Objective:Intracerebral delivery of agents in liquid form is usually achieved through commercially available and durable metal needles.However,their size and texture may contribute to mechanical brain damage.Glass pip...Objective:Intracerebral delivery of agents in liquid form is usually achieved through commercially available and durable metal needles.However,their size and texture may contribute to mechanical brain damage.Glass pipettes with a thin tip may significantly reduce injection-associated brain damage but require access to prohibitively expensive programmable pipette pullers.This study is to remove the economic barrier to the application of minimally invasive delivery of therapeutics to the brain,such as chemical compounds,viral vectors,and cells.Methods:We took advantage of the rapid development of free educational online resources and emerging low-cost 3D printers by designing an affordable pipette puller(APP)to remove the cost obstacle.Results:We showed that our APP could produce glass pipettes with a sharp tip opening down to 20μm or less,which is sufficiently thin for the delivery of therapeutics into the brain.A pipeline from pipette pulling to brain injection using low-cost and open-source equipment was established to facilitate the application of the APP.Conclusion:In the spirit of frugal science,our device may democratize glass pipette-puling and substantially promote the application of minimally invasive and precisely controlled delivery of therapeutics to the brain for finding more effective therapies of brain diseases.展开更多
From the author, there are not less than a dozen of rather significant recent publications in scientific editions anticipating some aspects of importance to innovation such as “bigger data”, AI, IP, and frontier tec...From the author, there are not less than a dozen of rather significant recent publications in scientific editions anticipating some aspects of importance to innovation such as “bigger data”, AI, IP, and frontier technology with a central massive contribution in 2020 on AI, IP, and EI. Nonetheless, the IP associated with AI remains still barely covered in scientific publications. Especially patent discussion tends to be rather a legal matter. Another trilogy, 2013, “Business Strategy-IP Strategy-R&D Strategy: An All-in-One Business Model” proposed by the author, marked the advent, and customized implementation of a new strategy level. After the two trilogies’ volumes, the AI-IP “accessibility” chapter was a logical step brought to the attention of a larger public by the author. The time now to bring to light another chapter, namely the IP eligibility of AI innovation steps in ad hoc inventions. The main objectives of this short, principally illustrated communication, are to: 1) Revise the best mode requirement status, i.e. the best way to enable the reproducibility of claimed matter, reviewing its need for improvement when AI is involved. And proposing a unique sequence of evolution inspired by IP’s current and evolving practices. 2) Give a new dimension to visual aids to help the Best Mode description, demystify AI complexity, and underline frontier traits that may hinder a confident adoption or well-argued rejection. 3) Further illustrations take into account the fact that IoT, AI, and 3D can be simpler than perceived. 4) Finally ATA©, Adjacent Technology Analysis, is timely refreshed in a unique challenging, indeed tumultuous, environment. 5) Bias, such as semantic ones is consistently monitored. 6) Overall leaving space for innovative pleasurable interpretation. The emphasis is on educational, illustrative and demonstrative value.展开更多
Based on diffusion of innovation(DOI)theory and economic consumption analysis as well as the concept of frugal innovation,this study develops an extended DOI model that theorizes the characteristics of the diffusion o...Based on diffusion of innovation(DOI)theory and economic consumption analysis as well as the concept of frugal innovation,this study develops an extended DOI model that theorizes the characteristics of the diffusion of frugal information communication technologies(ICTs)and their impacts on the Internet diffusion.This model posits that frugal digital ICTs diffuse more rapidly in developing countries than in developed countries and significantly bridge the digital divide between them.The diffusion of frugal smartphones is investigated as a case of the diffusion of frugal digital ICTs,which provides empirical evidence for the theoretical model presented herein.展开更多
