Objective To study the types of subspecies of Francisella tularensis from China and to investigate the genetic relationships between F. tularensis strains from China and from other countries. Methods Ten strains of F....Objective To study the types of subspecies of Francisella tularensis from China and to investigate the genetic relationships between F. tularensis strains from China and from other countries. Methods Ten strains of F. tulorensis isolated from China were amplified by using typing primers Cl/C4 and RD1. On the basis of the lengths of the polymerase chain reaction （PCR） products, it was concluded that these strains of F. tularensis belonged to the same subspecies. At the same time, thefopA, tul4, and 16S rRNA genes of the I0 strains were amplified, and a three-gene based phylogenetic analysis was performed using the Molecular Evolutionary Genetics Analysis software version 4.0. Results The 10 strains of F. tularensis from China were all identified as belonging to subspecies holarctica （type B）. We found no direct relationship between the genotypes of F. tulurensis subsp. holarctica and the geographical area from where they were isolated. Conclusion The F. tularensis strains isolated from North China mainly belong to subspecies holorctica （type B）. The strains of F. tularensis subsp, holarctico from China may have evolved earlier than those from Europe and North America.展开更多
目的:了解我国北方部分地区宿主动物感染土拉弗菌状况和基因型别。方法:鼠夹诱捕鼠,从鼠脾组织提取DNA,应用巢式PCR检测土拉弗菌,计算并比较各地区及各鼠种阳性率。阳性标本用型特异性引物及短串联重复序列(SSTR9、SSTR16)引物扩增两区...目的:了解我国北方部分地区宿主动物感染土拉弗菌状况和基因型别。方法:鼠夹诱捕鼠,从鼠脾组织提取DNA,应用巢式PCR检测土拉弗菌,计算并比较各地区及各鼠种阳性率。阳性标本用型特异性引物及短串联重复序列(SSTR9、SSTR16)引物扩增两区域并测序,用C lustalX(5.0)和DNA C lub软件对序列进行比较分析。结果:共捕获鼠421只,分属14种鼠种,其中7个鼠种20份标本PCR检测阳性,总阳性率为4.75%。吉林、新疆、黑龙江、内蒙古和浙江的阳性率分别为11.65%,10.00%,6.54%,1.76%和0%。地区间阳性率差异显著(χ2=20.90,P=0.0003);不同鼠种间阳性率未见差异(χ2=11.82,P=0.066)。对20份扩增阳性标本进行型特异性扩增及SSTR9、SSTR16序列测定并对比分析,结果显示均为土拉弗菌B亚型,但型内差别较大,标本及菌株相互比较均显示基因多态性程度高且地区间有交叉。结论:我国北方部分地区可能依然存在B亚型土拉弗菌型感染(宿主动物)情况,且其基因型内差异较大。展开更多
目的检测辽宁省长角血蜱中弗朗西斯菌属及其内共生体的携带情况。方法 2013年7月,在辽宁省采集长角血蜱357只共分成38组,进行土拉弗朗西斯菌(土拉菌)的特异培养和弗朗西斯菌属及其共生体16S r RNA的巢式PCR检测。对有目的片段扩增的PCR...目的检测辽宁省长角血蜱中弗朗西斯菌属及其内共生体的携带情况。方法 2013年7月,在辽宁省采集长角血蜱357只共分成38组,进行土拉弗朗西斯菌(土拉菌)的特异培养和弗朗西斯菌属及其共生体16S r RNA的巢式PCR检测。对有目的片段扩增的PCR产物进行测序和同源性比对,并与网上已公布的弗朗西斯菌属和弗朗西斯菌属(类)内共生体的序列进行系统进化分析。结果有测序结果的13组蜱中,5组为土拉菌,8组为弗朗西斯菌属(类)内共生体。由此推算,河栏镇长角血蜱对于土拉菌的最小携带率为2.12%。系统进化分析显示,5组蜱和土拉菌聚在一个分支上,其余8组和弗朗西斯菌属(类)内共生体聚在一个主干分支上。结论首次发现辽宁省长角血蜱携带土拉菌及弗朗西斯菌属(类)内共生体,对于提醒当地卫生部门做好土拉热防控具有一定意义。展开更多
基金supported by the National Technology Key Special Study Program of the Eleventh Five‐year Plan (No. 2008ZX10004‐010)
文摘Objective To study the types of subspecies of Francisella tularensis from China and to investigate the genetic relationships between F. tularensis strains from China and from other countries. Methods Ten strains of F. tulorensis isolated from China were amplified by using typing primers Cl/C4 and RD1. On the basis of the lengths of the polymerase chain reaction （PCR） products, it was concluded that these strains of F. tularensis belonged to the same subspecies. At the same time, thefopA, tul4, and 16S rRNA genes of the I0 strains were amplified, and a three-gene based phylogenetic analysis was performed using the Molecular Evolutionary Genetics Analysis software version 4.0. Results The 10 strains of F. tularensis from China were all identified as belonging to subspecies holarctica （type B）. We found no direct relationship between the genotypes of F. tulurensis subsp. holarctica and the geographical area from where they were isolated. Conclusion The F. tularensis strains isolated from North China mainly belong to subspecies holorctica （type B）. The strains of F. tularensis subsp, holarctico from China may have evolved earlier than those from Europe and North America.
文摘目的:了解我国北方部分地区宿主动物感染土拉弗菌状况和基因型别。方法:鼠夹诱捕鼠,从鼠脾组织提取DNA,应用巢式PCR检测土拉弗菌,计算并比较各地区及各鼠种阳性率。阳性标本用型特异性引物及短串联重复序列(SSTR9、SSTR16)引物扩增两区域并测序,用C lustalX(5.0)和DNA C lub软件对序列进行比较分析。结果:共捕获鼠421只,分属14种鼠种,其中7个鼠种20份标本PCR检测阳性,总阳性率为4.75%。吉林、新疆、黑龙江、内蒙古和浙江的阳性率分别为11.65%,10.00%,6.54%,1.76%和0%。地区间阳性率差异显著(χ2=20.90,P=0.0003);不同鼠种间阳性率未见差异(χ2=11.82,P=0.066)。对20份扩增阳性标本进行型特异性扩增及SSTR9、SSTR16序列测定并对比分析,结果显示均为土拉弗菌B亚型,但型内差别较大,标本及菌株相互比较均显示基因多态性程度高且地区间有交叉。结论:我国北方部分地区可能依然存在B亚型土拉弗菌型感染(宿主动物)情况,且其基因型内差异较大。
文摘目的检测辽宁省长角血蜱中弗朗西斯菌属及其内共生体的携带情况。方法 2013年7月,在辽宁省采集长角血蜱357只共分成38组,进行土拉弗朗西斯菌(土拉菌)的特异培养和弗朗西斯菌属及其共生体16S r RNA的巢式PCR检测。对有目的片段扩增的PCR产物进行测序和同源性比对,并与网上已公布的弗朗西斯菌属和弗朗西斯菌属(类)内共生体的序列进行系统进化分析。结果有测序结果的13组蜱中,5组为土拉菌,8组为弗朗西斯菌属(类)内共生体。由此推算,河栏镇长角血蜱对于土拉菌的最小携带率为2.12%。系统进化分析显示,5组蜱和土拉菌聚在一个分支上,其余8组和弗朗西斯菌属(类)内共生体聚在一个主干分支上。结论首次发现辽宁省长角血蜱携带土拉菌及弗朗西斯菌属(类)内共生体,对于提醒当地卫生部门做好土拉热防控具有一定意义。
