This paper aims at the development of an approach integrating the fuzzy logic strategy for a therapeutic hepatitis C virus dynamics optimal control problem. To test the efficiency of this strategy, the authors propose...This paper aims at the development of an approach integrating the fuzzy logic strategy for a therapeutic hepatitis C virus dynamics optimal control problem. To test the efficiency of this strategy, the authors propose a numerical comparison with the direct method by taking the values of determinant parameters of this disease for people administrating the drugs. The results are in good agreement with experimental data.展开更多
目的观察葛黄颗粒含药血清对乙醇损伤的人肝细胞L-02修复作用,并探讨其可能机制。方法 (1)将L-02细胞分为A、B、C、D、E、F组。除F组外,其余各组均加入乙醇培养基培养24 h制备肝细胞损伤模型,造模后吸走培养液。D、F组加入空白血清(10%...目的观察葛黄颗粒含药血清对乙醇损伤的人肝细胞L-02修复作用,并探讨其可能机制。方法 (1)将L-02细胞分为A、B、C、D、E、F组。除F组外,其余各组均加入乙醇培养基培养24 h制备肝细胞损伤模型,造模后吸走培养液。D、F组加入空白血清(10%正常大鼠血清),A组加入1.25%葛黄颗粒含药血清+8.75%正常大鼠血清,B组加入2.5%葛黄颗粒含药血清+7.5%正常大鼠血清,C组加入5%葛黄颗粒含药血清+5%正常大鼠血清,E组加入5%美他多辛含药血清+5%正常大鼠血清,继续培养6、12、24 h。比较各组细胞上清液谷草转氨酶(AST)、乳酸脱氢酶(LDH)活力。(2)将L-02细胞按2×104个/孔接种于6孔板中,将其分为a、b、c、d组。除d组外,其余各组均加入乙醇制备肝细胞损伤模型,造模后吸走培养液。b、d组加入空白血清,a组加入5%葛黄颗粒含药血清,c组加入5%美他多辛含药血清,继续培养24 h。比较各组丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px)、细胞活性氧(ROS)、线粒体膜电位(MMP)、三磷酸腺苷(ATP)。结果与F组比较,D组各时点AST、LDH升高;与D组比较,B组24 h AST、LDH降低,C、E组12 h和24 h的AST、LDH降低;与A组比较,B组24 h AST降低,C组24 h AST及12 h LDH降低,E组12、24 h AST和LDH降低;与B组比较,C、E组12 h AST、LDH降低;与同组6 h比较,C组24 h AST降低,12、24 h LDH降低;与同组12 h比较,B、C、E组24 h AST降低,C组24 h LDH降低;P均<0.05。与d组比较,b组SOD、GSH-px、MMP、ATP降低,MDA、ROS升高(P均<0.05);与b组比较,a组和c组SOD、GSH-px、MMP、ATP升高,MDA、ROS降低(P均<0.05)。结论葛黄颗粒含药血清对乙醇致L-02细胞损伤有一定修复作用,其机制可能是调整细胞内氧化应激水平和线粒体功能。展开更多
AIM To assess the accuracy of Look-Locker on gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid(GdEOB-DTPA)-enhanced magnetic resonance imaging(MRI) for staging liver fibrosis in chronic hepatitis B/C(CHB/C)....AIM To assess the accuracy of Look-Locker on gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid(GdEOB-DTPA)-enhanced magnetic resonance imaging(MRI) for staging liver fibrosis in chronic hepatitis B/C(CHB/C).METHODS We prospectively included 109 patients with CHB or CHC who underwent a 3.0-Tesla MRI examination, including T1-weighted and Look-Locker sequences for T1 mapping. Hepatocyte fractions(He F) and relaxation time reduction rate(RE) were measured for staging liver fibrosis. A receiver operating characteristic analysis using the area under the receiver operating characteristic curve(AUC) was used to compare thediagnostic performance in predicting liver fibrosis between He F and RE.RESULTS A total of 73 patients had both pathological results and MRI information. The number of patients in each fibrosis stage was evaluated semiquantitatively according to the METAVIR scoring system: F0, n = 23(31.5%); F1, n = 19(26.0%); F2, n = 13(17.8%); F3, n = 6(8.2%), and F4, n = 12(16.4%). He F by EOB enhancement imaging was significantly correlated with fibrosis stage(r =-0.808, P < 0.05). AUC values for diagnosis of any(≥ F1), significant(≥ F2) or advanced(≥ F3) fibrosis, and cirrhosis(F4) using He F were 0.837(0.733-0.913), 0.890(0.795-0.951), 0.957(0.881-0.990), and 0.957(0.882-0.991), respectively. He F measurement was more accurate than use of RE in establishing liver fibrosis staging, suggesting that calculation of He F is a superior noninvasive liver fibrosis staging method.CONCLUSION A T1 mapping-based He F method is an efficient diagnostic tool for the staging of liver fibrosis.展开更多
文摘This paper aims at the development of an approach integrating the fuzzy logic strategy for a therapeutic hepatitis C virus dynamics optimal control problem. To test the efficiency of this strategy, the authors propose a numerical comparison with the direct method by taking the values of determinant parameters of this disease for people administrating the drugs. The results are in good agreement with experimental data.
