The enzymatic limited hydrolysis of soy protein isolate (SPI) by Alcalase 0.6?L was carried out with pH-stat method at pH 8.0,50?℃.The effects of the main parameters on the degree of hydrolysis, x , were investigated...The enzymatic limited hydrolysis of soy protein isolate (SPI) by Alcalase 0.6?L was carried out with pH-stat method at pH 8.0,50?℃.The effects of the main parameters on the degree of hydrolysis, x , were investigated and a model based on the experiment results was deduced.The results showed that the substrate and the enzyme-substrate intermediate induced enzyme inhibition,which occurred due to free enzyme attacking the enzyme-substrate complexes.A critical 0 would correspond to a constant 0,which was 96.77?mg·ml -1 under the experiment condition.The kinetic parameters calculated from the experiment data were K m=0.016?mol·L -1 and V max =0.765?mol·min·L -1 .The results showed that x can be regulated to about 6.0 with the mentioned factors in 30?min.The good fitness between predicted and measured results confirmed the usefulness of the model.展开更多
文摘The enzymatic limited hydrolysis of soy protein isolate (SPI) by Alcalase 0.6?L was carried out with pH-stat method at pH 8.0,50?℃.The effects of the main parameters on the degree of hydrolysis, x , were investigated and a model based on the experiment results was deduced.The results showed that the substrate and the enzyme-substrate intermediate induced enzyme inhibition,which occurred due to free enzyme attacking the enzyme-substrate complexes.A critical 0 would correspond to a constant 0,which was 96.77?mg·ml -1 under the experiment condition.The kinetic parameters calculated from the experiment data were K m=0.016?mol·L -1 and V max =0.765?mol·min·L -1 .The results showed that x can be regulated to about 6.0 with the mentioned factors in 30?min.The good fitness between predicted and measured results confirmed the usefulness of the model.
