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Effect of maternal age on the outcomes of in vitro fertilization and embryo transfer(IVF-ET) 被引量:24
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作者 YAN JunHao WU KeLiang +2 位作者 TANG Rong DING LingLing CHEN Zi-Jiang 《Science China(Life Sciences)》 SCIE CAS 2012年第8期694-698,共5页
This is a retrospective,observational study to evaluate the effect of maternal age on the outcomes of in vitro fertilization and embryo transfer(IVF-ET).11830 IVF-ET cycles from 10268 women were included.Four groups o... This is a retrospective,observational study to evaluate the effect of maternal age on the outcomes of in vitro fertilization and embryo transfer(IVF-ET).11830 IVF-ET cycles from 10268 women were included.Four groups of different maternal age periods were compared.The groups were 21 30 years old group(4549 cycles),31-35 years old group(4424 cycles),36-40 years old group(2429 cycles),and over 40 years old group(428 cycles).The mean starting dose of Gn and mean total dose of Gn in each cycle were significantly higher(P<0.01),while the mean retrieved oocyte number was significantly lower(P<0.01) in groups of higher maternal age period than those in each of the lower groups.The biochemical pregnancy rate and the clinical pregnancy rate were significantly lower(P<0.01),while the miscarriage rate was significantly higher(P<0.01) in groups of higher maternal age period than those in the lower groups.No difference was found in two-pronuclear zygotes(2PN) rate and good quality embryo rate among different groups.Birth defect rate was also comparable in the born babies in different groups.In the group with patients' age over 40 years old,the pregnancy rate was 26.87%,the clinical pregnancy rate was 19.39%,while the miscarriage rate after clinical pregnancy was 36.14%.To draw the conclusion,patients with higher maternal age had worse IVF outcomes.In women of fertile age,patients between 20 and 30 years old have the best IVF outcomes.Patients over 40 years old have poor IVF outcome and high miscarriage rate,which suggested the necessity of preimplantation genetic screening(PGS). 展开更多
关键词 in vitro fertilization OUTCOME pregnancy rate miscarriage rate birth defect maternal age
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Sperm chromatin structure assay results after swim-up are related only to embryo quality but not to fertilization and pregnancy rates following IVF 被引量:16
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作者 Zhi-Hong Niu Hui-Juan Shi +3 位作者 Hui-Qin Zhang Ai-Jun Zhang Yi-Juan Sun Yun Feng 《Asian Journal of Andrology》 SCIE CAS CSCD 2011年第6期862-866,共5页
The aim of this study was to investigate whether the sperm chromatin structure assay (SCSA) results after swim-up are related to fertilization rates, embryo quality and pregnancy rates following in vitrofertilizati... The aim of this study was to investigate whether the sperm chromatin structure assay (SCSA) results after swim-up are related to fertilization rates, embryo quality and pregnancy rates following in vitrofertilization (IVF). A total of 223 couples undergoing IVF in our hospital from October 2008 to September 2009 were included in this study. Data on the IVF process and sperm chromatin structure assay results were collected. Fertilization rate, embryo quality and IVF success rates of different DNA fragmentation index (DFI) subgroups and high DNA stainability (HDS) subgroups were compared. There were no significant differences in fertilization rate, clinical pregnancy or delivery rates between the DFI and HDS subgroups. However, the group with abnormal DFI had a lower good embryo rate. So, we concluded that the SCSA variables, either DFI or HDS after swim-up preparation, were not valuable in predicting fertilization failure or pregnancy rate, but an abnormal DFI meant a lower good embryo rate following IVF. 展开更多
关键词 EMBRYO fertilization in-vitro fertilization sperm chromatin structure assay
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A highly homozygous and parthenogenetic human embryonic stem cell line derived from a one-pronuclear oocyte following in vitro fertilization procedure 被引量:15
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作者 Ge Lin 《Cell Research》 SCIE CAS CSCD 2007年第12期999-1007,共9页
