The diversity of Planctomycetes and related bacteria in 3 types of freshwater wetlands with different anthropogenic disturbances were investigated by cloning and sequencing PCR-amplified partial 16S rRNA genes. Three ...The diversity of Planctomycetes and related bacteria in 3 types of freshwater wetlands with different anthropogenic disturbances were investigated by cloning and sequencing PCR-amplified partial 16S rRNA genes. Three clone libraries were constructed using 16S rRNA-targeted forward PCR primer specific for Planctomycetales and general bacterial reverse primer. Phylogenetic analysis of the 16S rRNA gene sequences defined 95 operational taxonomic units (OTUs) with 163 sequences. The clone libraries covered a wide microbial diversity of Proteobacteria and the Planctomycetes-Verrucomicrobia-Chlamydiales (PVC) superphylum. The majority of the OTUs were related to the phylum of Planctomycetes (33 OTUs), Proteobacteria (22 OTUs) and Verrucomicrobia (22 OTUs). Four known genera from the Planctomycetes phylum were all detected. The genus Pirellula (18 OTUs) dominated the Planctomycetes community, but different patterns of distribution were observed in the wetlands. The littoral wetlands of Baiyangdian Lake with the least anthropogenic disturbances covered more species and showed the highest biodiversity. However, the Jiaxing paddy fields with the highest anthropogenic disturbances showed a higher biodiversity than that in the riparian wetlands of the North Canal. Bacteria distantly related to anammox bacteria were also detected with a small proportion (4 OTUs). It showed that wetlands hold a great biodiversity of phyla Planctomycetes and related bacteria; furthermore, there is ample opportunity to discover novel phylotypes of Planctomycetes in the wetland ecosystems.展开更多
Biomass and carbon stock in a forested areas are now prime important indicators of forest management and climate change mitigation measures. But the accurate estimation of biomass and carbon in trees of forests is now...Biomass and carbon stock in a forested areas are now prime important indicators of forest management and climate change mitigation measures. But the accurate estimation of biomass and carbon in trees of forests is now a challenging issue. In most cases, pantropical and regional biomass models are used frequently to estimate biomass and carbon stock in trees, but these estimation</span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">s</span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> have some uncertainty compared to the species-specific allometric biomass model. </span><i><span style="font-family:Verdana;">Acacia</span></i><span> <i><span style="font-family:Verdana;">nilotica</span></i><span style="font-family:Verdana;">, </span><i><span style="font-family:Verdana;">Casuarina</span></i> <i><span style="font-family:Verdana;">equisetifolia</span></i></span><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Melia</span></i><span style="font-family:Verdana;"> <i>azedarach</i> </span><span style="font-family:Verdana;">have been planted in different areas of Bangladesh considering the species-specific site requirements. While </span><i><span style="font-family:Verdana;">Barringtonia</span></i><span style="font-family:Verdana;"> <i>acutangula</i></span><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Pongamia</span></i><span style="font-family:Verdana;"> <i>pinnata</i> </span><span style="font-family:Verdana;">are the dominant tree species of the freshwater swamp forest of Bangladesh. This study was aimed to develop species-specific allometric biomass models for estimating stem and above ground biomass (TAGB) of these species using the non-destructive method and to compare the efficiency of the derived biomass models with the frequently used regional and pantropical biomass models. Four Ln-based models with diameter at breast height (DBH) and 展开更多
2019年4月至11月在北戴河国家湿地公园进行后生浮游动物和水环境调查,以探究滨海淡水湿地后生浮游动物功能群的季节分布特征及影响因子.采用相似性分析(analysis of similarities,ANOSIM)、非度量多维尺度分析(non-metric multidimensio...2019年4月至11月在北戴河国家湿地公园进行后生浮游动物和水环境调查,以探究滨海淡水湿地后生浮游动物功能群的季节分布特征及影响因子.采用相似性分析(analysis of similarities,ANOSIM)、非度量多维尺度分析(non-metric multidimensional scaling,NMDS)、Mantel检验、冗余分析(redundancy analysis,RDA)及偏最小二乘路径模型(partial least squares-path modeling,Pls-pm)对后生浮游动物功能群和影响因子进行研究,调查期间共采集并鉴定后生浮游动物95种,共划分为8个功能群.结果显示,研究地后生浮游动物功能群组成春季与夏、秋、冬3季差异显著;小型个体组成的功能群在全年密度和生物量中均占优势;非营养盐类环境因子为后生浮游动物功能群季节演替主要驱动因子;营养盐类环境因子对后生浮游动物功能群季节演替影响较小.展开更多
基金supported by the Fundamental Research Funds for the Central Universities(2021ZY90)the National Natural Science Foundation of China(32071525 and 41771547)the National Key Research and Development Program of China(2017YFC0505903).
