The ongoing coronavirus disease 2019(COVID-19) pandemic has boosted the development of antiviral research.Microfluidic technologies offer powerful platforms for diagnosis and drug discovery for severe acute respirator...The ongoing coronavirus disease 2019(COVID-19) pandemic has boosted the development of antiviral research.Microfluidic technologies offer powerful platforms for diagnosis and drug discovery for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) diagnosis and drug discovery.In this review,we introduce the structure of SARS-CoV-2 and the basic knowledge of microfluidic design.We discuss the application of microfluidic devices in SARS-CoV-2 diagnosis based on detecting viral nucleic acid,antibodies,and antigens.We highlight the contribution of lab-on-a-chip to manufacturing point-ofcare equipment of accurate,sensitive,low-cost,and user-friendly virus-detection devices.We then investigate the efforts in organ-on-a-chip and lipid nanoparticles(LNPs) synthesizing chips in antiviral drug screening and mRNA vaccine preparation.Microfluidic technologies contribute to the ongoing SARSCoV-2 research efforts and provide tools for future viral outbreaks.展开更多
A combined detection system involving simultaneous LIF and contacfless-conductometric measurements at the same place of the microfluidic chip was described. The LIF measurement was designed according to the confocal p...A combined detection system involving simultaneous LIF and contacfless-conductometric measurements at the same place of the microfluidic chip was described. The LIF measurement was designed according to the confocal principle and a moveable contactless-conduetivity detector was used in C^4D. Both measurements were mutually independent and advantageous in analyses of mixtures. Various experimental parameters affecting the response were examined and optimized. The performances were demonstrated by simultaneous detection of Rhodamine B. And the results showed that the combined detection system could be used sensitively and reliably.展开更多
A PDMS electrophoresis microchip,which integrated with optical fiber for fluorescence detection,was fabricated by using silicon master.A deep reactive ion etches (DRIE) technology was used to fabricate the silicon ma...A PDMS electrophoresis microchip,which integrated with optical fiber for fluorescence detection,was fabricated by using silicon master.A deep reactive ion etches (DRIE) technology was used to fabricate the silicon master with positive features.The PDMS replica was fabricated by casting PDMS prepolymer against the silicon master,where an optical fiber was first fixed on the end of separation microchannel.To improve the rigid characteristics of integrated PDMS microchip,the chips were subsequently assembled by reversible sealing against glass plate.A blue light emitting diode (LED) was used as excitation light sources for inducing fluorescence detection through coupling LED light into the optical fiber.As an application, integrated PDMS microchip was tested in the capillary electrophoresis separation of DNA markers.The results showed that DNA markers could be effectively separated and detected except for the segments of 271 and 281.展开更多
An on-chip electrochemical detector for microfluidic chips was described, based on integrated carbon nanotube （CNT） electrodes directly onto the chip substrate through microwave plasma chemical vapor deposition （MW...An on-chip electrochemical detector for microfluidic chips was described, based on integrated carbon nanotube （CNT） electrodes directly onto the chip substrate through microwave plasma chemical vapor deposition （MWPCVD）. The attractive performance of the integrated CNT electrodes was demonstrated for the amperometric detection of sucrose, glucose and D-fructose. The integrated CNT electrodes showed stronger electrocatalytic activity than gold electrodes.展开更多
基金support from the National Natural Science Foundation of China(82072087,31970893,32270976)funding by Science and Technology Projects in Guangzhou(202206010087,China)。
文摘The ongoing coronavirus disease 2019(COVID-19) pandemic has boosted the development of antiviral research.Microfluidic technologies offer powerful platforms for diagnosis and drug discovery for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) diagnosis and drug discovery.In this review,we introduce the structure of SARS-CoV-2 and the basic knowledge of microfluidic design.We discuss the application of microfluidic devices in SARS-CoV-2 diagnosis based on detecting viral nucleic acid,antibodies,and antigens.We highlight the contribution of lab-on-a-chip to manufacturing point-ofcare equipment of accurate,sensitive,low-cost,and user-friendly virus-detection devices.We then investigate the efforts in organ-on-a-chip and lipid nanoparticles(LNPs) synthesizing chips in antiviral drug screening and mRNA vaccine preparation.Microfluidic technologies contribute to the ongoing SARSCoV-2 research efforts and provide tools for future viral outbreaks.
文摘A combined detection system involving simultaneous LIF and contacfless-conductometric measurements at the same place of the microfluidic chip was described. The LIF measurement was designed according to the confocal principle and a moveable contactless-conduetivity detector was used in C^4D. Both measurements were mutually independent and advantageous in analyses of mixtures. Various experimental parameters affecting the response were examined and optimized. The performances were demonstrated by simultaneous detection of Rhodamine B. And the results showed that the combined detection system could be used sensitively and reliably.
基金This present work was supported by grant from the National Natural Science Foundation of China (N0．60501020， No．60341005 and No．20299030)
文摘A PDMS electrophoresis microchip,which integrated with optical fiber for fluorescence detection,was fabricated by using silicon master.A deep reactive ion etches (DRIE) technology was used to fabricate the silicon master with positive features.The PDMS replica was fabricated by casting PDMS prepolymer against the silicon master,where an optical fiber was first fixed on the end of separation microchannel.To improve the rigid characteristics of integrated PDMS microchip,the chips were subsequently assembled by reversible sealing against glass plate.A blue light emitting diode (LED) was used as excitation light sources for inducing fluorescence detection through coupling LED light into the optical fiber.As an application, integrated PDMS microchip was tested in the capillary electrophoresis separation of DNA markers.The results showed that DNA markers could be effectively separated and detected except for the segments of 271 and 281.
基金Supported by National Natural Science Foundation of China (Nos.50572075, 51072140)the Young and Middle-Aged Elitists' Scientific and Technological Innovation Team Project of the Institutions of Higher Education in Hubei Province of China the Scientific Research Projects of Hubei Education Department of China (Z200715001)
文摘An on-chip electrochemical detector for microfluidic chips was described, based on integrated carbon nanotube （CNT） electrodes directly onto the chip substrate through microwave plasma chemical vapor deposition （MWPCVD）. The attractive performance of the integrated CNT electrodes was demonstrated for the amperometric detection of sucrose, glucose and D-fructose. The integrated CNT electrodes showed stronger electrocatalytic activity than gold electrodes.
