研究三维空间机器人路径规划问题,由于系统求解时间较长、过早失去解的多样性、易陷入局部最优、个体适应度较差等问题,通过构建三维工作空间模型、引入变异算子和搜索无碰路径策略来解决,提出适宜于三维机器人路径规划的一种变异算子...研究三维空间机器人路径规划问题,由于系统求解时间较长、过早失去解的多样性、易陷入局部最优、个体适应度较差等问题,通过构建三维工作空间模型、引入变异算子和搜索无碰路径策略来解决,提出适宜于三维机器人路径规划的一种变异算子蚁群算法(Mutation Operator Ant Colony Algorithm,MOACA)。MOACA是一种关于模型构造的启发式搜索算法,算法在改进启发式函数设计、选择概率确定、信息素更新策略等基础上,引入逆转变异和插入变异算子,通过选择逆转点反序排列部分路径节点和随机插入路径节点的方法搜索无碰路径,对蚁群算法进行了局部优化改良。仿真结果表明,MOACA在搜索路径、收敛时间、适应度等方面较传统蚁群算法有明显改善,算法是有效可行的。展开更多
Genetic algorithms(GAs)are very good metaheuristic algorithms that are suitable for solving NP-hard combinatorial optimization problems.AsimpleGAbeginswith a set of solutions represented by a population of chromosomes...Genetic algorithms(GAs)are very good metaheuristic algorithms that are suitable for solving NP-hard combinatorial optimization problems.AsimpleGAbeginswith a set of solutions represented by a population of chromosomes and then uses the idea of survival of the fittest in the selection process to select some fitter chromosomes.It uses a crossover operator to create better offspring chromosomes and thus,converges the population.Also,it uses a mutation operator to explore the unexplored areas by the crossover operator,and thus,diversifies the GA search space.A combination of crossover and mutation operators makes the GA search strong enough to reach the optimal solution.However,appropriate selection and combination of crossover operator and mutation operator can lead to a very good GA for solving an optimization problem.In this present paper,we aim to study the benchmark traveling salesman problem(TSP).We developed several genetic algorithms using seven crossover operators and six mutation operators for the TSP and then compared them to some benchmark TSPLIB instances.The experimental studies show the effectiveness of the combination of a comprehensive sequential constructive crossover operator and insertion mutation operator for the problem.The GA using the comprehensive sequential constructive crossover with insertion mutation could find average solutions whose average percentage of excesses from the best-known solutions are between 0.22 and 14.94 for our experimented problem instances.展开更多
目的迟发性脊柱骨骺发育不良(spondyloepiphyseal dysplasia tarda,SEDT)是一种X线表现明显的X连锁骨发育不良疾病,主要特征包括不成比例的短躯干型矮身材、大关节发育不良以及胸腰椎椎体扁平。文中鉴定SEDT家系SEDL基因的突变位点,探讨...目的迟发性脊柱骨骺发育不良(spondyloepiphyseal dysplasia tarda,SEDT)是一种X线表现明显的X连锁骨发育不良疾病,主要特征包括不成比例的短躯干型矮身材、大关节发育不良以及胸腰椎椎体扁平。文中鉴定SEDT家系SEDL基因的突变位点,探讨SEDT发病的分子遗传学机制。方法报告1个4代68人累及8例患者的SEDT大家系,患者均为非匀称性矮身材(短躯干),临床诊断为X连锁SEDT。提取家系所有成员及50例正常对照者血样中白细胞基因组DNA,应用PCR和DNA测序进行SEDL基因突变分析。结果DNA序列分析表明,家系8例患者均为SEDL基因外显子6发生插入突变(c.370-371 ins A,突变数据库检索未见报告,为一全新突变方式),并发现1例无症状患儿携带相同方式突变(症状发生前),6例女性携带者为杂合突变,家系其他成员和正常对照者中均未见该插入突变。结论SEDL基因c.370-371 ins A是一新突变方式,详细的临床与分子遗传学研究丰富了SEDT的基因型-表型谱。SEDL基因突变分析对携带者检测、症状前诊断以及患者和携带者的产前基因诊断具有重要意义。展开更多
EmpA is an extracellular metalloprotease secreted by Vibrio anguillarum.For better understanding its role in the patho-genicity of V.anguillarum strain M3,empA insertion mutant was constructed.In the mutant it decreas...EmpA is an extracellular metalloprotease secreted by Vibrio anguillarum.For better understanding its role in the patho-genicity of V.anguillarum strain M3,empA insertion mutant was constructed.In the mutant it decreased in extracellular proteolytic activity,swarming motility,hemolytic activity and virulence on turbot(Scophthalmus maximus).Significant decline(by 5-fold)of extracellular proteolytic activity and similar growth curve between mutant and wild type strains indicated that EmpA was the major extracellular protease of M3.LD50 of mutant increased by 38-fold compared with wild type.No pro-EmpA was detected in the su-pernatant of culture,indicating that EmpA autolyzed to mature protein after 24 h.Secretion of EmpA in M3 was similar to that in NB10 strain.Attenuated virulence of mutant was similar to that of M93Sm strain.It was demonstrated that specific operation of EmpA was different from that in previous studies and EmpA contributed to the swarming motility and hemolytic activity in V.an-guillarum strain M3.The results provides insight into understanding the function of EmpA and its potential application in vaccine development.展开更多