Homozygous human embryonic stem cells (hESCs) are thought to be better cell sources for hESC banking because their human leukocyte antigen (HLA) haplotype would strongly increase the degree of matching for certain... Homozygous human embryonic stem cells (hESCs) are thought to be better cell sources for hESC banking because their human leukocyte antigen (HLA) haplotype would strongly increase the degree of matching for certain populations with relatively smaller cohorts of cell lines. Homozygous hESCs can be generated from parthenogenetic embryos, but only heterozygous hESCs have been established using the current strategy to artificially activate the oocyte without second polar body extrusion. Here we report the first successful derivation of a human homozygous ESC line (chHES- 32) from a one-pronuclear oocyte following routine in vitro fertilization treatment, chHES-32 cells express common markers and genes with normal hESCs. They have been propagated in an undifferentiated state for more than a year (〉P50) and have maintained a stable karyotype of 46, XX. When differentiated in vivo and in vitro, chHES-32 cells can form derivatives from all three embryonic germ layers. The almost undetectable expression of five paternally expressed imprinted genes and their HLA genotype identical to the oocyte donor indicated their parthenogenetic origin. Using genome-wide single-nucleotide polymorphism analysis and DNA fingerprinting, the homozygosity of chHES-32 cells was further confirmed. The results indicated that ‘ unwanted' one-pronuclear oocytes might be a potential source for human homozygous and parthenogenetic ESCs, and suggested an alternative strategyfor obtaining homozygous hESC lines from parthenogenetic haploid oocytes. 展开更多
关键词 human embryonic stem cells HOMOZYGOSITY PARTHENOGENESIS pronuclear in vitro fertilization
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阶段式健康教育模式在试管婴儿中的护理效果及对知晓率的影响研究 被引量:14
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作者 吴碧玉 周丽端 《中国医药科学》 2020年第18期131-133,137,共4页
目的研究阶段式健康教育模式在试管婴儿中的护理效果及对知晓率的影响。方法将2018年9月~2020年1月于本院行试管婴儿的70例患者作为研究样本,采用随机数字表法将其分为参照组与研究组,每组各35例。参照组采用常规护理,研究组患者采用常... 目的研究阶段式健康教育模式在试管婴儿中的护理效果及对知晓率的影响。方法将2018年9月~2020年1月于本院行试管婴儿的70例患者作为研究样本,采用随机数字表法将其分为参照组与研究组,每组各35例。参照组采用常规护理,研究组患者采用常规护理+阶段式健康教育。观察和比较两组自护能力评分、知晓率。结果研究组患者干预后的自护能力评分高于参照组,差异有统计学意义(P<0.05)。研究组知晓率高于参照组,差异有统计学意义(P<0.05)。结论在试管婴儿中采用阶段式健康教育模式可有效提高患者健康知识知晓率,还能提升患者的自护能力,确保试管婴儿效果。 展开更多
关键词 阶段式健康教育模式 试管婴儿 护理效果 知晓率
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针灸治疗在体外受精—胚胎移植技术中的临床应用研究 被引量:14
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作者 严红莲 何淑祯 +3 位作者 邢彦君 罗晓丹 林冰 赖慧华 《广州医药》 2015年第1期13-16,共4页
目的探讨针灸治疗在体外受精—胚胎移植技术的临床效果。方法以2013年8月—2015年8月在顺德妇幼保健院生殖科,因不孕进入IVF/ICSI周期的108例患者为研究对象,按照随机自愿原则将其分为三组,针刺组、安慰针刺组、对照组各36例,针刺组:在... 目的探讨针灸治疗在体外受精—胚胎移植技术的临床效果。方法以2013年8月—2015年8月在顺德妇幼保健院生殖科,因不孕进入IVF/ICSI周期的108例患者为研究对象,按照随机自愿原则将其分为三组,针刺组、安慰针刺组、对照组各36例,针刺组:在移植前24小时、移植后30分钟进行相关的针刺治疗,安慰针刺组:针刺穴位与妊娠无关,对照组:不使用针刺疗法,比较三组临床效果。结果治疗后针刺组Ⅰ型内膜血流类型及PI、RI、S/D低于治疗前,Ⅱ+Ⅲ型内膜下血流类型高于治疗前,差异有统计学意义(P<0.05)。针刺组总有效率高于安慰针刺组、对照组,三组差异有统计学意义(P<0.05)。针刺组种植成功率、妊娠成功率及活产率均高于安慰针刺组、对照组,三组差异有统计学意义(P<0.05)。结论在体外受精—胚胎移植技术中为患者实施针灸治疗可改善子宫内膜下血流情况,提高临床疗效、改善妊娠结局。 展开更多
关键词 体外受精 胚胎移植 针灸
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Association between serum estradiol level on the human chorionic gonadotrophin administration day and clinical outcome 被引量:12
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作者 Xin Li Cheng Zeng +3 位作者 Jing Shang Sheng Wang Xue-Lian Gao Qing Xue 《Chinese Medical Journal》 SCIE CAS CSCD 2019年第10期1194-1201,共8页
Background: Estradiol, as an important hormone in follicular development and endometrial receptivity, is closely related to clinical outcomes of fresh in vitro fertilization embryo transfer (IVF-ET) cycles. The aim of... Background: Estradiol, as an important hormone in follicular development and endometrial receptivity, is closely related to clinical outcomes of fresh in vitro fertilization embryo transfer (IVF-ET) cycles. The aim of this retrospective study was to evaluate the association between elevated serum estradiol (E2) levels on the day of human chorionic gonadotrophin (hCG) administration and IVF-ET pregnancy and birth outcomes. Methods: A total of 1771 infertile patients with their first fresh IVF-ET cycles were analyzed retrospectively between January 2011 and January 2016 in Peking University First Hospital. Patients were categorized by serum E2 levels on the day of hCG administration into six groups: group 1 (serum E2 levels < 1000 pg/mL, n = 205), group 2 (serum E2 levels 1001-2000 pg/mL, n = 457), group 3 (serum E2 levels 2001-3000 pg/mL, n = 425), group 4 (serum E2 levels 3001-4000 pg/mL, n = 310), group 