目的研究肝癌细胞系HepG2中乙型肝炎表面抗原大蛋白（hepatitis B virus large surface protein，LHBs）与C53蛋白是否存在共定位，查明LHBs与C53蛋白的结合区域。方法分别构建pEGFP-C1·LHBs和pDsRedl-N1·C53质粒，共同转染...目的研究肝癌细胞系HepG2中乙型肝炎表面抗原大蛋白（hepatitis B virus large surface protein，LHBs）与C53蛋白是否存在共定位，查明LHBs与C53蛋白的结合区域。方法分别构建pEGFP-C1·LHBs和pDsRedl-N1·C53质粒，共同转染肝癌细胞系HepG2，激光共聚焦观察它们在细胞内的共定位。构建质粒pCDNA-3．1（-）myc／his-LHBs和pCMV-5a-C53，并将C53基因截短构建质粒pCMV-5a-C53N、pCMV-5a-C1、pCMV-5a-C2和pCMV．5a-C3，将pCDNA-3．1（-）myc／his．LHBs和pc-MV-5a-C53及各截短质粒共转染HepG2细胞，免疫共沉淀方法验证LHBs结合的C53区域。结果成功构建pEGFP-C1-LHBs、pDsRedl-N1-C53、pCDNA-3．1（-）myc／his-LHBs、pCMV-5a-C53、pCMV-5a-C53N、pCMV-5a-C1、pCMV-5a-C2和pCMV-5a-C3质粒；用激光共聚焦的方法证实了LHBs与C53在HepG2细胞内存在共定位；免疫共沉淀方法证实了在HepG2细胞内LHBs与C53的结合区域为其c1区。结论LHBs与C53在肝癌细胞系HepG2内存在明确的相互作用，但仍需做进一步的功能研究。展开更多
AIM To determine the variability/conservation of the domain of hepatitis B virus(HBV) pre S1 region that interacts with sodium-taurocholate cotransporting polypeptide(hereafter, NTCP-interacting domain) and the preval...AIM To determine the variability/conservation of the domain of hepatitis B virus(HBV) pre S1 region that interacts with sodium-taurocholate cotransporting polypeptide(hereafter, NTCP-interacting domain) and the prevalence of the rs2296651 polymorphism(S267 F, NTCP variant) in a Spanish population. METHODS Serum samples from 246 individuals were included and divided into 3 groups: patients with chronic HBV infection(CHB)(n = 41, 73% Caucasians), patients with resolved HBV infection(n = 100, 100% Caucasians) and an HBV-uninfected control group(n = 105, 100% Caucasians). Variability/conservation of the amino acid(aa) sequences of the NTCPinteracting domain,(aa 2-48 in viral genotype D) and a highly conserved pre S1 domain associated with virion morphogenesis(aa 92-103 in viral genotype D) were analyzed by next-generation sequencing and compared in 18 CHB patients with viremia > 4 log IU/mL. The rs2296651 polymorphism was determined in all individuals in all 3 groups using an in-house real-time PCR melting curve analysis.RESULTS The HBV pre S1 NTCP-interacting domain showed a high degree of conservation among the examined viral genomes especially between aa 9 and 21(in the genotype D consensus sequence). As compared with the virion morphogenesis domain, the NTCPinteracting domain had a smaller proportion of HBV genotype-unrelated changes comprising > 1% of the quasispecies(25.5% vs 31.8%), but a larger proportion of genotype-associated viral polymorphisms(34% vs 27.3%), according to consensus sequences from Gen Bank patterns of HBV genotypes A to H. Variation/conservation in both domains depended on viral genotype, with genotype C being the most highly conserved and genotype E the most variable(limited finding, only 2 genotype E included). Of note, proline residues were highly conserved in both domains, and serine residues showed changes only to threonine or tyrosine in the virion morphogenesis domain. The rs2296651 polymorphism was not detected in any participant.CONCLUSION In our CHB population, the NTCP-interactin展开更多
The base sequence in genome was governed by some fundamental principles such as reverse-complement symmetry, multiple fractality and so on, and the analytical method of the genome structure, the “Sequence Spectrum Me...The base sequence in genome was governed by some fundamental principles such as reverse-complement symmetry, multiple fractality and so on, and the analytical method of the genome structure, the “Sequence Spectrum Method (SSM)”, based on the structural features of genomic DNA faithfully visualized these principles. This paper reported that the sequence spectrum in SSM closely reflected the biological phenomena of protein and DNA, and SSM could identify the interactive region of protein-protein and DNA-protein uniformly. In order to investigate the effectiveness of SSM we analyzed the several protein-protein and DNA-protein interaction published primarily in the genome of Saccharomyces cerevisiae. The method proposed here was based on the homology of sequence spectrum, and it advantageously and surprisingly used only base sequence of genome and did not require any other information, even information about the amino-acid sequence of protein. Eventually it was concluded that the fundamental principles in genome governed not only the static base sequence but also the dynamic function of protein and DNA.展开更多
