The second near-infrared(NIR-Ⅱ,1000-1700 nm)window provides a superior optical platform with high resolution,deep penetration and high signal-to-noise ratios(SNRs),which results from the intrinsic low scattering and ...The second near-infrared(NIR-Ⅱ,1000-1700 nm)window provides a superior optical platform with high resolution,deep penetration and high signal-to-noise ratios(SNRs),which results from the intrinsic low scattering and auto fluorescence in biological tissues.As one of the promising NIR-Ⅱemitting probes,lanthanide based nanoparticles(LnNPs)exhibit high photo stability and chemostability,long photoluminescence lifetimes,low long-term cytotoxicity and narrow emission bandwidths.All these merits have spurred the evolution of related bio-optics and a variety of biomedical applications of LnNPs.This mini-review discusses the most recent advances in both the design-the composition and surface modifications-and the applications of NIR-Ⅱemitting LnNPs in bioimaging,disease diagnosis and therapy.We also summarize the current limits and challenges facing the applications of LnNPs as well as discuss the directions of future development.展开更多
AIM: To investigate feasibility and accuracy of near-infrared fluorescence imaging using indocyanine green: nanocolloid for sentinel lymph node (SLN) detection in gastric cancer.METHODS: A prospective, single-institut...AIM: To investigate feasibility and accuracy of near-infrared fluorescence imaging using indocyanine green: nanocolloid for sentinel lymph node (SLN) detection in gastric cancer.METHODS: A prospective, single-institution, phase I feasibility trial was conducted. Patients suffering from gastric cancer and planned for gastrectomy were included. During surgery, a subserosal injection of 1.6 mL ICG:Nanocoll was administered around the tumor. NIR fluorescence imaging of the abdominal cavity was performed using the Mini-FLARE™ NIR fluorescence imaging system. Lymphatic pathways and SLNs were visualized. Of every detected SLN, the corresponding lymph node station, signal-to-background ratio and histopathological diagnosis was determined. Patients underwent standard-of-care gastrectomy. Detected SLNs outside the standard dissection planes were also resected and evaluated.RESULTS: Twenty-six patients were enrolled. Four patients were excluded because distant metastases were found during surgery or due to technical failure of the injection. In 21 of the remaining 22 patients, at least 1 SLN was detected by NIR Fluorescence imaging (mean 3.1 SLNs; range 1-6). In 8 of the 21 patients, tumor-positive LNs were found. Overall accuracy of the technique was 90% (70%-99%; 95%CI), which decreased by higher pT-stage (100%, 100%, 100%, 90%, 0% for respectively Tx, T1, T2, T3, T4 tumors). All NIR-negative SLNs were completely effaced by tumor. Mean fluorescence signal-to-background ratio of SLNs was 4.4 (range 1.4-19.8). In 8 of the 21 patients, SLNs outside the standard resection plane were identified, that contained malignant cells in 2 patients.CONCLUSION: This study shows successful use of ICG:Nanocoll as lymphatic tracer for SLN detection in gastric cancer. Moreover, tumor-containing LNs outside the standard dissection planes were identified.展开更多
Aluminum flocculant can enhance the flocculating performance of activated sludge.However,the binding mechanism of aluminum ion（Al 3＋） and extracellular polymeric substances（EPS） in activated sludge is unclear due...Aluminum flocculant can enhance the flocculating performance of activated sludge.However,the binding mechanism of aluminum ion（Al 3＋） and extracellular polymeric substances（EPS） in activated sludge is unclear due to the complexity of EPS.In this work,threedimensional excitation emission matrix fluorescence spectroscopy（3DEEM）,fluorescence quenching titration and Fourier transform infrared spectroscopy（FT-IR） were used to explore the binding behavior and mechanism between Al 3＋ and EPS.The results showed that two fluorescence peaks of tyrosineand tryptophan-like substances were identified in the loosely bound-extracellular polymeric substances（LB-EPS）,and three peaks of tyrosine-,tryptophanand humic-like substances were identified in the tightly boundextracellular polymeric substances（TB-EPS）.It was found that these fluorescence peaks could be quenched with Al 3＋ at the dosage of 3.0 mg/L,which demonstrated that strong interactions took place between the EPS and Al 3＋.The conditional stability constants for Al 3＋ and EPS were determined by the Stern-Volmer equation.As to the binding mechanism,the-OH,N-H,C=O,C-N groups and the sulfurand phosphorus-containing groups showed complexation action,although the groups in the LB-EPS and TB-EPS showed different behavior.The TB-EPS have stronger binding ability to Al 3＋ than the LB-EPS,and TB-EPS play an important role in the interaction with Al 3＋.展开更多
