幽门螺杆菌(Helicobacter pylori,H.pylori 或 Hp)是定植于胃粘膜表面与粘膜层之间的微需氧菌.自1983年 Marshall et al 首次从人胃粘膜中分离出该菌以来,大量研究表明,该菌与慢性胃炎、消化性溃疡、MALT淋巴瘤等慢性胃病密切相关,也与...幽门螺杆菌(Helicobacter pylori,H.pylori 或 Hp)是定植于胃粘膜表面与粘膜层之间的微需氧菌.自1983年 Marshall et al 首次从人胃粘膜中分离出该菌以来,大量研究表明,该菌与慢性胃炎、消化性溃疡、MALT淋巴瘤等慢性胃病密切相关,也与胃癌有一定关系,受到医学界的广泛重视.因此,Hp 与人类免疫缺陷病毒(HIV)和艾滋病的发现成为过去20 a 中最重要的医疗事件.但是 Hp 感染在不同地区、不同国家结果不一;对 Hp的毒力、基因分型、Hp 对宿主的损伤;Hp 相关性疾病的发病机制以及 Hp 对胃癌发生与发展的关系等尚未完全明确.因此,对 Hp 感染的临床意义、治疗与评价也不一致.现就 Hp 感染的诊断与治疗,结合文献进行讨论.旨在有助于临床诊疗和进一步深入开展科研工作,提高对 Hp 感染的认识与处理.展开更多
The most common methods for three-dimensional reconstruction of peripheral nerve fascicles include histological and radiology techniques. Histological techniques have many drawbacks including an enormous manual worklo...The most common methods for three-dimensional reconstruction of peripheral nerve fascicles include histological and radiology techniques. Histological techniques have many drawbacks including an enormous manual workload and poor image registration. Micro-magnetic resonance imaging（Micro-MRI）, an emerging radiology technique, has been used to report results in the brain, liver and tumor tissues. However, micro-MRI usage for obtaining intraneural structures has not been reported. The aim of this study was to present a new imaging method for three-dimensional reconstruction of peripheral nerve fascicles by ~1T micro-MRI. Freshly harvested sciatic nerve samples from an amputated limb were divided into four groups. Two different scanning conditions（Mannerist Solution/GD-DTPA contrast agent, distilled water） were selected, and both T1 and T2 phases programmed for each scanning condition. Three clinical surgeons evaluated the quality of the images via a standardized scale. Moreover, to analyze deformation of the two-dimensional image, the nerve diameter and total area of the micro-MRI images were compared after hematoxylin-eosin staining. The results show that rapid micro-MRI imaging method can be used for three-dimensional reconstruction of the fascicle structure. Nerve sample immersed in contrast agent（Mannerist Solution/GD-DTPA） and scanned in the T1 phase was the best. Moreover, the nerve sample was scanned freshly and can be recycled for other procedures. MRI images show better stability and smaller deformation compared with histological images. In conclusion, micro-MRI provides a feasible and rapid method for three-dimensional reconstruction of peripheral nerve fascicles, which can clearly show the internal structure of the peripheral nerve.展开更多
Background Blepharophimosis ptosis epicanthus inversus syndrome （BPES） is a rare congenital ophthalmic disorder, characterized by congenital eyelid malformation including bilateral ptosis, shortening of the horizont...Background Blepharophimosis ptosis epicanthus inversus syndrome （BPES） is a rare congenital ophthalmic disorder, characterized by congenital eyelid malformation including bilateral ptosis, shortening of the horizontal eyelid fissure, epicanthus inversus, and increased distance between the inner canthi. In this research, we studied the histological structure and ultrastructure of medial canthal ligament of patients with BPES.Methods Thirty patients with BPES who received plastic surgery at the Zhongshan Ophthalmic Center from March 2006 to January 2008 were studied. There were 17 males and 13 females with an average age of （8.73±3.37） years （3-31 years）. The medial canthal ligaments of patients were collected during the plastic surgery to analyze the histological structure by hematoxylin and eosin （HE）, Congo red, van Gieson＇s （VG）, Masson trichrome and aldehyde-fuchsin staining. The ultrastructures of the medial canthal ligaments were also analyzed by scanning electron microscopy （SEM） and transmission electron microscopy （TEM）. Fifteen samples of medial canthal ligament from healthy persons with an average age of （9.02±3.12） years （6-30 years） were collected as a control group.Results Morphological and histological study showed that the medial canthal ligaments of BPES patients were composed of collagen fibers, a few elastic fibers and striated muscles. The collagen fibers assemblies were disorganized and the fibrous connective tissues were undergoing hyaline degeneration. The karyopycnosis of fibroblasts was located among the collagen fibrils and the numbers of fibroblasts were decreased. Ultrastructural study with SEM showed that the collagen fibers were larger than normal, irregular and loose. Parts of the collagen fibers were broken and had a coarse surface. Ultrastructural study with TEM showed that the fibroblasts had less cytoplasm, fewer organelles and the nucleus displayed pyknosis.Conclusions The medial canthal ligament in BPES patients is composed chiefly of col展开更多
文摘幽门螺杆菌(Helicobacter pylori,H.pylori 或 Hp)是定植于胃粘膜表面与粘膜层之间的微需氧菌.自1983年 Marshall et al 首次从人胃粘膜中分离出该菌以来,大量研究表明,该菌与慢性胃炎、消化性溃疡、MALT淋巴瘤等慢性胃病密切相关,也与胃癌有一定关系,受到医学界的广泛重视.因此,Hp 与人类免疫缺陷病毒(HIV)和艾滋病的发现成为过去20 a 中最重要的医疗事件.但是 Hp 感染在不同地区、不同国家结果不一;对 Hp的毒力、基因分型、Hp 对宿主的损伤;Hp 相关性疾病的发病机制以及 Hp 对胃癌发生与发展的关系等尚未完全明确.因此,对 Hp 感染的临床意义、治疗与评价也不一致.现就 Hp 感染的诊断与治疗,结合文献进行讨论.旨在有助于临床诊疗和进一步深入开展科研工作,提高对 Hp 感染的认识与处理.
