Phytochromes in seed plants are known to move into nuclei in a red light-dependent manner with or without interacting factors. Here, we show phytochrome relocation to the nuclear region in phytochrome-dependent Adiant...Phytochromes in seed plants are known to move into nuclei in a red light-dependent manner with or without interacting factors. Here, we show phytochrome relocation to the nuclear region in phytochrome-dependent Adiantum capillus-veneris spore germination by partial spore-irradiation experiments. The nuclear or non-nuclear region of imbibed spores was irradiated with a microbeam of red and/or far-red light and the localization of phytochrome involved in spore germination was estimated from the germination rate. The phytochrome for spore germination existed throughout whole spore under darkness after imbibition, but gradually migrated to the nuclear region following red light irradiation. In- tracellular distribution of PHY-GUS fusion proteins expressed in germinated spores by particle bombardment showed the migration of Acphy2, but not Acphyl, into nucleus in a red light-dependent manner, suggesting that Acphy2 is the photoreceptor for fern spore germination.展开更多
We present a new analytical model for electrostatically actuated microbeams to explore the size effect by using the modified couple stress theory and the minimum total potential energy principle. A material length sca...We present a new analytical model for electrostatically actuated microbeams to explore the size effect by using the modified couple stress theory and the minimum total potential energy principle. A material length scale parameter is introduced to represent the size-dependent characteristics of microbeams. This model also accounts for the nonlinearities associated with the mid-plane stretching force and the electrostatical force. Numerical analysis for microbeams with clamped-clamped and cantilevered conditions has been performed. It is found that the intensity of size effect is closely associated with the thickness of the microbeam,and smaller beam thickness displays stronger size effect and hence yields smaller deffection and larger pull-in voltage. When the beam thickness is comparable to the material length scale parameter,the size effect is significant and the present theoretical model including the material length scale parameter is adequate for predicting the static behavior of microbeam-based MEMS.展开更多
The classical piezoelectric theory fails to capture the size-dependent electromechanical coupling behaviors of piezoelectric microstructures due to the lack of material length-scale parameters.This study presents the ...The classical piezoelectric theory fails to capture the size-dependent electromechanical coupling behaviors of piezoelectric microstructures due to the lack of material length-scale parameters.This study presents the constitutive relations of a piezoelectric material in terms of irreducible transversely isotropic tensors that include material length-scale parameters.Using these relations and the general strain gradient theory,a size-dependent bending model is proposed for a bilayer cantilever microbeam consisting of a transversely isotropic piezoelectric layer and an isotropic elastic layer.Analytical solutions are provided for bilayer cantilever microbeams subjected to force load and voltage load.The proposed model can be simplified to the model incorporating only partial strain gradient effects.This study examines the effect of strain gradient by comparing the normalized electric potentials and deflections of different models.Numerical results show that the proposed model effectively captures size effects in piezoelectric microbeams,whereas simplified models underestimate size effects due to ignoring partial strain gradient effects.展开更多
Using cotyledonary petioles as explants, the PAP cDNA controlled by wound-in-ducible promoter has been introduced into Brassica napus by coculture with Agrobacterium tumefaciens and laser microbeam puncture respective...Using cotyledonary petioles as explants, the PAP cDNA controlled by wound-in-ducible promoter has been introduced into Brassica napus by coculture with Agrobacterium tumefaciens and laser microbeam puncture respectively. Regenerated plants resistant to gen-tamycin have been selected out. PCR amplification and Southern blotting analysis indicated that PAP cDNA together with wound-inducible promoter had been integrated into Brassica genome with transformation frequencies of 2.0% and 1.7% for two transformation methods respectively. The test of virus challenge showed that these transgenic Brassica plants were resistant in different degrees to mechanically inoculated TuMV.展开更多
