The use of Chinese herbal medicines can replace antibiotics that cause drug-resistance problems,which are currently necessary for disease control.In this paper,a traditional Chinese medicine compound named Ephedra hou...The use of Chinese herbal medicines can replace antibiotics that cause drug-resistance problems,which are currently necessary for disease control.In this paper,a traditional Chinese medicine compound named Ephedra houttuynia granule for the treatment of Mycoplasma galliscepticum(MG)infection was prepared.Furthermore,its action mechanism was explored through network pharmacology.The optimal extraction and granulation processes of the compound were determined by high performance liquid chromatography(HPLC)method and L9 orthogonal test,and in the treatment experiment,Ephedra houttuynia granule showed a significant therapeutic effect on MG infection.In the study of network pharmacology,the results showed that the core targets of Ephedra houttuynia granule against MG infection were vascular endothelial growth factor(VEGFA),fos proto-oncogene(FOS),prepro-coagulation factor II(F2),etc.,the gene ontology/kyoto encyclopedia of genes and genomes(GO/KEGG)analysis results indicated that the signaling pathways of neuroactive ligand receptor interaction,cAMP,IL-17,T cell receptor,and tumor necrosis factor(TNF)might involve in anti-MG infection.In conclusion,this study would provide a new idea for elucidating the action mechanism of other diseases in veterinary clinic,which had a certain guiding significance.展开更多
目的:对运脾颗粒成型工艺、临界相对湿度(CRH)以及质量标准的含测提取方法进行考察研究。方法:以颗粒的成型性、休止角、溶化性以及吸湿性作为测评指标,单因素试验法考察乙醇不同浓度、不同用量对其成型性综合测评指标的影响;配置不同...目的:对运脾颗粒成型工艺、临界相对湿度(CRH)以及质量标准的含测提取方法进行考察研究。方法:以颗粒的成型性、休止角、溶化性以及吸湿性作为测评指标,单因素试验法考察乙醇不同浓度、不同用量对其成型性综合测评指标的影响;配置不同浓度的硫酸溶液,先测定复方运脾颗粒的吸湿率并绘制吸湿平衡图,再考察复方运脾颗粒的临界相对湿度(CRH);比较提取方法、提取时间、溶媒容量对运脾颗粒中柚皮苷与橙皮苷的含量。结果:在60%的相对湿度下,以每5g浸膏粉加阿斯巴甜0.025g,加70%的乙醇0.4m L为制粒工艺条件;运脾颗粒的临界相对湿度60%;优化的提取工艺条件为称取本品颗粒0.5g,精密称定,精密加入甲醇25 m L,称定重量,水浴回流60 min。结论:该制粒工艺经济、稳定、可行,可以作为运脾颗粒制粒工艺条件;运脾颗粒制粒、分装及贮存应控制在60%的临界相对湿度以下;优化提取的工艺条件简便、稳定可行。展开更多
基金the National Natural Science Foundation of China(32273062,31973005)。
文摘The use of Chinese herbal medicines can replace antibiotics that cause drug-resistance problems,which are currently necessary for disease control.In this paper,a traditional Chinese medicine compound named Ephedra houttuynia granule for the treatment of Mycoplasma galliscepticum(MG)infection was prepared.Furthermore,its action mechanism was explored through network pharmacology.The optimal extraction and granulation processes of the compound were determined by high performance liquid chromatography(HPLC)method and L9 orthogonal test,and in the treatment experiment,Ephedra houttuynia granule showed a significant therapeutic effect on MG infection.In the study of network pharmacology,the results showed that the core targets of Ephedra houttuynia granule against MG infection were vascular endothelial growth factor(VEGFA),fos proto-oncogene(FOS),prepro-coagulation factor II(F2),etc.,the gene ontology/kyoto encyclopedia of genes and genomes(GO/KEGG)analysis results indicated that the signaling pathways of neuroactive ligand receptor interaction,cAMP,IL-17,T cell receptor,and tumor necrosis factor(TNF)might involve in anti-MG infection.In conclusion,this study would provide a new idea for elucidating the action mechanism of other diseases in veterinary clinic,which had a certain guiding significance.
文摘目的:对运脾颗粒成型工艺、临界相对湿度(CRH)以及质量标准的含测提取方法进行考察研究。方法:以颗粒的成型性、休止角、溶化性以及吸湿性作为测评指标,单因素试验法考察乙醇不同浓度、不同用量对其成型性综合测评指标的影响;配置不同浓度的硫酸溶液,先测定复方运脾颗粒的吸湿率并绘制吸湿平衡图,再考察复方运脾颗粒的临界相对湿度(CRH);比较提取方法、提取时间、溶媒容量对运脾颗粒中柚皮苷与橙皮苷的含量。结果:在60%的相对湿度下,以每5g浸膏粉加阿斯巴甜0.025g,加70%的乙醇0.4m L为制粒工艺条件;运脾颗粒的临界相对湿度60%;优化的提取工艺条件为称取本品颗粒0.5g,精密称定,精密加入甲醇25 m L,称定重量,水浴回流60 min。结论:该制粒工艺经济、稳定、可行,可以作为运脾颗粒制粒工艺条件;运脾颗粒制粒、分装及贮存应控制在60%的临界相对湿度以下;优化提取的工艺条件简便、稳定可行。
