Aim To study the metabolite of stilbene glucoside in the Chinese traditional medicine Polygonum multiflornm in mice and elucidate its chemical structure by liquid chromatography tandem-mass spectrometry. Methods The s...Aim To study the metabolite of stilbene glucoside in the Chinese traditional medicine Polygonum multiflornm in mice and elucidate its chemical structure by liquid chromatography tandem-mass spectrometry. Methods The stilbene glucoside was injected into the tail vein of mice. Blood samples were collected from artery in the eyepit. The methanol-protein-precipitated plasma sample was introduced into the liquid chromatography-tandem mass spectrometer directly. The analytical column was C_ 18 column (250 mm×4.6 mm ID, 5 μm). The mobile phase consisted of acetonitrile-methanol-water-formic acid (15∶18∶66∶1) for ES +, acetonitrile-methanol-water (15∶18∶67) for ES -. The UV detection wavelength was set at 320 nm. The mass ion source type was ESI. HV capillary was 3 kV. The dry gas was nitrogen gas and the flow rate was set at 318 L·h -1 . The ion source temperature was 150 ℃. Results The stilbene glucoside and its metabolite were separated completely under the chromatography condition. The ions at m/z 600 and m/z 605 were detected under positive ion polarity while the ions at m/z 581 and m/z 402 were detected under negative ion polarity. Conclusion It was proposed that the metabolite of stilbene glucoside injected in vein was its glucuronide conjugate.展开更多
A new andrographolide metabolite 1 was isolated from human urine samples after oral administration. The structure was determined to be 3-carbonylandrographolide-19-O-β-D-glu- curonide on the basis of chemical evidenc...A new andrographolide metabolite 1 was isolated from human urine samples after oral administration. The structure was determined to be 3-carbonylandrographolide-19-O-β-D-glu- curonide on the basis of chemical evidences and spectral analysis, especially by 2D-NMR techni- ques.展开更多
目的：通过研究巴西苏木素在大鼠体内的代谢产物,探析其代谢途径。方法：采用UPLC-Q-TOF-MS/MS对灌胃巴西苏木素（30 mg·kg^-1）后大鼠的生物样品（粪便、尿液、血浆、胆汁）进行分析,流动相0.1%甲酸水溶液-乙腈梯度洗脱,流速0.4 m...目的：通过研究巴西苏木素在大鼠体内的代谢产物,探析其代谢途径。方法：采用UPLC-Q-TOF-MS/MS对灌胃巴西苏木素（30 mg·kg^-1）后大鼠的生物样品（粪便、尿液、血浆、胆汁）进行分析,流动相0.1%甲酸水溶液-乙腈梯度洗脱,流速0.4 m L min^-1,电喷雾离子源,负离子模式,信息依赖性采集模式进行检测,离子喷雾电压-4.5 k V,离子源温度500℃,碰撞电压100 e V,碰撞能量-35 e V,锥孔气流50 L·h^-1,m/z 100-1 100,使用Peak View 1.2软件进行数据处理,提取给药样品的总离子流图谱与空白生物样品对比,通过保留时间、精确相对分子质量及二级质谱的比较与分析,筛选并鉴定其可能的代谢产物。结果：共检测出23个代谢产物,这23个代谢产物是巴西苏木素在大鼠体内甲基化、脱氧、缩氢、羧酸化、水合作用、硫酸化、葡糖糖醛酸化后产生的,其中以硫酸化、葡糖糖醛酸化为主。结论：本实验首次对巴西苏木素在大鼠体内的代谢情况进行研究,能够为该药物的药效学和药理学研究提供一定的物质基础。展开更多
文摘Aim To study the metabolite of stilbene glucoside in the Chinese traditional medicine Polygonum multiflornm in mice and elucidate its chemical structure by liquid chromatography tandem-mass spectrometry. Methods The stilbene glucoside was injected into the tail vein of mice. Blood samples were collected from artery in the eyepit. The methanol-protein-precipitated plasma sample was introduced into the liquid chromatography-tandem mass spectrometer directly. The analytical column was C_ 18 column (250 mm×4.6 mm ID, 5 μm). The mobile phase consisted of acetonitrile-methanol-water-formic acid (15∶18∶66∶1) for ES +, acetonitrile-methanol-water (15∶18∶67) for ES -. The UV detection wavelength was set at 320 nm. The mass ion source type was ESI. HV capillary was 3 kV. The dry gas was nitrogen gas and the flow rate was set at 318 L·h -1 . The ion source temperature was 150 ℃. Results The stilbene glucoside and its metabolite were separated completely under the chromatography condition. The ions at m/z 600 and m/z 605 were detected under positive ion polarity while the ions at m/z 581 and m/z 402 were detected under negative ion polarity. Conclusion It was proposed that the metabolite of stilbene glucoside injected in vein was its glucuronide conjugate.
文摘A new andrographolide metabolite 1 was isolated from human urine samples after oral administration. The structure was determined to be 3-carbonylandrographolide-19-O-β-D-glu- curonide on the basis of chemical evidences and spectral analysis, especially by 2D-NMR techni- ques.
文摘目的：通过研究巴西苏木素在大鼠体内的代谢产物,探析其代谢途径。方法：采用UPLC-Q-TOF-MS/MS对灌胃巴西苏木素（30 mg·kg^-1）后大鼠的生物样品（粪便、尿液、血浆、胆汁）进行分析,流动相0.1%甲酸水溶液-乙腈梯度洗脱,流速0.4 m L min^-1,电喷雾离子源,负离子模式,信息依赖性采集模式进行检测,离子喷雾电压-4.5 k V,离子源温度500℃,碰撞电压100 e V,碰撞能量-35 e V,锥孔气流50 L·h^-1,m/z 100-1 100,使用Peak View 1.2软件进行数据处理,提取给药样品的总离子流图谱与空白生物样品对比,通过保留时间、精确相对分子质量及二级质谱的比较与分析,筛选并鉴定其可能的代谢产物。结果：共检测出23个代谢产物,这23个代谢产物是巴西苏木素在大鼠体内甲基化、脱氧、缩氢、羧酸化、水合作用、硫酸化、葡糖糖醛酸化后产生的,其中以硫酸化、葡糖糖醛酸化为主。结论：本实验首次对巴西苏木素在大鼠体内的代谢情况进行研究,能够为该药物的药效学和药理学研究提供一定的物质基础。
