目的建立实验室条件下佐剂性关节炎模型的最佳剂量条件.方法取佐剂卡介苗在相应条件下制成Freund's佐剂,按不同剂量给Wista大白鼠进行足跖内皮注射,观察记录24 d.结果及结论0.1 mL Freund's佐剂组在实验室条件下,关节炎模型反...目的建立实验室条件下佐剂性关节炎模型的最佳剂量条件.方法取佐剂卡介苗在相应条件下制成Freund's佐剂,按不同剂量给Wista大白鼠进行足跖内皮注射,观察记录24 d.结果及结论0.1 mL Freund's佐剂组在实验室条件下,关节炎模型反应较典型.展开更多
OBJECTIVE: To evaluate in vitro and in vivo antiarthritic potential of Solanum nigrum (S. nigrum). METHODS: Aqueous methanolic (70∶30) extract of S. nigrum was prepared. The in vitro antiarthritic effect was evaluate...OBJECTIVE: To evaluate in vitro and in vivo antiarthritic potential of Solanum nigrum (S. nigrum). METHODS: Aqueous methanolic (70∶30) extract of S. nigrum was prepared. The in vitro antiarthritic effect was evaluated in terms of its inhibition of protein denaturation and membrane stabilization. While, formaldehyde, complete Freund's adjuvant (CFA) and Collagen induced arthritis rat models were used to study in vivo antiarthritic activities of S. nigrum at dose level of 200, 400 and 800 mg/kg. RESULTS: The extract exhibited inhibition of protein denaturation and protected red blood cell by stabilizing the membranes in a concentration dependent manner, with maximum effect attained at 800 μg/mL. Moreover, there was a marked reduction in paw edema observed in extract treated animals, when compared to arthritic control animals in all in vivo models and 800 mg/kg dose got maximum reduction of paw edema. In CFA and collagen models, plant extract restored body weight, hematologic parameters, radiographic and histopathoOBJECTIVE: To evaluate in vitro and in vivo antiarthritic potential of Solanum nigrum (S. nigrum). METHODS: Aqueous methanolic (70∶30) extract of S. nigrum was prepared. The in vitro antiarthritic effect was evaluated in terms of its inhibition of protein denaturation and membrane stabilization. While, formaldehyde, complete Freund's adjuvant (CFA) and Collagen induced arthritis rat models were used to study in vivo antiarthritic activities of S. nigrum at dose level of 200, 400 and 800 mg/kg. RESULTS: The extract exhibited inhibition of protein denaturation and protected red blood cell by stabilizing the membranes in a concentration dependent manner, with maximum effect attained at 800 μg/mL. Moreover, there was a marked reduction in paw edema observed in extract treated animals, when compared to arthritic control animals in all in vivo models and 800 mg/kg dose got maximum reduction of paw edema. In CFA and collagen models, plant extract restored body weight, hematologic parameters, radiographic an展开更多
OBJECTIVE: To evaluate vitality principle in breast cancer rats by pharmacologically developing a model for anticancer surveillance.METHODS: The breast cancer in rats was replicated with 7,12-Dimethylbenz[a]anthracene...OBJECTIVE: To evaluate vitality principle in breast cancer rats by pharmacologically developing a model for anticancer surveillance.METHODS: The breast cancer in rats was replicated with 7,12-Dimethylbenz[a]anthracene(DMBA, i.g.,100 mg/kg) at d_(001). The anticancer surveillance was defined as the intervals between the primary sensitization and the first challenge stirred with complete Freund's adjuvant(CFA), the various intervals(k = 0.80) were dominated from d_(025)(600.00 h) to d_(095)(2288.82 h). The optimal surveillant status was confirmed with the median effective interval(EI_(50))from tumor volume regressive curve, for developing the pharmacodynamic model. The tumor and tumor infiltrating lymphocyte histopathology was used to confirm the immune surveillance being affected with CFA in breast cancer tumorigenesis.The availability of this model was confirmed with Shugan Liangxue prescription(SLP), from the vitality principle, and assured further from interleukin-12 levels.RESULTS: The regressive curve was set up between the intervals and tumor volumes, the EI_(50) in SLP-treated rats(1475.00 h, Y_(SLP)= 0.1026 +0.8780/[1 + 10^(27.1425-8.565x)]) was postponed, which was 