Summary： We developed a method that allows us to label nociceptive neurons innervating tooth-pulp in rat trigeminal ganglion neurons using a retrograde fluorescence-tracing method, to re- cord ATP-activated current i...Summary： We developed a method that allows us to label nociceptive neurons innervating tooth-pulp in rat trigeminal ganglion neurons using a retrograde fluorescence-tracing method, to re- cord ATP-activated current in freshly isolated fluorescence-labeled neurons and to conduct single cell immunohistochemical staining for P2X1 and P2X3 subunits in the same neuron. Three types of ATP-activated current in these neurons （F, I and S） were recorded. The cells exhibiting the type F current mainly showed positive staining for P2X3, but negative staining for P2X1. The results provide direct and convincing evidence at the level of single native nociceptive neurons for correlation of the characteristics of ATP-activated currents with their composition of P2Xl and P2X3 subunits and cell size. The results also suggest that the P2X3, but not P2X1, is the main subunit that mediates the fast ATP-activated current in nociceptive neurons.展开更多
Two 3D multifunctional lanthanide metal-organic frameworks（MOFs）, Pr（HTCPS）（H20）·2DMF·C2H2OH·5H2O（JUC-93） and Pr3（TCPS）2（NO3）（H2O）4（DMA）2.2DMA.C2H5OH.3H2O（JUC-94）[H4TCPS=tetrakis（4-ca...Two 3D multifunctional lanthanide metal-organic frameworks（MOFs）, Pr（HTCPS）（H20）·2DMF·C2H2OH·5H2O（JUC-93） and Pr3（TCPS）2（NO3）（H2O）4（DMA）2.2DMA.C2H5OH.3H2O（JUC-94）[H4TCPS=tetrakis（4-carboxyphenyl）- silane, DMF=N,N＇-dimethylformamide, DMA=N,N＇-dimethylacetamide and JUC=Jilin University China] were synthe- sized by the self-assembly of a rigid silicon-centered tetrahedral carboxylate ligand H4TCPS and Pr（III） ions in different solvothermal reactions. X-Ray crystallography revealed that they exhibited a rare CaFE topology framework, constructed from the 4-connected tetrahedral TCPS unit with the 8-connected dinuclear praseodymium cluster unit and trinuclear praseodymium cluster unit, respectively. In addition, the luminescent and magnetic properties of the two compounds were investigated.展开更多
Objective To explore the effects of polysomy 17 on human epidermal growth factor receptor-2(HER-2) testing and study its clinicopathologic significance. Methods We retrospectively analyzed the HER-2 status by fluoresc...Objective To explore the effects of polysomy 17 on human epidermal growth factor receptor-2(HER-2) testing and study its clinicopathologic significance. Methods We retrospectively analyzed the HER-2 status by fluorescence in situ hybridization(FISH) and HER-2展开更多
In recent years the reconstitution of vesicles capable of ATP-driven energy transformation has been carried out in many laboratories[1-5], but the results are not always easily reproducible. It seems to be very import...In recent years the reconstitution of vesicles capable of ATP-driven energy transformation has been carried out in many laboratories[1-5], but the results are not always easily reproducible. It seems to be very important to study the optimal conditions upon which is available the incorporation of the isolated ATPase complex into artificial mem-展开更多
As is well known, the fluorescence spectra of amino-acids can be varied greatly by pH. However, up to date, the effect of organic or inorganic acid on the fluorescence spectra of non-ionic organic compounds is not cle...As is well known, the fluorescence spectra of amino-acids can be varied greatly by pH. However, up to date, the effect of organic or inorganic acid on the fluorescence spectra of non-ionic organic compounds is not clear, although much attention has been paid to this subject in the present decadee. In 1969, Smith reported that the fluorescence of methoxybenzene can be quenched by acid. He has展开更多
This note is to report the exciplex formation between an excited state of the chromophore in poly (polytetramethylene ether glycol terephthalate) (PTMGT,
Since F?rster discovered the fluorescence spectrum of excimer in 1954, a lot of work has been done in this field. It is generally considered that there is no preformed excimer site in the ground state because the abso...Since F?rster discovered the fluorescence spectrum of excimer in 1954, a lot of work has been done in this field. It is generally considered that there is no preformed excimer site in the ground state because the absorption spectrum is unchanged. Excimer is formed only when the molecules have traveled a certain distance by molecular motion and attained a suitable orientation by diffusion-controlled process in the excited state. But Ferguson and Avis et al. discovered the excimer fluorescence展开更多
