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细脚拟青霉培养性状和药理作用的初步研究 被引量:25
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作者 陈祝安 徐珊 《真菌学报》 CSCD 北大核心 1989年第3期214-220,242,共7页
细脚拟青霉被认为是蛾蛹草(Cordyceps polyarthra Moller)的不完全阶段。在自然基物上生长最佳,容易培养。该菌生长最适温度为24—26℃,40℃分生孢子失活。无性世代周期观察表明,从孢子萌发到次代分生孢子产生仅24小时。药理试验结果,... 细脚拟青霉被认为是蛾蛹草(Cordyceps polyarthra Moller)的不完全阶段。在自然基物上生长最佳,容易培养。该菌生长最适温度为24—26℃,40℃分生孢子失活。无性世代周期观察表明,从孢子萌发到次代分生孢子产生仅24小时。药理试验结果,该菌对小鼠毒性口服剂量LD_(50)>70g/kg;腹腔注射 LD_(50)为17.6±1.78g/kg。并对小鼠常压耐缺氧能力、镇静、镇痛等作用进行了实验,和对照相比均有非常显著的差异。且毒性较低,可能成为一种有药用价值的资源菌。 展开更多
关键词 细脚拟青霉 蛾蛹草 培养性状 止痛
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Cecropin B抗菌肽基因的定向诱变与表达 被引量:21
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作者 胡云龙 胡泰山 +5 位作者 林世康 苏剑 施国民 李伟 赵学忠 屈贤铭 《药物生物技术》 CAS CSCD 1999年第4期193-197,共5页
采用寡核苷酸介导的定向点突变法,对天蚕抗菌肽Cecropin B基因13 位甲硫氨酸( Met) 诱变为亮氨酸(Leu) 或缬氨酸(Val) ,保留1 位上的Met,诱变后的Cecropin BDNA序列分析证明产生了预期的... 采用寡核苷酸介导的定向点突变法,对天蚕抗菌肽Cecropin B基因13 位甲硫氨酸( Met) 诱变为亮氨酸(Leu) 或缬氨酸(Val) ,保留1 位上的Met,诱变后的Cecropin BDNA序列分析证明产生了预期的点突变。将Cecropin B 突变体基因与pGEX4T2 融合表达载体中的谷胱甘肽转移酶(GST) 基因融合,在E.coli 中表达,当IPTG 诱导30min 后,工程菌的数量开始减少,然后逐渐恢复正常。说明Cecropin B 突变体与GST 基因融合表达后仍然具有很强杀伤原核细胞的作用。 展开更多
关键词 抗菌肽 定向诱变 基因表达 CECROPIN B
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花椒挥发油对两种人体蠕形螨的体外抑杀作用 被引量:14
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作者 蒋丽艳 刘继鑫 +4 位作者 张浩 孙艳宏 宋波 周芳烨 杜凤霞 《医学研究杂志》 2009年第2期78-79,共2页
目的观察花椒挥发油体外杀螨效果。方法采用透明胶带粘贴法获取蠕形螨,镜下观察花椒精油及不同浓度的花椒挥发油对两种人体蠕形螨的抑杀作用。结果花椒精油对两种蠕形螨均有明显的抑杀作用,且对皮质蠕形螨的抑杀效果优于毛囊蠕形螨,但... 目的观察花椒挥发油体外杀螨效果。方法采用透明胶带粘贴法获取蠕形螨,镜下观察花椒精油及不同浓度的花椒挥发油对两种人体蠕形螨的抑杀作用。结果花椒精油对两种蠕形螨均有明显的抑杀作用,且对皮质蠕形螨的抑杀效果优于毛囊蠕形螨,但随稀释倍数的增加抑杀效果明显降低。结论花椒精油具有良好的体外抑杀蠕形螨的作用。 展开更多
关键词 花椒挥发油 人体蠕形螨 抑杀
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Aircraft vulnerability modeling and computation methods based on product structure and CATIA 被引量:13
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作者 Li Jun Yang Wei +3 位作者 Zhang Yugang Pei Yang Ren Yunsong Wang Wei 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2013年第2期334-342,共9页
Survivability strengthening/vulnerability reduction designs have become one of the most important design disciplines of military aircraft now. Due to progressiveness and complexity of modern combat aircraft, the exist... Survivability strengthening/vulnerability reduction designs have become one of the most important design disciplines of military aircraft now. Due to progressiveness and complexity of modern combat aircraft, the existing vulnerability modeling and computation methods cannot meet the current engineering application requirements. Therefore, a vulnerability modeling and computation method based on product structure and CATIA is proposed in sufficient consideration of the design characteristics of modern combat aircraft. This method directly constructs the aircraft vulnerability model by CATIA or the digital model database, and manages all the product components of the vulnerability model via aircraft product