5 (serum E2 levels 4001- 5000 pg/mL, n = 237), and group 6 (serum E2 levels > 5000 pg/mL, n — 137). The retrieved oocyte and Mil oocyte numbers and implantation and clinical pregnancy rates of the groups were compared as the first objective of the study. For the 360 women with singleton births among all patients, the area under the corresponding receiver operating characteristic curve (ROC curve) was calculated to assess the predictive value of the E2 change for the probability of low birth weight (LBW) infants as the second objective. Results: The retrieved oocyte and Mil oocyte numbers and implantation and clinical pregnancy rates gradually increased from groups 1 to 5 but decreased in group 6. The parameters of group 1 were statistically worse than those of the other groups, from group 2 to group 6 (the number of retrieved oocytes, t = 13.096, t = 23.307, t = 23.086, t = 26.376, t = 19.636, P < 0.003;the number of retrieved Mil oocytes, t = 10.856, t = 20.868, t = 21.874, t = 23.374, t = 19.092, P < 0.003;the implantation rate,χ^2= 12.179,χ^2= 22.239,χ^2= 23.993,χ^2= 23.344,χ^2= 16.758, P < 0.003;the cl 展开更多
关键词 ESTRADIOL in vitro fertilization Clinical PREGNANCY rate Low BIRTH weight
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生精散对人精子运动能力和受精能力的影响 被引量:11
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作者 杨伟娜 孙伟 +2 位作者 管群 张琪瑶 孔风云 《辽宁中医药大学学报》 CAS 2008年第10期151-153,共3页
目的:研究含生精散血清对人精子运动能力和受精能力的影响。方法:通过人精子与生精散血清共培养,观察生精散对人精子运动能力(精子运动CASA分析)、精子与卵透明带结合能力的影响。结果:含药血清加入共培养可明显提高人精子运动速度[精... 目的:研究含生精散血清对人精子运动能力和受精能力的影响。方法:通过人精子与生精散血清共培养,观察生精散对人精子运动能力(精子运动CASA分析)、精子与卵透明带结合能力的影响。结果:含药血清加入共培养可明显提高人精子运动速度[精子运动平均路径速度(VAP)、精子曲线速度(VCL)、精子直线运动速度(VSL)](P<0.05)精子头部侧摆幅度(ALH)和鞭毛摆动频率(BCF)(P<0.05),可增强精子与卵透明带结合能力(P<0.05)。结论:生精散与精子共培养具有提高人精子运动能力和精卵结合能力的作用。 展开更多
关键词 生精散 含药血清 精子运动 顶体反应 体外受精
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Factors associated with the incidence of ectopic pregnancy in women undergoing assisted reproductive treatment 被引量:11
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作者 Xiao-Ying Jin Chao Li +6 位作者 Wen Xu Liu Liu Min-Ling Wei Hai-Yi Fei Jing Li Feng Zhou Song-Ying Zhang 《Chinese Medical Journal》 SCIE CAS CSCD 2020年第17期2054-2060,共7页
Background:Ectopic pregnancy(EP)is a common complication in women undergoing assisted reproductive treatment,but the underlying causes for this remain unclear.This study aimed to explore factors affecting the incidenc... Background:Ectopic pregnancy(EP)is a common complication in women undergoing assisted reproductive treatment,but the underlying causes for this remain unclear.This study aimed to explore factors affecting the incidence of EP in in vitro fertilization(IVF)/intracytoplasmic sperm injection(ICSI).Methods:This was a retrospective study on the incidence of EP in IVF/ICSI cycles between January 1,2013 and December 31,2017.Patient age,infertility diagnosis(tubal factor or not),primary or secondary infertility,type of cycle(frozen-thawed or fresh),type of embryo(s)transferred(cleavage embryo or blastocyst),number of embryos transferred(one,two,or three),previous history of EP,and endometrial combined thickness were analyzed to explore their relationships with the incidence of EP.Based on clinical typing results,the patients were divided into an EP group or a non-EP group.Categorical variables were analyzed using Chi-squared test or Fisher exact test.Logistic regression analysis was performed to explore their associations with the incidence of EP.Results:The percentage of patients with primary infertility in EP group was significantly lower than that in non-EP group(31.3%vs.46.7%,χ^2=26.032,P<0.001).The percentage of patients with tubal infertility in EP group was also significantly higher than that in non-EP group(89.2%vs.63.6%,χ^2=77.410,P<0.001).The percentages of patients with transfer of cleavage-stage embryo or blastocyst(91.4%vs.84.4%,χ^2=10.132,P=0.001)and different endometrial combined thickness(ECT)(χ^2=18.373,P<0.001)differed significantly between EP and non-EP groups.For patients who had a previous history of one to four EPs,the percentage of patients undergoing transfer of a cleavage-stage embryo was significantly higher in EP group than that in non-EP group(92.2%vs.77.6%,χ^2=13.737,P<0.001).In multivariate logistic regression analysis,tubal infertility was strongly associated with EP(adjusted odds ratio:3.995,95%confidence interval:2.706-5.897,P<0.001).Conclusions:In IVF/ICSI cycles,transfer of a blasto 展开更多