The objective of this paper is to model the size-dependent thermo-mechanical behaviors of a shape memory polymer (SMP) microbeam.Size-dependent constitutive equations,which can capture the size effect of the SMP,are p...The objective of this paper is to model the size-dependent thermo-mechanical behaviors of a shape memory polymer (SMP) microbeam.Size-dependent constitutive equations,which can capture the size effect of the SMP,are proposed based on the modified couple stress theory (MCST).The deformation energy expression of the SMP microbeam is obtained by employing the proposed size-dependent constitutive equation and Bernoulli-Euler beam theory.An SMP microbeam model,which includes the formulations of deflection,strain,curvature,stress and couple stress,is developed by using the principle of minimum potential energy and the separation of variables together.The sizedependent thermo-mechanical and shape memory behaviors of the SMP microbeam and the influence of the Poisson ratio are numerically investigated according to the developed SMP microbeam model.Results show that the size effects of the SMP microbeam are significant when the dimensionless height is small enough.However,they are too slight to be necessarily considered when the dimensionless height is large enough.The bending flexibility and stress level of the SMP microbeam rise with the increasing dimensionless height,while the couple stress level declines with the increasing dimensionless height.The larger the dimensionless height is,the more obvious the viscous property and shape memory effect of the SMP microbeam are.The Poisson ratio has obvious influence on the size-dependent behaviors of the SMP microbeam.The paper provides a theoretical basis and a quantitatively analyzing tool for the design and analysis of SMP micro-structures in the field of biological medicine,microelectronic devices and micro-electro-mechanical system (MEMS) self-assembling.展开更多
On the basis of the Euler-Bernoulli hypothesis, nonlinear static and dynamic responses of a viscoelastic microbeam under two kinds of electric forces [a purely direct current （DC） and a combined current composed of ...On the basis of the Euler-Bernoulli hypothesis, nonlinear static and dynamic responses of a viscoelastic microbeam under two kinds of electric forces [a purely direct current （DC） and a combined current composed of a DC and an alternating current] are studied. By using Taylor series expansion, a governing equation of nonlinear integro-differential type is derived, and numerical analyses are performed. When a purely DC is applied, there exist an instantaneous pull-in voltage and a durable pull-in voltage of which the physical meanings are also given, whereas under an applied combined current, the effect of the element relaxation coefficient on the dynamic pull-in phenomenon is observed where the largest Lyapunov exponent is taken as a criterion for the dynamic pull-in instability of viscoelastic microbeams.展开更多
In this paper the single-event responses of the silicon germanium heterojunction bipolar transistors(SiGe HBTs) are investigated by TCAD simulations and laser microbeam experiment. A three-dimensional(3D) simulation m...In this paper the single-event responses of the silicon germanium heterojunction bipolar transistors(SiGe HBTs) are investigated by TCAD simulations and laser microbeam experiment. A three-dimensional(3D) simulation model is established, the single event effect(SEE) simulation is further carried out on the basis of Si Ge HBT devices, and then, together with the laser microbeam test, the charge collection behaviors are analyzed, including the single event transient(SET) induced transient terminal currents, and the sensitive area of SEE charge collection. The simulations and experimental results are discussed in detail and it is demonstrated that the nature of the current transient is controlled by the behaviors of the collector–substrate(C/S) junction and charge collection by sensitive electrodes, thereby giving out the sensitive area and electrode of SiGe HBT in SEE.展开更多
A method for microdissection, isolation and amplification of plant chromosomal fragments using laser microbeam and a glass microneedle was established. Firstly, 7H chromosome of barley (Hordeum vulgare L.) was dissect...A method for microdissection, isolation and amplification of plant chromosomal fragments using laser microbeam and a glass microneedle was established. Firstly, 7H chromosome of barley (Hordeum vulgare L.) was dissected by Nd: YAG laserbeam with suitable parameters and the fragment comprising a satellite was isolated with a glass microneedle which was fixed on a micromanipulator. Then, the chromosomal fragment DNA was amplified by LA_PCR (linker adaptor PCR) for two rounds. The size of the DNA fragments of PCR products varied from 500-3 000 bp and the PCR products originated from the genome of barley were verified by Southern hybridization. Compared with previous reports, there are some advantages in this research. The performance is easier, the dissection is more precise and the cost is low. It also permits efficient amplification with only one single chromosome fragment. Laser microbeam_glass microneedle method may be useful in the microdissection of special chromosome regions, especially in plants with middle or small chromosomes.展开更多