[ Objective] The aim of this study is to obtain transgenic Lilium longiflorum Thumb. [ Method] A two-step method of explant and the T-DNA integration technique were employed to transform Lilium longiflorum via Agrobac...[ Objective] The aim of this study is to obtain transgenic Lilium longiflorum Thumb. [ Method] A two-step method of explant and the T-DNA integration technique were employed to transform Lilium longiflorum via Agrobacterium mediated method. [ Result] The best infection effect appeared under the OD600 value of Agrobacterium within 0.6 -0.8, the addition of 250 mg/L AS could increase the transformation efficiency. The optimal concentration of G418 for screening is 50 mg/L. Some putative transgenic plants of Lilium longiflorum with resistance to G418 showed positive in PCR, preliminarily proving that T-DNA gene had integrated into the genome of lily. [ Conclusion] The study may lay a foundation for breeding excellent lily varieties through TDNA integration technique.展开更多
A rice heading-date-related mutant was isolated from a ^60Co-y-ray-induced mutation pool of Zhejing 22, a conventional japonica cultivar in Zhejiang Province, China. The mutant was characterized by a delayed heading d...A rice heading-date-related mutant was isolated from a ^60Co-y-ray-induced mutation pool of Zhejing 22, a conventional japonica cultivar in Zhejiang Province, China. The mutant was characterized by a delayed heading date of almost 20 d longer than the wild type plant. Genetic analysis revealed that the mutation was controlled by a single nuclear-encoded recessive gene that was designed as HD（t） （heading date tentatively）. To isolate the HD（t） gene, a map-based cloning approach was employed using 479 F2 mutant individual plants derived from the cross between the hd（t） mutant （japonica） x Zhenshan 97 （indica）. Finally, the HD（t） gene was mapped to an approximate 53 kb region between the insertion and deletion （InDel） markers of 10-61W and 10-66W on chromosome 10. According to the genome sequence of Nipponbare, the target region contains 11 annotated genes. It is helpful for future cloning of HD（t） gene based on this fine mapping results.展开更多
文摘研究三维空间机器人路径规划问题,由于系统求解时间较长、过早失去解的多样性、易陷入局部最优、个体适应度较差等问题,通过构建三维工作空间模型、引入变异算子和搜索无碰路径策略来解决,提出适宜于三维机器人路径规划的一种变异算子蚁群算法(Mutation Operator Ant Colony Algorithm,MOACA)。MOACA是一种关于模型构造的启发式搜索算法,算法在改进启发式函数设计、选择概率确定、信息素更新策略等基础上,引入逆转变异和插入变异算子,通过选择逆转点反序排列部分路径节点和随机插入路径节点的方法搜索无碰路径,对蚁群算法进行了局部优化改良。仿真结果表明,MOACA在搜索路径、收敛时间、适应度等方面较传统蚁群算法有明显改善,算法是有效可行的。
基金the Deanship of Scientific Research at Imam Mohammad Ibn Saud Islamic University(IMSIU)(Grant Number IMSIU-RP23030).
文摘Genetic algorithms(GAs)are very good metaheuristic algorithms that are suitable for solving NP-hard combinatorial optimization problems.AsimpleGAbeginswith a set of solutions represented by a population of chromosomes and then uses the idea of survival of the fittest in the selection process to select some fitter chromosomes.It uses a crossover operator to create better offspring chromosomes and thus,converges the population.Also,it uses a mutation operator to explore the unexplored areas by the crossover operator,and thus,diversifies the GA search space.A combination of crossover and mutation operators makes the GA search strong enough to reach the optimal solution.However,appropriate selection and combination of crossover operator and mutation operator can lead to a very good GA for solving an optimization problem.In this present paper,we aim to study the benchmark traveling salesman problem(TSP).We developed several genetic algorithms using seven crossover operators and six mutation operators for the TSP and then compared them to some benchmark TSPLIB instances.The experimental studies show the effectiveness of the combination of a comprehensive sequential constructive crossover operator and insertion mutation operator for the problem.The GA using the comprehensive sequential constructive crossover with insertion mutation could find average solutions whose average percentage of excesses from the best-known solutions are between 0.22 and 14.94 for our experimented problem instances.