1.87 multiple of the EI_(50) in CFA rats(791.40 h, y =-0.0525 + 0.9452/[1 + 10^(30.4870-10.52x)], so did prepone the curve between the intervals and the immunological biomarker, serum interleukin-12 levels, the EI_(50) in SLP-treated rats(744.90 h, Y_(SLP)=-0.0145 + 0.7455/[1 + 10^(52.09636-18.13x)]) be 0.78 multiple of the EI_(50) in CFA rats(960.10 h, Y_(CFA)= 0.2460 + 0.7270/[1 + 10^(-67.1546 +22.52x)]), this immunological action being mediated the anticancer prognosis. Tumor histology was confirmed the more tumor infiltrating lymphocytes activated in SLP rats with CFA stirred immunity than rats only received CFA.CONCLUSION: The model for anticancer surveillance was pharmacologically established as the optimal interval(791.40 h) between the primary sensitization and the first challenge stirred with complete Freund's adjuvant. This available 展开更多
Rheumatoid arthritis (RA) is a common form of chronic inflammatory arthritis, and it mainly causes the destruction of small joints. The development of this disease is a relatively secret and repeated process, and ther...Rheumatoid arthritis (RA) is a common form of chronic inflammatory arthritis, and it mainly causes the destruction of small joints. The development of this disease is a relatively secret and repeated process, and therefore early diagnosis and evaluation of the disease is usually difficult. In this study, an arthritis model was successfully induced by injecting complete Freund’s adjuvant (CFA) into the toes of lower limbs of Wistar rats. Seven days after injection of CFA, obvious redness and swelling appeared at the toe joints of lower limbs accompanied by more sensitivity to thermal stimulation. Using the ultraweak bio-photon imaging system (UBIS) established by us, the toe joint area of the lower limbs of rats was imaged 7 days after injection of CFA. It was found that the volar part of lower limbs of arthritis rats showed significantly higher biophoton emissions compared with the control group. The results of this study may provide a basis for further research and devel-opment of early diagnosis and assessment of lesion progression of rheumatoid arthritis.展开更多
文摘OBJECTIVE: To evaluate in vitro and in vivo antiarthritic potential of Solanum nigrum (S. nigrum). METHODS: Aqueous methanolic (70∶30) extract of S. nigrum was prepared. The in vitro antiarthritic effect was evaluated in terms of its inhibition of protein denaturation and membrane stabilization. While, formaldehyde, complete Freund's adjuvant (CFA) and Collagen induced arthritis rat models were used to study in vivo antiarthritic activities of S. nigrum at dose level of 200, 400 and 800 mg/kg. RESULTS: The extract exhibited inhibition of protein denaturation and protected red blood cell by stabilizing the membranes in a concentration dependent manner, with maximum effect attained at 800 μg/mL. Moreover, there was a marked reduction in paw edema observed in extract treated animals, when compared to arthritic control animals in all in vivo models and 800 mg/kg dose got maximum reduction of paw edema. In CFA and collagen models, plant extract restored body weight, hematologic parameters, radiographic and histopathoOBJECTIVE: To evaluate in vitro and in vivo antiarthritic potential of Solanum nigrum (S. nigrum). METHODS: Aqueous methanolic (70∶30) extract of S. nigrum was prepared. The in vitro antiarthritic effect was evaluated in terms of its inhibition of protein denaturation and membrane stabilization. While, formaldehyde, complete Freund's adjuvant (CFA) and Collagen induced arthritis rat models were used to study in vivo antiarthritic activities of S. nigrum at dose level of 200, 400 and 800 mg/kg. RESULTS: The extract exhibited inhibition of protein denaturation and protected red blood cell by stabilizing the membranes in a concentration dependent manner, with maximum effect attained at 800 μg/mL. Moreover, there was a marked reduction in paw edema observed in extract treated animals, when compared to arthritic control animals in all in vivo models and 800 mg/kg dose got maximum reduction of paw edema. In CFA and collagen models, plant extract restored body weight, hematologic parameters, radiographic an