Laser tweezers can manipulate small particles, such as cells and organelles. When coupling them with laser microbeam selective fusion of two tobacco protoplasts containing some chloroplast was achieved. Physical and b...Laser tweezers can manipulate small particles, such as cells and organelles. When coupling them with laser microbeam selective fusion of two tobacco protoplasts containing some chloroplast was achieved. Physical and biological variables that affect laser trapping and laser-induced fusion were also discussed. The results show that the effect of chloroplast content and distribution on the yield of cell fusion is remarkable.展开更多
High-energy proton microbeam facilities are powerful tools in space science,biology and cancer therapy studies.The primary limitations of the 50 MeV proton microbeam system are the poor beam quality provided by the cy...High-energy proton microbeam facilities are powerful tools in space science,biology and cancer therapy studies.The primary limitations of the 50 MeV proton microbeam system are the poor beam quality provided by the cyclotron and the problem of intense scattering in the slit position.Here,we present an optical design for a cyclotron-based 50 MeV high-energy proton microbeam system with a micron-sized resolution.The microbeam system,which has an Oxford triplet lens configuration,has relatively small spherical aberrations and is insensitive to changes in the beam divergence angle and momentum spread.In addition,the energy filtration included in the system can reduce the beam momentum spread from 1 to 0.02%.The effects of lens parasitic aberrations and the lens fringe field on the beam spot resolution are also discussed.In addition,owing to the severe scattering of 50 MeV protons in slit materials,a slit system model based on the Geant4 toolkit enables the quantitative analysis of scattered protons and secondary particles.For the slit system settings under a 10-micron final beam spot,very few scattered protons can enter the quadrupole lens system and affect the focusing performance of the microbeam system,but the secondary radiation of neutrons and gamma rays generated at the collimation system should be considered for the 50 MeV proton microbeam.These data demonstrate that a 50 MeV proton microbeam system with a micron-sized beam spot based on a cyclotron is feasible.展开更多
In this manuscript,we study a new version of the optical recursional binormal microbeam model for a flexible binormal microscale beam in terms of a binormal normalized operator.Also,we give new explanations for the op...In this manuscript,we study a new version of the optical recursional binormal microbeam model for a flexible binormal microscale beam in terms of a binormal normalized operator.Also,we give new explanations for the optical recursional visco Landau-Lifshitz binormal electromagnetic binormal microscale beam.Finally,we obtain an optical application for the normalized visco Landau-Lifshitz electromagnetic binormal optimistic density with an optical binormal resonator.展开更多
In extreme heat transfer environments, functionally graded materials(FGMs)have aroused great concern due to the excellent thermal shock resistance. With the development of micro-scale devices, the size-dependent effec...In extreme heat transfer environments, functionally graded materials(FGMs)have aroused great concern due to the excellent thermal shock resistance. With the development of micro-scale devices, the size-dependent effect has become an important issue. However, the classical continuum mechanical model fails on the micro-scale due to the influence of the size-dependent effect. Meanwhile, for thermoelastic behaviors limited to small-scale problems, Fourier's heat conduction law cannot explain the thermal wave effect. In order to capture the size-dependent effect and the thermal wave effect, the nonlocal generalized thermoelastic theory for the formulation of an FGM microbeam is adopted in the present work. For numerical validation, the transient responses for a simply supported FGM microbeam heated by the ramp-type heating are considered.The governing equations are formulated and solved by employing the Laplace transform techniques. In the numerical results, the effects of the ramp-heating time parameter, the nonlocal parameter, and the power-law index on the considered physical quantities are presented and discussed in detail.展开更多
