Long non-coding RNAs(lncRNAs),which represent a new frontier in molecular biology,play important roles in regulating gene expression at epigenetic,transcriptional and post-transcriptional levels.More and more lncRNAs ...Long non-coding RNAs(lncRNAs),which represent a new frontier in molecular biology,play important roles in regulating gene expression at epigenetic,transcriptional and post-transcriptional levels.More and more lncRNAs have been found to play important roles in normal cell physiological activities,and participate in the development of varieties of tumors and other diseases.Previously,we have only been able to determine the function of lncRNAs through multiple mechanisms,including genetic imprinting,chromatin remodeling,splicing regulation,mRNA decay,and translational regulation.Application of technological advances to research into the function of lncRNAs is extremely important.The major tools for exploring lncRNAs include microarrays,RNA sequencing(RNA-seq),Northern blotting,real-time quantitative reverse transcription-polymerase chain reaction(qRT-PCR),fluorescence in situ hybridization(FISH),RNA interference(RNAi),RNA-binding protein immunoprecipitation(RIP),chromatin isolation by RNA purification(ChIRP),crosslinking-immunopurification(CLIP),and bioinformatic prediction.In this review,we highlight the functions of lncRNAs,and advanced methods to research lncRNA-protein interactions.展开更多
Electroacupuncture has been widely used to treat cognitive impairment after cerebral ischemia,but the underlying mechanism has not yet been fully elucidated.Studies have shown that autophagy plays an important role in...Electroacupuncture has been widely used to treat cognitive impairment after cerebral ischemia,but the underlying mechanism has not yet been fully elucidated.Studies have shown that autophagy plays an important role in the formation and development of cognitive impairment,and the phosphoinositide 3-kinase(PI3K)/Akt signaling pathway plays an important role in autophagy regulation.To investigate the role played by the PI3K/Akt signaling pathway in the electroacupuncture treatment of cerebral ischemia/reperfusion rat models,we first established a rat model of cerebral ischemia/reperfusion through the occlusion of the middle cerebral artery using the suture method.Starting at 2 hours after modeling,electroacupuncture was delivered at the Shenting(GV24)and Baihui(GV20)acupoints,with a dilatational wave(1-20 Hz frequency,2 mA intensity,6 V peak voltage),for 30 minutes/day over 8 consecutive days.Our results showed that electroacupuncture reduced the infarct volume in a rat model of cerebral ischemia/reperfusion injury,increased the mRNA expression levels of the PI3K/Akt signaling pathwayrelated factors Beclin-1,mammalian target of rapamycin(mTOR),and PI3K,increased the protein expression levels of phosphorylated Akt,Beclin-1,PI3K,and mTOR in the ischemic cerebral cortex,and simultaneously reduced p53 mRNA and protein expression levels.In the Morris water maze test,the latency to find