Background Increased reactive oxygen species （ROS） formation, which in turn promotes cardiomyocytes apoptosis, is associated with the pathogenesis and progression of various cardiac diseases such as ischemia and hea...Background Increased reactive oxygen species （ROS） formation, which in turn promotes cardiomyocytes apoptosis, is associated with the pathogenesis and progression of various cardiac diseases such as ischemia and heart failure. Recent studies have shown that over expression of heat shock protein 27 （Hsp27） confers resistance to cardiac ischemia/reperfusion injury. However, not much is known about the regulation of myocyte survival by Hsp27. Methods The rat cardiac cell line H9c2, with a stable overexpression of Hsp27, was established, with empty vector transfected H9c2 cells as controls. Following the cells challenged by Hydrogen Peroxide （H2O2）, lactate dehydrogenase （LDH） release, apoptosis, intracellular ROS, cell morphology, mitochondrial transmembrane potential and the activation of serine/threonine kinase Akt were determined. Results Along with marked suppression of H2O2-induced injury by Hsp27 overexpression in H9c2 cells, ROS generation and the loss of mitochondrial membrane potential were also significantly depressed. Furthermore, augmented Akt activation was observed in Hsp27 overexpressed H9c2 cells following H2O2 exposure. Conclusions Hsp27 inhibits oxidative stress-induced H9c2 damage and inhibition of ROS generation and the augmentation of Akt activation may be involved in the protective signaling.展开更多
BACKGROUND Overexpression of heat shock proteins(HSPs) is associated with several malignancies and contributes to the development, progression, and metastasis of cancer, in addition to the inhibition of cellular death...BACKGROUND Overexpression of heat shock proteins(HSPs) is associated with several malignancies and contributes to the development, progression, and metastasis of cancer, in addition to the inhibition of cellular death. In recent years, there has been active research into using HSP inhibitors in several malignancies. Due to the poor prognosis of esophageal adenocarcinoma(EAC), it would be valuable to find new biomarkers for the development of cancer treatments.AIM To evaluate the expressions of HSP27 and HSP70 and their effect on survival in EAC.METHODS Immunohistochemical analyses and evaluations of HSP27 and HSP70 expression were performed on all available samples from 93 patients diagnosed with EACbetween 1990 and 2007 at two university hospitals. Fifteen cases with Barrett's metaplasia and 5 control cases from the same patient population were included in the analysis. HSP expression was quantitatively assessed and classified as high or low. Kaplan-Meier analyses and Cox regression models adjusting for age and sex as well as tumor site, stage, and grade were used to evaluate the effect on survival.RESULTS Tumor stage and surgical treatment were the main prognostic factors. High HSP27 expression in cancer cases was a strong negative predictive factor, with a mean survival of 23 mo compared to the 49 mo in cases with a low expression(P= 0.018). The results were similar for HSP70, with a poorer survival of 17 mo in cases with high HSP70 expression, in contrast to 40 mo(P = 0.006) in cases with a low expression. A Cox regression survival analysis was performed, adjusting for possible confounding factors, and higher HSP27 and HSP70 expressions remained an independent negative prognostic factor. The HSPs' correlation with survival was not affected by cancer treatments. When the analysis was adjusted for all factors, the odds ratios for HSP27 and HSP70 were 3.3(CI: 1.6–6.6, P =0.001) and 2.2(CI: 1.2–3.9, P = 0.02), respectively.CONCLUSION HSP27 and HSP70 overexpression is associated with poor survival in EAC, whic展开更多
为探究不同包装方式下羊肉冷藏过程中热休克蛋白27(heat shock protein 27,Hsp27)、Hsp70表达量的变化及其与羊肉品质间的关系,以宰后1 h羊背最长肌为研究对象,进行托盘、真空、高氧气调(O_(2)、CO_(2)体积比75∶25)和低氧气调(O_(2)、C...为探究不同包装方式下羊肉冷藏过程中热休克蛋白27(heat shock protein 27,Hsp27)、Hsp70表达量的变化及其与羊肉品质间的关系,以宰后1 h羊背最长肌为研究对象,进行托盘、真空、高氧气调(O_(2)、CO_(2)体积比75∶25)和低氧气调(O_(2)、CO_(2)体积比50∶50)包装,分析Hsp27和Hsp70表达量、pH值、色泽、剪切力和蒸煮损失率的变化,并进行Hsp27、Hsp70表达量与肉品质的相关性分析。结果表明:在冷藏后期(144 h),真空包装处理组的亮度值(L^(*))和红度值(a^(*))明显高于托盘包装和气调包装组,剪切力显著低于托盘包装和气调包装组;随着冷藏时间的延长,不同包装方式羊肉Hsp27相对表达量呈先增加后降低趋势,Hsp70相对表达量呈逐渐上升趋势;Hsp27相对表达量与pH值、剪切力呈正相关,与L^(*)、a^(*)、b^(*)和蒸煮损失率呈负相关;Hsp70相对表达量与pH值、剪切力呈负相关,与L^(*)、a^(*)、b^(*)和蒸煮损失率呈正相关。本研究证明了Hsp27相对表达量下调、Hsp70相对表达量上调可提高羊肉的色泽和嫩度,且真空包装处理组羊肉保鲜效果最好。因此,Hsp27、Hsp70可作为羊肉的生物标志物。展开更多
