Background Recent studies have shown that T helper type-2 （Th2） cells can induce the apoptosis of CD4＋CD25＋ Treg cells or resist the immunosuppressive effect of Treg cells.We hypothesize that an imbalance of Th2/T...Background Recent studies have shown that T helper type-2 （Th2） cells can induce the apoptosis of CD4＋CD25＋ Treg cells or resist the immunosuppressive effect of Treg cells.We hypothesize that an imbalance of Th2/Treg is present in patients with allergic asthma.Methods Twenty-two patients with mild asthma,17 patients with moderate to severe asthma,and 20 healthy donors were enrolled.All patients were allergic to house dust mites.The proportion of peripheral blood CD4＋CD25＋ Treg cells and Th2 cells were determined by flow cytometry.The concentration of interleukin （IL）-10,transforming growth factor （TGF）-β and IL-4 in plasma was determined by enzyme linked immunosorbent assay.In these subjects,peripheral blood mononuclear cells from 17 mild asthmatic patients,13 moderate to severe asthmatic patients and 14 healthy donors were acquired and expression of forkhead box P3 （Foxp3） and GATA-3 mRNA was detected by reverse-transcriptase polymerase chain reaction.Results Compared with healthy donors and patients with mild asthma,the percent of CD4＋CD25＋ Treg cells and plasma IL-10 levels were decreased in patients with moderate to severe asthma.There were no significant differences in Foxp3 mRNA expression among three groups,but a downward trend seen among patients with asthma.However,the percent of Th2 cells,IL-4 levels and expression of GATA-3 mRNA was markedly higher in patients with mild and moderate to severe asthma than in the control group.The ratio of Th2/Treg and their cytokines was increased in allergic asthma,especially for moderate to severe asthma.The ratio of GATA-3/Foxp3 mRNA was also increased in allergic asthma.In patients with moderate to severe asthma,the percentage of peripheral blood Treg cells was negatively correlated to the percentage of Th2 cells and IL-4 levels.Conclusions The decline of CD4＋CD25＋ Treg cells in patients with moderate to severe asthma may play an important role in progress of the disease.Furthermore,the deficiency of CD4＋CD25＋ Treg cells was asso展开更多
Objective The aim of this study was to investigate the prognostic relevance of acid phosphatase 1(ACP1) expression in myeloma patients by using Gene Expression Omnibus(GEO) datasets.Methods A comprehensive search was ...Objective The aim of this study was to investigate the prognostic relevance of acid phosphatase 1(ACP1) expression in myeloma patients by using Gene Expression Omnibus(GEO) datasets.Methods A comprehensive search was performed in the GEO database in order to find appropriate datasets. The expression level of ACP1 was extracted from the dataset involving both newly diagnosed and relapsed myeloma patients, and a comparison was made. Clinical follow-up data and ACP1 expression were extracted, and survival analysis of overall survival was performed to compare the high-(top quartile) and low-expression(bottom quartile) groups. Analyses using Kaplan-Meier estimation, log-rank test, and restricted mean survival time(RMST) comparison were performed.Results The GSE 6477 dataset was used to make a comparison of the ACP1 expression levels among patients with newly diagnosed and relapsed myeloma. The ACP1 expression level was significantly higher in the relapsed group than in the newly diagnosed group [mean difference =-262.9, 95% confidence interval(CI) =(-420.2,-105.5), P = 0.002]. The GSE 2658 dataset was used for investigating the prognostic relevance of ACP1 expression in myeloma. The ACP1 high-expression group had a significantly worse prognosis [low vs high: hazard ratio = 0.54, 95% CI =(0.31, 0.95); χ2 = 5.02, log rank P = 0.0314]. The median survival was 55.9 months in the high-expression group and was not reached in the low-expression group. The restricted mean time loss(95% CI) was 11.03(12.97, 23.11) and 18.04(12.97, 23.11) for the low-and high-expression groups, respectively. The ratio of RMST(95% CI) between the two groups(high vs low) was 0.87(0.77, 0.99; P = 0.03).Conclusion Our study, for the first time, showed that ACP1 predicts the prognosis in multiple myeloma patients. Further studies are needed to determine the potential mechanism by which ACP1 is associated with clinical outcomes and should focus on the differential roles of low-molecular-weight protein tyrosine phosphatase(LMWPTP) isoforms.展开更多