the hidden platform was significantly shortened among rats subjected to electroacupuncture stimulation compared with rats without electroacupuncture stimulation.In the spatial probe test,the number of times that a rat crossed the target quadrant was increased in rats subjected to electroacupuncture stimulation compared with rats without electroacupuncture stimulation.Electroacupuncture stimulation applied to the Shenting(GV24)and Baihui(GV20)acupoints activated the PI3K/Akt signaling pathway and improved rat learning and memory impairment.This study was approved by the Animal Ethics Committee of the First Affiliated Hospital of Henan Unive展开更多
对68例冠心病心绞痛患者检测前列腺素、血小板功能和血浆抗凝蛋白 C、蛋白 S 后发现,患者血栓素B_2(TXB_2),β-血小板球蛋白(βTG),血小板第4因子(PF_4)显著增高,6-酮前列环素(6-keto-PGF_(1a))降低。血瘀证与气证(气虚气滞为主)比较,3...对68例冠心病心绞痛患者检测前列腺素、血小板功能和血浆抗凝蛋白 C、蛋白 S 后发现,患者血栓素B_2(TXB_2),β-血小板球蛋白(βTG),血小板第4因子(PF_4)显著增高,6-酮前列环素(6-keto-PGF_(1a))降低。血瘀证与气证(气虚气滞为主)比较,30例血瘀证以 TXB_2增高为主,βTG、PF_4值高,蛋白 C 抗原(PC:Ag)降低,38例气证以6-keto-PGF_(1a)降低为主,βTG,PF_4增高没有血瘀证明显,PC:Ag代偿性增高。这些结果有助于阐明冠心病心绞痛中医辨证分型的内在本质。展开更多
目的探讨康艾注射液联合注射用盐酸吉西他滨治疗非小细胞肺癌的临床疗效。方法选取西安交通大学医学院第一附属医院2013年1月—2015年1月就诊的非小细胞肺癌患者100例,将患者随机分为对照组和治疗组,每组各50例。对照组给予注射用盐酸...目的探讨康艾注射液联合注射用盐酸吉西他滨治疗非小细胞肺癌的临床疗效。方法选取西安交通大学医学院第一附属医院2013年1月—2015年1月就诊的非小细胞肺癌患者100例,将患者随机分为对照组和治疗组,每组各50例。对照组给予注射用盐酸吉西他滨1 200 mg/m^2,加入100 m L生理盐水中,第1、8天静脉滴注。治疗组在对照组基础上静脉滴注康艾注射液50 m L/m^2,加入250 m L生理盐水中,连续使用14 d。两组均连续治疗21 d。观察两组的临床疗效,同时比较治疗前后两组患者的免疫功能指标、蛋白表达和毒副反应。结果治疗后,对照组、治疗组的客观有效率分别为48.0%、66.0%,两组比较差异有统计学意义(P<0.05)。两组疾病控制率比较差异无显著性。治疗后,两组CD^(4+)、CD^(4+)/CD^(8+)明显上升,同组治疗前后比较差异具有统计学意义(P<0.05);且治疗组CD^(3+)、CD^(4+)、CD^(4+)/CD^(8+)明显上升CD^(8+)明显下降,其改善程度优于对照组,两组比较差异具有统计学意义(P<0.05)。治疗后,治疗组Ki67蛋白、bcl-2蛋白表达水平明显降低,bax蛋白表达水平明显升高,两组比较差异有统计学意义(P<0.05)。治疗后,治疗组血小板减少、白细胞下降、红细胞减少发生率明显低于对照组,两组比较差异有统计学意义(P<0.05)。但两组ALT异常升高、恶心呕吐的发生率比较差异无统计学意义。结论康艾注射液联合注射用盐酸吉西他滨治疗非小细胞肺癌具有较好的临床疗效,能够增强患者免疫功能,改善蛋白表达,降低毒副反应,具有一定的临床推广应用价值。展开更多
Enterovirus 71(EV71) is one of the main pathogens that causes hand-foot-and-mouth disease(HFMD). HFMD caused by EV71 infection is mostly self-limited; however, some infections can cause severe neurological diseases, s...Enterovirus 71(EV71) is one of the main pathogens that causes hand-foot-and-mouth disease(HFMD). HFMD caused by EV71 infection is mostly self-limited; however, some infections can cause severe neurological diseases, such as aseptic meningitis, brain stem encephalitis, and even death. There are still no effective clinical drugs used for the prevention and treatment of HFMD. Studying EV71 protein function is essential for elucidating the EV71 replication process and developing anti-EV71 drugs and vaccines. In this review, we summarized the recent progress in the studies of EV71 noncoding regions(50 UTR and 30 UTR) and all structural and nonstructural proteins, especially the key motifs involving in viral infection, replication, and immune regulation. This review will promote our understanding of EV71 virus replication and pathogenesis, and will facilitate the development of novel drugs or vaccines to treat EV71.展开更多
