AIM： To examine light-emitting-diode（LED）-induced retinal neuronal cell damage and its wavelength-driven pathogenic mechanisms.METHODS： Sprague-Dawley rats were exposed to blue LEDs（460 nm）,green LEDs（530 nm）,...AIM： To examine light-emitting-diode（LED）-induced retinal neuronal cell damage and its wavelength-driven pathogenic mechanisms.METHODS： Sprague-Dawley rats were exposed to blue LEDs（460 nm）,green LEDs（530 nm）,and red LEDs（620 nm）.Electroretinography（ERG）,Hematoxylin and eosin（H＆E） staining,transmission electron microscopy（TEM）,terminal deoxynucleotidyl transferase d UTP nick end labeling（TUNEL）,and immunohistochemical（IHC） staining,Western blotting（WB） and the detection of superoxide anion（O2^-·）,hydrogen peroxide（H2O2）,total iron,and ferric（Fe^3＋） levels were applied.RESULTS： ERG results showed the blue LED group induced more functional damage than that of green or red LED groups.H＆E staining,TUNEL,IHC,and TEM revealed apoptosis and necrosis of photoreceptors and RPE,which indicated blue LED also induced more photochemical injury.Free radical production and iron-related molecular marker expressions demonstrated that oxidative stress and ironoverload were associated with retinal injury.WB assays correspondingly showed that defense gene expression was up-regulated after the LED light exposure with a wavelength dependency.CONCLUSION： The study results indicate that LED bluelight exposure poses a great risk of retinal injury in awake,task-oriented rod-dominant animals.The wavelengthdependent effect should be considered carefully when switching to LED lighting applications.展开更多
目的观察PPARγ在通气相关性肺损伤大鼠肺组织中的表达,探讨PPARγ在通气相关性肺损伤中的作用。方法清洁雄性SD大鼠,随机分为大潮气量(Tidal volume,VT)组,Vt=12 m L;小潮气量组,Vt=6 m L;自主呼吸组。每组又分为3个亚组:即通气1 h、4 ...目的观察PPARγ在通气相关性肺损伤大鼠肺组织中的表达,探讨PPARγ在通气相关性肺损伤中的作用。方法清洁雄性SD大鼠,随机分为大潮气量(Tidal volume,VT)组,Vt=12 m L;小潮气量组,Vt=6 m L;自主呼吸组。每组又分为3个亚组:即通气1 h、4 h、8 h组。于各时间段末放血处死动物,收集肺组织和肺灌洗液标本,测定肺灌洗液中蛋白总量、白细胞计数;测定肺组织湿/干重比(W/D);逆转录聚合酶链反应检测PPARγmRNA的表达;western blot检测PPARγ蛋白的变化。结果大潮气量组机械通气4 h、8 h后,与小潮气量组及自主呼吸组相比,肺灌洗液中白细胞计数、蛋白总量明显增加(P<0.01),肺W/D增加(P<0.01);肺组织出现明显病理组织学损伤;PPARγmRNA和PPARγ蛋白表达减少(P<0.01)。通气1 h时,各组之间比较无显著差异(P>0.05)。结论通气相关性肺损伤大鼠肺组织中PPARγ基因和蛋白表达下降,这可能与炎症损伤和持续有关。展开更多
Puerarin is a major active ingredient of the traditional Chinese plant medicine,Radix Puerariae,and commonly used in the treatment of myocardial and cerebral ischemia.However,the effects of puerarin on neuropathic pai...Puerarin is a major active ingredient of the traditional Chinese plant medicine,Radix Puerariae,and commonly used in the treatment of myocardial and cerebral ischemia.However,the effects of puerarin on neuropathic pain are still unclear.In this study,a neuropathic pain animal model was created by partial sciatic nerve ligation.Puerarin（30 or 60 mg/kg） was intraperitoneally injected once a day for 7 days.Mechanical allodynia and thermal hyperalgesia were examined at 1 day after model establishment.Mechanical threshold and paw withdrawal latency markedly increased in a dose-dependent manner in puerarin-treated rats,especially at 7 days after model establishment.At 7 days after model establishment,quantitative real-time reverse transcriptase-polymerase chain reaction results showed that puerarin administration reversed m RNA expression of transient receptor potential vanilloid 1（Trpv1） and transient receptor potential ankyrin 1（Trpa1） in a dose-dependent manner in dorsal root ganglion neurons after peripheral nerve injury.These results suggest that puerarin dose-dependently ameliorates neuropathic pain by suppressing Trpv1 and Trpa1 up-regulation in dorsal root ganglion of neuropathic pain rats.展开更多
基金Supported by Taiwan Ministry of Science and Technology grant(No.NSC 103-2314-B-002-076-MY3)
文摘AIM： To examine light-emitting-diode（LED）-induced retinal neuronal cell damage and its wavelength-driven pathogenic mechanisms.METHODS： Sprague-Dawley rats were exposed to blue LEDs（460 nm）,green LEDs（530 nm）,and red LEDs（620 nm）.Electroretinography（ERG）,Hematoxylin and eosin（H＆E） staining,transmission electron microscopy（TEM）,terminal deoxynucleotidyl transferase d UTP nick end labeling（TUNEL）,and immunohistochemical（IHC） staining,Western blotting（WB） and the detection of superoxide anion（O2^-·）,hydrogen peroxide（H2O2）,total iron,and ferric（Fe^3＋） levels were applied.RESULTS： ERG results showed the blue LED group induced more functional damage than that of green or red LED groups.H＆E staining,TUNEL,IHC,and TEM revealed apoptosis and necrosis of photoreceptors and RPE,which indicated blue LED also induced more photochemical injury.Free radical production and iron-related molecular marker expressions demonstrated that oxidative stress and ironoverload were associated with retinal injury.WB assays correspondingly showed that defense gene expression was up-regulated after the LED light exposure with a wavelength dependency.CONCLUSION： The study results indicate that LED bluelight exposure poses a great risk of retinal injury in awake,task-oriented rod-dominant animals.The wavelengthdependent effect should be considered carefully when switching to LED lighting applications.
基金supported by the National Natural Science Foundation of China,No.81671891
文摘Puerarin is a major active ingredient of the traditional Chinese plant medicine,Radix Puerariae,and commonly used in the treatment of myocardial and cerebral ischemia.However,the effects of puerarin on neuropathic pain are still unclear.In this study,a neuropathic pain animal model was created by partial sciatic nerve ligation.Puerarin（30 or 60 mg/kg） was intraperitoneally injected once a day for 7 days.Mechanical allodynia and thermal hyperalgesia were examined at 1 day after model establishment.Mechanical threshold and paw withdrawal latency markedly increased in a dose-dependent manner in puerarin-treated rats,especially at 7 days after model establishment.At 7 days after model establishment,quantitative real-time reverse transcriptase-polymerase chain reaction results showed that puerarin administration reversed m RNA expression of transient receptor potential vanilloid 1（Trpv1） and transient receptor potential ankyrin 1（Trpa1） in a dose-dependent manner in dorsal root ganglion neurons after peripheral nerve injury.These results suggest that puerarin dose-dependently ameliorates neuropathic pain by suppressing Trpv1 and Trpa1 up-regulation in dorsal root ganglion of neuropathic pain rats.
