AIM:To investigate the effects of vialinin A on viability of human retinal endothelial cells(HRECs)under high glucose condition and its potential mechanism.METHODS:The HRECs were divided into four groups:normal glucos...AIM:To investigate the effects of vialinin A on viability of human retinal endothelial cells(HRECs)under high glucose condition and its potential mechanism.METHODS:The HRECs were divided into four groups:normal glucose control group(NG,5 mmol/L D-glucose),high glucose group(HG,30 mmol/L D-glucose),HG+1μmol/L vialinin A group,and HG+5μmol/L vialinin A group.The cell viabilities were measured with cell counting kit-8(CCK-8)assay for proliferation,with scratch assay for migration,and tube formation,for evaluation of the impact of vialinin A on cellular behaviour.Real-time PCR and Western blotting were used to determine the expression level of vascular endothelial growth factor(VEGF).RESULTS:The proliferative capacity and migration of HRECs was reduced by 5μmol/L vialinin A in high glucose environment(both P<0.05).Vialinin A also inhibited highglucose-induced tube formation of HRECs.The expression level of VEGF and PI3K in HRECs was also significantly decreased by vialinin A(P<0.05).CONCLUSION:Vialinin A inhibits the cell viability of HRECs.It may serve as a potential target for anti-angiogenic therapy.展开更多
Fenneropenaeus chinensis is confined to the Yellow Sea and Bohai Sea in China and the West Coast of the Korean Peninsula. Intra- and in- tercross populations were produced between Rushany (YP) and Korean (KN) populati...Fenneropenaeus chinensis is confined to the Yellow Sea and Bohai Sea in China and the West Coast of the Korean Peninsula. Intra- and in- tercross populations were produced between Rushany (YP) and Korean (KN) populations. Seven traits were recorded. The heterosis of hybrids was computed and comparison between treatments was performed by ANOVA. At the fourth month after post-larvae, six indexes of growth trait and viability showed a range of heterosis, ranging from 0.514% to 14.950%. At the fifth month after post-larvae, six in- dexes of growth trait and viability ranged from -9.000% to 19.090%. The negative heterosis was observed in CL, HST and viability. The heterosis of KN♀×YP♂ tended generally to increase as the age of the Chinese shrimp increased while the heterosis of YP♀×KN♂ tended to decrease. The results indicated that the viability of reciprocal hybrids were not sig- nificantly different (P>0.05) from their parents during the experiment. The result of ANOVA indicated that the F1 hybrids were significantly different (P<0.05) in WST and TW at the fourth month. The multiple comparisons of LSD test indicated that the hybrids of YP♀×KN♂ were significantly different (P<0.05) from their parents in TW. The hybrids of YP♀×KN♂ were significantly different (P<0.05) from the other three combinations in WST. At the fifth months, the F1 hy- brids had significant difference (P<0.05) in body weight while other traits showed no significant dif-ferences (P>0.05) from their parents. The multiple comparisons of LSD test indicated that the hybrids of KN♀×YP♂ were significantly different (P<0.05) from the KN parents in TW. The results indicate that in experimental conditions, the F1 hybrids created from two populations of Chinese shrimp showed a certain level of heterosis for growth performance and viability. The crossing scheme may improve growth perform- ance and viability in Chinese shrimp, but the im- provement may be limited because effective cross- breeding requires the maintenance of pure, pref展开更多
To achieve a good production of a crop, it is essential to know the ability of the species to successfully complete two critical stages in the life cycle such as germination and seedling establishment. In this paper w...To achieve a good production of a crop, it is essential to know the ability of the species to successfully complete two critical stages in the life cycle such as germination and seedling establishment. In this paper we study in comparative form structure of the seed, the importance of tegument in dormancy, the effect of accelerated aging on seed germination and viability, and the early and late growth in J. curcas and J. macrocarpa. External morphology of the seeds allow difference and internally also the embryos show evident differences. J. macrocarpa germination is around 0% - 4%. The total removal of tegument showed a 50% increase and the other treatments between 0% - 10%. Aging accelerated by Tetrazolium test allowed a comparative analysis of VP and GP. J. curcas maintains both to 96 h, while J. macrocarpa the seed viability is registered along the all treatment. J. macrocarpa seeds have less synchronicity than those of J. curcas. ABA and JAs were detected in tegument of J. macrocarpa and J. curcas seeds. JA could have a roll in inhibition of germination of J. macrocarpa seeds. Early and late growth, FW and DW of root, hypocotyl, epicotyl and leaf of J. curcas were significantly different in both species.展开更多
This study determined the effects of selenium on the growth of Fusorium strains and the effects of products extracted from the fungal cultures on relevant indicators of chondrocytes injury.