关键词 Prognostic factor Ectopic pregnancy Tubal factor In vitro fertilization Intracytoplasmic sperm injection
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囊胚培养的相关因素分析 被引量:11
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作者 黄志辉 余敏 +1 位作者 伍琼芳 辛才林 《江西医药》 CAS 2013年第4期305-308,共4页
目的探讨体外受精(IVF)和精子卵胞浆内注射技术(ICSI)治疗周期中体外培养囊胚形成的影响因素。方法将卵裂期第3d(D3)冷冻后剩余胚胎进行体外延长培养至囊胚期,观察囊胚形成情况。结果共收集7438个冷冻后剩余胚胎,经序贯培养,形成1382个... 目的探讨体外受精(IVF)和精子卵胞浆内注射技术(ICSI)治疗周期中体外培养囊胚形成的影响因素。方法将卵裂期第3d(D3)冷冻后剩余胚胎进行体外延长培养至囊胚期,观察囊胚形成情况。结果共收集7438个冷冻后剩余胚胎,经序贯培养,形成1382个优质囊胚(18.58%)。D3胚胎中6个细胞的优质囊胚形成率(10.34%)显著高于4-5个细胞的优质囊胚形成率(5.45%),差异有统计学意义(P=0.011);7-9个细胞的优质囊胚形成率(19.95%)显著高于6个细胞的优质囊胚形成率(10.34%),≥10个细胞的优质囊胚形成率(27.47%)显著高于7-9个细胞的优质囊胚形成率(19.95%),差异均有统计学意义(P<0.001)。D2胚胎中4个细胞的优质囊胚形成率(25.40%)显著高于3个细胞、5个细胞的优质囊胚形成率(10.26%,15.41%),差异有统计学意义(P<0.001)。碎片评级I-II级D2、D3胚胎的囊胚形成率均显著高于III-IV级的胚胎,差异有统计学意义(P<0.001)。结论体外延长培养能有效筛选具有发育潜力的胚胎,优质囊胚形成与D2、D3胚胎的卵裂球数及碎片评级密切相关。 展开更多
关键词 体外受精 囊胚培养 卵裂球 胚胎碎片
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Dynamic measurements of serum inhibin B and estradiol: a predictive evaluation of ovarian response to gonadotrophin stimulation in the early stage of IVF treatment 被引量:9
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作者 Ming-fang MIAO He-feng HUANG 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2009年第1期35-45,共11页
Objective: We dynamically measured serum inhibin B and estradiol in the early stage of hormonal stimulation to predict the ovarian response in in vitro fertilization (IVF) treatment. Methods: A total of 57 patien... Objective: We dynamically measured serum inhibin B and estradiol in the early stage of hormonal stimulation to predict the ovarian response in in vitro fertilization (IVF) treatment. Methods: A total of 57 patients (〈40 years of age) who underwent the first cycle of long protocol IVF or introcytoplasmic sperm injection (ICSI) treatment were included. Serum inhibin B, estradiol, follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels were measured four times: (1) on Day 3 of the menstrual cycle (basal); (2) on the day before the first administration ofgonadotrophin (Gn) (Day 0); (3) on Day 1 of Gn therapy; and (4) on Day 5 of Gn therapy. Comparisons of these measurements with ovarian responses and pregnancy outcomes were made and analyzed statistically. Results: (1) On Day 1 and Day 5 of recombinant FSH (rFSH) stimulation, ovarian response, i.e., numbers of follicles, oocytes, fertilized oocytes, and embryos, had a positive correlation (rs=0.46-0.61, P 0.000) with raised inhibin B and estradiol concentrations, but a negative correlation (rs=-0.67--0.38, P=0.000 or P〈0.01) with total rFSH dose and total days ofrFSH stimulation. (2) No significant variation (P〉0.05) between the pregnant and non-pregnant groups on the basis of mean age or on all hormone concentrations at four times of the IVF cycle was observed. However, all the seven patients aged 〉35 years did not reach pregnancy. Conclusions: (1) Serum inhibin B and estradiol concentrations obtained shortly after Gn therapy may offer an accurate and early prediction of ovarian response; (2) Low levels of serum inhibin B and estradiol obtained shortly after Gn stimulation indicate the need for a longer period of Gn treatment and a higher daily dosage; (3) No obvious pregnancy difference among patients of age 〈35 years was found; however, IVF pregnancy outcome is significantly lower in women of age 〉35 years. 展开更多
关键词 Inhibin B ESTRADIOL Ovarian response In vitro fertilization (IVF) PREGNANCY
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卵巢低反应患者联用克龄蒙和坤泰胶囊对卵巢储备功能和体外受精结局的影响 被引量:9
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作者 彭敏 《临床和实验医学杂志》 2015年第13期1099-1102,共4页
目的研究克龄蒙联合坤泰胶囊预处理对卵巢低反应患者卵巢储备功能及体外受精结局的影响。方法纳入首次体外受精-胚胎移植(IVF-ET)失败,欲行二次IVF-ET,且已证实为卵巢低反应患者100例。随机均分为A、B、C、D四组,A组不行预处理,B组行克... 目的研究克龄蒙联合坤泰胶囊预处理对卵巢低反应患者卵巢储备功能及体外受精结局的影响。方法纳入首次体外受精-胚胎移植(IVF-ET)失败,欲行二次IVF-ET,且已证实为卵巢低反应患者100例。随机均分为A、B、C、D四组,A组不行预处理,B组行克龄蒙预处理,C组行坤泰胶囊预处理,D组行克龄蒙联合坤泰胶囊预处理。持续3个月经周期,于第四个月经周期第2天测定患者血清卵泡刺激素(FSH)、促黄体生成素(LH)、雌激素(E2)水平及卵巢动脉收缩峰值流速(PSV),评价患者卵巢储备能力。随访至体外受精结局,统计促性腺激素(Gn)用量、获卵数、受精率、优质胚胎数。结果 1预处理前后对比,B、C、D组血清FSH、LH均有明显下降,PSV均明显上升;预处理后,B、C组血清FSH、LH均明显低于A组,PSV均明显高于A组,同时D组血清FSH、LH明显低于B组及C组,PSV明显高于B组及C组,差异均有统计学意义(P<0.05)。2C、D两组Gn用量明显少于A组或B组;D组获卵数、优质胚胎数明显多于A、B、C三组,差异均具备统计学意义(P<0.05)。结论联合使用克龄蒙和坤泰胶囊有助于提升卵巢低反应患者卵巢储备功能,进而改善体外受精结局。 展开更多
关键词 体外受精 卵巢低反应 克龄蒙 坤泰胶囊
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Accuracy of a combined score of zygote and embryo morphology for selecting the best embryos for IVF 被引量:9
