目的观察透明质酸钠注射液联合塞来昔布胶囊对膝骨关节炎(knee osteoarthritis,KOA)患者血清IL-1β、TNF-α水平的影响,并分析其临床意义。方法对我院2014年1月—12月收治60例KOA患者临床资料及治疗效果进行回顾性分析,依据治疗方案将...目的观察透明质酸钠注射液联合塞来昔布胶囊对膝骨关节炎(knee osteoarthritis,KOA)患者血清IL-1β、TNF-α水平的影响,并分析其临床意义。方法对我院2014年1月—12月收治60例KOA患者临床资料及治疗效果进行回顾性分析,依据治疗方案将其分成对照组和研究组各30例,均给予塞来昔布胶囊、膝关节锻炼等常规治疗,研究组同时给予注射透明质酸钠注射液治疗,治疗期间均暂停止痛、激素类等药物治疗。采用酶联免疫吸附法检测治疗前后两组患者的血清IL-1β和TNF-α水平,根据KOA严重性指数(index of severity of osteoarthritis,ISOA)对患者治疗前后进行评分,并观察和比较两组不良反应发生情况。结果治疗后研究组ISOA评分较对照组明显降低(P<0.01);治疗后研究组血清IL-1β和TNF-α水平较治疗前明显减少(P<0.01);研究组不良反应发生率显著低于对照组(P<0.05)。结论透明质酸钠注射液联合塞来昔布胶囊治疗能够对KOA炎症有明显的抑制作用,缓解病情。展开更多
The fermented Chinese formula Shuan-Tong-Ling is composed of radix puerariae（Gegen）,salvia miltiorrhiza（Danshen）,radix curcuma（Jianghuang）,hawthorn（Shanzha）,salvia chinensis（Shijianchuan）,sinapis alba（Baiji...The fermented Chinese formula Shuan-Tong-Ling is composed of radix puerariae（Gegen）,salvia miltiorrhiza（Danshen）,radix curcuma（Jianghuang）,hawthorn（Shanzha）,salvia chinensis（Shijianchuan）,sinapis alba（Baijiezi）,astragalus（Huangqi）,panax japonicas（Zhujieshen）,atractylodes macrocephala koidz（Baizhu）,radix paeoniae alba（Baishao）,bupleurum（Chaihu）,chrysanthemum（Juhua）,rhizoma cyperi（Xiangfu） and gastrodin（Tianma）,whose aqueous extract was fermented with lactobacillus,bacillus aceticus and saccharomycetes.ShuanTong-Ling is a formula used to treat brain diseases including ischemic stroke,migraine,and vascular dementia.Shuan-Tong-Ling attenuated H_2O_2-induced oxidative stress in rat microvascular endothelial cells.However,the potential mechanism involved in these effects is poorly understood.Rats were intragastrically treated with 5.7 or 17.2 m L/kg Shuan-Tong-Ling for 7 days before middle cerebral artery occlusion was induced.The results indicated Shuan-Tong-Ling had a cerebral protective effect by reducing infarct volume and increasing neurological scores.Shuan-Tong-Ling also decreased tumor necrosis factor-α and interleukin-1β levels in the hippocampus on the ischemic side.In addition,Shuan-Tong-Ling upregulated the expression of SIRT1 and Bcl-2 and downregulated the expression of acetylated-protein 53 and Bax.Injection of 5 mg/kg silent information regulator 1（SIRT1） inhibitor EX527 into the subarachnoid space once every 2 days,four times,reversed the above changes.These results demonstrate that Shuan-Tong-Ling might benefit cerebral ischemia/reperfusion injury by reducing inflammation and apoptosis through activation of the SIRT1 signaling pathway.展开更多
文摘目的观察透明质酸钠注射液联合塞来昔布胶囊对膝骨关节炎(knee osteoarthritis,KOA)患者血清IL-1β、TNF-α水平的影响,并分析其临床意义。方法对我院2014年1月—12月收治60例KOA患者临床资料及治疗效果进行回顾性分析,依据治疗方案将其分成对照组和研究组各30例,均给予塞来昔布胶囊、膝关节锻炼等常规治疗,研究组同时给予注射透明质酸钠注射液治疗,治疗期间均暂停止痛、激素类等药物治疗。采用酶联免疫吸附法检测治疗前后两组患者的血清IL-1β和TNF-α水平,根据KOA严重性指数(index of severity of osteoarthritis,ISOA)对患者治疗前后进行评分,并观察和比较两组不良反应发生情况。结果治疗后研究组ISOA评分较对照组明显降低(P<0.01);治疗后研究组血清IL-1β和TNF-α水平较治疗前明显减少(P<0.01);研究组不良反应发生率显著低于对照组(P<0.05)。结论透明质酸钠注射液联合塞来昔布胶囊治疗能够对KOA炎症有明显的抑制作用,缓解病情。
基金supported by the National Natural Science Foundation of China,No.81202625Open Fund of Key Laboratory of Cardiovascular and Cerebrovascular Diseases Translational Medicine of China Three Gorges University of China,No.2016xnxg101
文摘The fermented Chinese formula Shuan-Tong-Ling is composed of radix puerariae（Gegen）,salvia miltiorrhiza（Danshen）,radix curcuma（Jianghuang）,hawthorn（Shanzha）,salvia chinensis（Shijianchuan）,sinapis alba（Baijiezi）,astragalus（Huangqi）,panax japonicas（Zhujieshen）,atractylodes macrocephala koidz（Baizhu）,radix paeoniae alba（Baishao）,bupleurum（Chaihu）,chrysanthemum（Juhua）,rhizoma cyperi（Xiangfu） and gastrodin（Tianma）,whose aqueous extract was fermented with lactobacillus,bacillus aceticus and saccharomycetes.ShuanTong-Ling is a formula used to treat brain diseases including ischemic stroke,migraine,and vascular dementia.Shuan-Tong-Ling attenuated H_2O_2-induced oxidative stress in rat microvascular endothelial cells.However,the potential mechanism involved in these effects is poorly understood.Rats were intragastrically treated with 5.7 or 17.2 m L/kg Shuan-Tong-Ling for 7 days before middle cerebral artery occlusion was induced.The results indicated Shuan-Tong-Ling had a cerebral protective effect by reducing infarct volume and increasing neurological scores.Shuan-Tong-Ling also decreased tumor necrosis factor-α and interleukin-1β levels in the hippocampus on the ischemic side.In addition,Shuan-Tong-Ling upregulated the expression of SIRT1 and Bcl-2 and downregulated the expression of acetylated-protein 53 and Bax.Injection of 5 mg/kg silent information regulator 1（SIRT1） inhibitor EX527 into the subarachnoid space once every 2 days,four times,reversed the above changes.These results demonstrate that Shuan-Tong-Ling might benefit cerebral ischemia/reperfusion injury by reducing inflammation and apoptosis through activation of the SIRT1 signaling pathway.