N-body numerical simulation is an important tool in astronomy.Scientists used this method to simulate the formation of structure of the universe,which is key to understanding how the universe formed.As research on thi...N-body numerical simulation is an important tool in astronomy.Scientists used this method to simulate the formation of structure of the universe,which is key to understanding how the universe formed.As research on this subject further develops,astronomers require a more precise method that enables expansion of the simulation and an increase in the number of simulation particles.However,retaining all temporal information is infeasible due to a lack of computer storage.In the circumstances,astronomers reserve temporal data at intervals,merging rough and baffling animations of universal evolution.In this study,we propose a deep-learning-assisted interpolation application to analyze the structure formation of the universe.First,we evaluate the feasibility of applying interpolation to generate an animation of the universal evolution through an experiment.Then,we demonstrate the superiority of deep convolutional neural network(DCNN)method by comparing its quality and performance with the actual results together with the results generated by other popular interpolation algorithms.In addition,we present PRSVis,an interactive visual analytics system that supports global volume rendering,local area magnification,and temporal animation generation.PRSVis allows users to visualize a global volume rendering,interactively select one cubic region from the rendering and intelligently produce a time-series animation of the high-resolution region using the deep-learning-assisted method.In summary,we propose an interactive visual system,integrated with the DCNN interpolation method that is validated through experiments,to help scientists easily understand the evolution of the particle region structure.展开更多
在亚洲地区,不同流域的海岸带所接受的淡水、沉积物、营养物质和污染物质入海通量有着很大的差异。入海通量的特征受到流域人类活动的强烈影响,如水坝建设和化肥的大量施用。气候和海平面的变化也起了一定的作用。流域—海岸相互作用的...在亚洲地区,不同流域的海岸带所接受的淡水、沉积物、营养物质和污染物质入海通量有着很大的差异。入海通量的特征受到流域人类活动的强烈影响,如水坝建设和化肥的大量施用。气候和海平面的变化也起了一定的作用。流域—海岸相互作用的特点是,入海物质通量的变化将导致流域、河口以及邻近水域的地貌、环境和生态系统的改变。在流域—海岸系统不断变化的情况下,为了改进流域—海岸系统开发的管理策略,应进行以下调查和研究工作:①在现场监测和观察的基础上,定量地描述上述变化;②了解引起上述变化的过程和机制;③发展预测未来变化的趋势和幅度的新方法、新技术;④将所获结果应用于流域—海岸系统的开发和管理实践。为了确定本研究领域今后10年的资助方向,APN(Asian Pacific Network for G lobal Change Research)召集了相关的学术研讨会。按照APN的部署,笔者负责完成了“流域—海岸相互作用”领域的研究课题建议,本文是这份文件的简要总结。展开更多
文摘目的研究肝癌细胞系HepG2中乙型肝炎表面抗原大蛋白（hepatitis B virus large surface protein，LHBs）与C53蛋白是否存在共定位，查明LHBs与C53蛋白的结合区域。方法分别构建pEGFP-C1·LHBs和pDsRedl-N1·C53质粒，共同转染肝癌细胞系HepG2，激光共聚焦观察它们在细胞内的共定位。构建质粒pCDNA-3．1（-）myc／his-LHBs和pCMV-5a-C53，并将C53基因截短构建质粒pCMV-5a-C53N、pCMV-5a-C1、pCMV-5a-C2和pCMV．5a-C3，将pCDNA-3．1（-）myc／his．LHBs和pc-MV-5a-C53及各截短质粒共转染HepG2细胞，免疫共沉淀方法验证LHBs结合的C53区域。结果成功构建pEGFP-C1-LHBs、pDsRedl-N1-C53、pCDNA-3．1（-）myc／his-LHBs、pCMV-5a-C53、pCMV-5a-C53N、pCMV-5a-C1、pCMV-5a-C2和pCMV-5a-C3质粒；用激光共聚焦的方法证实了LHBs与C53在HepG2细胞内存在共定位；免疫共沉淀方法证实了在HepG2细胞内LHBs与C53的结合区域为其c1区。结论LHBs与C53在肝癌细胞系HepG2内存在明确的相互作用，但仍需做进一步的功能研究。
基金Supported by Instituto de Salud Carlos Ⅲ,No.PI14/01416 and No.PI15/00856cofinanced by the European Regional Development Fund(ERDF)the Gilead Fellowship Program,No.GLD14-00296
文摘AIM To determine the variability/conservation of the domain of hepatitis B virus(HBV) pre S1 region that interacts with sodium-taurocholate cotransporting polypeptide(hereafter, NTCP-interacting domain) and the prevalence of the rs2296651 polymorphism(S267 F, NTCP variant) in a Spanish population. METHODS Serum samples from 246 individuals were included and divided into 3 groups: patients with chronic HBV infection(CHB)(n = 41, 73% Caucasians), patients with resolved HBV infection(n = 100, 100% Caucasians) and an HBV-uninfected control group(n = 105, 100% Caucasians). Variability/conservation of the amino acid(aa) sequences of the NTCPinteracting domain,(aa 2-48 in viral genotype D) and a highly conserved pre S1 domain associated with virion morphogenesis(aa 92-103 in viral genotype D) were analyzed by next-generation sequencing and compared in 18 CHB patients with viremia > 4 log IU/mL. The rs2296651 polymorphism was determined in all individuals in all 3 groups using an in-house real-time PCR melting curve analysis.RESULTS The HBV pre S1 NTCP-interacting