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作者 Yu-li QIAN Ying-hui YE Chen-ming XU Fan JIN He-feng HUANG 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2008年第8期649-655,共7页
Objective:To evaluate the accuracy of a scoring system combining zygote and embryo morphology in predicting the outcome of in vitro fertilization (IVF) treatment. Methods: In a study group, 117 consecutive IVF or intr... Objective:To evaluate the accuracy of a scoring system combining zygote and embryo morphology in predicting the outcome of in vitro fertilization (IVF) treatment. Methods: In a study group, 117 consecutive IVF or intracytoplasmic sperm injection (ICSI) cycles with embryo transfer were carried out and 312 embryos were scored using a combined scoring system (CSS) of zygote and embryo morphology before transplantation. In a control group, a total of 420 IVF or ICSI cycles were carried out and 1176 embryos were scored using a cumulative embryo score (CES). The effects of the combined scoring system on the embryo implantation rate and pregnancy rate per cycle were analyzed. Results: Using the combined scoring system, the embryo implan-tation rate (27.6%) and the clinical pregnancy rate (48.7%) were significantly higher than those in the control group (20.8% and 38.6%, respectively). Also, the implantation rate of embryos scoring ≥70 (38.5%: 82 sacs/213 embryos) was significantly higher (P<0.001) than that of embryos scoring <70 (4%: 4 sacs/99 embryos). The pregnancy rate of patients with embryos scoring ≥70 using the combined scoring system (66.7%) was significantly higher (P<0.001) than that of patients with embryos scoring ≥20 using the cumulative embryo score (59.0%). Conclusion: The results suggest that selecting embryos with a high score (≥70) using the combined scoring system could increase the implantation rate and pregnancy rate, and that using a scoring system combining assessments of human zygotes and pre-implantation embryos might predict IVF outcomes more accurately than using a cumula-tive embryo score. 展开更多
关键词 Embryo score Combined scoring system (CSS) IMPLANTATION In vitro fertilization (IVF) Pregnancy
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两种活动精子分离法的体外受精结局比较 被引量:7
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作者 黄敏珍 邢福祺 +1 位作者 陈士岭 朱伟杰 《生殖与避孕》 CAS CSCD 北大核心 2002年第5期292-294,共3页
目的:评价Percoll密度梯度法和上游法两种活动精子分离方法在体外受精-胚胎移植(IVF-ET)中的效果。对象和方法:以本中心1999年6月~2001年1月完成的362个IVF-ET治疗周期为研究对象,回顾性地比较了两种活动精子分离方法的受精率、卵裂率... 目的:评价Percoll密度梯度法和上游法两种活动精子分离方法在体外受精-胚胎移植(IVF-ET)中的效果。对象和方法:以本中心1999年6月~2001年1月完成的362个IVF-ET治疗周期为研究对象,回顾性地比较了两种活动精子分离方法的受精率、卵裂率、胚胎发育状况和临床妊娠结局。结果:Percoll法和上游法两组比较,受精率分别为71.8%和71.2%,卵裂率88.4%和89.2%,差异均不显著(P>0.05)。取卵后d2,两组胚胎的细胞数分别为2.7±1.3个和3.0±1.0个,差异不显著(P>0.05);两组<20%碎片的优良胚胎率分别为77.6%和65.9%,差异具有统计学意义(P<0.01)。两组临床妊娠率分别为43.7%和36.6%,妊娠周期的活婴出生率分别为70.3%和60.7%,均具有显著性差异(P<0.01)。结论:在IVF-ET治疗不育与不孕中,Percoll法分离的活动精子临床妊娠结局优于上游法。 展开更多
关键词 体外受精 精子 胚胎 妊娠
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EGF和β-巯基乙醇对羔羊卵母细胞体外受精和体外发育的影响 被引量:9
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作者 郭洪 万鹏程 +7 位作者 石文艳 倪建宏 代蓉 毛青青 朱汇 张宾 刘长彬 石国庆 《安徽农业科学》 CAS 2012年第11期6537-6540,共4页
[目的]为优化幼畜卵母细胞体外培养体系提供理论基础。[方法]研究在成熟液中添加EGF和β-巯基乙醇对绵羔羊卵母细胞受精和卵裂、囊胚情况的影响,并与成年绵羊卵母细胞进行比较。[结果]在成熟液中添加不同浓度的EGF对羔羊卵母细胞卵裂率... [目的]为优化幼畜卵母细胞体外培养体系提供理论基础。[方法]研究在成熟液中添加EGF和β-巯基乙醇对绵羔羊卵母细胞受精和卵裂、囊胚情况的影响,并与成年绵羊卵母细胞进行比较。[结果]在成熟液中添加不同浓度的EGF对羔羊卵母细胞卵裂率和囊胚率无显著影响(P>0.05)。添加不同浓度的β-巯基乙醇可以提高羔羊卵母细胞囊胚率,其中添加100μmol/Lβ-巯基乙醇有显著影响(P<0.05),但对卵裂率影响不显著(P>0.05)。羔羊卵母细胞卵裂率和囊胚率均显著低于成年羊(P<0.05)。添加100μmol/L的β-巯基乙醇能显著提高羔羊卵母细胞正常受精率(P<0.05),且与成年羊卵母细胞受精率差异不显著(P>0.05)。未添加β-巯基乙醇多精入卵率显著高于成年羊(P<0.05),而添加100μmol/L的β-巯基乙醇多精入卵率与成年羊差异不显著(P>0.05)。成熟液中不添加β-巯基乙醇时未受精率(20.0%)高于成年羊组(12.3%)和β-巯基乙醇(13.5%),但差异不显著(P>0.05)。[结论]添加100μmol/L的β-巯基乙醇能显著提高羔羊卵母细胞发育能力,提高正常受精的比例并减少多精子受精的发生,但羔羊卵母细胞发育能力仍显著低于成年羊。 展开更多
关键词 绵羔羊 卵母细胞 体外受精 EGF β-巯基乙醇
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体外受精和卵胞浆单精子注射完全受精失败的临床因素分析 被引量:8
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作者 张文红 龙晓林 +4 位作者 刘寒艳 凌家炜 张伟良 李莉 刘见桥 《中山大学学报(医学科学版)》 CAS CSCD 北大核心 2012年第4期499-503,共5页
【目的】分析体外受精(IVF)和卵胞浆内单精子注射(ICSI)治疗中完全受精失败(TFF)发生的临床原因。【方法】收集本中心2007年1月至2011年5月间辅助生殖治疗数据,分IVF组(5778例)、half-ICSI组(146例)、ICSI组(821例)和PESA-ICSI组(274例... 【目的】分析体外受精(IVF)和卵胞浆内单精子注射(ICSI)治疗中完全受精失败(TFF)发生的临床原因。【方法】收集本中心2007年1月至2011年5月间辅助生殖治疗数据,分IVF组(5778例)、half-ICSI组(146例)、ICSI组(821例)和PESA-ICSI组(274例)进行χ2检验、t-检验和Logistic回归分析等统计分析,并对部分ICSI完全受精失败卵母细胞进行免疫荧光染色和激光共聚焦显微镜观察。【结果】IVF组和ICSI组TFF发生率分别为2.1%和2.3%(P>0.05);half-ICSI组IVF部分TFF发生率为21.9%,显著高于其他各组(P<0.01)。除half-ICSI组,其他各组中获成熟卵数≤3的患者TFF发生率高于获成熟卵数>3患者(P<0.01)。单纯畸精子症对IVF和ICSI治疗周期受精结果的影响无统计学意义(P>0.05),且前次IVF受精失败不影响再次ICSI的受精结局(P>0.05),但少精和弱精是辅助生殖治疗周期受精失败的高危影响因素(P<0.05)。【结论】低获卵数是辅助生殖治疗中完全受精失败的高危因素,获取适当数目成熟卵母细胞是治疗成功的关键;畸精子症对IVF或ICSI的受精结局无显著影响;ICSI治疗是前次IVF治疗完全受精失败患者的首选。 展开更多
