This paper investigated the effects of five kinds of Au surfaces terminated with and without functional groups on the crystallization of ferric oxides/oxyhydroxides in the suspension condition. Self-assembled monolaye...This paper investigated the effects of five kinds of Au surfaces terminated with and without functional groups on the crystallization of ferric oxides/oxyhydroxides in the suspension condition. Self-assembled monolayers （SAMs） were used to create hydroxyl （-OH）, carboxyl （-COOH）, amine （-NH2） and methyl （-CH3） functionalized surfaces, which proved to be of the same surface density. The immersion time of substrates in the Fe（OH）3 suspension was divided into two time portions. During the first period of 2 h, few ferric oxide/oxyhydroxide was deposited except that E-Fe203 was detected on -NH2 surface. Crystallization for 10h evidenced more kinds of iron compounds on the functional surfaces. Goethite and maghemite were noticed on four functional surfaces, and maghemite also grew on Au surface. Deposition of ^-Fe203 was found on -OH surface, while the growth of orthorhombic and hexagon FeOOH were indicated on -NH2 surface. Considering the wide existence of iron compounds in nature, our investigation is a precedent work to the study of iron biomineralization in the suspension area.展开更多
文摘This paper investigated the effects of five kinds of Au surfaces terminated with and without functional groups on the crystallization of ferric oxides/oxyhydroxides in the suspension condition. Self-assembled monolayers （SAMs） were used to create hydroxyl （-OH）, carboxyl （-COOH）, amine （-NH2） and methyl （-CH3） functionalized surfaces, which proved to be of the same surface density. The immersion time of substrates in the Fe（OH）3 suspension was divided into two time portions. During the first period of 2 h, few ferric oxide/oxyhydroxide was deposited except that E-Fe203 was detected on -NH2 surface. Crystallization for 10h evidenced more kinds of iron compounds on the functional surfaces. Goethite and maghemite were noticed on four functional surfaces, and maghemite also grew on Au surface. Deposition of ^-Fe203 was found on -OH surface, while the growth of orthorhombic and hexagon FeOOH were indicated on -NH2 surface. Considering the wide existence of iron compounds in nature, our investigation is a precedent work to the study of iron biomineralization in the suspension area.
文摘为了建立一种快速、特异、灵敏的检测I群禽腺病毒的液相基因芯片方法。根据Hexon基因序列设计特异引物,上游引物5'端加TAG序列,下游引物5'端加生物素,进行PCR扩增,将扩增产物与链霉素亲和蛋白、磁珠37℃孵育30 min,通过Luminex200仪器分析。用所建立的液相基因芯片方法进行特异性、灵敏度、重复性及病料样品的检测。结果该方法的特异性强;检测的敏感性可达1×102copies/μL;批内批间的变异系数都在5%以下;检测结果与SYBR Green I荧光PCR方法 100%相符。表明建立的液相基因芯片方法特异性好、灵敏高、重复性好,可用于I群禽腺病毒的检测。