The fluorogenic property of guaiacol was exploited for the first time to analyze the interaction with target protein as a probe by molecular modeling, fluorescence, circular dichroism (CD) and Fourier transform infrar...The fluorogenic property of guaiacol was exploited for the first time to analyze the interaction with target protein as a probe by molecular modeling, fluorescence, circular dichroism (CD) and Fourier transform infrared (FT-IR) spectroscopy. Molecular docking was performed to reveal the possible binding mode or mechanism and suggested that guaiacol can strongly bind to human immu- noglobulin (HIgG). It is considered that guaiacol binds to HIgG mainly by a hydrophobic interaction and there are two hydrogen bond interactions between the drug and the residues LEU 80 and ASP 65, which is in good agreement with the results from the experimental thermodynamic parameters (the enthalpy change △H0 and the entropy change △S0 were calculated to be 65.55 kJ·mol-1 and 132.95 J·mol-1·K-1 according to the Vant’ Hoff equation). Data obtained by the fluorescence spectroscopy indicated that binding of guaiacol with HIgG leads to dramatic enhancement in the fluorescence emission intensity along with significant occurrence of efficient Frster resonance energy transfer (FRET) from the residue of HIgG to the protein bound guaiacol. From the low value of fluorescence anisotropy (r = 0.06), it is argued that the probe molecule is located in the motionally unrestricted environment of the protein. The alterations of protein’s secondary structure in the presence of guaiacol in aqueous solution were quantitatively calculated by the evidences from FT-IR and CD spectroscopes.展开更多
Compared with the conventional first near-infrared(NIR-I,700900 nm)window,the short-wave infrared region(SWIR,900—1700nm)possesses the merits of the increasing tissue penetration depths and the suppression of scatter...Compared with the conventional first near-infrared(NIR-I,700900 nm)window,the short-wave infrared region(SWIR,900—1700nm)possesses the merits of the increasing tissue penetration depths and the suppression of scattering background,leading to great potential for in vivo imaging.Based on the limitations of the common spectral domain,and the superiority of the time-dimension,time-resolved imaging eliminates the auto-fuorescence in the biological tissue,thus supporting higher signal-to-noise ratio and sensitivities.The imaging technique is not affected by the difference in tissue composition or thickness and has the practical value of quan-titative in vivo detection.Almost all the relevant time-resolved imaging was carried out around lanthanide-doped upconversion nanomaterials,owing to the advantages of ultralong luminescence lifetime,excellent photostability,controllable morphology,easy surface modification and various strategies of regulating lifetime.Therefore,this review presents the research progress of SWIR time-resolved imaging technology based on nanomaterials doped with lanthanide ions as luminescence centers in recent years.展开更多
BACKGROUND Gastric cancer is a common malignant tumor of the digestive system worldwide,and its early diagnosis is crucial to improve the survival rate of patients.Indocyanine green fluorescence imaging(ICG-FI),as a n...BACKGROUND Gastric cancer is a common malignant tumor of the digestive system worldwide,and its early diagnosis is crucial to improve the survival rate of patients.Indocyanine green fluorescence imaging(ICG-FI),as a new imaging technology,has shown potential application prospects in oncology surgery.The meta-analysis to study the application value of ICG-FI in the diagnosis of gastric cancer sentinel lymph node biopsy is helpful to comprehensively evaluate the clinical effect of this technology and provide more reliable guidance for clinical practice.AIM To assess the diagnostic efficacy of optical imaging in conjunction with indocya-nine green(ICG)-guided sentinel lymph node(SLN)biopsy for gastric cancer.METHODS Electronic databases such as PubMed,Embase,Medline,Web of Science,and the Cochrane Library were searched for prospective diagnostic tests of optical imaging combined with ICG-guided SLN biopsy.Stata 12.0 software was used for analysis by combining the"bivariable mixed effect model"with the"midas"command.The true positive value,false positive value,false negative value,true negative value,and other information from the included literature were extracted.A literature quality assessment map was drawn to describe the overall quality of the included literature.A forest plot was used for heterogeneity analysis,and P<0.01 was considered to indicate statistical significance.A funnel plot was used to assess publication bias,and P<0.1 was considered to indicate statistical significance.The summary receiver operating characteristic(SROC)curve was used to calculate the area under the curve(AUC)to determine the diagnostic accuracy.If there was interstudy heterogeneity(I2>50%),meta-regression analysis and subgroup analysis were performed.analysis were performed.RESULTS Optical imaging involves two methods:Near-infrared(NIR)imaging and fluorescence imaging.A combination of optical imaging and ICG-guided SLN biopsy was useful for diagnosis.The positive likelihood ratio was 30.39(95%CI:0.92-1.00),the sensitivity was 0.95展开更多
