对广东珠海一养殖池塘中尖吻鲈Lates calcarifer的发病情况进行了调查。调查发现,病鱼体色发黑,剖检可见鳃充血发紫;空肠空胃,肠道微红,脾和肾肿大,有出血点,胆囊充盈,有胆汁渗出,肾发黑,有出血点,其他组织器官无明显变化。从病鱼的鳃...对广东珠海一养殖池塘中尖吻鲈Lates calcarifer的发病情况进行了调查。调查发现,病鱼体色发黑,剖检可见鳃充血发紫;空肠空胃,肠道微红,脾和肾肿大,有出血点,胆囊充盈,有胆汁渗出,肾发黑,有出血点,其他组织器官无明显变化。从病鱼的鳃、肝、肾、肌肉、肠道,和血液中未检查到寄生虫,从肝、脾和肾中未分离到致病菌。参考世界动物卫生组织(Office International des Epi-zooties,OIE)设计引物,提取自然发病鱼各种组织的DNA作为模板,扩增出了预期大小的特异性产物。测序比对显示,扩增的条带的基因序列与真鲷虹彩病毒(Red sea bream iridovirus,RSIV)的基因序列同源性高达98.6%。病理切片显示:肝和脾出现肿大细胞。因此,引起尖吻鲈发病死亡的原因是肿大细胞病毒属虹彩病毒感染。展开更多
To study protective effects of Qingshen Compound on experimental anti-glomerular basement membrane antibody （AGBM-Ab） nephritis model in rats,an experimental Masugi nephritis model was established in Sprague-Dawley...To study protective effects of Qingshen Compound on experimental anti-glomerular basement membrane antibody （AGBM-Ab） nephritis model in rats,an experimental Masugi nephritis model was established in Sprague-Dawley（SD） rats by a single-dose injection of prepared rabbit anti-rat glomerular basement membrane（GBM） serum into sublingual vein and then effects of Qingshen Compound on urine volume,urinary protein level,blood urea nitrogen （BUN）,creatinine （Cr）,albumin （Alb）,triglycerides （TG）,cholesterol （CHO） and histopathological changes were investigated.After successive daily three-dosage oral administration to rat groups （9.4,4.7,2.3 g·kg-1） for 8 weeks,Qingshen Compound was proved effective on increasing urine volume and Alb content,and decreasing levels of urinary protein,BUN,Cr and TG.Furthermore,high and middle dosages （9.4,4.7 g·kg-1） could improve histopathological healing of the model.In summary,Qingshen Compound has shown protective effects on experimental Masugi nephritis model in rats.展开更多
文摘对广东珠海一养殖池塘中尖吻鲈Lates calcarifer的发病情况进行了调查。调查发现,病鱼体色发黑,剖检可见鳃充血发紫;空肠空胃,肠道微红,脾和肾肿大,有出血点,胆囊充盈,有胆汁渗出,肾发黑,有出血点,其他组织器官无明显变化。从病鱼的鳃、肝、肾、肌肉、肠道,和血液中未检查到寄生虫,从肝、脾和肾中未分离到致病菌。参考世界动物卫生组织(Office International des Epi-zooties,OIE)设计引物,提取自然发病鱼各种组织的DNA作为模板,扩增出了预期大小的特异性产物。测序比对显示,扩增的条带的基因序列与真鲷虹彩病毒(Red sea bream iridovirus,RSIV)的基因序列同源性高达98.6%。病理切片显示:肝和脾出现肿大细胞。因此,引起尖吻鲈发病死亡的原因是肿大细胞病毒属虹彩病毒感染。
文摘To study protective effects of Qingshen Compound on experimental anti-glomerular basement membrane antibody （AGBM-Ab） nephritis model in rats,an experimental Masugi nephritis model was established in Sprague-Dawley（SD） rats by a single-dose injection of prepared rabbit anti-rat glomerular basement membrane（GBM） serum into sublingual vein and then effects of Qingshen Compound on urine volume,urinary protein level,blood urea nitrogen （BUN）,creatinine （Cr）,albumin （Alb）,triglycerides （TG）,cholesterol （CHO） and histopathological changes were investigated.After successive daily three-dosage oral administration to rat groups （9.4,4.7,2.3 g·kg-1） for 8 weeks,Qingshen Compound was proved effective on increasing urine volume and Alb content,and decreasing levels of urinary protein,BUN,Cr and TG.Furthermore,high and middle dosages （9.4,4.7 g·kg-1） could improve histopathological healing of the model.In summary,Qingshen Compound has shown protective effects on experimental Masugi nephritis model in rats.