structure. Using CAA second development, the detailed operations and computation methods of vulnerability analysis are integrated into CATIA software environment. Comprehensive assessment data and visual kill probability Iso-contours can also be presented, which meet the vulnerability analysis requirements of modern combat aircraft effectively. The intact vulnerability model of one hypothetical aircraft is constructed, and the effects of redundant technology to the aircraft vulnerability are assessed, which validate the engineering practicality of the method. 展开更多
关键词 Aircraft assembly Combat effectiveness CATIA kill probability Product structure VULNERABILITY REDUNDANCY
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谈羽毛球女子双打项目的扣杀与封网 被引量:10
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作者 潘莉 《南京体育学院学报(自然科学版)》 2004年第1期54-55,共2页
根据女运动员在羽毛球实战中扣杀和封网的不同效果,指出“快速、全面、攻守兼备”的打法已成为当前世界羽毛球女子双打项目技战术发展的方向。
关键词 羽毛球 女子双打 扣杀 封网 全面技术
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A Generic Calculation Model for Aircraft Single-hit Vulnerability Assessment Based on Equivalent Target 被引量:11
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作者 裴扬 宋笔锋 韩庆 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2006年第3期183-189,共7页
Aircraft single-hit vulnerability/survivability is usually expressed as the probability of kill or the vulnerable area in case of being given a random threat (e.g. a fragment) hit on the aircraft. In this paper, int... Aircraft single-hit vulnerability/survivability is usually expressed as the probability of kill or the vulnerable area in case of being given a random threat (e.g. a fragment) hit on the aircraft. In this paper, introducing the "equivalent target method" in lethality field into the aircraft vulnerability assessment, a generic vulnerability calculation model is proposed. In order for a good representation of reality, the model considers the threat change of state-of-motion during the threat penetrating into the components successively. Application shows that the proposed generic model has solved the Pk/h (probability of kill given by a hit on the component) calculation problem in aircraft vulnerability assessment, and is easier to be computerized than other commonly used models. 展开更多
关键词 VULNERABILITY equivalent target survivability probability of kill vulnerable area
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拓扑异构酶Ⅰ抑制剂对K562细胞的杀伤与诱导凋亡作用 被引量:13
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作者 陈协群 万幼峰 +1 位作者 白庆咸 曹云新 《癌症》 SCIE CAS CSCD 北大核心 2002年第4期356-359,共4页
背景与目的:近年发现,拓扑异构酶Ⅰ抑制剂对加速期或急变期的慢性髓细胞白血病有较好疗效。为了深入理解拓扑异构酶Ⅰ抑制剂这一新的药理作用,本研究采用具有慢性髓细胞白血病特征性异常染色体犤t(9;22)犦的K562细胞株为实验对象,进一... 背景与目的:近年发现,拓扑异构酶Ⅰ抑制剂对加速期或急变期的慢性髓细胞白血病有较好疗效。为了深入理解拓扑异构酶Ⅰ抑制剂这一新的药理作用,本研究采用具有慢性髓细胞白血病特征性异常染色体犤t(9;22)犦的K562细胞株为实验对象,进一步探讨拓扑异构酶Ⅰ抑制剂拓扑替康(topotecan)对靶细胞的杀伤与诱导凋亡活性。方法:采用MTT法测定拓扑替康对K562细胞的杀伤作用;通过形态学与AnnexinVFITC染色,研究拓扑替康对靶细胞的促凋亡活性;采用caspase-8特异性抑制剂IETD-fmk,分析拓扑替康介导的细胞杀伤或凋亡和caspase活化的关系。结果:经0.15μmol/L拓扑替康处理至12、24、48及72h时,K562细胞的存活率与对照相比,逐渐降至(92±36)%犤P>0.05vs(94±27)%犦、(68±21)%犤P<0.05vs(119±13)%犦、(54±15)%犤P<0.05vs(132±31)%犦及(21±10)%犤P<0.01vs(114±19)%犦;同时,靶细胞出现磷脂酰丝氨酸外翻、细胞固缩、染色质边集、核碎裂,最终解离为大量凋亡小体;经caspase-8抑制剂与拓扑替康联合处理至24、48h时,K562细胞的存活率依然维持在(95±29)%与(87±11)%,后者显著高于单用拓扑替康者犤P<0.05vs(54±15)%犦,且无明确的凋亡小体形成。 展开更多