关键词 体外受精 卵胞浆内单精子注射 完全受精失败
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Assisted Reproduction of Pretreatment with Laparoscopic “Core-pulling” Salpingectomy for Patients with Hydrosalpinx before in vitro Fertilization Embryo Transfer 被引量:7
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作者 Hong-chu BAO Cui-fang HAO +2 位作者 Mei-mei WANG Li-li ZHUANG Xin-rong WANG 《Journal of Reproduction and Contraception》 CAS 2010年第3期147-154,共8页
Objective To investigate the reproduction-assisting effects of laparoscopic "corepulling" salpingectomy for patients with hydrosalpinx on ovarian reserve,responsiveness to stimuli and outcomes of in vitro fertilizat... Objective To investigate the reproduction-assisting effects of laparoscopic "corepulling" salpingectomy for patients with hydrosalpinx on ovarian reserve,responsiveness to stimuli and outcomes of in vitro fertilization and embryo transfer(IVF-ET).Methods Infertile females receiving treatment in Reproductive Medicine Center of Yantai Yuhuangding Hospital due to fallopian tube disorders(n=214) were enrolled from July 2006 to December 2007 and further divided into three groups."Core-pulling" procedure group(group A) consisted of patients receiving pretreatment with "corepulling" salpingectomy due to hydrosalpinx in our center(n=31),wherein 16 patients received IVF-ET.Conventional procedure group(group B) consisted of patients receiving conventional salpingectomy for hydrosalpinx or ectopic pregnancy in our center or other institutes prior to IVF-ET(n=59).Control group(group C) consisted of patients receiving IVF-ET without the history of previous hydrosalpinx or salpingectomy(n=124).Results At baseline,the antral follicle count of group B was significantly less than that of groups A and C(8.6±2.5 vs 8.3±2.0 vs 9.8±2.4).The mature oocytes retrieved numbered less than the other two groups,in a statistically significant manner compared with group C(13.1±5.7 vs 10.6±5.0 vs 12.0±6.2).Patients of groups A and B received more gonadotropin while the dosage of group B differed significantly from group C(31.0±17.7 vs 37.6±8.3 vs 30.0±4.6).E2 level on hCG injection day was slightly lower in group B than in the other two groups,statistically significant compared with that of group C,but not compared with that of group A.After receiving IVF-ET,patients of group A showed significantly higher conception rate than groups B and C(62.5% vs 43.6% vs 39.2%).Conclusion Compared with the conventional procedure,laparoscopic "core-pulling" salpingectomy should be recommended for patients with hydrosalpinx intending to receive IVF-ET,which did not interfere with the ov 展开更多
关键词 fertilization in vitro embryo transfer HYDROSALPINX SALPINGECTOMY
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Effects of body mass index on the outcomes of in vitro fertilization in Chinese patients with polycystic ovary syndrome: a retrospective cohort study 被引量:8
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作者 Xiao-ming PAN Zhong LIN +4 位作者 Nan LI Jia-qi WU Dan-qing CHEN Yi-min ZHU Fan QU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2018年第6期490-496,共7页
Objective: To investigate the effects of body mass index(BMI) on the outcomes of in vitro fertilization(IVF) in Chinese patients with polycystic ovary syndrome(PCOS). Methods: In the retrospective cohort study, a tota... Objective: To investigate the effects of body mass index(BMI) on the outcomes of in vitro fertilization(IVF) in Chinese patients with polycystic ovary syndrome(PCOS). Methods: In the retrospective cohort study, a total of 1074 patients with PCOS undergoing IVF between April 2010 and May 2017 in two reproductive medicine centers, respectively in eastern China(Women's Hospital, School of Medicine, Zhejiang University, Zhejiang Province) and in southern China(Maternal and Child Health Care Hospital of Liuzhou, Guangxi Province), were included. The patients were divided into four groups according to the recommended Chinese BMI cut-off points: underweight(BMI<18.5kg/m^2), normal weight(18.5kg/m^2≤BMI<24.0kg/m^2), overweight(24.0kg/m^2≤BMI<28.0kg/m^2), and obese(BMI≥28.0kg/m2). The basic characteristics of the PCOS patients, the details of IVF treatment, and the