Fenneropenaeus chinensis distributed in the Yellow Sea and Bohai Sea of China and the west coast of the Korean Peninsula. Different geographical populations represent potentially different genetic resources. To learn ...Fenneropenaeus chinensis distributed in the Yellow Sea and Bohai Sea of China and the west coast of the Korean Peninsula. Different geographical populations represent potentially different genetic resources. To learn further the characteristics of different geographical population, crosses among two wild and three farmed populations were produced. The two wild populations were from the Yellow Sea and Bohai Sea （WYP）, and the west coast of the Korean Peninsula and coast （WKN）. The three farmed populations included the offspring of first generation of wild shrimp from coast in Korea （FKN）, the Huang Hai （the Yellow Sea in Chinese） No.1 （HH1）, and JK98. The phenotypes growth and survival rates of these populations were compared to confirm the feasibility for crossbreeding. The body length （BL）, carapace length （CL）. carapace width （CW）, height of the second and third abdominal segment （HST）, width of the second and third abdominal segment （WST）, length of the first abdominal segment （LF）, length of the last abdominal segment （LL）, live body weight （BW）, and survival rate were measured, Different combinations were statistically performed with ANOVA and Duncan＇s Multiple Range Test. The results show that the survival rate of JK98（♀）×WKN（♂） was the highest, followed by WYP（♀）×WKN（♂）, FKN（♀）×WYP（♂）, FKN（♀）×HHI（♂） and WYP（♀）×FKN（♂）; the body weight of FKN（♀）×HHI（♂） was the highest, followed by FKN（♀）×WYP（♂）, WYP（♀）×WKN（♂）, WYP（♀）×FKN（♂） and JK98（♀）×WKN（♂）; the total length had the same ranking as the body weight. All growth traits in hybrids JK98（♀）×WKN（♂） were the lowest among all combinations. F1 hybrids had significant difference （P〈0.05） in BL, CL, HST, LL, and BW; and insignificant difference （P〉0.05） in other growth traits and survival rate. The results of Duncan＇s Multiple Range Test are that BL and CL of J展开更多
High glucose affects primary afferent neurons in dorsal root ganglia by inhibiting neurite elongation,causing oxidative stress,and inducing neuronal apoptosis and mitochondrial dysfunction,which finally result in neur...High glucose affects primary afferent neurons in dorsal root ganglia by inhibiting neurite elongation,causing oxidative stress,and inducing neuronal apoptosis and mitochondrial dysfunction,which finally result in neuronal damage.Proanthocyanidin,a potent antioxidant,has been shown to have neuroprotective effects.Proanthocyanidin B2 is a common dimer of oligomeric proanthocyanidins.To date,no studies have reported the neuroprotective effects of proanthocyanidin B2 against high-glucose-related neurotoxicity in dorsal root ganglion neurons.In this study,10 μg/m L proanthocyanidin B2 was used to investigate its effect on 45 m M high-glucose-cultured dorsal root ganglion neurons.We observed that challenge with high levels of glucose increased neuronal reactive oxygen species and promoted apoptosis,decreased cell viability,inhibited outgrowth of neurites,and decreased growth-associated protein 43 protein and m RNA levels.Proanthocyanidin B2 administration reversed the neurotoxic effects caused by glucose challenge.Blockage of the phosphatidylinositol 3 kinase/Akt signaling pathway with 10 μM LY294002 eliminated the protective effects of proanthocyanidin B2.Therefore,proanthocyanidin B2 might be a potential novel agent for the treatment of peripheral diabetic neuropathy.展开更多
基金Supported by the National Natural Science Foundation of China(No.81970830)Jiangsu Provincial Medical Innovation Team(No.CXTDA2017039).