关键词 拓扑替康 K562细胞 凋亡 杀伤 CASPASE-8 白血病 拓扑异构酶I抑制剂
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Physical Hydrography and Algal Bloom Transport in Hong Kong Waters 被引量:6
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作者 匡翠萍 李行伟 《China Ocean Engineering》 SCIE EI 2005年第4期539-556,共18页
In sub-tropical coastal waters around Hong Kong, algal blooms and red tides are usually first sighted in the Mirs Bay, in the eastern waters of Hong Kong. A calibrated three-dimensional hydrodynamic model for the Pear... In sub-tropical coastal waters around Hong Kong, algal blooms and red tides are usually first sighted in the Mirs Bay, in the eastern waters of Hong Kong. A calibrated three-dimensional hydrodynamic model for the Pearl River Estuary (Delft3D) has been applied to the study of the physical hydrography of Hong Kong waters and its relationship with algal bloom transport patterns in the dry and wet seasons. The general 3D hydrodynamic circulation and salinity structure in the partially-mixed estuary are presented. Extensive numerical surface drogue tracking experiments are performed for algal blooms that are initiated in the Mirs Bay under different seasonal, wind and tidal conditions. The probability of bloom impact on the Victoria Harbour and nearby urban coastal waters is estimated. The computations show that: i) In the wet season (May - August), algal blooms initiated in the Mirs Bay will move in a clockwise direction out of the bay, and be transported away from Hong Kong due to SW monsoon winds which drive the SW to NE coastal current; ii) In the dry season (November- April), algal blooms initiated in the northeast Mirs Bay will move in an anti-clockwise direction and be carried away into southern waters due to the NE to SW coastal current driven by the NE monsoon winds; the bloom typically flows past the east edge of the Victoria Harbeur and nearby waters. Finally, the role of hydrodynamic transport in an important episodic event -- the spring 1998 massive red tide -- is quantitatively examined. It is shown that the strong NE to E wind during late March to early April, coupled with the diurnal tide at the beginning of April, significantly increased the probability of bloom transport into the Port Shelter and East Lamma Channel, resulting in the massive fish kill. The results provide a basis for risk assessment of harmful algal bloom (HAB) impact on urban coastal waters around the Victoria Habour. 展开更多
关键词 red tide harmful algal blooms MARICULTURE fish kill drogue tracking EUTROPHICATION hydrodynamic modelling water quality environmental hydraulics wind-induced transport
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不同杀青方法对黄花菜外观品质及干制率的影响研究 被引量:9