pregnancy outcomes were collected. Main results: There were no significant differences among the normal weight, overweight, and obese PCOS patients undergoing IVF on the biochemical pregnancy rate, clinical pregnancy rate, miscarriage rate, live birth rate, or term delivery rate(P>0.05), although the overweight and obese PCOS patients required more gonadotropin(Gn)(P<0.001) as well as longer stimulation period(P<0.001), and got less retrieved oocytes(P<0.05) and fertilized oocytes(P<0.05). The underweight PCOS patients required less Gn(P<0.05) and achieved higher live birth rate and term delivery rate(P<0.05), compared with the normal weight PCOS patients. Conclusions: High BMI had no negative effects on the outcomes of IVF in Chinese patients with PCOS; however, the conclusion may seem a little limited due to the retrospective design and the potential bias. 展开更多
关键词 Body mass index In vitro fertilization Polycystic ovary syndrome
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Effects of Acupuncture on Endometrium and Pregnancy Outcomes in Patients with Polycystic Ovarian Syndrome Undergoing in vitro Fertilization-Embryo Transfer:A Randomized Clinical Trial 被引量:7
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作者 WU Jia-man NING Yan +4 位作者 YE Yang-yang LIU Yu-lei TANG Meng HU Shan ZHUO Yuan-yuan 《Chinese Journal of Integrative Medicine》 SCIE CAS CSCD 2022年第8期736-742,共7页
Objective:To observe the effect of acupuncture on endometrium and pregnancy outcomes in patients with polycystic ovary syndrome(PCOS)infertility undergoing in vitro fertilization-embryo transfer(IVF-ET).Methods:Eighty... Objective:To observe the effect of acupuncture on endometrium and pregnancy outcomes in patients with polycystic ovary syndrome(PCOS)infertility undergoing in vitro fertilization-embryo transfer(IVF-ET).Methods:Eighty-three patients were randomly assigned to observation group(40 cases)and control group(43 cases)according to the random numbers generated by SPSS software.The patients of the two groups received GnRH agonist long protocol as a routine treatment.In the observation group,acupuncture was given at two acupoint groups for 30 min once every other day.Group 1 included Guanyuan(CV 4),Qihai(CV 6),Zhongji(CV 3),bilateral acupoints Zigong(EX-CA1).Group 2 included Mingmen(GV 4),Yaoyangguan(GV 3),bilateral Shenshu(BL 23)and Ciliao(BL 32).The two groups of acupoints were used alternately.The whole needling process was performed at the time of ovulation induction until the transplantation day and consisted of3 courses,while the control group did not receive acupuncture interventions.The Gn dosage and Gn stimulation time,endometrial thickness and type(A,B,and C),serum oestradiol(E2)and progesterone(P)levels on the day of injection of human chorionic gonadotropin(hCG),clinical pregnancy rate,as well as live birth rate were observed.Adverse reactions were also be recorded.All patients were followed up for the pregnant rate 14 days after IVF-ET and live birth rate after pregnancy.All adverse reactions(AEs)of acupuncture were recorded during the trial.Ressults:The Gn dosage and Gn stimulation time in the observation group were lower in the observation group than in the control group(P<0.01).The proportion of type A endometrium in the observation group were higher than that in the control group(P<0.05).The serum E2 and P levels on the day of hCG injection was lower and the clinical pregnancy rate was higher in the observation group compared to those in the control group(P<0.05).There was no serious AEs during this trial.Conclusion:Acupuncture can improve the proportion of type A endometrium,regulate the levelse of serum 展开更多
关键词 ACUPUNCTURE in vitro fertilization-embryo transfer polycystic ovarian syndrome ENDOMETRIUM
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Influence of Different Gonadotropin-releasing Hormone Agonist Administration Methods on Pregnancy Outcomes of Patients Undergoing In-vitro Fertilization-embryo Transfer 被引量:8
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作者 Li WU Xin-ling REN +3 位作者 Wen CHEN Bo HUANG Yi-fan ZHOU Lei JIN 《Current Medical Science》 SCIE CAS 2019年第3期437-441,共5页