为研究藏羊肉宰后成熟过程中热休克蛋白27(heat shock protein 27,Hsp27)对肌原纤维蛋白降解特性及细胞凋亡酶活力的影响,以经Hsp27抑制剂KRIBB3处理的藏羊肉为研究对象,测定藏羊肉在宰后成熟过程中Caspase-3、Caspase-9活力变化以及肌...为研究藏羊肉宰后成熟过程中热休克蛋白27(heat shock protein 27,Hsp27)对肌原纤维蛋白降解特性及细胞凋亡酶活力的影响,以经Hsp27抑制剂KRIBB3处理的藏羊肉为研究对象,测定藏羊肉在宰后成熟过程中Caspase-3、Caspase-9活力变化以及肌原纤维蛋白的溶解性和降解特性,并通过体外实验进一步研究Hsp27对μ-钙激活酶和Caspase-3降解肌原纤维蛋白的影响。结果表明:Hsp27能显著抑制Caspase-3、Caspase-9的活力(P<0.05),并能够抑制肌原纤维小片化的发生以及肌间线蛋白和肌钙蛋白-T的降解;体外实验发现,Hsp27能够抑制μ-钙激活酶和Caspase-3对肌原纤维蛋白的降解。展开更多
基金This work was supported by a grant from Jiangsu"135"Key Medical Project of China(No.135-14)
文摘Background Increased reactive oxygen species （ROS） formation, which in turn promotes cardiomyocytes apoptosis, is associated with the pathogenesis and progression of various cardiac diseases such as ischemia and heart failure. Recent studies have shown that over expression of heat shock protein 27 （Hsp27） confers resistance to cardiac ischemia/reperfusion injury. However, not much is known about the regulation of myocyte survival by Hsp27. Methods The rat cardiac cell line H9c2, with a stable overexpression of Hsp27, was established, with empty vector transfected H9c2 cells as controls. Following the cells challenged by Hydrogen Peroxide （H2O2）, lactate dehydrogenase （LDH） release, apoptosis, intracellular ROS, cell morphology, mitochondrial transmembrane potential and the activation of serine/threonine kinase Akt were determined. Results Along with marked suppression of H2O2-induced injury by Hsp27 overexpression in H9c2 cells, ROS generation and the loss of mitochondrial membrane potential were also significantly depressed. Furthermore, augmented Akt activation was observed in Hsp27 overexpressed H9c2 cells following H2O2 exposure. Conclusions Hsp27 inhibits oxidative stress-induced H9c2 damage and inhibition of ROS generation and the augmentation of Akt activation may be involved in the protective signaling.
文摘BACKGROUND Overexpression of heat shock proteins(HSPs) is associated with several malignancies and contributes to the development, progression, and metastasis of cancer, in addition to the inhibition of cellular death. In recent years, there has been active research into using HSP inhibitors in several malignancies. Due to the poor prognosis of esophageal adenocarcinoma(EAC), it would be valuable to find new biomarkers for the development of cancer treatments.AIM To evaluate the expressions of HSP27 and HSP70 and their effect on survival in EAC.METHODS Immunohistochemical analyses and evaluations of HSP27 and HSP70 expression were performed on all available samples from 93 patients diagnosed with EACbetween 1990 and 2007 at two university hospitals. Fifteen cases with Barrett's metaplasia and 5 control cases from the same patient population were included in the analysis. HSP expression was quantitatively assessed and classified as high or low. Kaplan-Meier analyses and Cox regression models adjusting for age and sex as well as tumor site, stage, and grade were used to evaluate the effect on survival.RESULTS Tumor stage and surgical treatment were the main prognostic factors. High HSP27 expression in cancer cases was a strong negative predictive factor, with a mean survival of 23 mo compared to the 49 mo in cases with a low expression(P= 0.018). The results were similar for HSP70, with a poorer survival of 17 mo in cases with high HSP70 expression, in contrast to 40 mo(P = 0.006) in cases with a low expression. A Cox regression survival analysis was performed, adjusting for possible confounding factors, and higher HSP27 and HSP70 expressions remained an independent negative prognostic factor. The HSPs' correlation with survival was not affected by cancer treatments. When the analysis was adjusted for all factors, the odds ratios for HSP27 and HSP70 were 3.3(CI: 1.6–6.6, P =0.001) and 2.2(CI: 1.2–3.9, P = 0.02), respectively.CONCLUSION HSP27 and HSP70 overexpression is associated with poor survival in EAC, whic
文摘为研究藏羊肉宰后成熟过程中热休克蛋白27(heat shock protein 27,Hsp27)对肌原纤维蛋白降解特性及细胞凋亡酶活力的影响,以经Hsp27抑制剂KRIBB3处理的藏羊肉为研究对象,测定藏羊肉在宰后成熟过程中Caspase-3、Caspase-9活力变化以及肌原纤维蛋白的溶解性和降解特性,并通过体外实验进一步研究Hsp27对μ-钙激活酶和Caspase-3降解肌原纤维蛋白的影响。结果表明:Hsp27能显著抑制Caspase-3、Caspase-9的活力(P<0.05),并能够抑制肌原纤维小片化的发生以及肌间线蛋白和肌钙蛋白-T的降解;体外实验发现,Hsp27能够抑制μ-钙激活酶和Caspase-3对肌原纤维蛋白的降解。