<p align="justify"> <span style="font-family:Verdana;">Soil salinization is one of the major causes of land degradation. In Senegal, this phenomenon continues to grow, making soils unsu...<p align="justify"> <span style="font-family:Verdana;">Soil salinization is one of the major causes of land degradation. In Senegal, this phenomenon continues to grow, making soils unsuitable for agriculture. To rehabilitate salty lands, one of the recommended strategies is the use of salt-tolerant plants. Among them, plants of </span><i><i><span style="font-family:Verdana;">Casuarinaceae</span></i></i><span style="font-family:Verdana;"> family form a relationship with symbiotic microorganisms such as arbuscular mycorrhizal fungi and nitrogen fixing bacteria. It has been shown that symbiotic microorganisms play an important role in the establishment of tolerant plants in saline conditions (Djighaly </span><i><i><span style="font-family:Verdana;">et al</span></i></i><span style="font-family:Verdana;">., 2018). They improve plant performance and reduce transplant shock under salt stress conditions (Diagne </span><i><i><span style="font-family:Verdana;">et al</span></i></i><span style="font-family:Verdana;">., 2014). These microorganisms can be used as biofertilizers. However, inocula containing symbiotic microorganisms are either too expensive or unavailable in many developing countries. The aim of this study is to test alternatively affordable and low-tech solutions to promote symbiotic interactions such as Casuarina crushed nodule, Casuarina rhizosphere soil and Casuarina leaves compost that may contain symbiotic microorganisms and also nutrients such as N and phosphorus. Two species of Casuarina (</span><i><i><span style="font-family:Verdana;">Casuarina equisetifolia</span></i></i><span style="font-family:Verdana;"> L. and </span><i><i><span style="font-family:Verdana;">Casuarina obesa</span></i></i><span style="font-family:Verdana;"> Miq.) were grown in the greenhouse on sterile soil to which an amendment was added (Casuarina crushed nodules, Casuarina Rhizospheric soil or Casuarina leaves compost). Plants were subjected to saline stress. After four months of cultivation, they were harvested and morphological a展开更多
Fluorescent reporters have revolutionized modern applications in the fields of molecular and synthetic biology,enabling applications ranging from education to point-of-care diagnostics.Past advancements in these field...Fluorescent reporters have revolutionized modern applications in the fields of molecular and synthetic biology,enabling applications ranging from education to point-of-care diagnostics.Past advancements in these fields have primarily focused on improving reaction conditions,the development of new applications,and the broad dissemination of these technologies.However,field and classroom-based applications have remained limited in part due to the nature of fluorescent signal detection,which often requires the use of costly lab equipment to observe and quantify fluorescence readouts.Users without access to laboratory equipment rely on qualitative assessments of fluorescence,a process that remains highly variable from user-to-user even within the same classroom.To overcome this challenge,we have developed a foldable illuminator and incubator device to support field-applications of synthetic biology-based biosensors for education and diagnostics.The Fold-Illuminator is an affordable,portable,and recyclable device that allows for the visible detection of fluorescent biomolecules.The Fold-Illuminator’s design allows for assembly in under 10 min,a user can then utilize the optional heating element to incubate biochemical reactions and visualize fluorescence outputs in a defined and light-controlled environment.Interchangeable LED strips and light-filtering screens provide modularity to pair with the fluorescence wavelengths of interest.The user can then unfold the device for convenient storage,transport,or even recycling.The cost for the Fold-Illuminator is$5.58 USD and is compatible with an optional heating element for an additional$3.98 cost,with potential for further reductions in cost for larger quantities.Open-source templates for cutting device parts from paper stock are provided for both printing and cutting by hand;cutting can also be achieved with consumer-grade smart cutting machines such as the Cricut®.Combined with the broad applications of fluorescent reporters,the Fold-Illuminator has the potential to im展开更多
基金UMGCCC American Cancer Society Institutional Research,Grant/Award Numbers:IRG-18-160-16,NIH1R21AG077631-01,R03NS123733Maryland Stem Cell Research Fund,Grant/Award Numbers:2022-MSCRFL-5893,R03NS128459。
文摘Objective:Intracerebral delivery of agents in liquid form is usually achieved through commercially available and durable metal needles.However,their size and texture may contribute to mechanical brain damage.Glass pipettes with a thin tip may significantly reduce injection-associated brain damage but require access to prohibitively expensive programmable pipette pullers.This study is to remove the economic barrier to the application of minimally invasive delivery of therapeutics to the brain,such as chemical compounds,viral vectors,and cells.Methods:We took advantage of the rapid development of free educational online resources and emerging low-cost 3D printers by designing an affordable pipette puller(APP)to remove the cost obstacle.Results:We showed that our APP could produce glass pipettes with a sharp tip opening down to 20μm or less,which is sufficiently thin for the delivery of therapeutics into the brain.A pipeline from pipette pulling to brain injection using low-cost and open-source equipment was established to facilitate the application of the APP.Conclusion:In the spirit of frugal science,our device may democratize glass pipette-puling and substantially promote the application of minimally invasive and precisely controlled delivery of therapeutics to the brain for finding more effective therapies of brain diseases.
