MicroRNAs (miRNAs) are a specialized class of small silencing RNAs that regulate gene expression in eukaryotes.In plants,miRNAs negatively regulate target mRNAs containing a highly complementary sequence by either mRN...MicroRNAs (miRNAs) are a specialized class of small silencing RNAs that regulate gene expression in eukaryotes.In plants,miRNAs negatively regulate target mRNAs containing a highly complementary sequence by either mRNA cleavage or translational repression.As a model plant to study fleshy fruit ripening,miRNA studies in tomato have made great progress recently.MiRNAs were predicted to be involved in nearly all biological processes in tomato,particularly development,differentiation,and biotic and abiotic stress responses.Surprisingly,several miRNAs were verified to be involved in tomato fruit ripening and senescence.Recent studies suggest that miRNAs are related to host-virus interactions,which raises the possibility that miRNAs can be used as diagnostic markers for response to virus infection in tomato plants.In this review,we summarize our current knowledge systematically and advance future directions for miRNA research in tomato.展开更多
Technical challenges have always been part of underground mining activities,however,some of these challenges grow in complexity as mining occurs in deeper and deeper settings.One such challenge is rock mass stability ...Technical challenges have always been part of underground mining activities,however,some of these challenges grow in complexity as mining occurs in deeper and deeper settings.One such challenge is rock mass stability and the risk of rockburst events.To overcome these challenges,and to limit the risks and impacts of events such as rockbursts,advanced solutions must be developed and best practices implemented.Rockbursts are common in underground mines and substantially threaten the safety of personnel and equipment,and can cause major disruptions in mine development and operations.Rockbursts consist of violent wall rock failures associated with high energy rock projections in response to the instantaneous stress release in rock mass under high strain conditions.Therefore,it is necessary to develop a good understanding of the conditions and mechanisms leading to a rockburst,and to improve risk assessment methods.The capacity to properly estimate the risks of rockburst occurrence is essential in underground operations.However,a limited number of studies have examined and compared yet different empirical methods of rockburst.The current understanding of this important hazard in the mining industry is summarized in this paper to provide the necessary perspective or tools to best assess the risks of rockburst occurrence in deep mines.The various classifications of rockbursts and their mechanisms are discussed.The paper also reviews the current empirical methods of rockburst prediction,which are mostly dependent on geomechanical parameters of the rock such as uniaxial compressive strength of the rock,as well as its tensile strength and elasticity modulus.At the end of this paper,some current achievements and limitations of empirical methods are discussed.展开更多
Functional characterization of every single protein is a major challenge of the post-genomic era. The large-scale analysis of a cell's proteins, proteomics, seeks to provide these proteins with reliable annotation...Functional characterization of every single protein is a major challenge of the post-genomic era. The large-scale analysis of a cell's proteins, proteomics, seeks to provide these proteins with reliable annotations regarding their interaction partners and functions in the cellular machinery. An important step on this way is to determine the subcellular localization of each protein. Eukaryotic cells are divided into subcellular compartments, or organelles. Transport across the membrane into the organelles is a highly regulated and complex cellular process. Predicting the subcellular localization by computational means has been an area of vivid activity during recent years. The publicly available prediction methods differ mainly in four aspects: the underlying biological motivation, the computational method used, localization coverage, and reliability, which are of importance to the user. This review provides a short description of the main events in the protein sorting process and an overview of the most commonly used methods in this field.展开更多
基金supported by the National Natural Science Foundation of China (Grant Nos. 30871741 and 30972037)
文摘MicroRNAs (miRNAs) are a specialized class of small silencing RNAs that regulate gene expression in eukaryotes.In plants,miRNAs negatively regulate target mRNAs containing a highly complementary sequence by either mRNA cleavage or translational repression.As a model plant to study fleshy fruit ripening,miRNA studies in tomato have made great progress recently.MiRNAs were predicted to be involved in nearly all biological processes in tomato,particularly development,differentiation,and biotic and abiotic stress responses.Surprisingly,several miRNAs were verified to be involved in tomato fruit ripening and senescence.Recent studies suggest that miRNAs are related to host-virus interactions,which raises the possibility that miRNAs can be used as diagnostic markers for response to virus infection in tomato plants.In this review,we summarize our current knowledge systematically and advance future directions for miRNA research in tomato.
基金the funding received by a grant from Natural Sciences and Engineering Research of Canada(NSERC)for this study.
文摘Technical challenges have always been part of underground mining activities,however,some of these challenges grow in complexity as mining occurs in deeper and deeper settings.One such challenge is rock mass stability and the risk of rockburst events.To overcome these challenges,and to limit the risks and impacts of events such as rockbursts,advanced solutions must be developed and best practices implemented.Rockbursts are common in underground mines and substantially threaten the safety of personnel and equipment,and can cause major disruptions in mine development and operations.Rockbursts consist of violent wall rock failures associated with high energy rock projections in response to the instantaneous stress release in rock mass under high strain conditions.Therefore,it is necessary to develop a good understanding of the conditions and mechanisms leading to a rockburst,and to improve risk assessment methods.The capacity to properly estimate the risks of rockburst occurrence is essential in underground operations.However,a limited number of studies have examined and compared yet different empirical methods of rockburst.The current understanding of this important hazard in the mining industry is summarized in this paper to provide the necessary perspective or tools to best assess the risks of rockburst occurrence in deep mines.The various classifications of rockbursts and their mechanisms are discussed.The paper also reviews the current empirical methods of rockburst prediction,which are mostly dependent on geomechanical parameters of the rock such as uniaxial compressive strength of the rock,as well as its tensile strength and elasticity modulus.At the end of this paper,some current achievements and limitations of empirical methods are discussed.
文摘Functional characterization of every single protein is a major challenge of the post-genomic era. The large-scale analysis of a cell's proteins, proteomics, seeks to provide these proteins with reliable annotations regarding their interaction partners and functions in the cellular machinery. An important step on this way is to determine the subcellular localization of each protein. Eukaryotic cells are divided into subcellular compartments, or organelles. Transport across the membrane into the organelles is a highly regulated and complex cellular process. Predicting the subcellular localization by computational means has been an area of vivid activity during recent years. The publicly available prediction methods differ mainly in four aspects: the underlying biological motivation, the computational method used, localization coverage, and reliability, which are of importance to the user. This review provides a short description of the main events in the protein sorting process and an overview of the most commonly used methods in this field.