基金Project supported by the National Key R&D Program of China(2017YFA0207303)National Science Fund for Distinguished Young Scholars(21725502)+1 种基金Key Basic Research Program of Science and Technology Commission of Shanghai Municipality(17JC1400100)Intergovernmental International Cooperation Project of Science and Technology Commission of Shanghai Municipality(19490713100)。
文摘The second near-infrared(NIR-Ⅱ,1000-1700 nm)window provides a superior optical platform with high resolution,deep penetration and high signal-to-noise ratios(SNRs),which results from the intrinsic low scattering and auto fluorescence in biological tissues.As one of the promising NIR-Ⅱemitting probes,lanthanide based nanoparticles(LnNPs)exhibit high photo stability and chemostability,long photoluminescence lifetimes,low long-term cytotoxicity and narrow emission bandwidths.All these merits have spurred the evolution of related bio-optics and a variety of biomedical applications of LnNPs.This mini-review discusses the most recent advances in both the design-the composition and surface modifications-and the applications of NIR-Ⅱemitting LnNPs in bioimaging,disease diagnosis and therapy.We also summarize the current limits and challenges facing the applications of LnNPs as well as discuss the directions of future development.
文摘AIM: To investigate feasibility and accuracy of near-infrared fluorescence imaging using indocyanine green: nanocolloid for sentinel lymph node (SLN) detection in gastric cancer.METHODS: A prospective, single-institution, phase I feasibility trial was conducted. Patients suffering from gastric cancer and planned for gastrectomy were included. During surgery, a subserosal injection of 1.6 mL ICG:Nanocoll was administered around the tumor. NIR fluorescence imaging of the abdominal cavity was performed using the Mini-FLARE™ NIR fluorescence imaging system. Lymphatic pathways and SLNs were visualized. Of every detected SLN, the corresponding lymph node station, signal-to-background ratio and histopathological diagnosis was determined. Patients underwent standard-of-care gastrectomy. Detected SLNs outside the standard dissection planes were also resected and evaluated.RESULTS: Twenty-six patients were enrolled. Four patients were excluded because distant metastases were found during surgery or due to technical failure of the injection. In 21 of the remaining 22 patients, at least 1 SLN was detected by NIR Fluorescence imaging (mean 3.1 SLNs; range 1-6). In 8 of the 21 patients, tumor-positive LNs were found. Overall accuracy of the technique was 90% (70%-99%; 95%CI), which decreased by higher pT-stage (100%, 100%, 100%, 90%, 0% for respectively Tx, T1, T2, T3, T4 tumors). All NIR-negative SLNs were completely effaced by tumor. Mean fluorescence signal-to-background ratio of SLNs was 4.4 (range 1.4-19.8). In 8 of the 21 patients, SLNs outside the standard resection plane were identified, that contained malignant cells in 2 patients.CONCLUSION: This study shows successful use of ICG:Nanocoll as lymphatic tracer for SLN detection in gastric cancer. Moreover, tumor-containing LNs outside the standard dissection planes were identified.
基金funded by the National Natural Science Foundation of China (No. 51138009,50921064)
文摘Aluminum flocculant can enhance the flocculating performance of activated sludge.However,the binding mechanism of aluminum ion（Al 3＋） and extracellular polymeric substances（EPS） in activated sludge is unclear due to the complexity of EPS.In this work,threedimensional excitation emission matrix fluorescence spectroscopy（3DEEM）,fluorescence quenching titration and Fourier transform infrared spectroscopy（FT-IR） were used to explore the binding behavior and mechanism between Al 3＋ and EPS.The results showed that two fluorescence peaks of tyrosineand tryptophan-like substances were identified in the loosely bound-extracellular polymeric substances（LB-EPS）,and three peaks of tyrosine-,tryptophanand humic-like substances were identified in the tightly boundextracellular polymeric substances（TB-EPS）.It was found that these fluorescence peaks could be quenched with Al 3＋ at the dosage of 3.0 mg/L,which demonstrated that strong interactions took place between the EPS and Al 3＋.The conditional stability constants for Al 3＋ and EPS were determined by the Stern-Volmer equation.As to the binding mechanism,the-OH,N-H,C=O,C-N groups and the sulfurand phosphorus-containing groups showed complexation action,although the groups in the LB-EPS and TB-EPS showed different behavior.The TB-EPS have stronger binding ability to Al 3＋ than the LB-EPS,and TB-EPS play an important role in the interaction with Al 3＋.