AIM： To examine the significance of branched-chain amino acid （BCAA） treatment before transcatheter arterial chemoembolization （rACE） for hepatocellular carcinoma （HCC）.METHODS： This study included 99 patients...AIM： To examine the significance of branched-chain amino acid （BCAA） treatment before transcatheter arterial chemoembolization （rACE） for hepatocellular carcinoma （HCC）.METHODS： This study included 99 patients who underwent TACE therapy for HCC at our hospital and were followed up without treatment for at least 6 mo between January 2004 and January 2010. They were divided into 2 groups： those receiving BCAA granules （/7 = 40） or regular diet （n = 59, control）. Data obtained were retrospectively analyzed （prior to TACE, and 1 wk, 1, 3, and 6 mo after TACE） in terms of nutritional condition and clinical laboratory parameters （serum albumin level and Child-Pugh score）, both of which are determinants of hepatic functional reserve.RESULTS： The BCAA group comprised 27 males and 13 females with a mean age of 69,9 ：E 8,8 years, The patients of the BCAA group were classified as follows： Child-Pugh A/B/C in 22/15/3 patients, and Stage Ⅱ/Ⅲ/ /IVA HCC in 12/23/5 patients, respectively. The controlgroup comprised 32 males and 27 females with a mean age of 73.2 ± 10.1 years. In the control group, 9 patients had chronic hepatitis, Child-Pugh A/B/C in 39/10/1 patients, and Stage Ⅰ/Ⅱ/Ⅲ/IVA HCC in 1/11/35/12 patients, respectively. Overall, both serum albumin level and Child-Pugh score improved significantly in the BCAA group as compared with the control 3 and 6 mo after TACE （P 〈 0.05）. Further analysis was performed by the following categorization： （1） child-Pugh classification; （2） liver cirrhosis subgroup with a serum albumin level 〉 3.5 g/dL; and （3） epirubicin dose. A similar trend indicating a significant improvement of all variables in the BCAA group was noted （P 〈 0.05）.CONCLUSION： Treatment with BCAA granules in patients who have undergone TACE for HCC is considered useful to maintain their hepatic functional reserve.展开更多
目的:观察研究慢性阻塞性肺疾病急性加重期(acuteex acerbation of chronic obstructive pulmonary disease,A ECOPD)患者肺功能和血清细胞因子水平变化情况,及其与患者吸烟、体重指数(body mass index,BMI)的相关性。方法:选取2020年6...目的:观察研究慢性阻塞性肺疾病急性加重期(acuteex acerbation of chronic obstructive pulmonary disease,A ECOPD)患者肺功能和血清细胞因子水平变化情况,及其与患者吸烟、体重指数(body mass index,BMI)的相关性。方法:选取2020年6月至2020年12月于海南省人民医院海南医学院附属海南医院接受治疗的102例AECOPD患者作为研究对象,将吸烟的50例患者纳入吸烟组,将已戒烟或无吸烟史的52例患者纳入非吸烟组,同时选取50例院内同期进行身体检查的健康志愿者纳入对照组。测定比较3组对象的1秒用力呼气容积(forced expiratory volume in one second,FEV_(1))和一氧化碳弥散量占预计值百分率(percentage of carbon monoxide diffusion to the predicted value,DLco%),同时收集三组对象降钙素原(procalcitonin,PCT)、C反应蛋白(C-reactive protein,CRP)、白细胞介素-6(interleukin-6,IL-6)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)等血清细胞因子表达情况,并分析肺功能及血清细胞因子水平与患者吸烟指数(smoking index,SI)和BMI之间的相关性。结果:吸烟组AECOPD患者的FEV_(1)和DLco%均显著低于非吸烟组和对照组,差异具有统计学意义(P<0.05)。吸烟组AECOPD患者PCT、CRP、IL-6和TNF-α的血清表达水平均显著高于非吸烟组和对照组,差异具有统计学意义(P<0.05)。高SI患者的FEV_(1)和DLco%均显著低于低SI患者,差异具有统计学意义(P<0.05)。高SI患者PCT、CRP、IL-6和TNF-α的血清表达水平均显著高于低S I患者,差异具有统计学意义(P<0.05)。AECOPD患者的SI与PCT、CRP、IL-6和TNF-α呈明显正相关性(P<0.05),与肺功能情况呈明显负相关(P<0.05);A ECOPD患者的BMI与PCT、IL-6和TNF-α呈明显正相关(P<0.05),与肺功能无明显相关(P>0.05)。结论:长期吸烟的AECOPD患者PCT、CRP、IL-6和TNF-α等血清细胞因子的表达水平显著更高,而肺功能状况显著更差。AECOPD患者的吸烟情况和体质量状况均是衡量患者展开更多