Objective To investigate the effect and discuss its potential mechanism of sirolimus induced calcium overload and apoptosis in vascular endothelial cells.Meth-ods Human umbilical vein endothelial cells(HUVEC)were expo...Objective To investigate the effect and discuss its potential mechanism of sirolimus induced calcium overload and apoptosis in vascular endothelial cells.Meth-ods Human umbilical vein endothelial cells(HUVEC)were exposed to sirolimus with or without intervention treatment with ryanodine.Effects on cell viability展开更多
基金supported by grants from the National Natural Science Foundation of China (No. 30970927)the Natural Science Foundation of Hubei Province, China (No.2008CDA053)the Wuhan Science and Technology Foundation (Nos. 200970634270, 201250499145-27)
文摘Summary： We developed a method that allows us to label nociceptive neurons innervating tooth-pulp in rat trigeminal ganglion neurons using a retrograde fluorescence-tracing method, to re- cord ATP-activated current in freshly isolated fluorescence-labeled neurons and to conduct single cell immunohistochemical staining for P2X1 and P2X3 subunits in the same neuron. Three types of ATP-activated current in these neurons （F, I and S） were recorded. The cells exhibiting the type F current mainly showed positive staining for P2X3, but negative staining for P2X1. The results provide direct and convincing evidence at the level of single native nociceptive neurons for correlation of the characteristics of ATP-activated currents with their composition of P2Xl and P2X3 subunits and cell size. The results also suggest that the P2X3, but not P2X1, is the main subunit that mediates the fast ATP-activated current in nociceptive neurons.
基金Supported by the National Basic Research Program of China（Nos.2011 CB808703, 2012CB821700） and the National Natural Science Foundation of China（Nos.91022030, 21101072）.
文摘Two 3D multifunctional lanthanide metal-organic frameworks（MOFs）, Pr（HTCPS）（H20）·2DMF·C2H2OH·5H2O（JUC-93） and Pr3（TCPS）2（NO3）（H2O）4（DMA）2.2DMA.C2H5OH.3H2O（JUC-94）[H4TCPS=tetrakis（4-carboxyphenyl）- silane, DMF=N,N＇-dimethylformamide, DMA=N,N＇-dimethylacetamide and JUC=Jilin University China] were synthe- sized by the self-assembly of a rigid silicon-centered tetrahedral carboxylate ligand H4TCPS and Pr（III） ions in different solvothermal reactions. X-Ray crystallography revealed that they exhibited a rare CaFE topology framework, constructed from the 4-connected tetrahedral TCPS unit with the 8-connected dinuclear praseodymium cluster unit and trinuclear praseodymium cluster unit, respectively. In addition, the luminescent and magnetic properties of the two compounds were investigated.
文摘Objective To explore the effects of polysomy 17 on human epidermal growth factor receptor-2(HER-2) testing and study its clinicopathologic significance. Methods We retrospectively analyzed the HER-2 status by fluorescence in situ hybridization(FISH) and HER-2
文摘In recent years the reconstitution of vesicles capable of ATP-driven energy transformation has been carried out in many laboratories[1-5], but the results are not always easily reproducible. It seems to be very important to study the optimal conditions upon which is available the incorporation of the isolated ATPase complex into artificial mem-
文摘As is well known, the fluorescence spectra of amino-acids can be varied greatly by pH. However, up to date, the effect of organic or inorganic acid on the fluorescence spectra of non-ionic organic compounds is not clear, although much attention has been paid to this subject in the present decadee. In 1969, Smith reported that the fluorescence of methoxybenzene can be quenched by acid. He has
文摘This note is to report the exciplex formation between an excited state of the chromophore in poly (polytetramethylene ether glycol terephthalate) (PTMGT,
文摘Since F?rster discovered the fluorescence spectrum of excimer in 1954, a lot of work has been done in this field. It is generally considered that there is no preformed excimer site in the ground state because the absorption spectrum is unchanged. