[目的]探讨3种微生物多糖对草地早熟禾抗旱胁迫下抗性酶系及生理生化指标的影响。[方法]采用微生物多糖均匀喷施草坪草,根据高渗溶液胁迫法,喷施100 m L 20%聚乙二醇,干旱胁迫7天后,测定相关生理指标。[结果]酵母多糖J和细菌多糖X对抗...[目的]探讨3种微生物多糖对草地早熟禾抗旱胁迫下抗性酶系及生理生化指标的影响。[方法]采用微生物多糖均匀喷施草坪草,根据高渗溶液胁迫法,喷施100 m L 20%聚乙二醇,干旱胁迫7天后,测定相关生理指标。[结果]酵母多糖J和细菌多糖X对抗性酶系和生理指标均有影响,酵母多糖J对POD活性、SOD活性、SS含量比CK高2.56、2.13和4.54倍,细菌多糖X对CAT活性、Pro含量、SP含量比CK高3.15、5.26和2.46倍,而真菌多糖D在降低MDA活性上效果最佳,比CK低53.6%。[结论]为开发安全、绿色的微生物多糖制剂奠定理论基础。展开更多
The objective of this study was to investigate the sulfonation of (1→6)-β-D-glucan (lasiodiplodan) as a potentiating mechanism for biological functionalities. Lasiodiplodan was sulfonated by the chlorosulfonic a...The objective of this study was to investigate the sulfonation of (1→6)-β-D-glucan (lasiodiplodan) as a potentiating mechanism for biological functionalities. Lasiodiplodan was sulfonated by the chlorosulfonic acid-pyridine method. The modified exopolysaccharide was characterized by FT-IR and 13C NMR spectroscopy, X-ray diffraction and SEM. Antioxidant activity was assessed by the methods of H2O2 and OH radical removal and reducing power. Antimicrobial potential was evaluated by the broth-microdilution method. Sulfonation resulted in a derivative with DS of 0.24. FT-IR analysis indicated the introduction of sulfonyl groups in the macromolecule structure through specific bands in the regions of 1,240 cm-1 and 810 cm-1. 13C NMR analysis suggested that sulfonation occurred at carbon 2 of the glucose residue. Sulfonation led to morphological changes in the structure of the biopolymer resulting in a heterogeneous structure with the presence of fibrils. Derivatization promoted an increase in the antioxidant ability of the macromolecule, with a high OH removal potential (74.32%). Bacteriostatic activity against E. coli (Escherichia coli) and S. enterica (Salmonella enterica) typhimurium and fungicidal activity against C. albicans (Candida albicans) and C. tropicalis (Candida tropicalis) were found in the sulfonated sample. Sulfonation potentiated the antioxidant and antimicrobial activities of the biomacromolecule, suggesting that it is a potentiating mechanism of biological functions.展开更多
Lignocellulose—a major component of biomass available on earth is a renewable and abundantly available with great potential for bioconversion to value-added bio-products. The review aims at physio-chemical features o...Lignocellulose—a major component of biomass available on earth is a renewable and abundantly available with great potential for bioconversion to value-added bio-products. The review aims at physio-chemical features of lignocellulosic biomass and composition of different lignocellulosic materials. This work is an overview about the conversion of lignocellulosic biomass into bio-energy products such as bio-ethanol, 1-butanol, bio-methane, bio-hydrogen, organic acids including citric acid, succinic acid and lactic acid, microbial polysaccharides, single cell protein and xylitol. The biotechnological aspect of bio-transformation of lignocelluloses research and its future prospects are also discussed.展开更多
文摘[目的]探讨3种微生物多糖对草地早熟禾抗旱胁迫下抗性酶系及生理生化指标的影响。[方法]采用微生物多糖均匀喷施草坪草,根据高渗溶液胁迫法,喷施100 m L 20%聚乙二醇,干旱胁迫7天后,测定相关生理指标。[结果]酵母多糖J和细菌多糖X对抗性酶系和生理指标均有影响,酵母多糖J对POD活性、SOD活性、SS含量比CK高2.56、2.13和4.54倍,细菌多糖X对CAT活性、Pro含量、SP含量比CK高3.15、5.26和2.46倍,而真菌多糖D在降低MDA活性上效果最佳,比CK低53.6%。[结论]为开发安全、绿色的微生物多糖制剂奠定理论基础。
文摘The objective of this study was to investigate the sulfonation of (1→6)-β-D-glucan (lasiodiplodan) as a potentiating mechanism for biological functionalities. Lasiodiplodan was sulfonated by the chlorosulfonic acid-pyridine method. The modified exopolysaccharide was characterized by FT-IR and 13C NMR spectroscopy, X-ray diffraction and SEM. Antioxidant activity was assessed by the methods of H2O2 and OH radical removal and reducing power. Antimicrobial potential was evaluated by the broth-microdilution method. Sulfonation resulted in a derivative with DS of 0.24. FT-IR analysis indicated the introduction of sulfonyl groups in the macromolecule structure through specific bands in the regions of 1,240 cm-1 and 810 cm-1. 13C NMR analysis suggested that sulfonation occurred at carbon 2 of the glucose residue. Sulfonation led to morphological changes in the structure of the biopolymer resulting in a heterogeneous structure with the presence of fibrils. Derivatization promoted an increase in the antioxidant ability of the macromolecule, with a high OH removal potential (74.32%). Bacteriostatic activity against E. coli (Escherichia coli) and S. enterica (Salmonella enterica) typhimurium and fungicidal activity against C. albicans (Candida albicans) and C. tropicalis (Candida tropicalis) were found in the sulfonated sample. Sulfonation potentiated the antioxidant and antimicrobial activities of the biomacromolecule, suggesting that it is a potentiating mechanism of biological functions.
文摘Lignocellulose—a major component of biomass available on earth is a renewable and abundantly available with great potential for bioconversion to value-added bio-products. The review aims at physio-chemical features of lignocellulosic biomass and composition of different lignocellulosic materials. This work is an overview about the conversion of lignocellulosic biomass into bio-energy products such as bio-ethanol, 1-butanol, bio-methane, bio-hydrogen, organic acids including citric acid, succinic acid and lactic acid, microbial polysaccharides, single cell protein and xylitol. The biotechnological aspect of bio-transformation of lignocelluloses research and its future prospects are also discussed.