This study was aimed to evaluate the effects of dietary n-6：n-3 ratio and Vitamin E on the membrane properties and motility characteristics of spermatozoa in boars. Forty Duroc boars were randomly distributed in a 2 ...This study was aimed to evaluate the effects of dietary n-6：n-3 ratio and Vitamin E on the membrane properties and motility characteristics of spermatozoa in boars. Forty Duroc boars were randomly distributed in a 2 × 2 factorial design with two n-6：n-3 ratios （14.4 and 6.6） and two Vitamin E levels （200 and 400 mg kg-1）. During 16 weeks of treatment, fresh semen was collected at weeks O, 8, 12, and 16 for measurements of motility characteristics, contents of fatty acids, membrane properties （membrane fluidity and membrane integrity）, and lipid peroxidation of the spermatozoa. The semen was diluted in Beltsville Thawing Solution （BTS） extender and stored at 17℃, and the sperm motility was assessed at 12, 36, 72, and 120 h of storage. The 6.6 n-6.n-3 dietary ratio increased the contents of n-3 polyunsaturated fatty acids （PUFAs） and docosabexaenoic acid （DHA） and improved the membrane integrity and membrane fluidity of the spermatozoa, resulting in notably increased total motility, sperm progressive motility, and velocity parameters of fresh semen. Feeding diet with Vitamin E （400 mg kg-1） prevented sperm lipid peroxidation, and resulted in higher total motility and sperm progressive motility in fresh and liquid stored semen. In conclusion, the adjustment of n-6.n-3 ratio （6.6） and supply of Vitamin E （400 mg kg-1） successfully improved sperm motility characteristics and thus may be beneficial to the fertility of boars, which might be due to the modification of the physical and functional properties of spermatozoa membrane in response to dietary supplementation.展开更多
目的研究出生后不同发育阶段Wistar大鼠视网膜神经节细胞(RGCs)的膜学特性,包括被动膜学特性和去极化脉冲诱发的动作电位(Action potential,AP),并分析其与年龄的关系.方法应用视网膜切片膜片钳全细胞记录技术,获得7～30 d Wistar大鼠...目的研究出生后不同发育阶段Wistar大鼠视网膜神经节细胞(RGCs)的膜学特性,包括被动膜学特性和去极化脉冲诱发的动作电位(Action potential,AP),并分析其与年龄的关系.方法应用视网膜切片膜片钳全细胞记录技术,获得7～30 d Wistar大鼠视网膜神经节细胞的膜片钳全细胞记录.结果①记录了112个视网膜神经节细胞;②随着发育,RGCs的膜学特性发生显著变化,兴奋性增加,睁眼前组与睁眼后组间有显著性差异;③去极化脉冲诱发其产生的动作电位有3种形式:单峰型、瞬变型、持续型.随着视觉发育,单峰型逐渐减少,持续型逐渐增加,细胞成熟时未见到单峰型,只见到瞬变型和持续型.随着发育,RGCs的电生理特性发生显著变化.结论①睁眼后形觉刺激促进RGCs膜学特性的成熟;②随着发育,RGCs的AP类型发生显著变化,单峰型是RGCs发育过程中的一种前期状态,最终分化为瞬变型和持续型RGCs.展开更多
文摘This study was aimed to evaluate the effects of dietary n-6：n-3 ratio and Vitamin E on the membrane properties and motility characteristics of spermatozoa in boars. Forty Duroc boars were randomly distributed in a 2 × 2 factorial design with two n-6：n-3 ratios （14.4 and 6.6） and two Vitamin E levels （200 and 400 mg kg-1）. During 16 weeks of treatment, fresh semen was collected at weeks O, 8, 12, and 16 for measurements of motility characteristics, contents of fatty acids, membrane properties （membrane fluidity and membrane integrity）, and lipid peroxidation of the spermatozoa. The semen was diluted in Beltsville Thawing Solution （BTS） extender and stored at 17℃, and the sperm motility was assessed at 12, 36, 72, and 120 h of storage. The 6.6 n-6.n-3 dietary ratio increased the contents of n-3 polyunsaturated fatty acids （PUFAs） and docosabexaenoic acid （DHA） and improved the membrane integrity and membrane fluidity of the spermatozoa, resulting in notably increased total motility, sperm progressive motility, and velocity parameters of fresh semen. Feeding diet with Vitamin E （400 mg kg-1） prevented sperm lipid peroxidation, and resulted in higher total motility and sperm progressive motility in fresh and liquid stored semen. In conclusion, the adjustment of n-6.n-3 ratio （6.6） and supply of Vitamin E （400 mg kg-1） successfully improved sperm motility characteristics and thus may be beneficial to the fertility of boars, which might be due to the modification of the physical and functional properties of spermatozoa membrane in response to dietary supplementation.
文摘目的研究出生后不同发育阶段Wistar大鼠视网膜神经节细胞(RGCs)的膜学特性,包括被动膜学特性和去极化脉冲诱发的动作电位(Action potential,AP),并分析其与年龄的关系.方法应用视网膜切片膜片钳全细胞记录技术,获得7～30 d Wistar大鼠视网膜神经节细胞的膜片钳全细胞记录.结果①记录了112个视网膜神经节细胞;②随着发育,RGCs的膜学特性发生显著变化,兴奋性增加,睁眼前组与睁眼后组间有显著性差异;③去极化脉冲诱发其产生的动作电位有3种形式:单峰型、瞬变型、持续型.随着视觉发育,单峰型逐渐减少,持续型逐渐增加,细胞成熟时未见到单峰型,只见到瞬变型和持续型.随着发育,RGCs的电生理特性发生显著变化.结论①睁眼后形觉刺激促进RGCs膜学特性的成熟;②随着发育,RGCs的AP类型发生显著变化,单峰型是RGCs发育过程中的一种前期状态,最终分化为瞬变型和持续型RGCs.