This study aimed to investigate the effect of different gonadotropin-releasing hormone agonist (GnRH-a) administration methods on pregnancy outcomes of patients undergoing in-vitro fertilization-embryo transfer (IVF-E... This study aimed to investigate the effect of different gonadotropin-releasing hormone agonist (GnRH-a) administration methods on pregnancy outcomes of patients undergoing in-vitro fertilization-embryo transfer (IVF-ET). Clinical data of 5217 patients who underwent IVF-ET were retrospectively analyzed. Patients were divided into the long-acting GnRH-a group (n=1330) and the short-acting GnRH-a group (w=3887) based on their various treatment plans. The clinical and laboratory embryo data and clinical pregnancy outcomes were compared between the two groups. The results showed that there were no significant differences in the age, infertility, primary/secondary infertility rate, IVF rate, body mass index (BMI), antral follicle counting (AFC), folliclestimulating hormone (FSH) level, and the number of transplanted embryos between the two groups (P>0.05). There were no significant differences in the oocyte numbers, M II rate, fertilization rate, cleavage rate and blastocyst formation rate (P>0.05) between the two groups. The gonadotropin (Gn) using days, Gn dose and endometrial thickness were significantly greater in the long-acting GnRH-a group than those in the short-acting GnRH-a group (P<0.01). Additionally, the estradiol (E2) levels, blastocyst freezing rate, embryo utilization rate, transplant cancellation rate and abortion rate were significantly lower in the long-acting GnRH-a group than those in the shortacting GnRH-a group (P<0.01). The clinical pregnancy rate and embryo implantation rate were significantly higher in the long-acting GnRH-a group than in the short-acting GnRH-a group (P<O.Ol). It was concluded that use of long-acting GnRH-a can effectively reduce the transplant cancellation rate and improve the clinical pregnancy rate of the fresh cycle. 展开更多
关键词 gonadotropin-releasing hormone AGONIST LONG-ACTING short-acting in-vitro fertilization-embryo transfer clinical pregnancy rate
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Optimal waiting period for fresh embryo transfer after hysteroscopic adhesiolysis: a retrospective cohort study 被引量:8
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作者 Ke Deng Xiao-Hua Song +4 位作者 Xue-Mei Han Wei-Ya Kong Wei Liu Ting Han Lei Yan 《Chinese Medical Journal》 SCIE CAS CSCD 2019年第19期2333-2339,共7页
Background:Very few studies have been conducted regarding the optimal time interval between hysteroscopic adhesiolysis and the embryo transfer(ET).Investigation of this optimal time may be helpful for assisted reprodu... Background:Very few studies have been conducted regarding the optimal time interval between hysteroscopic adhesiolysis and the embryo transfer(ET).Investigation of this optimal time may be helpful for assisted reproductive technology.Therefore,we investigated effects of the interval between hysteroscopic adhesiolysis and ET upon in vitro fertilization(IVF)cycle outcomes.Methods:Patients were recruited between January 2014 and September 2017 at the Reproductive Hospital Affiliated to Shandong University.Patients who were diagnosed with intra-uterine adhesion(IUA)and underwent hysteroscopic adhesiolysis before fresh IVF-ET or intra-cytoplasmic sperm injection cycles were classified into three groups according to the interval between hysteroscopic adhesiolysis and ET:less than 90 days(Group 1),90 to 180 days(Group 2),and greater than 180 days(Group 3).Baseline characteristics,controlled ovarian stimulation(COS)response,and pregnancy outcomes after ET were compared.Analysis of variance or non-parametric tests were used to test numerical data.The Pearson’s Chi-squared test was used to test categorical data.Results:A total of 312 patients were recruited as follows:112 in Group 1,137 in Group 2,and 63 in Group 3.There were no differences in baseline and COS characteristics among the three groups.The live-birth rate in Group 2(40.1%)was significantly higher than that in Group 1(17.9%;χ^2=14.545,P<0.001).There were no significant differences in the rates of biochemical,ongoing,and clinical pregnancy,and biochemical and clinical pregnancy abortion,as well as stillbirth among the groups.In the mild IUA patients,the live-birth rate was significantly higher in Group 2(42.6%)compared with Group 1(22%;χ^2=8.413,P=0.004).In the moderate IUA patients,Group 2(35.7%)had a higher frequency of live births than Group 1(6.7%;χ^2=8.187,P=0.004).Conclusions:The optimal waiting period for fresh ET after hysteroscopic adhesiolysis was 90 to 180 days in the current study. 展开更多
关键词 Asherman SYNDROME HYSTEROSCOPY In vitro fertilization
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