文摘From the author, there are not less than a dozen of rather significant recent publications in scientific editions anticipating some aspects of importance to innovation such as “bigger data”, AI, IP, and frontier technology with a central massive contribution in 2020 on AI, IP, and EI. Nonetheless, the IP associated with AI remains still barely covered in scientific publications. Especially patent discussion tends to be rather a legal matter. Another trilogy, 2013, “Business Strategy-IP Strategy-R&D Strategy: An All-in-One Business Model” proposed by the author, marked the advent, and customized implementation of a new strategy level. After the two trilogies’ volumes, the AI-IP “accessibility” chapter was a logical step brought to the attention of a larger public by the author. The time now to bring to light another chapter, namely the IP eligibility of AI innovation steps in ad hoc inventions. The main objectives of this short, principally illustrated communication, are to: 1) Revise the best mode requirement status, i.e. the best way to enable the reproducibility of claimed matter, reviewing its need for improvement when AI is involved. And proposing a unique sequence of evolution inspired by IP’s current and evolving practices. 2) Give a new dimension to visual aids to help the Best Mode description, demystify AI complexity, and underline frontier traits that may hinder a confident adoption or well-argued rejection. 3) Further illustrations take into account the fact that IoT, AI, and 3D can be simpler than perceived. 4) Finally ATA©, Adjacent Technology Analysis, is timely refreshed in a unique challenging, indeed tumultuous, environment. 5) Bias, such as semantic ones is consistently monitored. 6) Overall leaving space for innovative pleasurable interpretation. The emphasis is on educational, illustrative and demonstrative value.
文摘Based on diffusion of innovation(DOI)theory and economic consumption analysis as well as the concept of frugal innovation,this study develops an extended DOI model that theorizes the characteristics of the diffusion of frugal information communication technologies(ICTs)and their impacts on the Internet diffusion.This model posits that frugal digital ICTs diffuse more rapidly in developing countries than in developed countries and significantly bridge the digital divide between them.The diffusion of frugal smartphones is investigated as a case of the diffusion of frugal digital ICTs,which provides empirical evidence for the theoretical model presented herein.
文摘<p align="justify"> <span style="font-family:Verdana;">Soil salinization is one of the major causes of land degradation. In Senegal, this phenomenon continues to grow, making soils unsuitable for agriculture. To rehabilitate salty lands, one of the recommended strategies is the use of salt-tolerant plants. Among them, plants of </span><i><i><span style="font-family:Verdana;">Casuarinaceae</span></i></i><span style="font-family:Verdana;"> family form a relationship with symbiotic microorganisms such as arbuscular mycorrhizal fungi and nitrogen fixing bacteria. It has been shown that symbiotic microorganisms play an important role in the establishment of tolerant plants in saline conditions (Djighaly </span><i><i><span style="font-family:Verdana;">et al</span></i></i><span style="font-family:Verdana;">., 2018). They improve plant performance and reduce transplant shock under salt stress conditions (Diagne </span><i><i><span style="font-family:Verdana;">et al</span></i></i><span style="font-family:Verdana;">., 2014). These microorganisms can be used as biofertilizers. However, inocula containing symbiotic microorganisms are either too expensive or unavailable in many developing countries. The aim of this study is to test alternatively affordable and low-tech solutions to promote symbiotic interactions such as Casuarina crushed nodule, Casuarina rhizosphere soil and Casuarina leaves compost that may contain symbiotic microorganisms and also nutrients such as N and phosphorus. Two species of Casuarina (</span><i><i><span style="font-family:Verdana;">Casuarina equisetifolia</span></i></i><span style="font-family:Verdana;"> L. and </span><i><i><span style="font-family:Verdana;">Casuarina obesa</span></i></i><span style="font-family:Verdana;"> Miq.) were grown in the greenhouse on sterile soil to which an amendment was added (Casuarina crushed nodules, Casuarina Rhizospheric soil or Casuarina leaves compost). Plants were subjected to saline stress. After four months of cultivation, they were harvested and morphological a
文摘Fluorescent reporters have revolutionized modern applications in the fields of molecular and synthetic biology,enabling applications ranging from education to point-of-care diagnostics.Past advancements in these fields have primarily focused on improving reaction conditions,the development of new applications,and the broad dissemination of these technologies.However,field and classroom-based applications have remained limited in part due to the nature of fluorescent signal detection,which often requires the use of costly lab equipment to observe and quantify fluorescence readouts.Users without access to laboratory equipment rely on qualitative assessments of fluorescence,a process that remains highly variable from user-to-user even within the same classroom.To overcome this challenge,we have developed a foldable illuminator and incubator device to support field-applications of synthetic biology-based biosensors for education and diagnostics.The Fold-Illuminator is an affordable,portable,and recyclable device that allows for the visible detection of fluorescent biomolecules.The Fold-Illuminator’s design allows for assembly in under 10 min,a user can then utilize the optional heating element to incubate biochemical reactions and visualize fluorescence outputs in a defined and light-controlled environment.Interchangeable LED strips and light-filtering screens provide modularity to pair with the fluorescence wavelengths of interest.The user can then unfold the device for convenient storage,transport,or even recycling.The cost for the Fold-Illuminator is$5.58 USD and is compatible with an optional heating element for an additional$3.98 cost,with potential for further reductions in cost for larger quantities.Open-source templates for cutting device parts from paper stock are provided for both printing and cutting by hand;cutting can also be achieved with consumer-grade smart cutting machines such as the Cricut®.Combined with the broad applications of fluorescent reporters,the Fold-Illuminator has the potential to im
