Heine is an iron-containing tetrapyrrole that plays a critical role in regulating a variety of biological processes including oxy- gen and electron transport, gas sensing, signal transduction, biological clock, and mi...Heine is an iron-containing tetrapyrrole that plays a critical role in regulating a variety of biological processes including oxy- gen and electron transport, gas sensing, signal transduction, biological clock, and microRNA processing. Most metazoan cells synthesize heine via a conserved pathway comprised of eight enzyme-cataiyzed reactions. Heme can also be acquired from food or extracellular environment. Cellular heine homeostasis is maintained through the coordinated regulation of synthesis, transport, and degradation. This review presents the current knowledge of the synthesis and transport of heine in metazoans and highlights recent advances in the regulation of these pathways.展开更多
It aims to analyze the impact heme oxygenase -1 （heme oxygenase 1, HO-1） on regulating factors of human hepatoma cell HepG2＇s cell cycle, through constructing recombinant vector of pcDNA3.1 containing wild-type and...It aims to analyze the impact heme oxygenase -1 （heme oxygenase 1, HO-1） on regulating factors of human hepatoma cell HepG2＇s cell cycle, through constructing recombinant vector of pcDNA3.1 containing wild-type and mutant HO-1 gene （＋）-wtHO-1 and pcDNA3.1 （＋）-mHO-1G143H. By using the method of liposome-mediated, the recombinant vector was transfected hepatoma cell line HepG2. And the transfected one with empty vector was treated as a control group. By the selection of G418, stable expression of wild-type and mutant HO-1 in HepG2 liver cancer cell lines were established. Use the blot of semi-quantitative RT-PCR and Western to test transfected cell lines expressing levels of riO-1 mRNA and protein. As HO-1 expression in stably transfected cell lines altered, we use Western blot to test transfected cell lines P21, P27 protein expression levels. As result shows, we got 1 HO-over-expression of wild-type and mutant in HepG2 cells; wild- type and mutant＇s over expression of HO-1 can induce the expression of tumor suppressor genes p21 and p27.we got the conclusion that HO-l＇s over-expression of tumor suppressor genes p21 and p27 is unrelated to the expression of heme decomposition products. HO-1 may regulate the expression of p21 and p27 through other mechanisms.展开更多
基金supported by the National Natural Science Foundation of China(31371435)the National Key Basic Research Program of China(2015CB150300)+1 种基金the Fundamental Research Funds for the Central Universitiesthe Thousand Youth Talents Program of China
文摘Heine is an iron-containing tetrapyrrole that plays a critical role in regulating a variety of biological processes including oxy- gen and electron transport, gas sensing, signal transduction, biological clock, and microRNA processing. Most metazoan cells synthesize heine via a conserved pathway comprised of eight enzyme-cataiyzed reactions. Heme can also be acquired from food or extracellular environment. Cellular heine homeostasis is maintained through the coordinated regulation of synthesis, transport, and degradation. This review presents the current knowledge of the synthesis and transport of heine in metazoans and highlights recent advances in the regulation of these pathways.
文摘It aims to analyze the impact heme oxygenase -1 （heme oxygenase 1, HO-1） on regulating factors of human hepatoma cell HepG2＇s cell cycle, through constructing recombinant vector of pcDNA3.1 containing wild-type and mutant HO-1 gene （＋）-wtHO-1 and pcDNA3.1 （＋）-mHO-1G143H. By using the method of liposome-mediated, the recombinant vector was transfected hepatoma cell line HepG2. And the transfected one with empty vector was treated as a control group. By the selection of G418, stable expression of wild-type and mutant HO-1 in HepG2 liver cancer cell lines were established. Use the blot of semi-quantitative RT-PCR and Western to test transfected cell lines expressing levels of riO-1 mRNA and protein. As HO-1 expression in stably transfected cell lines altered, we use Western blot to test transfected cell lines P21, P27 protein expression levels. As result shows, we got 1 HO-over-expression of wild-type and mutant in HepG2 cells; wild- type and mutant＇s over expression of HO-1 can induce the expression of tumor suppressor genes p21 and p27.we got the conclusion that HO-l＇s over-expression of tumor suppressor genes p21 and p27 is unrelated to the expression of heme decomposition products. HO-1 may regulate the expression of p21 and p27 through other mechanisms.
