Interaction between indigenous fungal strains in preventing ochratoxin A(OTA) production by Penicillium verrucosum and Penicillium nordicum was studied in 100 mL of Czapek yeast autolysate(CYA) medium in a 250-mL &quo...Interaction between indigenous fungal strains in preventing ochratoxin A(OTA) production by Penicillium verrucosum and Penicillium nordicum was studied in 100 mL of Czapek yeast autolysate(CYA) medium in a 250-mL "U" shaped culture vessel in one end for 3 days. At the end of incubation period, test fungi inoculated and incubated at 27 ± 2℃ for another 14 days to study the inhibition of OTA production was estimated by high performance liquid chromatography(HPLC). Total inhibition of OTA production was recorded with Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, Aspergillus ustus, Fusarium culmorum, Fusarium graminarium, Fusarium proliferatum, Penicillium chrysogenum, Penicillium expansum and Trichoderma viridae. A significant correlation coefficient(r) on growth(0.493, P < 0.0003) and OTA production(0.785, P < 0.0001) was observed between the tested Penicillium species and other coexisting fungi. In conclusion, the present investigation revealed that those indigenous fungi are necessary to minimize potential losses to the poultry farmer and toxicological hazards to the consumer as biological control agent in different foods and feeds.展开更多
【目的】为探讨不同盐度土壤土著丛枝菌根(Arbuscular mycorrhizae,AM)真菌对苗期棉花根系形态的影响。【方法】采用in-growth生长系统进行了田间原位研究。【结果】低盐(σ1.0 m S·cm^(-1))、中盐(σ2.1 m S·cm^(-1))、高盐(...【目的】为探讨不同盐度土壤土著丛枝菌根(Arbuscular mycorrhizae,AM)真菌对苗期棉花根系形态的影响。【方法】采用in-growth生长系统进行了田间原位研究。【结果】低盐(σ1.0 m S·cm^(-1))、中盐(σ2.1 m S·cm^(-1))、高盐(σ4.0 m S·cm^(-1))土壤条件下,土著AM真菌侵染显著增加了棉花株高、叶片数、蕾数、叶面积指数,说明土著AM真菌能够改善棉花的生长提高耐盐能力。土著AM真菌显著提高了总根长、根体积、根表面积以及直径(d)≤0.5 mm细根根长,低盐土壤条件下土著AM真菌显著增加了d≤0.5 mm细根的根长占总根长的百分比。相关分析表明,棉花总根长、d≤0.5 mm的细根根长与菌根侵染率、叶片磷、钾浓度及吸收量呈显著正相关,与叶片钠浓度呈显著负相关关系;棉花总生物量与叶片吸磷量、吸钾量极显著正相关。【结论】上述结果说明盐渍化土壤中的土著AM真菌侵染对棉花植株的磷、钾等营养状况的改善和棉株钠、氯效应的降低,与土著AM真菌促进细根生长、提高细根根长占总根长的比例有关。展开更多
基金supported by University Grants Commission (F.No. 36-129/2008), New Delhi, India and the Head Department of Microbiology, Kakatiya University
文摘Interaction between indigenous fungal strains in preventing ochratoxin A(OTA) production by Penicillium verrucosum and Penicillium nordicum was studied in 100 mL of Czapek yeast autolysate(CYA) medium in a 250-mL "U" shaped culture vessel in one end for 3 days. At the end of incubation period, test fungi inoculated and incubated at 27 ± 2℃ for another 14 days to study the inhibition of OTA production was estimated by high performance liquid chromatography(HPLC). Total inhibition of OTA production was recorded with Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, Aspergillus ustus, Fusarium culmorum, Fusarium graminarium, Fusarium proliferatum, Penicillium chrysogenum, Penicillium expansum and Trichoderma viridae. A significant correlation coefficient(r) on growth(0.493, P < 0.0003) and OTA production(0.785, P < 0.0001) was observed between the tested Penicillium species and other coexisting fungi. In conclusion, the present investigation revealed that those indigenous fungi are necessary to minimize potential losses to the poultry farmer and toxicological hazards to the consumer as biological control agent in different foods and feeds.
文摘【目的】为探讨不同盐度土壤土著丛枝菌根(Arbuscular mycorrhizae,AM)真菌对苗期棉花根系形态的影响。【方法】采用in-growth生长系统进行了田间原位研究。【结果】低盐(σ1.0 m S·cm^(-1))、中盐(σ2.1 m S·cm^(-1))、高盐(σ4.0 m S·cm^(-1))土壤条件下,土著AM真菌侵染显著增加了棉花株高、叶片数、蕾数、叶面积指数,说明土著AM真菌能够改善棉花的生长提高耐盐能力。土著AM真菌显著提高了总根长、根体积、根表面积以及直径(d)≤0.5 mm细根根长,低盐土壤条件下土著AM真菌显著增加了d≤0.5 mm细根的根长占总根长的百分比。相关分析表明,棉花总根长、d≤0.5 mm的细根根长与菌根侵染率、叶片磷、钾浓度及吸收量呈显著正相关,与叶片钠浓度呈显著负相关关系;棉花总生物量与叶片吸磷量、吸钾量极显著正相关。【结论】上述结果说明盐渍化土壤中的土著AM真菌侵染对棉花植株的磷、钾等营养状况的改善和棉株钠、氯效应的降低,与土著AM真菌促进细根生长、提高细根根长占总根长的比例有关。