文摘Background Recent studies have shown that T helper type-2 （Th2） cells can induce the apoptosis of CD4＋CD25＋ Treg cells or resist the immunosuppressive effect of Treg cells.We hypothesize that an imbalance of Th2/Treg is present in patients with allergic asthma.Methods Twenty-two patients with mild asthma,17 patients with moderate to severe asthma,and 20 healthy donors were enrolled.All patients were allergic to house dust mites.The proportion of peripheral blood CD4＋CD25＋ Treg cells and Th2 cells were determined by flow cytometry.The concentration of interleukin （IL）-10,transforming growth factor （TGF）-β and IL-4 in plasma was determined by enzyme linked immunosorbent assay.In these subjects,peripheral blood mononuclear cells from 17 mild asthmatic patients,13 moderate to severe asthmatic patients and 14 healthy donors were acquired and expression of forkhead box P3 （Foxp3） and GATA-3 mRNA was detected by reverse-transcriptase polymerase chain reaction.Results Compared with healthy donors and patients with mild asthma,the percent of CD4＋CD25＋ Treg cells and plasma IL-10 levels were decreased in patients with moderate to severe asthma.There were no significant differences in Foxp3 mRNA expression among three groups,but a downward trend seen among patients with asthma.However,the percent of Th2 cells,IL-4 levels and expression of GATA-3 mRNA was markedly higher in patients with mild and moderate to severe asthma than in the control group.The ratio of Th2/Treg and their cytokines was increased in allergic asthma,especially for moderate to severe asthma.The ratio of GATA-3/Foxp3 mRNA was also increased in allergic asthma.In patients with moderate to severe asthma,the percentage of peripheral blood Treg cells was negatively correlated to the percentage of Th2 cells and IL-4 levels.Conclusions The decline of CD4＋CD25＋ Treg cells in patients with moderate to severe asthma may play an important role in progress of the disease.Furthermore,the deficiency of CD4＋CD25＋ Treg cells was asso
文摘Objective The aim of this study was to investigate the prognostic relevance of acid phosphatase 1(ACP1) expression in myeloma patients by using Gene Expression Omnibus(GEO) datasets.Methods A comprehensive search was performed in the GEO database in order to find appropriate datasets. The expression level of ACP1 was extracted from the dataset involving both newly diagnosed and relapsed myeloma patients, and a comparison was made. Clinical follow-up data and ACP1 expression were extracted, and survival analysis of overall survival was performed to compare the high-(top quartile) and low-expression(bottom quartile) groups. Analyses using Kaplan-Meier estimation, log-rank test, and restricted mean survival time(RMST) comparison were performed.Results The GSE 6477 dataset was used to make a comparison of the ACP1 expression levels among patients with newly diagnosed and relapsed myeloma. The ACP1 expression level was significantly higher in the relapsed group than in the newly diagnosed group [mean difference =-262.9, 95% confidence interval(CI) =(-420.2,-105.5), P = 0.002]. The GSE 2658 dataset was used for investigating the prognostic relevance of ACP1 expression in myeloma. The ACP1 high-expression group had a significantly worse prognosis [low vs high: hazard ratio = 0.54, 95% CI =(0.31, 0.95); χ2 = 5.02, log rank P = 0.0314]. The median survival was 55.9 months in the high-expression group and was not reached in the low-expression group. The restricted mean time loss(95% CI) was 11.03(12.97, 23.11) and 18.04(12.97, 23.11) for the low-and high-expression groups, respectively. The ratio of RMST(95% CI) between the two groups(high vs low) was 0.87(0.77, 0.99; P = 0.03).Conclusion Our study, for the first time, showed that ACP1 predicts the prognosis in multiple myeloma patients. Further studies are needed to determine the potential mechanism by which ACP1 is associated with clinical outcomes and should focus on the differential roles of low-molecular-weight protein tyrosine phosphatase(LMWPTP) isoforms.