基金supported by the National Basic Research Program of China(2010CB912801,2013CB910801)National High Technology Research and Development Program of China(2012AA022501)National Natural Science Foundation of China(31070702,31270836)
文摘Long non-coding RNAs(lncRNAs),which represent a new frontier in molecular biology,play important roles in regulating gene expression at epigenetic,transcriptional and post-transcriptional levels.More and more lncRNAs have been found to play important roles in normal cell physiological activities,and participate in the development of varieties of tumors and other diseases.Previously,we have only been able to determine the function of lncRNAs through multiple mechanisms,including genetic imprinting,chromatin remodeling,splicing regulation,mRNA decay,and translational regulation.Application of technological advances to research into the function of lncRNAs is extremely important.The major tools for exploring lncRNAs include microarrays,RNA sequencing(RNA-seq),Northern blotting,real-time quantitative reverse transcription-polymerase chain reaction(qRT-PCR),fluorescence in situ hybridization(FISH),RNA interference(RNAi),RNA-binding protein immunoprecipitation(RIP),chromatin isolation by RNA purification(ChIRP),crosslinking-immunopurification(CLIP),and bioinformatic prediction.In this review,we highlight the functions of lncRNAs,and advanced methods to research lncRNA-protein interactions.
基金supported by the National Natural Science Foundation of China,No.81574042the Traditional Chinese Medicine Special Research Projects of Henan Province of China,No.2018JDZX011(both to XDF).
文摘Electroacupuncture has been widely used to treat cognitive impairment after cerebral ischemia,but the underlying mechanism has not yet been fully elucidated.Studies have shown that autophagy plays an important role in the formation and development of cognitive impairment,and the phosphoinositide 3-kinase(PI3K)/Akt signaling pathway plays an important role in autophagy regulation.To investigate the role played by the PI3K/Akt signaling pathway in the electroacupuncture treatment of cerebral ischemia/reperfusion rat models,we first established a rat model of cerebral ischemia/reperfusion through the occlusion of the middle cerebral artery using the suture method.Starting at 2 hours after modeling,electroacupuncture was delivered at the Shenting(GV24)and Baihui(GV20)acupoints,with a dilatational wave(1-20 Hz frequency,2 mA intensity,6 V peak voltage),for 30 minutes/day over 8 consecutive days.Our results showed that electroacupuncture reduced the infarct volume in a rat model of cerebral ischemia/reperfusion injury,increased the mRNA expression levels of the PI3K/Akt signaling pathwayrelated factors Beclin-1,mammalian target of rapamycin(mTOR),and