domain showed a high degree of conservation among the examined viral genomes especially between aa 9 and 21(in the genotype D consensus sequence). As compared with the virion morphogenesis domain, the NTCPinteracting domain had a smaller proportion of HBV genotype-unrelated changes comprising > 1% of the quasispecies(25.5% vs 31.8%), but a larger proportion of genotype-associated viral polymorphisms(34% vs 27.3%), according to consensus sequences from Gen Bank patterns of HBV genotypes A to H. Variation/conservation in both domains depended on viral genotype, with genotype C being the most highly conserved and genotype E the most variable(limited finding, only 2 genotype E included). Of note, proline residues were highly conserved in both domains, and serine residues showed changes only to threonine or tyrosine in the virion morphogenesis domain. The rs2296651 polymorphism was not detected in any participant.CONCLUSION In our CHB population, the NTCP-interactin
文摘The base sequence in genome was governed by some fundamental principles such as reverse-complement symmetry, multiple fractality and so on, and the analytical method of the genome structure, the “Sequence Spectrum Method (SSM)”, based on the structural features of genomic DNA faithfully visualized these principles. This paper reported that the sequence spectrum in SSM closely reflected the biological phenomena of protein and DNA, and SSM could identify the interactive region of protein-protein and DNA-protein uniformly. In order to investigate the effectiveness of SSM we analyzed the several protein-protein and DNA-protein interaction published primarily in the genome of Saccharomyces cerevisiae. The method proposed here was based on the homology of sequence spectrum, and it advantageously and surprisingly used only base sequence of genome and did not require any other information, even information about the amino-acid sequence of protein. Eventually it was concluded that the fundamental principles in genome governed not only the static base sequence but also the dynamic function of protein and DNA.
基金This work is supported by the National Key Research and Development Program of China(2020YFB0204802).
文摘N-body numerical simulation is an important tool in astronomy.Scientists used this method to simulate the formation of structure of the universe,which is key to understanding how the universe formed.As research on this subject further develops,astronomers require a more precise method that enables expansion of the simulation and an increase in the number of simulation particles.However,retaining all temporal information is infeasible due to a lack of computer storage.In the circumstances,astronomers reserve temporal data at intervals,merging rough and baffling animations of universal evolution.In this study,we propose a deep-learning-assisted interpolation application to analyze the structure formation of the universe.First,we evaluate the feasibility of applying interpolation to generate an animation of the universal evolution through an experiment.Then,we demonstrate the superiority of deep convolutional neural network(DCNN)method by comparing its quality and performance with the actual results together with the results generated by other popular interpolation algorithms.In addition,we present PRSVis,an interactive visual analytics system that supports global volume rendering,local area magnification,and temporal animation generation.PRSVis allows users to visualize a global volume rendering,interactively select one cubic region from the rendering and intelligently produce a time-series animation of the high-resolution region using the deep-learning-assisted method.In summary,we propose an interactive visual system,integrated with the DCNN interpolation method that is validated through experiments,to help scientists easily understand the evolution of the particle region structure.
文摘在亚洲地区,不同流域的海岸带所接受的淡水、沉积物、营养物质和污染物质入海通量有着很大的差异。入海通量的特征受到流域人类活动的强烈影响,如水坝建设和化肥的大量施用。气候和海平面的变化也起了一定的作用。流域—海岸相互作用的特点是,入海物质通量的变化将导致流域、河口以及邻近水域的地貌、环境和生态系统的改变。在流域—海岸系统不断变化的情况下,为了改进流域—海岸系统开发的管理策略,应进行以下调查和研究工作:①在现场监测和观察的基础上,定量地描述上述变化;②了解引起上述变化的过程和机制;③发展预测未来变化的趋势和幅度的新方法、新技术;④将所获结果应用于流域—海岸系统的开发和管理实践。为了确定本研究领域今后10年的资助方向,APN(Asian Pacific Network for G lobal Change Research)召集了相关的学术研讨会。按照APN的部署,笔者负责完成了“流域—海岸相互作用”领域的研究课题建议,本文是这份文件的简要总结。
