The lateral flow immuno assay (LFIA) has emerged as a powerful tool for rapid scree ning owing to its simplicity and flexibility for detection of various biomarkers. However, conventional LFIA strips have several disa...The lateral flow immuno assay (LFIA) has emerged as a powerful tool for rapid scree ning owing to its simplicity and flexibility for detection of various biomarkers. However, conventional LFIA strips have several disadvantages, including limits in quantitative analysis and low sensitivity. Here we developed a novel surface-e nhanced Rama n scatteri ng LFIA based on non spherical gap-e nhanced Raman tags (GERTs), with Raman molecules (RMs) embedded in a 1-nm gap between Au nanorod core and Au shell. Such tags have a strong and uniform surface-enhanced Rama n scattering (SERS) resp on se, an order of mag nitude higher than that of other comm on SERS tags such as Au nano rods, nano stars, Au nano shells with surface-adsorbed RMs, or spherical GERTs with embedded RMs. The feasibility of the tags was dem on strated by the semiqua ntitative and sen sitive detecti on of the heart disease biomarker cardiac tropo nin I (cTnI). GERTs were conjugated with mono clonal antibodies and used for LFIA in the same way as ordinary functionalized colloidal gold. The presenee of the target antigen, cTnI, was identified by Raman microscopy mapping of the test zone. With the SERS-based LFIA, the limit of cTnI detection was about 0.1 ng/mL. This value is within the diagnostic range of cTnI in the blood serum of patients with heart infarction and is 30 times lower than that of the colorimetric LFIA test using the same antibodies and either GERTs or colloidal gold as labels.展开更多
目的:通过对神经侧支发芽过程中外源性神经生长因子对生长相关蛋白-43(gr owt h associ at ed pr ot ei n-43,GAP-43)的调控,探讨神经侧支发芽再生的机制。方法:SD大鼠共40只,建立胫腓神经端侧吻合模型,随机分为神经生长因子(nervegr ow...目的:通过对神经侧支发芽过程中外源性神经生长因子对生长相关蛋白-43(gr owt h associ at ed pr ot ei n-43,GAP-43)的调控,探讨神经侧支发芽再生的机制。方法:SD大鼠共40只,建立胫腓神经端侧吻合模型,随机分为神经生长因子(nervegr owt h f act or,NGF)组和生理盐水(SAL)组,各组又分为1周、2周、4周和8周组。切取吻合近端和远端神经纵切片标本,进行GAP-43的免疫组织化学反应,并结合计算机图像分析进行反应强度的半定量分析。结果:NGF组GAP-43表达显著增高。结论:应用NGF可以促进端侧吻合后神经侧支发芽。展开更多
Surgical accesses to lesions of the posterolateral pontomesencephalic junction（PMJ）region and the posterolateral tentorial gap remain a challenge in the field of neurosurgery.Since the first report of application of...Surgical accesses to lesions of the posterolateral pontomesencephalic junction（PMJ）region and the posterolateral tentorial gap remain a challenge in the field of neurosurgery.Since the first report of application of the extreme lateral supracerebellar infratentorial（ELSI）approach in resecting the PMJ lesions in 2000,a few articles concerning the ELSI approach have been published.The present review mainly provided an intimate introduction of the ELSI approach,and evalu- ated it in facets of patient position,skin incision,craniectomy,draining veins,retraction against the cerebellum,exposure limits,patient healing,as well as advantages and limitations compared with other approaches.The ELSI approach is proposed to be a very young and promising approach to access the lesions of posterolateral PMJ region and the posterolateral tentorial gap.Besides,it has several advantages such as having a shorter surgical pathway,causing less surgical complications,laborsaving,etc.Still,more studies are needed to improve this approach.展开更多
文摘The lateral flow immuno assay (LFIA) has emerged as a powerful tool for rapid scree ning owing to its simplicity and flexibility for detection of various biomarkers. However, conventional LFIA strips have several disadvantages, including limits in quantitative analysis and low sensitivity. Here we developed a novel surface-e nhanced Rama n scatteri ng LFIA based on non spherical gap-e nhanced Raman tags (GERTs), with Raman molecules (RMs) embedded in a 1-nm gap between Au nanorod core and Au shell. Such tags have a strong and uniform surface-enhanced Rama n scattering (SERS) resp on se, an order of mag nitude higher than that of other comm on SERS tags such as Au nano rods, nano stars, Au nano shells with surface-adsorbed RMs, or spherical GERTs with embedded RMs. The feasibility of the tags was dem on strated by the semiqua ntitative and sen sitive detecti on of the heart disease biomarker cardiac tropo nin I (cTnI). GERTs were conjugated with mono clonal antibodies and used for LFIA in the same way as ordinary functionalized colloidal gold. The presenee of the target antigen, cTnI, was identified by Raman microscopy mapping of the test zone. With the SERS-based LFIA, the limit of cTnI detection was about 0.1 ng/mL. This value is within the diagnostic range of cTnI in the blood serum of patients with heart infarction and is 30 times lower than that of the colorimetric LFIA test using the same antibodies and either GERTs or colloidal gold as labels.
文摘目的:通过对神经侧支发芽过程中外源性神经生长因子对生长相关蛋白-43(gr owt h associ at ed pr ot ei n-43,GAP-43)的调控,探讨神经侧支发芽再生的机制。方法:SD大鼠共40只,建立胫腓神经端侧吻合模型,随机分为神经生长因子(nervegr owt h f act or,NGF)组和生理盐水(SAL)组,各组又分为1周、2周、4周和8周组。切取吻合近端和远端神经纵切片标本,进行GAP-43的免疫组织化学反应,并结合计算机图像分析进行反应强度的半定量分析。结果:NGF组GAP-43表达显著增高。结论:应用NGF可以促进端侧吻合后神经侧支发芽。
基金supported by the grants from Natural Science Foundation of Shandong Province(No.Z2008C12,Y2006C13)
文摘Surgical accesses to lesions of the posterolateral pontomesencephalic junction（PMJ）region and the posterolateral tentorial gap remain a challenge in the field of neurosurgery.Since the first report of application of the extreme lateral supracerebellar infratentorial（ELSI）approach in resecting the PMJ lesions in 2000,a few articles concerning the ELSI approach have been published.The present review mainly provided an intimate introduction of the ELSI approach,and evalu- ated it in facets of patient position,skin incision,craniectomy,draining veins,retraction against the cerebellum,exposure limits,patient healing,as well as advantages and limitations compared with other approaches.The ELSI approach is proposed to be a very young and promising approach to access the lesions of posterolateral PMJ region and the posterolateral tentorial gap.Besides,it has several advantages such as having a shorter surgical pathway,causing less surgical complications,laborsaving,etc.Still,more studies are needed to improve this approach.