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作者 李登绚 李东波 +3 位作者 胥国斌 曹刘英 李彩霞 周强 《陇东学院学报》 2012年第5期32-34,共3页
采用太阳能、蒸汽、微波、药物等四种不同杀青方法处理黄花菜,按照国家标准,对比研究分析其外观品质:色泽、气味、形状、肉质级别及出干率,其结果为:太阳能杀青干制的黄花菜,条色均匀,金黄,有光泽,肉质肥厚,无霉味或其它异味,级别特级,... 采用太阳能、蒸汽、微波、药物等四种不同杀青方法处理黄花菜,按照国家标准,对比研究分析其外观品质:色泽、气味、形状、肉质级别及出干率,其结果为:太阳能杀青干制的黄花菜,条色均匀,金黄,有光泽,肉质肥厚,无霉味或其它异味,级别特级,含水量10.77%.干制率13.84%最高.由此可见,太阳能杀青干制黄花菜外观品质最优,优选的最佳干制工艺为:太阳能杀青:温度60-75℃,光照强度为2800-3000lx,时间3-4h. 展开更多
关键词 杀青 黄花菜 外观品质 干制率 比较
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夏秋绿茶的杀青工艺及加工设备研究
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作者 杨莹 黄泽界 +2 位作者 何端 冯文颖 龙开进 《现代食品》 2024年第12期110-112,共3页
本项目基于对贵州省夏秋茶的前期研究基础,通过结合先进的加工工艺和高效智能的加工设备可以生产出数量可观的优质绿茶。这种绿茶具有丰富的内含物,耐泡、鲜爽醇厚、香气高雅、汤色亮绿且安全可靠。通过这一项目,可以解决贵州省茶产业... 本项目基于对贵州省夏秋茶的前期研究基础,通过结合先进的加工工艺和高效智能的加工设备可以生产出数量可观的优质绿茶。这种绿茶具有丰富的内含物,耐泡、鲜爽醇厚、香气高雅、汤色亮绿且安全可靠。通过这一项目,可以解决贵州省茶产业能效不足、茶农收入不高等问题。 展开更多
关键词 夏秋绿茶 杀青 新型隧道式高效杀青机
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金黄色葡萄球菌生物被膜的控制方法研究进展 被引量:8
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作者 孙纪录 陈小雪 韩北忠 《中国酿造》 CAS 北大核心 2011年第6期1-3,共3页
金黄色葡萄球菌是一种重要的食源性病原菌。金黄色葡萄球菌能够形成生物被膜,使其更加难于根除。文中对金黄色葡萄球菌生物被膜的控制方法进行了全面综述,包括去除已形成的生物被膜、杀灭生物被膜中的金黄色葡萄球菌和抑制生物被膜的形... 金黄色葡萄球菌是一种重要的食源性病原菌。金黄色葡萄球菌能够形成生物被膜,使其更加难于根除。文中对金黄色葡萄球菌生物被膜的控制方法进行了全面综述,包括去除已形成的生物被膜、杀灭生物被膜中的金黄色葡萄球菌和抑制生物被膜的形成3个主要方面,旨在为食品工业领域生物被膜的控制提供借鉴。 展开更多
关键词 金黄色葡萄球菌 生物被膜 抑制 去除 杀灭
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不同杀青方法对黄花菜营养成分的影响 被引量:8
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作者 李登绚 李东波 +3 位作者 胥国斌 曹刘英 李彩霞 周强 《中国蔬菜》 北大核心 2011年第14期77-79,共3页
采用太阳能、蒸汽、微波、药物4种方法处理黄花菜,研究不同杀青方法对黄花菜营养成分的影响。结果表明:蒸汽杀青处理的黄花菜总糖和蛋白质含量高于国家标准,分别为55.66%、11.54%;太阳能杀青处理的黄花菜蛋白质含量(11.35%)高于国家标准... 采用太阳能、蒸汽、微波、药物4种方法处理黄花菜,研究不同杀青方法对黄花菜营养成分的影响。结果表明:蒸汽杀青处理的黄花菜总糖和蛋白质含量高于国家标准,分别为55.66%、11.54%;太阳能杀青处理的黄花菜蛋白质含量(11.35%)高于国家标准,VC(489.0mg.kg-1)和β-胡萝卜素含量(36.5mg.kg-1)最高,而总酸度(2.90%)低于国家标准,干制黄花菜品质最优,营养成分流失最少,加工简便,且节能环保。 展开更多
关键词 杀青 黄花菜 营养成分
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Beyond Defense: Proactive Approaches to Disaster Recovery and Threat Intelligence in Modern Enterprises
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作者 Meysam Tahmasebi 《Journal of Information Security》 2024年第2期106-133,共28页
As cyber threats keep changing and business environments adapt, a comprehensive approach to disaster recovery involves more than just defensive measures. This research delves deep into the strategies required to respo... As cyber threats keep changing and business environments adapt, a comprehensive approach to disaster recovery involves more than just defensive measures. This research delves deep into the strategies required to respond to threats and anticipate and mitigate them proactively. Beginning with understanding the critical need for a layered defense and the intricacies of the attacker’s journey, the research offers insights into specialized defense techniques, emphasizing the importance of timely and strategic responses during incidents. Risk