Fluorescence lifetime imaging can reveal the high-resolution structure of various biophysical and chemical parameters in a microenvironment quantitatively.However,the depth of imaging is generally limited to hundreds ...Fluorescence lifetime imaging can reveal the high-resolution structure of various biophysical and chemical parameters in a microenvironment quantitatively.However,the depth of imaging is generally limited to hundreds of micrometers due to aberration and light scattering in biological tissues.This paper introduces an iterative multi-photon adaptive compensation technique(IMPACT)into a two-photon fluorescence lifetime microscopy system to successfully overcome aberrations and multiple scattering problems in deep tissues.It shows that 400 correction modes can be achieved within 5 min,which was mainly limited by the frame rate of a spatial light modulator.This system was used for high-resolution imaging of mice brain tissue and live zebrafish,further verifying its superior performance in imaging quality and photon accumulation speed.展开更多
For measurement of component content in the extraction and separation process of praseodymium/neodymium(Pr/Nd), a soft measurement method was proposed based on modeling of ion color features, which is suitable for fas...For measurement of component content in the extraction and separation process of praseodymium/neodymium(Pr/Nd), a soft measurement method was proposed based on modeling of ion color features, which is suitable for fast estimation of component content in production field. Feature analysis on images of the solution is conducted,which are captured from Pr/Nd extraction/separation field. H/S components in the HSI color space are selected as model inputs, so as to establish the least squares support vector machine(LSSVM) model for Nd(Pr) content,while the model parameters are determined with the GA algorithm. To improve the adaptability of the model,the adaptive iteration algorithm is used to correct parameters of the LSSVM model, on the basis of model correction strategy and new sample data. Using the field data collected from rare earth extraction production, predictive methods for component content and comparisons are given. The results indicate that the proposed method presents good adaptability and high prediction precision, so it is applicable to the fast detection of element content in the rare earth extraction.展开更多
The chemical composition of alloys directly determines their mechanical behaviors and application fields.Accurate and rapid analysis of both major and minor elements in alloys plays a key role in metallurgy quality co...The chemical composition of alloys directly determines their mechanical behaviors and application fields.Accurate and rapid analysis of both major and minor elements in alloys plays a key role in metallurgy quality control and material classification processes.A quantitative calibration-free laser-induced breakdown spectroscopy(CF-LIBS)analysis method,which carries out combined correction of plasma temperature and spectral intensity by using a secondorder iterative algorithm and two boundary standard samples,is proposed to realize accurate composition measurements.Experimental results show that,compared to conventional CF-LIBS analysis,the relative errors for major elements Cu and Zn and minor element Pb in the copperlead alloys has been reduced from 12%,26%and 32%to 1.8%,2.7%and 13.4%,respectively.The measurement accuracy for all elements has been improved substantially.展开更多
基金supported by the National Key Research and Development Program of China(No.2021YFF0502900)the National Natural Science Foundation of China(Nos.62175163,62225505,61935012,61835009,62127819,and 62205220)+2 种基金the Shenzhen Key Projects(No.JCYJ20200109105404067)the Shenzhen Talent Innovation Project(No.RCJC20210706091949022)the Shenzhen Science and Technology Planning Project(No.ZDSYS20210623092006020)。
文摘Fluorescence lifetime imaging can reveal the high-resolution structure of various biophysical and chemical parameters in a microenvironment quantitatively.However,the depth of imaging is generally limited to hundreds of micrometers due to aberration and light scattering in biological tissues.This paper introduces an iterative multi-photon adaptive compensation technique(IMPACT)into a two-photon fluorescence lifetime microscopy system to successfully overcome aberrations and multiple scattering problems in deep tissues.It shows that 400 correction modes can be achieved within 5 min,which was mainly limited by the frame rate of a spatial light modulator.This system was used for high-resolution imaging of mice brain tissue and live zebrafish,further verifying its superior performance in imaging quality and photon accumulation speed.
基金Supported by the National Natural Science Foundation of China(51174091,61364013,61164013)Earlier Research Project of the State Key Development Program for Basic Research of China(2014CB360502)
文摘For measurement of component content in the extraction and separation process of praseodymium/neodymium(Pr/Nd), a soft measurement method was proposed based on modeling of ion color features, which is suitable for fast estimation of component content in production field. Feature analysis on images of the solution is conducted,which are captured from Pr/Nd extraction/separation field. H/S components in the HSI color space are selected as model inputs, so as to establish the least squares support vector machine(LSSVM) model for Nd(Pr) content,while the model parameters are determined with the GA algorithm. To improve the adaptability of the model,the adaptive iteration algorithm is used to correct parameters of the LSSVM model, on the basis of model correction strategy and new sample data. Using the field data collected from rare earth extraction production, predictive methods for component content and comparisons are given. The results indicate that the proposed method presents good adaptability and high prediction precision, so it is applicable to the fast detection of element content in the rare earth extraction.
基金financially supported by the National Key Research and Development Program of China(No.2017YFA0304203)the Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China(No.IRT13076)+3 种基金National Natural Science Foundation of China(Nos.61475093,61378047,61775125)the Shanxi‘1331 Project’Key Subjects Constructionthe Major Special Science and Technology Projects in Shanxi Province(No.MD2016-01)the State Key Lab of Power Systems for technical contribution and financial support
文摘The chemical composition of alloys directly determines their mechanical behaviors and application fields.Accurate and rapid analysis of both major and minor elements in alloys plays a key role in metallurgy quality control and material classification processes.A quantitative calibration-free laser-induced breakdown spectroscopy(CF-LIBS)analysis method,which carries out combined correction of plasma temperature and spectral intensity by using a secondorder iterative algorithm and two boundary standard samples,is proposed to realize accurate composition measurements.Experimental results show that,compared to conventional CF-LIBS analysis,the relative errors for major elements Cu and Zn and minor element Pb in the copperlead alloys has been reduced from 12%,26%and 32%to 1.8%,2.7%and 13.4%,respectively.The measurement accuracy for all elements has been improved substantially.