文摘The fluorogenic property of guaiacol was exploited for the first time to analyze the interaction with target protein as a probe by molecular modeling, fluorescence, circular dichroism (CD) and Fourier transform infrared (FT-IR) spectroscopy. Molecular docking was performed to reveal the possible binding mode or mechanism and suggested that guaiacol can strongly bind to human immu- noglobulin (HIgG). It is considered that guaiacol binds to HIgG mainly by a hydrophobic interaction and there are two hydrogen bond interactions between the drug and the residues LEU 80 and ASP 65, which is in good agreement with the results from the experimental thermodynamic parameters (the enthalpy change △H0 and the entropy change △S0 were calculated to be 65.55 kJ·mol-1 and 132.95 J·mol-1·K-1 according to the Vant’ Hoff equation). Data obtained by the fluorescence spectroscopy indicated that binding of guaiacol with HIgG leads to dramatic enhancement in the fluorescence emission intensity along with significant occurrence of efficient Frster resonance energy transfer (FRET) from the residue of HIgG to the protein bound guaiacol. From the low value of fluorescence anisotropy (r = 0.06), it is argued that the probe molecule is located in the motionally unrestricted environment of the protein. The alterations of protein’s secondary structure in the presence of guaiacol in aqueous solution were quantitatively calculated by the evidences from FT-IR and CD spectroscopes.
基金the National Natural Science Foundation of China(No.81971704)the National Key ResearchandDevelopment Program of China(No.2017YFA0205304)the Translational Medicine Research Fund of National Facility for Translational Medicine(Shanghai)(No.TMSK-2021-117)。
文摘Compared with the conventional first near-infrared(NIR-I,700900 nm)window,the short-wave infrared region(SWIR,900—1700nm)possesses the merits of the increasing tissue penetration depths and the suppression of scattering background,leading to great potential for in vivo imaging.Based on the limitations of the common spectral domain,and the superiority of the time-dimension,time-resolved imaging eliminates the auto-fuorescence in the biological tissue,thus supporting higher signal-to-noise ratio and sensitivities.The imaging technique is not affected by the difference in tissue composition or thickness and has the practical value of quan-titative in vivo detection.Almost all the relevant time-resolved imaging was carried out around lanthanide-doped upconversion nanomaterials,owing to the advantages of ultralong luminescence lifetime,excellent photostability,controllable morphology,easy surface modification and various strategies of regulating lifetime.Therefore,this review presents the research progress of SWIR time-resolved imaging technology based on nanomaterials doped with lanthanide ions as luminescence centers in recent years.
文摘BACKGROUND Gastric cancer is a common malignant tumor of the digestive system worldwide,and its early diagnosis is crucial to improve the survival rate of patients.Indocyanine green fluorescence imaging(ICG-FI),as a new imaging technology,has shown potential application prospects in oncology surgery.The meta-analysis to study the application value of ICG-FI in the diagnosis of gastric cancer sentinel lymph node biopsy is helpful to comprehensively evaluate the clinical effect of this technology and provide more reliable guidance for clinical practice.AIM To assess the diagnostic efficacy of optical imaging in conjunction with indocya-nine green(ICG)-guided sentinel lymph node(SLN)biopsy for gastric cancer.METHODS Electronic databases such as PubMed,Embase,Medline,Web of Science,and the Cochrane Library were searched for prospective diagnostic tests of optical imaging combined with ICG-guided SLN biopsy.Stata 12.0 software was used for analysis by combining the"bivariable mixed effect model"with the"midas"command.The true positive value,false positive value,false negative value,true negative value,and other information from the included literature were extracted.A literature quality assessment map was drawn to describe the overall quality of the included literature.A forest plot was used for heterogeneity analysis,and P<0.01 was considered to indicate statistical significance.A funnel plot was used to assess publication bias,and P<0.1 was considered to indicate statistical significance.The summary receiver operating characteristic(SROC)curve was used to calculate the area under the curve(AUC)to determine the diagnostic accuracy.If there was interstudy heterogeneity(I2>50%),meta-regression analysis and subgroup analysis were performed.analysis were performed.RESULTS Optical imaging involves two methods:Near-infrared(NIR)imaging and fluorescence imaging.A combination of optical imaging and ICG-guided SLN biopsy was useful for diagnosis.The positive likelihood ratio was 30.39(95%CI:0.92-1.00),the sensitivity was 0.95