文摘AIM:To investigate the effects of vialinin A on viability of human retinal endothelial cells(HRECs)under high glucose condition and its potential mechanism.METHODS:The HRECs were divided into four groups:normal glucose control group(NG,5 mmol/L D-glucose),high glucose group(HG,30 mmol/L D-glucose),HG+1μmol/L vialinin A group,and HG+5μmol/L vialinin A group.The cell viabilities were measured with cell counting kit-8(CCK-8)assay for proliferation,with scratch assay for migration,and tube formation,for evaluation of the impact of vialinin A on cellular behaviour.Real-time PCR and Western blotting were used to determine the expression level of vascular endothelial growth factor(VEGF).RESULTS:The proliferative capacity and migration of HRECs was reduced by 5μmol/L vialinin A in high glucose environment(both P<0.05).Vialinin A also inhibited highglucose-induced tube formation of HRECs.The expression level of VEGF and PI3K in HRECs was also significantly decreased by vialinin A(P<0.05).CONCLUSION:Vialinin A inhibits the cell viability of HRECs.It may serve as a potential target for anti-angiogenic therapy.
文摘Fenneropenaeus chinensis is confined to the Yellow Sea and Bohai Sea in China and the West Coast of the Korean Peninsula. Intra- and in- tercross populations were produced between Rushany (YP) and Korean (KN) populations. Seven traits were recorded. The heterosis of hybrids was computed and comparison between treatments was performed by ANOVA. At the fourth month after post-larvae, six indexes of growth trait and viability showed a range of heterosis, ranging from 0.514% to 14.950%. At the fifth month after post-larvae, six in- dexes of growth trait and viability ranged from -9.000% to 19.090%. The negative heterosis was observed in CL, HST and viability. The heterosis of KN♀×YP♂ tended generally to increase as the age of the Chinese shrimp increased while the heterosis of YP♀×KN♂ tended to decrease. The results indicated that the viability of reciprocal hybrids were not sig- nificantly different (P>0.05) from their parents during the experiment. The result of ANOVA indicated that the F1 hybrids were significantly different (P<0.05) in WST and TW at the fourth month. The multiple comparisons of LSD test indicated that the hybrids of YP♀×KN♂ were significantly different (P<0.05) from their parents in TW. The hybrids of YP♀×KN♂ were significantly different (P<0.05) from the other three combinations in WST. At the fifth months, the F1 hy- brids had significant difference (P<0.05) in body weight while other traits showed no significant dif-ferences (P>0.05) from their parents. The multiple comparisons of LSD test indicated that the hybrids of KN♀×YP♂ were significantly different (P<0.05) from the KN parents in TW. The results indicate that in experimental conditions, the F1 hybrids created from two populations of Chinese shrimp showed a certain level of heterosis for growth performance and viability. The crossing scheme may improve growth perform- ance and viability in Chinese shrimp, but the im- provement may be limited because effective cross- breeding requires the maintenance of pure, pref
文摘To achieve a good production of a crop, it is essential to know the ability of the species to successfully complete two critical stages in the life cycle such as germination and seedling establishment. In this paper we study in comparative form structure of the seed, the importance of tegument in dormancy, the effect of accelerated aging on seed germination and viability, and the early and late growth in J. curcas and J. macrocarpa. External morphology of the seeds allow difference and internally also the embryos show evident differences. J. macrocarpa germination is around 0% - 4%. The total removal of tegument showed a 50% increase and the other treatments between 0% - 10%. Aging accelerated by Tetrazolium test allowed a comparative analysis of VP and GP. J. curcas maintains both to 96 h, while J. macrocarpa the seed viability is registered along the all treatment. J. macrocarpa seeds have less synchronicity than those of J. curcas. ABA and JAs were detected in tegument of J. macrocarpa and J. curcas seeds. JA could have a roll in inhibition of germination of J. macrocarpa seeds. Early and late growth, FW and DW of root, hypocotyl, epicotyl and leaf of J. curcas were significantly different in both species.
