Pear scab caused by Venturia nashicola is one of the most destructive diseases of pears.Molecular markers linked to scab resistance gene is expected to be useful for improving pear.In this study,the F1 population deri...Pear scab caused by Venturia nashicola is one of the most destructive diseases of pears.Molecular markers linked to scab resistance gene is expected to be useful for improving pear.In this study,the F1 population derived from the cross of ’Huangguan’ and ’Yali’ was analyzed genetically.The resistance of pear to scab was proved to be controlled by a single gene in a dominant manner.Bulked segregant analysis(BSA) was conducted to screen 64 fluorescent AFLP primer pairs.A marker designated as D3-365 was found to be linked to the resistant locus.Selective genotype linkage analysis showed that the genetic distance between the marker and the resistant locus was 14.9 cM.展开更多
Oplegnathusfasciatus （rock bream） is a commercial rocky reef fish species in East Asia that has been considered for aquaculture. We estimated the population genetic diversity and population structure of the species ...Oplegnathusfasciatus （rock bream） is a commercial rocky reef fish species in East Asia that has been considered for aquaculture. We estimated the population genetic diversity and population structure of the species along the coastal waters of China using fluorescent-amplifed fragment length polymorphisms technology. Using 53 individuals from three populations and four pairs of selective primers, we amplified 1 264 bands, 98.73% of which were polymorphic. The Zhoushan population showed the highest Nei＇s genetic diversity and Shannon genetic diversity. The results of analysis of molecular variance （AMOVA） showed that 59.55% of genetic variation existed among populations and 40.45% occurred within populations, which indicated that a significant population genetic structure existed in the species. The pairwise fixation index Fst ranged from 0.20 to 0.63 and were significant after sequential Bonferroni correction. The topology of an unweighted pair group method with arithmetic mean tree showed two significant genealogical branches corresponding to the sampling locations of North and South China. The AMOVA and STRUCTURE analyses suggested that the O.fasciatus populations examined should comprise two stocks.展开更多
文摘Pear scab caused by Venturia nashicola is one of the most destructive diseases of pears.Molecular markers linked to scab resistance gene is expected to be useful for improving pear.In this study,the F1 population derived from the cross of ’Huangguan’ and ’Yali’ was analyzed genetically.The resistance of pear to scab was proved to be controlled by a single gene in a dominant manner.Bulked segregant analysis(BSA) was conducted to screen 64 fluorescent AFLP primer pairs.A marker designated as D3-365 was found to be linked to the resistant locus.Selective genotype linkage analysis showed that the genetic distance between the marker and the resistant locus was 14.9 cM.
基金Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A413-1)the STS(No.KFJ-EW-STS-060-STS)+5 种基金the Natural Science Foundation of Jiangsu Province(No.BK2012222)the Key Laboratory of East China Sea&Oceanic Fishery Resources Exploitation and Utilization,Ministry of Agriculture,China(No.K201204)the Promotive Research Fund for Excellent Young and Middle-Aged Scientists of Shandong Province(No.BS2012HZ025)the National Natural Science Foundation of China(No.41506170)the Technology Foundation for Selected Overseas Chinese ScholarMinistry of Personnel of China(No.2013.01)
文摘Oplegnathusfasciatus （rock bream） is a commercial rocky reef fish species in East Asia that has been considered for aquaculture. We estimated the population genetic diversity and population structure of the species along the coastal waters of China using fluorescent-amplifed fragment length polymorphisms technology. Using 53 individuals from three populations and four pairs of selective primers, we amplified 1 264 bands, 98.73% of which were polymorphic. The Zhoushan population showed the highest Nei＇s genetic diversity and Shannon genetic diversity. The results of analysis of molecular variance （AMOVA） showed that 59.55% of genetic variation existed among populations and 40.45% occurred within populations, which indicated that a significant population genetic structure existed in the species. The pairwise fixation index Fst ranged from 0.20 to 0.63 and were significant after sequential Bonferroni correction. The topology of an unweighted pair group method with arithmetic mean tree showed two significant genealogical branches corresponding to the sampling locations of North and South China. The AMOVA and STRUCTURE analyses suggested that the O.fasciatus populations examined should comprise two stocks.
