Mesenchymal stem cells （MSCs） show the great promise for the treatment of a variety of diseases because of their self-renewal and multipotential abilities. MSCs are generally cultured on two-dimensional （2D） subst...Mesenchymal stem cells （MSCs） show the great promise for the treatment of a variety of diseases because of their self-renewal and multipotential abilities. MSCs are generally cultured on two-dimensional （2D） substrate in vitro. There are indications that they may simultaneously lose their sternness and multipotentiality as the result of prolonged 2D culture. In this study, we used three-dimensional （3D） collagen scaffolds as rat MSCs cartier and compared the properties of MSCs on 3D collagen scaffolds with monolayer cultured MSCs. The results demonstrated that collagen scaffolds were suitable for rat MSCs adherence and proliferation. More importantly, compared to MSCs under 2D culture, 3D MSCs significantly maintained higher expression levels of stemness genes （Oct4, Sox2, Rex-1 and Nanog）, yielded high frequencies of colony-forming units-fibroblastic （CFU-F） and showed enhanced osteogenic and adipogenic differentiation efficiency upon induction. Thus, 3D collagen scaffolds may be beneficial for expanding rat MSCs while maintaining the stem cell properties in vitro.展开更多
目的:研究骨髓间质干细胞(M esenchym al stem cells,MSCs)下调免疫反应的作用及其机制。方法:采用流式细胞分析(FCM)鉴定MSCs的纯度,用3H-TdR掺入法测定MSCs对ConA刺激的淋巴细胞增殖的影响,利用RT-PCR和Real-tim e PCR检测TGF-β1、I...目的:研究骨髓间质干细胞(M esenchym al stem cells,MSCs)下调免疫反应的作用及其机制。方法:采用流式细胞分析(FCM)鉴定MSCs的纯度,用3H-TdR掺入法测定MSCs对ConA刺激的淋巴细胞增殖的影响,利用RT-PCR和Real-tim e PCR检测TGF-β1、IL-8、IL-10的基因表达,同时进行皮肤移植模型研究。结果:MSCs对ConA刺激的淋巴细胞增殖的影响与MSCs和淋巴细胞的比值有关。RT-PCR结果显示MSCs对淋巴细胞分泌TGF-β1无显著性影响,而Real-tim e PCR的结果显示MSCs促进淋巴细胞分泌IL-10,减少淋巴细胞分泌IL-8。皮肤移植实验显示MSCs能延长异种移植皮肤的存活时间。结论:MSCs能下调免疫反应和抑制皮肤移植排斥反应,其可能的机制是通过促进淋巴细胞分泌IL-10以及减少淋巴细胞分泌IL-8来发挥作用。展开更多
基金supported by the National Natural Science Foundation of China(81273597,81172986,81302801)Innovation and Nursery Foundation of Chinese PLA General Hospital(11KMM24,13KMM18)Clinical Study Supporting Foundation(2012FC-TSYS-4010)
基金supported by the grants from the Ministry of Science and Technology of China(Nos.2011CB965001 and 2011CB710905)the Knowledge Innovation Program of the Chinese Academy of Sciences(Nos.KSCX2-YW-R-232, KJCX2-YW-L08 and KYQY-QN-015)
文摘Mesenchymal stem cells （MSCs） show the great promise for the treatment of a variety of diseases because of their self-renewal and multipotential abilities. MSCs are generally cultured on two-dimensional （2D） substrate in vitro. There are indications that they may simultaneously lose their sternness and multipotentiality as the result of prolonged 2D culture. In this study, we used three-dimensional （3D） collagen scaffolds as rat MSCs cartier and compared the properties of MSCs on 3D collagen scaffolds with monolayer cultured MSCs. The results demonstrated that collagen scaffolds were suitable for rat MSCs adherence and proliferation. More importantly, compared to MSCs under 2D culture, 3D MSCs significantly maintained higher expression levels of stemness genes （Oct4, Sox2, Rex-1 and Nanog）, yielded high frequencies of colony-forming units-fibroblastic （CFU-F） and showed enhanced osteogenic and adipogenic differentiation efficiency upon induction. Thus, 3D collagen scaffolds may be beneficial for expanding rat MSCs while maintaining the stem cell properties in vitro.
文摘目的:研究骨髓间质干细胞(M esenchym al stem cells,MSCs)下调免疫反应的作用及其机制。方法:采用流式细胞分析(FCM)鉴定MSCs的纯度,用3H-TdR掺入法测定MSCs对ConA刺激的淋巴细胞增殖的影响,利用RT-PCR和Real-tim e PCR检测TGF-β1、IL-8、IL-10的基因表达,同时进行皮肤移植模型研究。结果:MSCs对ConA刺激的淋巴细胞增殖的影响与MSCs和淋巴细胞的比值有关。RT-PCR结果显示MSCs对淋巴细胞分泌TGF-β1无显著性影响,而Real-tim e PCR的结果显示MSCs促进淋巴细胞分泌IL-10,减少淋巴细胞分泌IL-8。皮肤移植实验显示MSCs能延长异种移植皮肤的存活时间。结论:MSCs能下调免疫反应和抑制皮肤移植排斥反应,其可能的机制是通过促进淋巴细胞分泌IL-10以及减少淋巴细胞分泌IL-8来发挥作用。