management is brought to the forefront, underscoring businesses’ need to adopt mature risk assessment practices and understand the potential risk impact areas. Additionally, the value of threat intelligence is explored, shedding light on the importance of active engagement within sharing communities and the vigilant observation of adversary motivations. “Beyond Defense: Proactive Approaches to Disaster Recovery and Threat Intelligence in Modern Enterprises” is a comprehensive guide for organizations aiming to fortify their cybersecurity posture, marrying best practices in proactive and reactive measures in the ever-challenging digital realm. 展开更多
关键词 Advanced Persistent Threats (APT) Attack Phases Attack Surface DEFENSE-IN-DEPTH Disaster Recovery (DR) Incident Response Plan (IRP) Intrusion Detection Systems (IDS) Intrusion Prevention System (IPS) Key Risk Indicator (KRI) Layered Defense Lockheed Martin kill Chain Proactive Defense Redundancy Risk Management Threat Intelligence
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唐律中的“杀”与“死” 被引量:7
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作者 刘晓林 《政法论坛》 CSSCI 北大核心 2020年第3期93-102,共10页
《唐律疏议》中"杀"与"死"皆为广泛出现的高频词汇,两者在篇目与律条中的分布呈现重合。结合其作为立法语言的专门含义与用法,我们清晰地看到,法典中分布重合的主要表现是:作为犯罪行为的"杀"与作为行为... 《唐律疏议》中"杀"与"死"皆为广泛出现的高频词汇,两者在篇目与律条中的分布呈现重合。结合其作为立法语言的专门含义与用法,我们清晰地看到,法典中分布重合的主要表现是:作为犯罪行为的"杀"与作为行为结果的"死"在定罪量刑过程中的密切关系。唐律中"杀就是死,死并非杀",即行为对象死亡是作为犯罪行为的"杀"表意本身所包含的当然含义,立法不会单独描述作为杀人犯罪结果的死亡;若立法列举了致人"死亡"的行为,则此种行为并非"杀人"。此种关系渊源于秦汉律中的"杀就是死,死就是杀"。致人"死亡"的行为逐渐从"杀人"中分化出来,在刑事法律规范体系中具有了独立的表达方式与相应的定罪量刑地位,背后蕴含的是行为人主观心态对于定罪量刑的影响逐渐增强。 展开更多
关键词 唐律疏议 罪刑关系
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Prescribed performance neural control to guarantee tracking quality for near space kinetic kill vehicle 被引量:5
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作者 ZHANG Tao LI Jiong +2 位作者 LI Weimin WANG Huaji LEI Humin 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2019年第3期573-586,共14页
A prescribed performance neural controller to guarantee tracking quality is addressed for the near space kinetic kill vehicle (NSKKV) to meet the state constraints caused by side window detection. Different from the t... A prescribed performance neural controller to guarantee tracking quality is addressed for the near space kinetic kill vehicle (NSKKV) to meet the state constraints caused by side window detection. Different from the traditional prescribed performance control in which the shape of the performance function is constant, this paper exploits new performance functions which can change the shape of their function according to different symbols of initial errors and can ensure the error convergence with a small overshoot. The neural backstepping control and the minimal learning parameters (MLP) technology are employed for exploring a prescribed performance controller (PPC) that provides robust tracking attitude reference trajectories. The highlight is that the transient performance of tracking errors is satisfactory and the computational load of neural approximation is low. The pseudo rate (PSR) modulator is used to shape the continuous control command to pulse or on-off signals to meet the requirements of the thruster. Numerical simulations show that the proposed method can achieve state constraints, pseudo-linear operation and high accuracy. 展开更多
