Normally,proper fermentation can be an efficient and widely used method to improve feed quality in animal rearing;however,the studies on crustaceans,especially Eriocheir sinensis,remain limited.This study aimed to inv...Normally,proper fermentation can be an efficient and widely used method to improve feed quality in animal rearing;however,the studies on crustaceans,especially Eriocheir sinensis,remain limited.This study aimed to investigate whether feed fermentation could meliorate dietary nutritional value and benefit E.sinensis rearing.First,non-fermented feed(NFD)and fermented feed(FD)were produced and assessed,respectively.Then,the“Y”maze feed choice behavior test(180 times;30 times,6 rounds)was conducted to assess the attractiveness of these 2 feeds for crabs.Finally,a total of 80 crabs(44.10±0.80 g)were randomly assigned into 2 groups with 4 replicates,and fed the experimental diets for 8 weeks to evaluate the effects of each feed on growth,antioxidant capacity,meat flavor,and intestinal microbiota.In this study,FD showed higher levels of crude protein(P<0.01),soluble protein(P<0.01),amino acids(P<0.05),lactic acid(P<0.001),and lower levels of crude fiber(P<0.05)and antinutritional factors(agglutinin,trypsin inhibitor,glycinin,andβ-conglycinin)(P<0.001)than NFD.Additionally,FD was more attractive to crabs than NFD(P<0.01)and it stimulated the appetite of crabs more than NFD(P<0.05).The growth performance,feed efficiency,and digestive enzyme activity of FDfed crabs were significantly higher than those of NFD-fed crabs(P<0.05).The electronic sensory measurements and free amino acid profiles revealed that the FD diet had positive impacts on the meat flavor of crabs,particularly in“sweet”and“umami”tastes.Moreover,the antioxidant capacity of FD-fed crabs was significantly higher than that of NFD-fed crabs(P<0.05).Fermented feed also affected the diversity and composition of intestinal microflora.The functional prediction of microbial communities showed that crabs fed FD had a better microecological environment in the intestine.In conclusion,the fermentation of aquafeed could be an effective approach to enhance feed quality and therefore benefit E.sinensis rearing.展开更多
应用模拟人体胃肠消化的SHIME(simulator of the human intestinal microbial ecosystem)体外方法评价肠道菌群对不同农产品中腐霉利生物可给性的作用规律,为正确评价农产品中农药残留健康风险提供理论依据。结果表明:肠道菌群可使葡萄...应用模拟人体胃肠消化的SHIME(simulator of the human intestinal microbial ecosystem)体外方法评价肠道菌群对不同农产品中腐霉利生物可给性的作用规律,为正确评价农产品中农药残留健康风险提供理论依据。结果表明:肠道菌群可使葡萄等13种农产品中腐霉利生物可给性降低30.3%~97.2%,其中以葡萄和大豆中供试农药残留生物可给性的影响较为显著;膳食成分可增强肠道菌群对腐霉利生物可给性的影响,菊粉作用下可使肠道菌群对葡萄中腐霉利生物可给性降低39.6%。大肠杆菌是影响腐霉利生物可给性的主要优势菌,36 h时可使葡萄中腐霉利生物可给性显著降低33.9%。展开更多
基金funded by the Open Competition Project for Jiangsu Seed Industry Vitalization(Grant No.JBGS[2021]129)by Major Project of Modern Agriculture of Jiangsu Key R&D(Grant No.BE2022366)。
文摘Normally,proper fermentation can be an efficient and widely used method to improve feed quality in animal rearing;however,the studies on crustaceans,especially Eriocheir sinensis,remain limited.This study aimed to investigate whether feed fermentation could meliorate dietary nutritional value and benefit E.sinensis rearing.First,non-fermented feed(NFD)and fermented feed(FD)were produced and assessed,respectively.Then,the“Y”maze feed choice behavior test(180 times;30 times,6 rounds)was conducted to assess the attractiveness of these 2 feeds for crabs.Finally,a total of 80 crabs(44.10±0.80 g)were randomly assigned into 2 groups with 4 replicates,and fed the experimental diets for 8 weeks to evaluate the effects of each feed on growth,antioxidant capacity,meat flavor,and intestinal microbiota.In this study,FD showed higher levels of crude protein(P<0.01),soluble protein(P<0.01),amino acids(P<0.05),lactic acid(P<0.001),and lower levels of crude fiber(P<0.05)and antinutritional factors(agglutinin,trypsin inhibitor,glycinin,andβ-conglycinin)(P<0.001)than NFD.Additionally,FD was more attractive to crabs than NFD(P<0.01)and it stimulated the appetite of crabs more than NFD(P<0.05).The growth performance,feed efficiency,and digestive enzyme activity of FDfed crabs were significantly higher than those of NFD-fed crabs(P<0.05).The electronic sensory measurements and free amino acid profiles revealed that the FD diet had positive impacts on the meat flavor of crabs,particularly in“sweet”and“umami”tastes.Moreover,the antioxidant capacity of FD-fed crabs was significantly higher than that of NFD-fed crabs(P<0.05).Fermented feed also affected the diversity and composition of intestinal microflora.The functional prediction of microbial communities showed that crabs fed FD had a better microecological environment in the intestine.In conclusion,the fermentation of aquafeed could be an effective approach to enhance feed quality and therefore benefit E.sinensis rearing.
文摘应用模拟人体胃肠消化的SHIME(simulator of the human intestinal microbial ecosystem)体外方法评价肠道菌群对不同农产品中腐霉利生物可给性的作用规律,为正确评价农产品中农药残留健康风险提供理论依据。结果表明:肠道菌群可使葡萄等13种农产品中腐霉利生物可给性降低30.3%~97.2%,其中以葡萄和大豆中供试农药残留生物可给性的影响较为显著;膳食成分可增强肠道菌群对腐霉利生物可给性的影响,菊粉作用下可使肠道菌群对葡萄中腐霉利生物可给性降低39.6%。大肠杆菌是影响腐霉利生物可给性的主要优势菌,36 h时可使葡萄中腐霉利生物可给性显著降低33.9%。