PI3K,increased the protein expression levels of phosphorylated Akt,Beclin-1,PI3K,and mTOR in the ischemic cerebral cortex,and simultaneously reduced p53 mRNA and protein expression levels.In the Morris water maze test,the latency to find the hidden platform was significantly shortened among rats subjected to electroacupuncture stimulation compared with rats without electroacupuncture stimulation.In the spatial probe test,the number of times that a rat crossed the target quadrant was increased in rats subjected to electroacupuncture stimulation compared with rats without electroacupuncture stimulation.Electroacupuncture stimulation applied to the Shenting(GV24)and Baihui(GV20)acupoints activated the PI3K/Akt signaling pathway and improved rat learning and memory impairment.This study was approved by the Animal Ethics Committee of the First Affiliated Hospital of Henan Unive
文摘对68例冠心病心绞痛患者检测前列腺素、血小板功能和血浆抗凝蛋白 C、蛋白 S 后发现,患者血栓素B_2(TXB_2),β-血小板球蛋白(βTG),血小板第4因子(PF_4)显著增高,6-酮前列环素(6-keto-PGF_(1a))降低。血瘀证与气证(气虚气滞为主)比较,30例血瘀证以 TXB_2增高为主,βTG、PF_4值高,蛋白 C 抗原(PC:Ag)降低,38例气证以6-keto-PGF_(1a)降低为主,βTG,PF_4增高没有血瘀证明显,PC:Ag代偿性增高。这些结果有助于阐明冠心病心绞痛中医辨证分型的内在本质。
文摘目的探讨康艾注射液联合注射用盐酸吉西他滨治疗非小细胞肺癌的临床疗效。方法选取西安交通大学医学院第一附属医院2013年1月—2015年1月就诊的非小细胞肺癌患者100例,将患者随机分为对照组和治疗组,每组各50例。对照组给予注射用盐酸吉西他滨1 200 mg/m^2,加入100 m L生理盐水中,第1、8天静脉滴注。治疗组在对照组基础上静脉滴注康艾注射液50 m L/m^2,加入250 m L生理盐水中,连续使用14 d。两组均连续治疗21 d。观察两组的临床疗效,同时比较治疗前后两组患者的免疫功能指标、蛋白表达和毒副反应。结果治疗后,对照组、治疗组的客观有效率分别为48.0%、66.0%,两组比较差异有统计学意义(P<0.05)。两组疾病控制率比较差异无显著性。治疗后,两组CD^(4+)、CD^(4+)/CD^(8+)明显上升,同组治疗前后比较差异具有统计学意义(P<0.05);且治疗组CD^(3+)、CD^(4+)、CD^(4+)/CD^(8+)明显上升CD^(8+)明显下降,其改善程度优于对照组,两组比较差异具有统计学意义(P<0.05)。治疗后,治疗组Ki67蛋白、bcl-2蛋白表达水平明显降低,bax蛋白表达水平明显升高,两组比较差异有统计学意义(P<0.05)。治疗后,治疗组血小板减少、白细胞下降、红细胞减少发生率明显低于对照组,两组比较差异有统计学意义(P<0.05)。但两组ALT异常升高、恶心呕吐的发生率比较差异无统计学意义。结论康艾注射液联合注射用盐酸吉西他滨治疗非小细胞肺癌具有较好的临床疗效,能够增强患者免疫功能,改善蛋白表达,降低毒副反应,具有一定的临床推广应用价值。
基金supported by the National Natural Science Foundation of China (Grant 81503118)CAMS Initiative for Innovative Medicine (CAMS-I2 M-1-010)The National Science and Technology Major Project of the Ministry of Science and Technology of China (2018ZX09711003-005-004)
文摘Enterovirus 71(EV71) is one of the main pathogens that causes hand-foot-and-mouth disease(HFMD). HFMD caused by EV71 infection is mostly self-limited; however, some infections can cause severe neurological diseases, such as aseptic meningitis, brain stem encephalitis, and even death. There are still no effective clinical drugs used for the prevention and treatment of HFMD. Studying EV71 protein function is essential for elucidating the EV71 replication process and developing anti-EV71 drugs and vaccines. In this review, we summarized the recent progress in the studies of EV71 noncoding regions(50 UTR and 30 UTR) and all structural and nonstructural proteins, especially the key motifs involving in viral infection, replication, and immune regulation. This review will promote our understanding of EV71 virus replication and pathogenesis, and will facilitate the development of novel drugs or vaccines to treat EV71.