关键词 PRESCRIBED PERFORMANCE control near space kinetic kill vehicle (NSKKV) neural approximation minimal learning parameter (MLP) pseudo rate (PSR) MODULATOR
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基于Cyber Kill Chain的铁路信息网络安全防御研究 被引量:5
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作者 杨钰杰 霍云龙 《铁路计算机应用》 2021年第11期64-67,共4页
为有效应对铁路企业面临的日益严峻的信息网络安全威胁,引入Cyber Kill Chain模型来识别和预防网络入侵活动,分析了该模型7个阶段相应的攻击行为特点,针对各阶段的预期目的,提出符合铁路行业特点的信息网络安全防御措施,以求在网络攻击... 为有效应对铁路企业面临的日益严峻的信息网络安全威胁,引入Cyber Kill Chain模型来识别和预防网络入侵活动,分析了该模型7个阶段相应的攻击行为特点,针对各阶段的预期目的,提出符合铁路行业特点的信息网络安全防御措施,以求在网络攻击较早阶段瓦解网络威胁,构建全方位、深层次的网络安全防御体系,保护铁路基础设施、应用系统、数据资源等免遭网络攻击破坏。 展开更多
关键词 网络安全 Cyber kill Chain 安全防御 威胁分析 安全检测
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A framework of force of information influence and application for C4KISR system
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作者 MAO Shaojie DIAO Lianwang +6 位作者 SUN Yu WANG Heng YI Kan XU Xin MAO Xiaobin ZHANG Kecheng SHENG Long 《Journal of Systems Engineering and Electronics》 SCIE CSCD 2024年第2期430-443,共14页
The subversive nature of information war lies not only in the information itself, but also in the circulation and application of information. It has always been a challenge to quantitatively analyze the function and e... The subversive nature of information war lies not only in the information itself, but also in the circulation and application of information. It has always been a challenge to quantitatively analyze the function and effect of information flow through command, control, communications, computer, kill, intelligence,surveillance, reconnaissance (C4KISR) system. In this work, we propose a framework of force of information influence and the methods for calculating the force of information influence between C4KISR nodes of sensing, intelligence processing,decision making and fire attack. Specifically, the basic concept of force of information influence between nodes in C4KISR system is formally proposed and its mathematical definition is provided. Then, based on the information entropy theory, the model of force of information influence between C4KISR system nodes is constructed. Finally, the simulation experiments have been performed under an air defense and attack scenario. The experimental results show that, with the proposed force of information influence framework, we can effectively evaluate the contribution of information circulation through different C4KISR system nodes to the corresponding tasks. Our framework of force of information influence can also serve as an effective tool for the design and dynamic reconfiguration of C4KISR system architecture. 展开更多
