邻二氮菲是一种常用的氧化还原指示剂,本文主要对邻二氮菲比色法测定铁含量的条件优化进行了研究,利用优化的实验条件测定了多维铁口服溶液中的铁含量。实验采用邻二氮菲显色、盐酸羟胺还原及标准曲线法测定铁浓度,得出了最优实验条件:...邻二氮菲是一种常用的氧化还原指示剂,本文主要对邻二氮菲比色法测定铁含量的条件优化进行了研究,利用优化的实验条件测定了多维铁口服溶液中的铁含量。实验采用邻二氮菲显色、盐酸羟胺还原及标准曲线法测定铁浓度,得出了最优实验条件:波长(510 nm)、显色剂用量(2 m L)、醋酸钠用量(3 m L)、显色时间(10 min)。同时,对不同试剂添加顺序进行比较研究,提高了分析检测的灵敏度。展开更多
Iron is an essential nutrient for plant metabolism such that Fe-limited plants display chlorosis and suffer from reduced photosynthetic efficiency. Differential display previously identified genes whose expression was...Iron is an essential nutrient for plant metabolism such that Fe-limited plants display chlorosis and suffer from reduced photosynthetic efficiency. Differential display previously identified genes whose expression was elevated in Fe-deficient maize roots. Here,we describe the functional characterization of one of the genes identified in the screen,ZmFDR3 (Zea maize Fe-deficiency-related). Heterologous functional complementation assays using a yeast iron uptake mutant showed that ZmFDR3 functions in iron transport. ZmFDR3 contains a domain found in FliN-proteins of the type III secretion system and is predicted to localize to the thylakoid of plastids. Fluorescence immunocytochemistry showed that ZmFDR3 is localized in the plastids of roots,stems and leaves,with high expression found in guard cell chloroplasts. Transgenic tobacco expressing a 35S-ZmFDR3 construct contains elevated iron content,displays well arranged thylakoid membranes and has photosynthetic indices that are higher than those of the wild type. Together,these results suggest that ZmFDR3 functions in chloroplast iron transport.展开更多
文摘邻二氮菲是一种常用的氧化还原指示剂,本文主要对邻二氮菲比色法测定铁含量的条件优化进行了研究,利用优化的实验条件测定了多维铁口服溶液中的铁含量。实验采用邻二氮菲显色、盐酸羟胺还原及标准曲线法测定铁浓度,得出了最优实验条件:波长(510 nm)、显色剂用量(2 m L)、醋酸钠用量(3 m L)、显色时间(10 min)。同时,对不同试剂添加顺序进行比较研究,提高了分析检测的灵敏度。
基金Supported by the National Natural Science Foundation (Grant No. 30770178)Beijing Municipal Natural Science Foundation Key Fund Projects (B) (Grant No. KZ200710028013)
文摘Iron is an essential nutrient for plant metabolism such that Fe-limited plants display chlorosis and suffer from reduced photosynthetic efficiency. Differential display previously identified genes whose expression was elevated in Fe-deficient maize roots. Here,we describe the functional characterization of one of the genes identified in the screen,ZmFDR3 (Zea maize Fe-deficiency-related). Heterologous functional complementation assays using a yeast iron uptake mutant showed that ZmFDR3 functions in iron transport. ZmFDR3 contains a domain found in FliN-proteins of the type III secretion system and is predicted to localize to the thylakoid of plastids. Fluorescence immunocytochemistry showed that ZmFDR3 is localized in the plastids of roots,stems and leaves,with high expression found in guard cell chloroplasts. Transgenic tobacco expressing a 35S-ZmFDR3 construct contains elevated iron content,displays well arranged thylakoid membranes and has photosynthetic indices that are higher than those of the wild type. Together,these results suggest that ZmFDR3 functions in chloroplast iron transport.