基金the projects of National Natural Science Foundation of China(21077119)National Basic Research Program of China(2009CB421103)+2 种基金Special Fund of State Key Joint Laboratory of Environment Simulation and Pollution Control(12L03ESPC)Beijing Nova Program(2011095)K.C.Wong Education Foundation,Hong Kong
文摘The diversity of Planctomycetes and related bacteria in 3 types of freshwater wetlands with different anthropogenic disturbances were investigated by cloning and sequencing PCR-amplified partial 16S rRNA genes. Three clone libraries were constructed using 16S rRNA-targeted forward PCR primer specific for Planctomycetales and general bacterial reverse primer. Phylogenetic analysis of the 16S rRNA gene sequences defined 95 operational taxonomic units (OTUs) with 163 sequences. The clone libraries covered a wide microbial diversity of Proteobacteria and the Planctomycetes-Verrucomicrobia-Chlamydiales (PVC) superphylum. The majority of the OTUs were related to the phylum of Planctomycetes (33 OTUs), Proteobacteria (22 OTUs) and Verrucomicrobia (22 OTUs). Four known genera from the Planctomycetes phylum were all detected. The genus Pirellula (18 OTUs) dominated the Planctomycetes community, but different patterns of distribution were observed in the wetlands. The littoral wetlands of Baiyangdian Lake with the least anthropogenic disturbances covered more species and showed the highest biodiversity. However, the Jiaxing paddy fields with the highest anthropogenic disturbances showed a higher biodiversity than that in the riparian wetlands of the North Canal. Bacteria distantly related to anammox bacteria were also detected with a small proportion (4 OTUs). It showed that wetlands hold a great biodiversity of phyla Planctomycetes and related bacteria; furthermore, there is ample opportunity to discover novel phylotypes of Planctomycetes in the wetland ecosystems.
文摘Biomass and carbon stock in a forested areas are now prime important indicators of forest management and climate change mitigation measures. But the accurate estimation of biomass and carbon in trees of forests is now a challenging issue. In most cases, pantropical and regional biomass models are used frequently to estimate biomass and carbon stock in trees, but these estimation</span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">s</span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> have some uncertainty compared to the species-specific allometric biomass model. </span><i><span style="font-family:Verdana;">Acacia</span></i><span> <i><span style="font-family:Verdana;">nilotica</span></i><span style="font-family:Verdana;">, </span><i><span style="font-family:Verdana;">Casuarina</span></i> <i><span style="font-family:Verdana;">equisetifolia</span></i></span><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Melia</span></i><span style="font-family:Verdana;"> <i>azedarach</i> </span><span style="font-family:Verdana;">have been planted in different areas of Bangladesh considering the species-specific site requirements. While </span><i><span style="font-family:Verdana;">Barringtonia</span></i><span style="font-family:Verdana;"> <i>acutangula</i></span><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Pongamia</span></i><span style="font-family:Verdana;"> <i>pinnata</i> </span><span style="font-family:Verdana;">are the dominant tree species of the freshwater swamp forest of Bangladesh. This study was aimed to develop species-specific allometric biomass models for estimating stem and above ground biomass (TAGB) of these species using the non-destructive method and to compare the efficiency of the derived biomass models with the frequently used regional and pantropical biomass models. Four Ln-based models with diameter at breast height (DBH) and
文摘2019年4月至11月在北戴河国家湿地公园进行后生浮游动物和水环境调查,以探究滨海淡水湿地后生浮游动物功能群的季节分布特征及影响因子.采用相似性分析(analysis of similarities,ANOSIM)、非度量多维尺度分析(non-metric multidimensional scaling,NMDS)、Mantel检验、冗余分析(redundancy analysis,RDA)及偏最小二乘路径模型(partial least squares-path modeling,Pls-pm)对后生浮游动物功能群和影响因子进行研究,调查期间共采集并鉴定后生浮游动物95种,共划分为8个功能群.结果显示,研究地后生浮游动物功能群组成春季与夏、秋、冬3季差异显著;小型个体组成的功能群在全年密度和生物量中均占优势;非营养盐类环境因子为后生浮游动物功能群季节演替主要驱动因子;营养盐类环境因子对后生浮游动物功能群季节演替影响较小.