文摘目的观察葛黄颗粒含药血清对乙醇损伤的人肝细胞L-02修复作用,并探讨其可能机制。方法 (1)将L-02细胞分为A、B、C、D、E、F组。除F组外,其余各组均加入乙醇培养基培养24 h制备肝细胞损伤模型,造模后吸走培养液。D、F组加入空白血清(10%正常大鼠血清),A组加入1.25%葛黄颗粒含药血清+8.75%正常大鼠血清,B组加入2.5%葛黄颗粒含药血清+7.5%正常大鼠血清,C组加入5%葛黄颗粒含药血清+5%正常大鼠血清,E组加入5%美他多辛含药血清+5%正常大鼠血清,继续培养6、12、24 h。比较各组细胞上清液谷草转氨酶(AST)、乳酸脱氢酶(LDH)活力。(2)将L-02细胞按2×104个/孔接种于6孔板中,将其分为a、b、c、d组。除d组外,其余各组均加入乙醇制备肝细胞损伤模型,造模后吸走培养液。b、d组加入空白血清,a组加入5%葛黄颗粒含药血清,c组加入5%美他多辛含药血清,继续培养24 h。比较各组丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px)、细胞活性氧(ROS)、线粒体膜电位(MMP)、三磷酸腺苷(ATP)。结果与F组比较,D组各时点AST、LDH升高;与D组比较,B组24 h AST、LDH降低,C、E组12 h和24 h的AST、LDH降低;与A组比较,B组24 h AST降低,C组24 h AST及12 h LDH降低,E组12、24 h AST和LDH降低;与B组比较,C、E组12 h AST、LDH降低;与同组6 h比较,C组24 h AST降低,12、24 h LDH降低;与同组12 h比较,B、C、E组24 h AST降低,C组24 h LDH降低;P均<0.05。与d组比较,b组SOD、GSH-px、MMP、ATP降低,MDA、ROS升高(P均<0.05);与b组比较,a组和c组SOD、GSH-px、MMP、ATP升高,MDA、ROS降低(P均<0.05)。结论葛黄颗粒含药血清对乙醇致L-02细胞损伤有一定修复作用,其机制可能是调整细胞内氧化应激水平和线粒体功能。
基金Supported by National Natural Science Foundation of China,No.81771893,No.81771802,No.81471718 and No.81401376Outstanding youth Foundation of China Medical University,No.yq20160005
文摘AIM To assess the accuracy of Look-Locker on gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid(GdEOB-DTPA)-enhanced magnetic resonance imaging(MRI) for staging liver fibrosis in chronic hepatitis B/C(CHB/C).METHODS We prospectively included 109 patients with CHB or CHC who underwent a 3.0-Tesla MRI examination, including T1-weighted and Look-Locker sequences for T1 mapping. Hepatocyte fractions(He F) and relaxation time reduction rate(RE) were measured for staging liver fibrosis. A receiver operating characteristic analysis using the area under the receiver operating characteristic curve(AUC) was used to compare thediagnostic performance in predicting liver fibrosis between He F and RE.RESULTS A total of 73 patients had both pathological results and MRI information. The number of patients in each fibrosis stage was evaluated semiquantitatively according to the METAVIR scoring system: F0, n = 23(31.5%); F1, n = 19(26.0%); F2, n = 13(17.8%); F3, n = 6(8.2%), and F4, n = 12(16.4%). He F by EOB enhancement imaging was significantly correlated with fibrosis stage(r =-0.808, P < 0.05). AUC values for diagnosis of any(≥ F1), significant(≥ F2) or advanced(≥ F3) fibrosis, and cirrhosis(F4) using He F were 0.837(0.733-0.913), 0.890(0.795-0.951), 0.957(0.881-0.990), and 0.957(0.882-0.991), respectively. He F measurement was more accurate than use of RE in establishing liver fibrosis staging, suggesting that calculation of He F is a superior noninvasive liver fibrosis staging method.CONCLUSION A T1 mapping-based He F method is an efficient diagnostic tool for the staging of liver fibrosis.
