Objective:To investigate the effects of 6 strains of lactobacilli and their main culture metabolites on the morphogenesis of Candida albicans (C. albicans) so as to screen medicinal strains as antifungal agents. Metho...Objective:To investigate the effects of 6 strains of lactobacilli and their main culture metabolites on the morphogenesis of Candida albicans (C. albicans) so as to screen medicinal strains as antifungal agents. Methods: Spent culture supernatant (SCS) at different culture time points, live lactobacilli, heatkilled bacteria and bacterial short-chain fatty acids (SCFA) with different molarities were incubated with C. albicans respectively. Crystal violet-based germ tube assay was used to detect the germination of C. albicans and the inhibitory efficiency of each addition was tested by germination rate. Results: SCS of lactobacillus spp. decreased the germination significantly and live bacteria of L. rhamnosus. GG, L. acidophilus L050103-12 as well as L. johnsonii JCM1022 could partially inhibit the conversion, however, all the heatkilled bacteria failed to control the germ tube formation. In addition, only butyric acid blocked the conversion of yeast to hypha among all the SCFA. Conclusion: The excellent fungistasis activities make L.rhamnosus. GG, L. acidophilus L050103-12 and L. johnsonii JCM1022 potential strains as antifungal drugs and the inhibition seems to have direct correlation to the metabolites.展开更多
The transition of C. albicans from unicellular yeast form to filamentous form i.e., pseudohyphae and hyphae is referred to as morphogenesis. C. albicans has the ability to respond to environmental conditions and accor...The transition of C. albicans from unicellular yeast form to filamentous form i.e., pseudohyphae and hyphae is referred to as morphogenesis. C. albicans has the ability to respond to environmental conditions and accordingly changing its cell morphology. Three main morphological forms of C. albicans are unicellular yeast, pseudohyphae and hyphae. The effect of different growth media (Horse serum medium, RPMI-1640, MSGB), incubation temperatures (34°C, 37°C, 40°C) and pH values (5.4, 6.4, 7.4) on germ tube production by C. albicans was evaluated. Horse serum medium noticeably promotes filamentation while RPMI-1640 medium shows moderate filamentation and MSGB media shows moderately low filamentation. The germ tube of C. albicans developed early in 1.5 hr at high temperature i.e., 40°C. Incubation temperature of 37°C was associated with highest germ tube formation while 34°C shows low filamentation. A pH of 5.4 also induces low filamentation, pH 6.4 gives moderately lower than pH 7.4. A pH of 7.4 was best suited for germ tube induction. The peak of mycelium production appears between 1.5 and 6 hr after inoculation of C. albicans culture. These results suggested that environmental factors are important in selectively favoring yeast or hyphal form, most important being the growth medium, incubation temperature and external pH value.展开更多
目的分析抗白念珠菌芽管胞壁外膜抗原单抗M ab03.2C1-C2靶抗原的性质,探讨该抗原用于实验室检测的可行性。方法制备单克隆抗体,通过SDS-PAGE及W estern B lotting法分析该单抗所识别的抗原的分子量。体外诱导形成不同长度的芽管,分析其...目的分析抗白念珠菌芽管胞壁外膜抗原单抗M ab03.2C1-C2靶抗原的性质,探讨该抗原用于实验室检测的可行性。方法制备单克隆抗体,通过SDS-PAGE及W estern B lotting法分析该单抗所识别的抗原的分子量。体外诱导形成不同长度的芽管,分析其表面靶抗原的形成情况。不同的生化方法处理芽管,采用间接免疫荧光方法,对单抗靶抗原表位性质进行分析。取口腔念珠菌病患者的真菌涂片标本,用IIF方法观察体外诱导培养之芽管与体内白念芽管表面抗原分布异同。结果体外诱导30 m in后白念珠菌芽管表面方可检测到靶抗原。体内白念菌丝与体外诱导菌丝表面靶抗原分布不同。靶抗原表位可能位于N-糖链上。结论M ab03.2C1-C2靶抗原为白念菌丝形成过程中生成的新成分,可能参与白念致病过程。靶抗原为糖蛋白,抗原性显著,对临床应用于系统性白念珠菌感染的检测有意义。展开更多
文摘Objective:To investigate the effects of 6 strains of lactobacilli and their main culture metabolites on the morphogenesis of Candida albicans (C. albicans) so as to screen medicinal strains as antifungal agents. Methods: Spent culture supernatant (SCS) at different culture time points, live lactobacilli, heatkilled bacteria and bacterial short-chain fatty acids (SCFA) with different molarities were incubated with C. albicans respectively. Crystal violet-based germ tube assay was used to detect the germination of C. albicans and the inhibitory efficiency of each addition was tested by germination rate. Results: SCS of lactobacillus spp. decreased the germination significantly and live bacteria of L. rhamnosus. GG, L. acidophilus L050103-12 as well as L. johnsonii JCM1022 could partially inhibit the conversion, however, all the heatkilled bacteria failed to control the germ tube formation. In addition, only butyric acid blocked the conversion of yeast to hypha among all the SCFA. Conclusion: The excellent fungistasis activities make L.rhamnosus. GG, L. acidophilus L050103-12 and L. johnsonii JCM1022 potential strains as antifungal drugs and the inhibition seems to have direct correlation to the metabolites.
文摘The transition of C. albicans from unicellular yeast form to filamentous form i.e., pseudohyphae and hyphae is referred to as morphogenesis. C. albicans has the ability to respond to environmental conditions and accordingly changing its cell morphology. Three main morphological forms of C. albicans are unicellular yeast, pseudohyphae and hyphae. The effect of different growth media (Horse serum medium, RPMI-1640, MSGB), incubation temperatures (34°C, 37°C, 40°C) and pH values (5.4, 6.4, 7.4) on germ tube production by C. albicans was evaluated. Horse serum medium noticeably promotes filamentation while RPMI-1640 medium shows moderate filamentation and MSGB media shows moderately low filamentation. The germ tube of C. albicans developed early in 1.5 hr at high temperature i.e., 40°C. Incubation temperature of 37°C was associated with highest germ tube formation while 34°C shows low filamentation. A pH of 5.4 also induces low filamentation, pH 6.4 gives moderately lower than pH 7.4. A pH of 7.4 was best suited for germ tube induction. The peak of mycelium production appears between 1.5 and 6 hr after inoculation of C. albicans culture. These results suggested that environmental factors are important in selectively favoring yeast or hyphal form, most important being the growth medium, incubation temperature and external pH value.
文摘目的分析抗白念珠菌芽管胞壁外膜抗原单抗M ab03.2C1-C2靶抗原的性质,探讨该抗原用于实验室检测的可行性。方法制备单克隆抗体,通过SDS-PAGE及W estern B lotting法分析该单抗所识别的抗原的分子量。体外诱导形成不同长度的芽管,分析其表面靶抗原的形成情况。不同的生化方法处理芽管,采用间接免疫荧光方法,对单抗靶抗原表位性质进行分析。取口腔念珠菌病患者的真菌涂片标本,用IIF方法观察体外诱导培养之芽管与体内白念芽管表面抗原分布异同。结果体外诱导30 m in后白念珠菌芽管表面方可检测到靶抗原。体内白念菌丝与体外诱导菌丝表面靶抗原分布不同。靶抗原表位可能位于N-糖链上。结论M ab03.2C1-C2靶抗原为白念菌丝形成过程中生成的新成分,可能参与白念致病过程。靶抗原为糖蛋白,抗原性显著,对临床应用于系统性白念珠菌感染的检测有意义。