The propagation of single-event effects(SEEs)on a Xilinx Zynq-7000 system on chip(SoC)was inves-tigated using heavy-ion microbeam radiation.The irradia-tion results reveal several functional blocks’sensitivity locati...The propagation of single-event effects(SEEs)on a Xilinx Zynq-7000 system on chip(SoC)was inves-tigated using heavy-ion microbeam radiation.The irradia-tion results reveal several functional blocks’sensitivity locations and cross sections,for instance,the arithmetic logic unit,register,D-cache,and peripheral,while irradi-ating the on-chip memory(OCM)region.Moreover,event tree analysis was executed based on the obtained microbeam irradiation results.This study quantitatively assesses the probabilities of SEE propagation from the OCM to other blocks in the SoC.展开更多
文摘Phytochromes in seed plants are known to move into nuclei in a red light-dependent manner with or without interacting factors. Here, we show phytochrome relocation to the nuclear region in phytochrome-dependent Adiantum capillus-veneris spore germination by partial spore-irradiation experiments. The nuclear or non-nuclear region of imbibed spores was irradiated with a microbeam of red and/or far-red light and the localization of phytochrome involved in spore germination was estimated from the germination rate. The phytochrome for spore germination existed throughout whole spore under darkness after imbibition, but gradually migrated to the nuclear region following red light irradiation. In- tracellular distribution of PHY-GUS fusion proteins expressed in germinated spores by particle bombardment showed the migration of Acphy2, but not Acphyl, into nucleus in a red light-dependent manner, suggesting that Acphy2 is the photoreceptor for fern spore germination.
文摘We present a new analytical model for electrostatically actuated microbeams to explore the size effect by using the modified couple stress theory and the minimum total potential energy principle. A material length scale parameter is introduced to represent the size-dependent characteristics of microbeams. This model also accounts for the nonlinearities associated with the mid-plane stretching force and the electrostatical force. Numerical analysis for microbeams with clamped-clamped and cantilevered conditions has been performed. It is found that the intensity of size effect is closely associated with the thickness of the microbeam,and smaller beam thickness displays stronger size effect and hence yields smaller deffection and larger pull-in voltage. When the beam thickness is comparable to the material length scale parameter,the size effect is significant and the present theoretical model including the material length scale parameter is adequate for predicting the static behavior of microbeam-based MEMS.
基金supported by the National Key Research and Development Program of China(2018YFB0703500).
文摘The classical piezoelectric theory fails to capture the size-dependent electromechanical coupling behaviors of piezoelectric microstructures due to the lack of material length-scale parameters.This study presents the constitutive relations of a piezoelectric material in terms of irreducible transversely isotropic tensors that include material length-scale parameters.Using these relations and the general strain gradient theory,a size-dependent bending model is proposed for a bilayer cantilever microbeam consisting of a transversely isotropic piezoelectric layer and an isotropic elastic layer.Analytical solutions are provided for bilayer cantilever microbeams subjected to force load and voltage load.The proposed model can be simplified to the model incorporating only partial strain gradient effects.This study examines the effect of strain gradient by comparing the normalized electric potentials and deflections of different models.Numerical results show that the proposed model effectively captures size effects in piezoelectric microbeams,whereas simplified models underestimate size effects due to ignoring partial strain gradient effects.
文摘Using cotyledonary petioles as explants, the PAP cDNA controlled by wound-in-ducible promoter has been introduced into Brassica napus by coculture with Agrobacterium tumefaciens and laser microbeam puncture respectively. Regenerated plants resistant to gen-tamycin have been selected out. PCR amplification and Southern blotting analysis indicated that PAP cDNA together with wound-inducible promoter had been integrated into Brassica genome with transformation frequencies of 2.0% and 1.7% for two transformation methods respectively. The test of virus challenge showed that these transgenic Brassica plants were resistant in different degrees to mechanically inoculated TuMV.