文摘AIM： To examine the significance of branched-chain amino acid （BCAA） treatment before transcatheter arterial chemoembolization （rACE） for hepatocellular carcinoma （HCC）.METHODS： This study included 99 patients who underwent TACE therapy for HCC at our hospital and were followed up without treatment for at least 6 mo between January 2004 and January 2010. They were divided into 2 groups： those receiving BCAA granules （/7 = 40） or regular diet （n = 59, control）. Data obtained were retrospectively analyzed （prior to TACE, and 1 wk, 1, 3, and 6 mo after TACE） in terms of nutritional condition and clinical laboratory parameters （serum albumin level and Child-Pugh score）, both of which are determinants of hepatic functional reserve.RESULTS： The BCAA group comprised 27 males and 13 females with a mean age of 69,9 ：E 8,8 years, The patients of the BCAA group were classified as follows： Child-Pugh A/B/C in 22/15/3 patients, and Stage Ⅱ/Ⅲ/ /IVA HCC in 12/23/5 patients, respectively. The controlgroup comprised 32 males and 27 females with a mean age of 73.2 ± 10.1 years. In the control group, 9 patients had chronic hepatitis, Child-Pugh A/B/C in 39/10/1 patients, and Stage Ⅰ/Ⅱ/Ⅲ/IVA HCC in 1/11/35/12 patients, respectively. Overall, both serum albumin level and Child-Pugh score improved significantly in the BCAA group as compared with the control 3 and 6 mo after TACE （P 〈 0.05）. Further analysis was performed by the following categorization： （1） child-Pugh classification; （2） liver cirrhosis subgroup with a serum albumin level 〉 3.5 g/dL; and （3） epirubicin dose. A similar trend indicating a significant improvement of all variables in the BCAA group was noted （P 〈 0.05）.CONCLUSION： Treatment with BCAA granules in patients who have undergone TACE for HCC is considered useful to maintain their hepatic functional reserve.
文摘目的:观察研究慢性阻塞性肺疾病急性加重期(acuteex acerbation of chronic obstructive pulmonary disease,A ECOPD)患者肺功能和血清细胞因子水平变化情况,及其与患者吸烟、体重指数(body mass index,BMI)的相关性。方法:选取2020年6月至2020年12月于海南省人民医院海南医学院附属海南医院接受治疗的102例AECOPD患者作为研究对象,将吸烟的50例患者纳入吸烟组,将已戒烟或无吸烟史的52例患者纳入非吸烟组,同时选取50例院内同期进行身体检查的健康志愿者纳入对照组。测定比较3组对象的1秒用力呼气容积(forced expiratory volume in one second,FEV_(1))和一氧化碳弥散量占预计值百分率(percentage of carbon monoxide diffusion to the predicted value,DLco%),同时收集三组对象降钙素原(procalcitonin,PCT)、C反应蛋白(C-reactive protein,CRP)、白细胞介素-6(interleukin-6,IL-6)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)等血清细胞因子表达情况,并分析肺功能及血清细胞因子水平与患者吸烟指数(smoking index,SI)和BMI之间的相关性。结果:吸烟组AECOPD患者的FEV_(1)和DLco%均显著低于非吸烟组和对照组,差异具有统计学意义(P<0.05)。吸烟组AECOPD患者PCT、CRP、IL-6和TNF-α的血清表达水平均显著高于非吸烟组和对照组,差异具有统计学意义(P<0.05)。高SI患者的FEV_(1)和DLco%均显著低于低SI患者,差异具有统计学意义(P<0.05)。高SI患者PCT、CRP、IL-6和TNF-α的血清表达水平均显著高于低S I患者,差异具有统计学意义(P<0.05)。AECOPD患者的SI与PCT、CRP、IL-6和TNF-α呈明显正相关性(P<0.05),与肺功能情况呈明显负相关(P<0.05);A ECOPD患者的BMI与PCT、IL-6和TNF-α呈明显正相关(P<0.05),与肺功能无明显相关(P>0.05)。结论:长期吸烟的AECOPD患者PCT、CRP、IL-6和TNF-α等血清细胞因子的表达水平显著更高,而肺功能状况显著更差。AECOPD患者的吸烟情况和体质量状况均是衡量患者
