The olive species(Olea europaea L.)is an ancient traditional crop grown under rainfed conditions in the Mediterranean Basin.In response to the growing national and international demand for olive oil,the olive cultivar...The olive species(Olea europaea L.)is an ancient traditional crop grown under rainfed conditions in the Mediterranean Basin.In response to the growing national and international demand for olive oil,the olive cultivars are introduced into highly arid new bioclimatic areas.Subsequently,the morpho-physiology and phytochemistry of olive trees are potentially changing among cultivar types and geographical conditions.In the present work,we have undertaken an assessment on the impacts of geographical location and cultivar types on the leaf morpho-physiology and phytochemistry of olive trees.Thus,leaves of the two most cultivated olive tree varieties,Chemlal and Sigoise,were collected from three geographical regions(Setif,Batna,and Eloued)with increasing aridity in Algeria.Leaf samples from the geographical regions were analyzed using the standard physiological experiment,colorimetric method,and a chromatography assay.Leaves of both cultivars exhibited a significant variance in terms of the leaf shape index but not for the leaf tissue density,specific leaf weight,and specific leaf area.Photosynthetic pigment contents were affected by both cultivar type and geographical location,with the lowest pigment content recorded in the Sigoise cultivar from the Setif region.Compared with the Setif and Batna regions,dried leaves of both cultivars from the Eloued region showed the higher levels of the total polyphenol,total flavonoid,and total tannin,as well as a better antioxidant capacity.Liquid chromatography-mass spectrometry analysis of all leaf extracts identified the following phenolic acids as major compounds:oleuropein,naringin,apigenin-7-O-glucoside,kaempferol,quercetin,quercitrin,luteolin-7-O-naringenin,and quinic acid.Lower contents were found for p-Coumaric acid,trans-Ferulic acid,hyperoside,rutin,apigenin,caffeic acid,protocatechuic acid,o-Coumaric acid,and gallic acid.Also,epicatechin and catechin+were not found in the leaf extracts of the Sigoise cultivar.The leaf organic extracts in both cultivars displayed promisin展开更多
以玉米高抗灰斑病自交系齐319与高感病自交系Ye478构建的RILS(重组自交系)为试材,通过两年田间表型鉴定,选取极端表型家系高抗16个,高感15个,利用SSR分子标记,并结合群体分离分析方法(BSA)筛选玉米抗灰斑病连锁标记并进行基因定位。结...以玉米高抗灰斑病自交系齐319与高感病自交系Ye478构建的RILS(重组自交系)为试材,通过两年田间表型鉴定,选取极端表型家系高抗16个,高感15个,利用SSR分子标记,并结合群体分离分析方法(BSA)筛选玉米抗灰斑病连锁标记并进行基因定位。结果表明,在玉米第1连锁群上检测到1个主效抗病基因位点(QTL),与两侧的分子标记umc2614和bnlg1803遗传图距分别为4.74 c M和3.78 c M,该抗病基因位点可解释40.9%的表型变异率,抗病基因来源于齐319,加性效应达到了-7.817 5。展开更多
文摘The olive species(Olea europaea L.)is an ancient traditional crop grown under rainfed conditions in the Mediterranean Basin.In response to the growing national and international demand for olive oil,the olive cultivars are introduced into highly arid new bioclimatic areas.Subsequently,the morpho-physiology and phytochemistry of olive trees are potentially changing among cultivar types and geographical conditions.In the present work,we have undertaken an assessment on the impacts of geographical location and cultivar types on the leaf morpho-physiology and phytochemistry of olive trees.Thus,leaves of the two most cultivated olive tree varieties,Chemlal and Sigoise,were collected from three geographical regions(Setif,Batna,and Eloued)with increasing aridity in Algeria.Leaf samples from the geographical regions were analyzed using the standard physiological experiment,colorimetric method,and a chromatography assay.Leaves of both cultivars exhibited a significant variance in terms of the leaf shape index but not for the leaf tissue density,specific leaf weight,and specific leaf area.Photosynthetic pigment contents were affected by both cultivar type and geographical location,with the lowest pigment content recorded in the Sigoise cultivar from the Setif region.Compared with the Setif and Batna regions,dried leaves of both cultivars from the Eloued region showed the higher levels of the total polyphenol,total flavonoid,and total tannin,as well as a better antioxidant capacity.Liquid chromatography-mass spectrometry analysis of all leaf extracts identified the following phenolic acids as major compounds:oleuropein,naringin,apigenin-7-O-glucoside,kaempferol,quercetin,quercitrin,luteolin-7-O-naringenin,and quinic acid.Lower contents were found for p-Coumaric acid,trans-Ferulic acid,hyperoside,rutin,apigenin,caffeic acid,protocatechuic acid,o-Coumaric acid,and gallic acid.Also,epicatechin and catechin+were not found in the leaf extracts of the Sigoise cultivar.The leaf organic extracts in both cultivars displayed promisin
文摘以玉米高抗灰斑病自交系齐319与高感病自交系Ye478构建的RILS(重组自交系)为试材,通过两年田间表型鉴定,选取极端表型家系高抗16个,高感15个,利用SSR分子标记,并结合群体分离分析方法(BSA)筛选玉米抗灰斑病连锁标记并进行基因定位。结果表明,在玉米第1连锁群上检测到1个主效抗病基因位点(QTL),与两侧的分子标记umc2614和bnlg1803遗传图距分别为4.74 c M和3.78 c M,该抗病基因位点可解释40.9%的表型变异率,抗病基因来源于齐319,加性效应达到了-7.817 5。