基金funded by the Natural Science Basic Research Plan of Shaanxi Province,China(2014JM4170)the Department of disease control of Shaanxi Health and Family Planning Commission,China(2010/2012)
文摘This study determined the effects of selenium on the growth of Fusorium strains and the effects of products extracted from the fungal cultures on relevant indicators of chondrocytes injury.
基金Supported by the cooperation project between the National Fisheries Technical Extension Center of China,Yellow Sea Fisheries Research Institute of China,and Akvaforsk Genetics Center AS of Norway (2006-G55(B))
文摘Fenneropenaeus chinensis distributed in the Yellow Sea and Bohai Sea of China and the west coast of the Korean Peninsula. Different geographical populations represent potentially different genetic resources. To learn further the characteristics of different geographical population, crosses among two wild and three farmed populations were produced. The two wild populations were from the Yellow Sea and Bohai Sea （WYP）, and the west coast of the Korean Peninsula and coast （WKN）. The three farmed populations included the offspring of first generation of wild shrimp from coast in Korea （FKN）, the Huang Hai （the Yellow Sea in Chinese） No.1 （HH1）, and JK98. The phenotypes growth and survival rates of these populations were compared to confirm the feasibility for crossbreeding. The body length （BL）, carapace length （CL）. carapace width （CW）, height of the second and third abdominal segment （HST）, width of the second and third abdominal segment （WST）, length of the first abdominal segment （LF）, length of the last abdominal segment （LL）, live body weight （BW）, and survival rate were measured, Different combinations were statistically performed with ANOVA and Duncan＇s Multiple Range Test. The results show that the survival rate of JK98（♀）×WKN（♂） was the highest, followed by WYP（♀）×WKN（♂）, FKN（♀）×WYP（♂）, FKN（♀）×HHI（♂） and WYP（♀）×FKN（♂）; the body weight of FKN（♀）×HHI（♂） was the highest, followed by FKN（♀）×WYP（♂）, WYP（♀）×WKN（♂）, WYP（♀）×FKN（♂） and JK98（♀）×WKN（♂）; the total length had the same ranking as the body weight. All growth traits in hybrids JK98（♀）×WKN（♂） were the lowest among all combinations. F1 hybrids had significant difference （P〈0.05） in BL, CL, HST, LL, and BW; and insignificant difference （P〉0.05） in other growth traits and survival rate. The results of Duncan＇s Multiple Range Test are that BL and CL of J
基金supported by the National Natural Science Foundation of China,No.81501935the Shandong Provincial Natural Science Foundation of China,No.ZR2014HQ065
文摘High glucose affects primary afferent neurons in dorsal root ganglia by inhibiting neurite elongation,causing oxidative stress,and inducing neuronal apoptosis and mitochondrial dysfunction,which finally result in neuronal damage.Proanthocyanidin,a potent antioxidant,has been shown to have neuroprotective effects.Proanthocyanidin B2 is a common dimer of oligomeric proanthocyanidins.To date,no studies have reported the neuroprotective effects of proanthocyanidin B2 against high-glucose-related neurotoxicity in dorsal root ganglion neurons.In this study,10 μg/m L proanthocyanidin B2 was used to investigate its effect on 45 m M high-glucose-cultured dorsal root ganglion neurons.We observed that challenge with high levels of glucose increased neuronal reactive oxygen species and promoted apoptosis,decreased cell viability,inhibited outgrowth of neurites,and decreased growth-associated protein 43 protein and m RNA levels.Proanthocyanidin B2 administration reversed the neurotoxic effects caused by glucose challenge.Blockage of the phosphatidylinositol 3 kinase/Akt signaling pathway with 10 μM LY294002 eliminated the protective effects of proanthocyanidin B2.Therefore,proanthocyanidin B2 might be a potential novel agent for the treatment of peripheral diabetic neuropathy.