基金Supported by the National Natural Science Foundation of China"Via regulating ghrelin axis,Fuzi Lizhong pill effects on chronic atrophic gastritis with Spleen-Yang deficiency","Pharmacological mechanism of Yiqi-huoxue prescription on portal hypertension from inhibiting the oxidase hyperactivity of macrophages in portal triads"(No.81541082,81673674)the Beijing Natural Science Foundation"A pharmcodynamic model of systems biology for the joint actions of molecules from prescriptions with medical plants on portal hypertension"(No.7132150)
文摘OBJECTIVE: To evaluate vitality principle in breast cancer rats by pharmacologically developing a model for anticancer surveillance.METHODS: The breast cancer in rats was replicated with 7,12-Dimethylbenz[a]anthracene(DMBA, i.g.,100 mg/kg) at d_(001). The anticancer surveillance was defined as the intervals between the primary sensitization and the first challenge stirred with complete Freund's adjuvant(CFA), the various intervals(k = 0.80) were dominated from d_(025)(600.00 h) to d_(095)(2288.82 h). The optimal surveillant status was confirmed with the median effective interval(EI_(50))from tumor volume regressive curve, for developing the pharmacodynamic model. The tumor and tumor infiltrating lymphocyte histopathology was used to confirm the immune surveillance being affected with CFA in breast cancer tumorigenesis.The availability of this model was confirmed with Shugan Liangxue prescription(SLP), from the vitality principle, and assured further from interleukin-12 levels.RESULTS: The regressive curve was set up between the intervals and tumor volumes, the EI_(50) in SLP-treated rats(1475.00 h, Y_(SLP)= 0.1026 +0.8780/[1 + 10^(27.1425-8.565x)]) was postponed, which was 1.87 multiple of the EI_(50) in CFA rats(791.40 h, y =-0.0525 + 0.9452/[1 + 10^(30.4870-10.52x)], so did prepone the curve between the intervals and the immunological biomarker, serum interleukin-12 levels, the EI_(50) in SLP-treated rats(744.90 h, Y_(SLP)=-0.0145 + 0.7455/[1 + 10^(52.09636-18.13x)]) be 0.78 multiple of the EI_(50) in CFA rats(960.10 h, Y_(CFA)= 0.2460 + 0.7270/[1 + 10^(-67.1546 +22.52x)]), this immunological action being mediated the anticancer prognosis. Tumor histology was confirmed the more tumor infiltrating lymphocytes activated in SLP rats with CFA stirred immunity than rats only received CFA.CONCLUSION: The model for anticancer surveillance was pharmacologically established as the optimal interval(791.40 h) between the primary sensitization and the first challenge stirred with complete Freund's adjuvant. This available
文摘Rheumatoid arthritis (RA) is a common form of chronic inflammatory arthritis, and it mainly causes the destruction of small joints. The development of this disease is a relatively secret and repeated process, and therefore early diagnosis and evaluation of the disease is usually difficult. In this study, an arthritis model was successfully induced by injecting complete Freund’s adjuvant (CFA) into the toes of lower limbs of Wistar rats. Seven days after injection of CFA, obvious redness and swelling appeared at the toe joints of lower limbs accompanied by more sensitivity to thermal stimulation. Using the ultraweak bio-photon imaging system (UBIS) established by us, the toe joint area of the lower limbs of rats was imaged 7 days after injection of CFA. It was found that the volar part of lower limbs of arthritis rats showed significantly higher biophoton emissions compared with the control group. The results of this study may provide a basis for further research and devel-opment of early diagnosis and assessment of lesion progression of rheumatoid arthritis.