文摘目的迟发性脊柱骨骺发育不良(spondyloepiphyseal dysplasia tarda,SEDT)是一种X线表现明显的X连锁骨发育不良疾病,主要特征包括不成比例的短躯干型矮身材、大关节发育不良以及胸腰椎椎体扁平。文中鉴定SEDT家系SEDL基因的突变位点,探讨SEDT发病的分子遗传学机制。方法报告1个4代68人累及8例患者的SEDT大家系,患者均为非匀称性矮身材(短躯干),临床诊断为X连锁SEDT。提取家系所有成员及50例正常对照者血样中白细胞基因组DNA,应用PCR和DNA测序进行SEDL基因突变分析。结果DNA序列分析表明,家系8例患者均为SEDL基因外显子6发生插入突变(c.370-371 ins A,突变数据库检索未见报告,为一全新突变方式),并发现1例无症状患儿携带相同方式突变(症状发生前),6例女性携带者为杂合突变,家系其他成员和正常对照者中均未见该插入突变。结论SEDL基因c.370-371 ins A是一新突变方式,详细的临床与分子遗传学研究丰富了SEDT的基因型-表型谱。SEDL基因突变分析对携带者检测、症状前诊断以及患者和携带者的产前基因诊断具有重要意义。
基金This project was funded by the 973 Program (2006CB101803)the National Natural Sciences Foundation of China (30871935)
文摘EmpA is an extracellular metalloprotease secreted by Vibrio anguillarum.For better understanding its role in the patho-genicity of V.anguillarum strain M3,empA insertion mutant was constructed.In the mutant it decreased in extracellular proteolytic activity,swarming motility,hemolytic activity and virulence on turbot(Scophthalmus maximus).Significant decline(by 5-fold)of extracellular proteolytic activity and similar growth curve between mutant and wild type strains indicated that EmpA was the major extracellular protease of M3.LD50 of mutant increased by 38-fold compared with wild type.No pro-EmpA was detected in the su-pernatant of culture,indicating that EmpA autolyzed to mature protein after 24 h.Secretion of EmpA in M3 was similar to that in NB10 strain.Attenuated virulence of mutant was similar to that of M93Sm strain.It was demonstrated that specific operation of EmpA was different from that in previous studies and EmpA contributed to the swarming motility and hemolytic activity in V.an-guillarum strain M3.The results provides insight into understanding the function of EmpA and its potential application in vaccine development.
基金the Fund of Basis Scientific Research Operation of Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciencesthe Grant of Scientific Fund of Chinese Academy of Tropical Agricultural Sciences (NoRky0529)~~
文摘[ Objective] The aim of this study is to obtain transgenic Lilium longiflorum Thumb. [ Method] A two-step method of explant and the T-DNA integration technique were employed to transform Lilium longiflorum via Agrobacterium mediated method. [ Result] The best infection effect appeared under the OD600 value of Agrobacterium within 0.6 -0.8, the addition of 250 mg/L AS could increase the transformation efficiency. The optimal concentration of G418 for screening is 50 mg/L. Some putative transgenic plants of Lilium longiflorum with resistance to G418 showed positive in PCR, preliminarily proving that T-DNA gene had integrated into the genome of lily. [ Conclusion] The study may lay a foundation for breeding excellent lily varieties through TDNA integration technique.
基金supported by the Chinese Ministry of Agriculture(Grant Nos.200803034 and 2011ZX08001001)the Zhejiang Provincial and the National Natural Science Foundation of China(Grant Nos.Y3080361 and 31071207)+2 种基金the Science and Technology Department of Zhejiang Province,China(Grant No.2004C12020)the Special Fund for Agro-scientific Research in the Public Interest(Grant No.201103007)the Special Fund of Zhejiang Academy of Agricultural Science,China
文摘A rice heading-date-related mutant was isolated from a ^60Co-y-ray-induced mutation pool of Zhejing 22, a conventional japonica cultivar in Zhejiang Province, China. The mutant was characterized by a delayed heading date of almost 20 d longer than the wild type plant. Genetic analysis revealed that the mutation was controlled by a single nuclear-encoded recessive gene that was designed as HD（t） （heading date tentatively）. To isolate the HD（t） gene, a map-based cloning approach was employed using 479 F2 mutant individual plants derived from the cross between the hd（t） mutant （japonica） x Zhenshan 97 （indica）. Finally, the HD（t） gene was mapped to an approximate 53 kb region between the insertion and deletion （InDel） markers of 10-61W and 10-66W on chromosome 10. According to the genome sequence of Nipponbare, the target region contains 11 annotated genes. It is helpful for future cloning of HD（t） gene based on this fine mapping results.