关键词 information warfare command control communications computer kill intelligence surveillance reconnaissance(C4KISR)system information circulation force of information influence information entropy
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Autonomy Evaluation of Unmanned Systems Based on Task Models
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作者 Yi Zou Zehao Ni +1 位作者 Xun Lei Chi Zhang 《Machine Intelligence Research》 EI CSCD 2024年第5期815-830,共16页
In this study,relevant work on autonomy evaluation(AE)in recent years was comprehensively reviewed and classified from the perspective of task models,and a closed-loop task models based theoretical framework for AE wa... In this study,relevant work on autonomy evaluation(AE)in recent years was comprehensively reviewed and classified from the perspective of task models,and a closed-loop task models based theoretical framework for AE was developed.The main contributions of this study are as follows.1)A taxonomy for AE based on task models was introduced to classify current theories,methods and standards.2)The limitations of the current autonomous evaluation methods were addressed to provide a theoretical framework for quantitative evaluation based on task models,and evaluation metrics for each stage were proposed based on the AE theoretical framework.3)Qualitative analyses of the superiority of the proposed AE framework based on the closed-loop task models were conducted.This study attempts to provide a reference for researchers and engineers in the autonomous unmanned systems field and inspire future development of AE. 展开更多
关键词 Unmanned system(UMS) autonomy evaluation task models closed-loop task models kill chain
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杀灭与中和:中西医治疗癌症的差异 被引量:5
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作者 李德顺 程井军 《医学争鸣》 北大核心 2017年第3期24-27,32,共5页
从哲学文化源头入手,比较中西医治疗癌症模式的差异,对两种不同的思维进行反思,以期加深对其内涵的认识,为肿瘤治疗的临床决策提供参考。研究认为:两种治疗癌症模式的差异可以概括为"杀灭"与"中和"。前者植根于海... 从哲学文化源头入手,比较中西医治疗癌症模式的差异,对两种不同的思维进行反思,以期加深对其内涵的认识,为肿瘤治疗的临床决策提供参考。研究认为:两种治疗癌症模式的差异可以概括为"杀灭"与"中和"。前者植根于海洋文化,以征服自然为哲学基础,以完全杀灭癌细胞为目标;后者植根于农耕文化,以天人合一为哲学基础,以调和脏腑、气血、经络为目标。笔者反思认为:西医的"杀灭"思维在临床中具有很大的局限性,是不可能实现的,中医的"中和"思维应该成为未来的主导模式。 展开更多
关键词 杀灭 中和 中西医 生存质量
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医用臭氧对肝癌细胞HepG2生物学行为的实验研究 被引量:5
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作者 周玉川 刘康 +4 位作者 钟立明 杨巧丽 游箭 魏欣 邓川 《西部医学》 2016年第4期465-468,共4页
目的探讨使用医用臭氧(O3)在体外对肝癌HepG2细胞的作用。方法获取人肝癌HepG2细胞在体外增殖、传代、对数生长期的细胞,将细胞分为对照组和实验组,实验组采用含浓度为0.5μg/ml的医用臭氧水的培养液处理细胞,对比两组试验的细胞形态学... 目的探讨使用医用臭氧(O3)在体外对肝癌HepG2细胞的作用。方法获取人肝癌HepG2细胞在体外增殖、传代、对数生长期的细胞,将细胞分为对照组和实验组,实验组采用含浓度为0.5μg/ml的医用臭氧水的培养液处理细胞,对比两组试验的细胞形态学变化,细胞生长曲线、细胞凋亡情况、细胞迁移能力变化。结果体外实验结果显示,含臭氧的培养基中细胞数量明显减少,肿瘤细胞膜破裂,核固缩甚至裂解,臭氧对细胞的生长有抑制作用,同时CCK-8的检测结果显示O3能抑制肝癌细胞的增殖(P<0.01),Transwell迁移实验显示O3能抑制肿瘤细胞的迁移(P<0.01)。结论臭氧能直接杀死肝癌HepG2细胞,能在体外抑制肝癌HepG2细胞的增殖和迁移。 展开更多
关键词 臭氧 肝癌HEPG2细胞 抑制作用 杀伤
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