基金Project supported by the National Key Research and Development Program of China(No.2017YFC0307604)the Talent Foundation of China University of Petroleum(No.Y1215042)the Graduate Innovation Program of China University of Petroleum(East China)(No.YCX2019084)
文摘The objective of this paper is to model the size-dependent thermo-mechanical behaviors of a shape memory polymer (SMP) microbeam.Size-dependent constitutive equations,which can capture the size effect of the SMP,are proposed based on the modified couple stress theory (MCST).The deformation energy expression of the SMP microbeam is obtained by employing the proposed size-dependent constitutive equation and Bernoulli-Euler beam theory.An SMP microbeam model,which includes the formulations of deflection,strain,curvature,stress and couple stress,is developed by using the principle of minimum potential energy and the separation of variables together.The sizedependent thermo-mechanical and shape memory behaviors of the SMP microbeam and the influence of the Poisson ratio are numerically investigated according to the developed SMP microbeam model.Results show that the size effects of the SMP microbeam are significant when the dimensionless height is small enough.However,they are too slight to be necessarily considered when the dimensionless height is large enough.The bending flexibility and stress level of the SMP microbeam rise with the increasing dimensionless height,while the couple stress level declines with the increasing dimensionless height.The larger the dimensionless height is,the more obvious the viscous property and shape memory effect of the SMP microbeam are.The Poisson ratio has obvious influence on the size-dependent behaviors of the SMP microbeam.The paper provides a theoretical basis and a quantitatively analyzing tool for the design and analysis of SMP micro-structures in the field of biological medicine,microelectronic devices and micro-electro-mechanical system (MEMS) self-assembling.
文摘On the basis of the Euler-Bernoulli hypothesis, nonlinear static and dynamic responses of a viscoelastic microbeam under two kinds of electric forces [a purely direct current （DC） and a combined current composed of a DC and an alternating current] are studied. By using Taylor series expansion, a governing equation of nonlinear integro-differential type is derived, and numerical analyses are performed. When a purely DC is applied, there exist an instantaneous pull-in voltage and a durable pull-in voltage of which the physical meanings are also given, whereas under an applied combined current, the effect of the element relaxation coefficient on the dynamic pull-in phenomenon is observed where the largest Lyapunov exponent is taken as a criterion for the dynamic pull-in instability of viscoelastic microbeams.
基金supported by the National Natural Science Foundation of China(Grant No.61274106)
文摘In this paper the single-event responses of the silicon germanium heterojunction bipolar transistors(SiGe HBTs) are investigated by TCAD simulations and laser microbeam experiment. A three-dimensional(3D) simulation model is established, the single event effect(SEE) simulation is further carried out on the basis of Si Ge HBT devices, and then, together with the laser microbeam test, the charge collection behaviors are analyzed, including the single event transient(SET) induced transient terminal currents, and the sensitive area of SEE charge collection. The simulations and experimental results are discussed in detail and it is demonstrated that the nature of the current transient is controlled by the behaviors of the collector–substrate(C/S) junction and charge collection by sensitive electrodes, thereby giving out the sensitive area and electrode of SiGe HBT in SEE.
文摘A method for microdissection, isolation and amplification of plant chromosomal fragments using laser microbeam and a glass microneedle was established. Firstly, 7H chromosome of barley (Hordeum vulgare L.) was dissected by Nd: YAG laserbeam with suitable parameters and the fragment comprising a satellite was isolated with a glass microneedle which was fixed on a micromanipulator. Then, the chromosomal fragment DNA was amplified by LA_PCR (linker adaptor PCR) for two rounds. The size of the DNA fragments of PCR products varied from 500-3 000 bp and the PCR products originated from the genome of barley were verified by Southern hybridization. Compared with previous reports, there are some advantages in this research. The performance is easier, the dissection is more precise and the cost is low. It also permits efficient amplification with only one single chromosome fragment. Laser microbeam_glass microneedle method may be useful in the microdissection of special chromosome regions, especially in plants with middle or small chromosomes.
