目的:观察海风藤酮对急性胰腺炎(AP)大鼠自由基水平的影响．方法:♂SD大鼠120只随机分为对照组(n= 40),AP组(n=40)和海风藤酮(SM)治疗组(n=40)．观察3,6,12和24 h大鼠血浆丙二醛(MDA)、超氧化物歧化酶(SOD)、淀粉酶(AMY)、腹水量、胰腺...目的:观察海风藤酮对急性胰腺炎(AP)大鼠自由基水平的影响．方法:♂SD大鼠120只随机分为对照组(n= 40),AP组(n=40)和海风藤酮(SM)治疗组(n=40)．观察3,6,12和24 h大鼠血浆丙二醛(MDA)、超氧化物歧化酶(SOD)、淀粉酶(AMY)、腹水量、胰腺病理等指标的变化．结果:与对照组比较,AP组腹水、血清AMY和MDA升高,SOD水平降低,且有差异统计学意义,经海风藤酮治疗后,治疗组腹水(24 h: 4．03±1．36 mL vs 5．15±1．69 mL,P＜0．05),血清AMY(24 h:69．6±6．7μkat/L vs 136．6±13．4 ukat/L,P＜0．01)和MDA(24 h:1．45±0．19 nmol/ L vs 1．98±0．27 nmol/L,P＜0．05)水平显著降低, SOD水平显著升高(24 h:35．27±8．34 nmol/L vs 19．03±8．74 nmol/L,P＜0．05)．AP组大鼠胰腺病理变化均显著性升高,而治疗组与AP组相比在早期(3,6 h)胰腺病理变化明显减轻．结论:海风藤酮通过降低损害因子MDA,升高保护因子SOD对AP有治疗作用．展开更多
Objective： To study the effects of kadsurenone on inflammatory mediators Platelet-activating factor （PAF）, tumor necrosis factor-α（TNF-α） and interleukin-6（IL-6） in rat model of acute pancreatitis（AP）. Meth...Objective： To study the effects of kadsurenone on inflammatory mediators Platelet-activating factor （PAF）, tumor necrosis factor-α（TNF-α） and interleukin-6（IL-6） in rat model of acute pancreatitis（AP）. Methods： SD male rats （104） were randomly divided into sham group （n = 24）, AP group （n = 40） and kadsurenone group （n = 40）. The rats were killed 3, 6, 12 and 24 hours after operation. The serum level of PAF, TNF-α and IL-6 was measured. Results： The serum level of PAF, TNF-α and IL-6 of AP group was significantly increased（P 〈 0.01 ）compared with control group. The serum level of TNF-α got to a peak 6 hours after operation, and the serum IL-6 getting to a peak 12 hours after operation in AP group. After kadsurenone was administered to AP rats, pancreas and lung myeloperoxidase （MPO）, serum amylase activity was reduced. Histology showed a trend toward improvement. The serum level of PAF, TNF-α and IL-6 was significantly decreased （P 〈 0.05）. Conclusion. Kadsurenone can reduce the severity of systemic inflammation in rats with AP and relieve the damage of the pancreas and lung in AP rats. These results suggested that kadsurenone may be useful in the treatment of acute pancreatitis.展开更多
文摘目的:观察海风藤酮对急性胰腺炎(AP)大鼠自由基水平的影响．方法:♂SD大鼠120只随机分为对照组(n= 40),AP组(n=40)和海风藤酮(SM)治疗组(n=40)．观察3,6,12和24 h大鼠血浆丙二醛(MDA)、超氧化物歧化酶(SOD)、淀粉酶(AMY)、腹水量、胰腺病理等指标的变化．结果:与对照组比较,AP组腹水、血清AMY和MDA升高,SOD水平降低,且有差异统计学意义,经海风藤酮治疗后,治疗组腹水(24 h: 4．03±1．36 mL vs 5．15±1．69 mL,P＜0．05),血清AMY(24 h:69．6±6．7μkat/L vs 136．6±13．4 ukat/L,P＜0．01)和MDA(24 h:1．45±0．19 nmol/ L vs 1．98±0．27 nmol/L,P＜0．05)水平显著降低, SOD水平显著升高(24 h:35．27±8．34 nmol/L vs 19．03±8．74 nmol/L,P＜0．05)．AP组大鼠胰腺病理变化均显著性升高,而治疗组与AP组相比在早期(3,6 h)胰腺病理变化明显减轻．结论:海风藤酮通过降低损害因子MDA,升高保护因子SOD对AP有治疗作用．
基金By the Science Fund of health department of Shan xi Province (NO.2004D41)
文摘Objective： To study the effects of kadsurenone on inflammatory mediators Platelet-activating factor （PAF）, tumor necrosis factor-α（TNF-α） and interleukin-6（IL-6） in rat model of acute pancreatitis（AP）. Methods： SD male rats （104） were randomly divided into sham group （n = 24）, AP group （n = 40） and kadsurenone group （n = 40）. The rats were killed 3, 6, 12 and 24 hours after operation. The serum level of PAF, TNF-α and IL-6 was measured. Results： The serum level of PAF, TNF-α and IL-6 of AP group was significantly increased（P 〈 0.01 ）compared with control group. The serum level of TNF-α got to a peak 6 hours after operation, and the serum IL-6 getting to a peak 12 hours after operation in AP group. After kadsurenone was administered to AP rats, pancreas and lung myeloperoxidase （MPO）, serum amylase activity was reduced. Histology showed a trend toward improvement. The serum level of PAF, TNF-α and IL-6 was significantly decreased （P 〈 0.05）. Conclusion. Kadsurenone can reduce the severity of systemic inflammation in rats with AP and relieve the damage of the pancreas and lung in AP rats. These results suggested that kadsurenone may be useful in the treatment of acute pancreatitis.
