesive cells exhibited rapidly increasing adhesion strength while their diameters remained relatively constant. The ad- hesion strength increased with the cell diameter in the spreading cells. These findings suggest that adhesion strength can be quickly assessed by examining the cell morphology.
基金Supported by Chinese Academ y of Sciences(CAS) National Natural Science Foundation of China(No. 2 982 5 111) andthe Third World Academ y of Science(TWAS) and the State Key L aboratory of Electroanalytical Chem istry of the CAS.
文摘Glass micropipettes with silanized inner walls can be filled with an organic solvent for voltammetric measurements in an aqueous solution. This arrangement was employed to investigate systematically the mechanism of facilitated potassium ion transfer by an ionophore dibenzo 18 crown 6(DB18C6) across a micro water/1,2 dichloroethane(W/DCE) interface supported at the tip of a silanized micropipette. Our experimental results verify that this facilitated ion transfer across the liquid/liquid interface did occur by an interfacial complexation dissociation process(TIC TID mechanism). The ratio of the diffusion coefficient of DB18C6 to that of its complexed ion in the DCE phase was calculated to be 1.74±0.07.
基金supported by the National Natural Science Foundation of China (21775151, 21790053, 21475138 for P.Y.,21790390, 21790391, 21435007, 21621062 for L.M.)the National Basic Research Program of China (2016YFA0200104)+1 种基金the Strategic Priority Research Program of Chinese Academy of Sciences (XDB30000000)the Chinese Academy of Sciences (QYZDJSSW-SLH030)
文摘Ion current rectification(ICR)is an electrodynamic phenomenon in electrolyte solution which is defined as the asymmetric potential-dependent ion flux through a confined environment,giving rise to asymmetric electrical current-voltage characteristics induced by the influence of an asymmetric electrical double layer structure.Since the discovery of the ICR phenomenon,the observation and application of ICR at nanoscale and microscale have been widely investigated experimentally and theoretically.Here,the recent progress of ICR from nanoscale to microscale is systematically reviewed.Nano/micropore structures of different materials,shapes and pore sizes are first discussed.Then,the factors influencing ICRs by thermodynamically or kinetically regulating the electrical double layer structure are introduced.Moreover,theoretical models are presented to explain the mechanism of ICRs.Based on the understanding of this phenomenon,the applications,especially in biosensors,are discussed.Finally,future developments of this area are briefly presented.This review covers the representative related literature published since 2010 and is intended to give a systematic introduction to this area.
文摘Background The mechanical properties are related with many biological functions of cells. Accurate quantification of the mechanical properties of living cells require the combined use of experimental techniques and theoretical models. Micropipette aspiration (MPA) is one of common techniques in determining mechanical properties of the living cells. The halfspace model (HSM) is employed in MPA technique. However,in the conditions of linear constitutive relations and small deformations,the HSM is inadequate for characterizing the MPA of a spherical cell in two respects. Firstly,the cell size is fairly finite other than semi-infinite to the inner radius of a micropipette;Secondly,cells are compressible,with a Poisson’s ratioνvarying from 0. 2 to 0. 4 (23-25) instead of incompressible (ν=0. 5). Thus,a more accurate model is necessary.In this study,the viscoelastic expressions were derived from our previous MPA test. Then,a sphere model (SM) employed to analyze mechanical properties of rabbit chondrocytes combined with the experimental data. Differences in mechanical properties estimated by different mechanical models were evaluated.Methods A sphere model (SM) was employed. The relative dimension of cell to micropipette and the compressibility of the cell were taken into account,as shown in Fig. 1a.■Fig.1 Sphere model of the MPA of a single cell employing different constitutive relationships The approximate expression for the aspirated length was obtained from our previous study as follows:■Furthermore,assuming that the cell behaves as a homogeneous and isotropic standard linear solid (Fig. 1b),two viscoelastic creep expressions of the aspirated length for incompressible sphere model (ICSM) and for compressible sphere model (CSM) were derived by elastic-viscoelastic correspondence principle and integral transformation as Eqs.(2) and (3)respectively.■Results(1) Comparisons of models The elastic modulus from the ICSM was 47. 4%higher than that of the half-space model (HSM)(P<0. 001). For the CSM,the percentage
基金supported by the National Natural Science Foundation of China (11072178,11172214)the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry,and Shanghai Leading Academic Discipline Project (B302)
文摘The erythrocytes play an important role in the human body. The healthy erythrocytes can undergo extremely large deformation while passing through small capillaries. Their infection by Malaria Plasmodium falcipurum （P.f.） will lead to capillary blockage and blood flow obstruction. Many experimental and computational methods have been applied to study the increase in stickiness and decrease in deformability of the Malaria （P.f.） infected erythrocytes. The novelty of this paper lies in the establishment of an multi-component model for investigating mechanical properties of Malaria （P.f.） infected erythrocytes, especially of their enclosed parasites. Finite element method was applied to simulate the erythrocytes＇ deformation in micropipette aspiration and optical tweezers stretching using the computational software ABAQUS. The comparisons between simulations and experiments were able to quantitatively conclude the effects of stiffness and stickiness of the parasitophorous vacuole membrane on the cells＇ deformation, which could not be obtained from experiments directly.
基金This work was supported by the National Natural Science Foundation of China(NSFC,no.51502192,11502158,31501212 and 51503140)Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(STIP,no.2016142)+4 种基金National Basic Research Program of China(973 project,2005CB623906)Natural Science Foundation for Young Scientists of Shanxi Province(no.2014021039-6 and 201601D021127)the Qualified Personnel Foundation of Taiyuan University of Technology(QPFT,no.tyut-rc201270a)the Youth Foundation of Taiyuan University of Technology(no.1205-04020102,2013Z020 and 2014TD066)the Technical Services Project of Taiyuan University of Technology(no.143230043-J).
文摘With the development of biomaterials,more attention is paid to the adhesion characteristics between cells and materials.It is necessary to study the adhesive force with a suitable method.Silk fibroin(SF)is widely investigated in biomedical application due to its novel biocompatibility and mechanical properties.In this article,the micropipette aspiration method and measurement pattern of uniform cells in round shape(UCR)was used to study the initial adhesive force of three types of cells on pure silk fibroin films(SFFs).We also compared the adhesive forces of modified SFFs with that of pure SFFs.The results of adhesive force in the initial adhesive stage were in concordance with the results of MTT assay andmicroscope observation,which were confirmed by the above three cell lines and four kinds of SFFs.The results indicated UCR was an efficient and quantitative measurement pattern in initial adhesion stage.This article also provides a useful method in identifying initial cell-materials interactions.