基金Project supported by the National Natural Science Foundation of China (Grant No. 39470205)State Science and Technology Commision, and Science and Technolgy Commision of Anhui Province
文摘Laser tweezers can manipulate small particles, such as cells and organelles. When coupling them with laser microbeam selective fusion of two tobacco protoplasts containing some chloroplast was achieved. Physical and biological variables that affect laser trapping and laser-induced fusion were also discussed. The results show that the effect of chloroplast content and distribution on the yield of cell fusion is remarkable.
基金supported by the National Natural Science Foundation of China(Nos.1197283,U1632271)the National Key R&D Program of China(No.2021YFA1601400).
文摘High-energy proton microbeam facilities are powerful tools in space science,biology and cancer therapy studies.The primary limitations of the 50 MeV proton microbeam system are the poor beam quality provided by the cyclotron and the problem of intense scattering in the slit position.Here,we present an optical design for a cyclotron-based 50 MeV high-energy proton microbeam system with a micron-sized resolution.The microbeam system,which has an Oxford triplet lens configuration,has relatively small spherical aberrations and is insensitive to changes in the beam divergence angle and momentum spread.In addition,the energy filtration included in the system can reduce the beam momentum spread from 1 to 0.02%.The effects of lens parasitic aberrations and the lens fringe field on the beam spot resolution are also discussed.In addition,owing to the severe scattering of 50 MeV protons in slit materials,a slit system model based on the Geant4 toolkit enables the quantitative analysis of scattered protons and secondary particles.For the slit system settings under a 10-micron final beam spot,very few scattered protons can enter the quadrupole lens system and affect the focusing performance of the microbeam system,but the secondary radiation of neutrons and gamma rays generated at the collimation system should be considered for the 50 MeV proton microbeam.These data demonstrate that a 50 MeV proton microbeam system with a micron-sized beam spot based on a cyclotron is feasible.
文摘In this manuscript,we study a new version of the optical recursional binormal microbeam model for a flexible binormal microscale beam in terms of a binormal normalized operator.Also,we give new explanations for the optical recursional visco Landau-Lifshitz binormal electromagnetic binormal microscale beam.Finally,we obtain an optical application for the normalized visco Landau-Lifshitz electromagnetic binormal optimistic density with an optical binormal resonator.
基金Project supported by the National Natural Science Foundation of China (Nos. 11972176 and12062011)the Incubation Programme of Excellent Doctoral Dissertation-Lanzhou University of Technology。
文摘In extreme heat transfer environments, functionally graded materials(FGMs)have aroused great concern due to the excellent thermal shock resistance. With the development of micro-scale devices, the size-dependent effect has become an important issue. However, the classical continuum mechanical model fails on the micro-scale due to the influence of the size-dependent effect. Meanwhile, for thermoelastic behaviors limited to small-scale problems, Fourier's heat conduction law cannot explain the thermal wave effect. In order to capture the size-dependent effect and the thermal wave effect, the nonlocal generalized thermoelastic theory for the formulation of an FGM microbeam is adopted in the present work. For numerical validation, the transient responses for a simply supported FGM microbeam heated by the ramp-type heating are considered.The governing equations are formulated and solved by employing the Laplace transform techniques. In the numerical results, the effects of the ramp-heating time parameter, the nonlocal parameter, and the power-law index on the considered physical quantities are presented and discussed in detail.
基金This work was supported by the National Natural Science Foundation of China(Nos.11575138,11835006,11690040,11690043,and 11705216)the Innovation Center of Radiation Application(No.KFZC2019050321)the China Scholarships Council program(No.201906280343).
文摘The propagation of single-event effects(SEEs)on a Xilinx Zynq-7000 system on chip(SoC)was inves-tigated using heavy-ion microbeam radiation.The irradia-tion results reveal several functional blocks’sensitivity locations and cross sections,for instance,the arithmetic logic unit,register,D-cache,and peripheral,while irradi-ating the on-chip memory(OCM)region.Moreover,event tree analysis was executed based on the obtained microbeam irradiation results.This study quantitatively assesses the probabilities of SEE propagation from the OCM to other blocks in the SoC.
