利用生态调控、人工促熟和催产技术对菊黄东方鲀（♀）与红鳍东方鲀（♂）进行种间杂交，并对杂交子代胚胎及仔稚鱼发育进行了系统观察，详细描述了胚胎发育时序、形态特征以及仔稚幼鱼的发育特点。研究结果表明，菊黄东方鲀（♀）×...利用生态调控、人工促熟和催产技术对菊黄东方鲀（♀）与红鳍东方鲀（♂）进行种间杂交，并对杂交子代胚胎及仔稚鱼发育进行了系统观察，详细描述了胚胎发育时序、形态特征以及仔稚幼鱼的发育特点。研究结果表明，菊黄东方鲀（♀）×红鳍东方鲀（♂）杂交子代受精卵为沉性、具弱黏性，卵径（1．02±0．04） m m。在水温18～19℃、盐度30～32条件下，杂交子代受精卵经过150 h发育为初孵仔鱼，整个胚胎发育需有效积温2790℃· h ，其发育过程分7个阶段，24个发育分期。初孵仔鱼全长（2．52±0．15）mm，卵黄囊约占全长1／3，肌节和血管明显；孵化后4d，卵黄囊大部分被吸收，开始由内源性营养向外源性营养过渡，仔鱼具摄食能力。8 d后，卵黄囊完全被吸收。15 d后，仔鱼腹部前方出现大量“小皮刺”，开始出现鼓气现象。18 d后，齿形成，进入稚鱼期。25 d ，齿板形成，出现互残攻击现象，进入稚鱼死亡高峰期。40 d后，进入幼鱼期，其形态特征偏向于菊黄东方鲀。展开更多
The circadian rhythms of apical meristem mitosis cells of the two types of soybean were investigated： wild soybeans - Glycine max （L.） Merr. （G. hispida Max.）, G. soja Sieb., and Zucc （G, ussuriensis Reg. and Ma...The circadian rhythms of apical meristem mitosis cells of the two types of soybean were investigated： wild soybeans - Glycine max （L.） Merr. （G. hispida Max.）, G. soja Sieb., and Zucc （G, ussuriensis Reg. and Maack）; and the hybrids of their homozygous forms （F6）. Representatives of the initial kinds and interspecific hybrids for reproduction were grown up in comparable conditions. Seeds of a soybean were grown up at 24℃ in Petri cups in thermostat in darkness during winter. For research of mitotic meristem cages activity the tips of roots were fixed temporal by acetoalcohol （fractional fixing） from 1 p.m. till 12 a.m., further up to 12 p.m. each 60 minutes. Acetokarmin was used for preparing the coloring. For researching of mitotic indexes, there have been analyzed from 6 up to 10 thousand cells at each stage （during each moment of time： 01 hour, 02, 03 06 ... 12, 13 ... 18 hours and further）. Within a day （day ＋ night） in cells of root apical meristem, three mitosis ＂waves＂ in each of the investigated forms with an absolute starting point of rest at 12 p.m., with precisely expressed further periodicity were found out. Each of the investigated forms misses in character of wave processes, on a degree of increase and recession of mitosis waves, on the level of mitotic indexes. Maximal mitotic activity at all the investigated forms is noted at 6 and 12 a.m. and 6 p.m.展开更多
文摘利用生态调控、人工促熟和催产技术对菊黄东方鲀（♀）与红鳍东方鲀（♂）进行种间杂交，并对杂交子代胚胎及仔稚鱼发育进行了系统观察，详细描述了胚胎发育时序、形态特征以及仔稚幼鱼的发育特点。研究结果表明，菊黄东方鲀（♀）×红鳍东方鲀（♂）杂交子代受精卵为沉性、具弱黏性，卵径（1．02±0．04） m m。在水温18～19℃、盐度30～32条件下，杂交子代受精卵经过150 h发育为初孵仔鱼，整个胚胎发育需有效积温2790℃· h ，其发育过程分7个阶段，24个发育分期。初孵仔鱼全长（2．52±0．15）mm，卵黄囊约占全长1／3，肌节和血管明显；孵化后4d，卵黄囊大部分被吸收，开始由内源性营养向外源性营养过渡，仔鱼具摄食能力。8 d后，卵黄囊完全被吸收。15 d后，仔鱼腹部前方出现大量“小皮刺”，开始出现鼓气现象。18 d后，齿形成，进入稚鱼期。25 d ，齿板形成，出现互残攻击现象，进入稚鱼死亡高峰期。40 d后，进入幼鱼期，其形态特征偏向于菊黄东方鲀。
文摘The circadian rhythms of apical meristem mitosis cells of the two types of soybean were investigated： wild soybeans - Glycine max （L.） Merr. （G. hispida Max.）, G. soja Sieb., and Zucc （G, ussuriensis Reg. and Maack）; and the hybrids of their homozygous forms （F6）. Representatives of the initial kinds and interspecific hybrids for reproduction were grown up in comparable conditions. Seeds of a soybean were grown up at 24℃ in Petri cups in thermostat in darkness during winter. For research of mitotic meristem cages activity the tips of roots were fixed temporal by acetoalcohol （fractional fixing） from 1 p.m. till 12 a.m., further up to 12 p.m. each 60 minutes. Acetokarmin was used for preparing the coloring. For researching of mitotic indexes, there have been analyzed from 6 up to 10 thousand cells at each stage （during each moment of time： 01 hour, 02, 03 06 ... 12, 13 ... 18 hours and further）. Within a day （day ＋ night） in cells of root apical meristem, three mitosis ＂waves＂ in each of the investigated forms with an absolute starting point of rest at 12 p.m., with precisely expressed further periodicity were found out. Each of the investigated forms misses in character of wave processes, on a degree of increase and recession of mitosis waves, on the level of mitotic indexes. Maximal mitotic activity at all the investigated forms is noted at 6 and 12 a.m. and 6 p.m.