基金supported by grants from the National Key Research and Development Plan of China,No.31670986(to QTZ)the Science and Technology Project of Guangdong Province of China,No.2014B020227001,2017A050501017(to QTZ)the Science and Technology Project of Guangzhou of China,No.201807010082(to QTZ),201704030041(to JQ)
文摘The most common methods for three-dimensional reconstruction of peripheral nerve fascicles include histological and radiology techniques. Histological techniques have many drawbacks including an enormous manual workload and poor image registration. Micro-magnetic resonance imaging（Micro-MRI）, an emerging radiology technique, has been used to report results in the brain, liver and tumor tissues. However, micro-MRI usage for obtaining intraneural structures has not been reported. The aim of this study was to present a new imaging method for three-dimensional reconstruction of peripheral nerve fascicles by ~1T micro-MRI. Freshly harvested sciatic nerve samples from an amputated limb were divided into four groups. Two different scanning conditions（Mannerist Solution/GD-DTPA contrast agent, distilled water） were selected, and both T1 and T2 phases programmed for each scanning condition. Three clinical surgeons evaluated the quality of the images via a standardized scale. Moreover, to analyze deformation of the two-dimensional image, the nerve diameter and total area of the micro-MRI images were compared after hematoxylin-eosin staining. The results show that rapid micro-MRI imaging method can be used for three-dimensional reconstruction of the fascicle structure. Nerve sample immersed in contrast agent（Mannerist Solution/GD-DTPA） and scanned in the T1 phase was the best. Moreover, the nerve sample was scanned freshly and can be recycled for other procedures. MRI images show better stability and smaller deformation compared with histological images. In conclusion, micro-MRI provides a feasible and rapid method for three-dimensional reconstruction of peripheral nerve fascicles, which can clearly show the internal structure of the peripheral nerve.
文摘Background Blepharophimosis ptosis epicanthus inversus syndrome （BPES） is a rare congenital ophthalmic disorder, characterized by congenital eyelid malformation including bilateral ptosis, shortening of the horizontal eyelid fissure, epicanthus inversus, and increased distance between the inner canthi. In this research, we studied the histological structure and ultrastructure of medial canthal ligament of patients with BPES.Methods Thirty patients with BPES who received plastic surgery at the Zhongshan Ophthalmic Center from March 2006 to January 2008 were studied. There were 17 males and 13 females with an average age of （8.73±3.37） years （3-31 years）. The medial canthal ligaments of patients were collected during the plastic surgery to analyze the histological structure by hematoxylin and eosin （HE）, Congo red, van Gieson＇s （VG）, Masson trichrome and aldehyde-fuchsin staining. The ultrastructures of the medial canthal ligaments were also analyzed by scanning electron microscopy （SEM） and transmission electron microscopy （TEM）. Fifteen samples of medial canthal ligament from healthy persons with an average age of （9.02±3.12） years （6-30 years） were collected as a control group.Results Morphological and histological study showed that the medial canthal ligaments of BPES patients were composed of collagen fibers, a few elastic fibers and striated muscles. The collagen fibers assemblies were disorganized and the fibrous connective tissues were undergoing hyaline degeneration. The karyopycnosis of fibroblasts was located among the collagen fibrils and the numbers of fibroblasts were decreased. Ultrastructural study with SEM showed that the collagen fibers were larger than normal, irregular and loose. Parts of the collagen fibers were broken and had a coarse surface. Ultrastructural study with TEM showed that the fibroblasts had less cytoplasm, fewer organelles and the nucleus displayed pyknosis.Conclusions The medial canthal ligament in BPES patients is composed chiefly of col
