AIM:To determine the effects of intravitreal resveratrol(RSV)on murine laser-induced choroidal neovascularization(CNV).METHODS:The toxicity of RSV to choroidal endothelial cell(CEC)was measured using thiazolyl blue te...AIM:To determine the effects of intravitreal resveratrol(RSV)on murine laser-induced choroidal neovascularization(CNV).METHODS:The toxicity of RSV to choroidal endothelial cell(CEC)was measured using thiazolyl blue tetrazolium bromide(M一)assay.Effects of RSV on choroidal endothelial cell(CEC)migration were evaluated with a modified Boyden chamber assay,while tube formation was evaluated in a 2-D gel assay.CNV was induced by laser photocoagulation in mice.The effects of intravitreal injection of RSV on CNV development were evaluated by fluorescein angiography(FA),confocal analysis of isolectin B4 labeled choroidal flat mounts,and histologic examination of CNV membranes.Immunostaining was used to analyze the expression and phosphorylation of vascular endothelial growth factor receptor 2(VEGFR2).RESULTS:No significant cell toxicity was observed in CEC if the concentration of RSV was less than 200 pmol/L(P>0.05).RSV inhibited vascular endothelial growth factor(VEGF)-induced CEC migration(P<0.05)and tube formation(P<0.05)invitro.Furthermore,intravitrealinjectionof RSV significantly inhibited laser induced CNV formation in mice.The FA leakage,CNV volume and CNV area analysis revealed that there were 41%,45%,and 58%reduction in RSV-treated eyes(1.691±0.1032,178163±78623μm^3 and 6508±619.0μm^2,respectively)compared with those in control(2.724±0.08447,379676±98382μm3and16576±2646μm^2,respectively;P<0.05).Phospho-VEGFR2expression was much weaker in the sections of CNV lesions in RSV injected mice compared with that in control(P<0.05).CONCLUSION:Intravitreal injection of RSV exerts an inhibitory effect on CNV,which may through suppressing endothelial cell migration,tube formation and VEGFR2 phosphorylation.展开更多
A polyethylene tube can be used as a heat exchanger for a low-running-cost?temperature control system. In this system, the flow of temperature-controlled?water in the tube is used as the heat source, and the tube is p...A polyethylene tube can be used as a heat exchanger for a low-running-cost?temperature control system. In this system, the flow of temperature-controlled?water in the tube is used as the heat source, and the tube is placed on the ceiling of a temperature-controlled space using a metal net. Owing to this structure, the tube is deformed by its weight. This deformation has a significant influence on heat transfer and flow characteristics. Therefore, an air injection method, in which air and water are injected simultaneously into the tube, is developed for preventing the deformation of the tube. In this study, bedding metal rods were used instead of a metal net. The influence of the pitch length of the metal rods (5 - 15 cm) and the width of the polyethylene tube 15, 20, 25, 30, and 35 cm was examined experimentally. The length of the polyethylene tube was 178 cm. The air flow rate was 9.5 × 10-5 m3/s. The water flow rates were 60, 80, 100, 120, and 140 mL/min. Results show that the thermal response improved because of air injection. In particular, the temperature at steady state increased, and steady state was attained approximately 1.2 - 3 times faster with air injection than without air injection. The optimum pitch length of the metal rods and the range of the optimum width of the polyethylene tube were 8 cm and 20 - 25 cm, respectively, with and without air injection.展开更多
基金Supported by the National Natural Science Foundation of China(No.81703134,No.81770952)Henan Province Nature Science Foundation of China(No.162300410296)Hunan Province Nature Science Foundation of China(No.2018JJ3772)。
文摘AIM:To determine the effects of intravitreal resveratrol(RSV)on murine laser-induced choroidal neovascularization(CNV).METHODS:The toxicity of RSV to choroidal endothelial cell(CEC)was measured using thiazolyl blue tetrazolium bromide(M一)assay.Effects of RSV on choroidal endothelial cell(CEC)migration were evaluated with a modified Boyden chamber assay,while tube formation was evaluated in a 2-D gel assay.CNV was induced by laser photocoagulation in mice.The effects of intravitreal injection of RSV on CNV development were evaluated by fluorescein angiography(FA),confocal analysis of isolectin B4 labeled choroidal flat mounts,and histologic examination of CNV membranes.Immunostaining was used to analyze the expression and phosphorylation of vascular endothelial growth factor receptor 2(VEGFR2).RESULTS:No significant cell toxicity was observed in CEC if the concentration of RSV was less than 200 pmol/L(P>0.05).RSV inhibited vascular endothelial growth factor(VEGF)-induced CEC migration(P<0.05)and tube formation(P<0.05)invitro.Furthermore,intravitrealinjectionof RSV significantly inhibited laser induced CNV formation in mice.The FA leakage,CNV volume and CNV area analysis revealed that there were 41%,45%,and 58%reduction in RSV-treated eyes(1.691±0.1032,178163±78623μm^3 and 6508±619.0μm^2,respectively)compared with those in control(2.724±0.08447,379676±98382μm3and16576±2646μm^2,respectively;P<0.05).Phospho-VEGFR2expression was much weaker in the sections of CNV lesions in RSV injected mice compared with that in control(P<0.05).CONCLUSION:Intravitreal injection of RSV exerts an inhibitory effect on CNV,which may through suppressing endothelial cell migration,tube formation and VEGFR2 phosphorylation.
文摘A polyethylene tube can be used as a heat exchanger for a low-running-cost?temperature control system. In this system, the flow of temperature-controlled?water in the tube is used as the heat source, and the tube is placed on the ceiling of a temperature-controlled space using a metal net. Owing to this structure, the tube is deformed by its weight. This deformation has a significant influence on heat transfer and flow characteristics. Therefore, an air injection method, in which air and water are injected simultaneously into the tube, is developed for preventing the deformation of the tube. In this study, bedding metal rods were used instead of a metal net. The influence of the pitch length of the metal rods (5 - 15 cm) and the width of the polyethylene tube 15, 20, 25, 30, and 35 cm was examined experimentally. The length of the polyethylene tube was 178 cm. The air flow rate was 9.5 × 10-5 m3/s. The water flow rates were 60, 80, 100, 120, and 140 mL/min. Results show that the thermal response improved because of air injection. In particular, the temperature at steady state increased, and steady state was attained approximately 1.2 - 3 times faster with air injection than without air injection. The optimum pitch length of the metal rods and the range of the optimum width of the polyethylene tube were 8 cm and 20 - 25 cm, respectively, with and without air injection.
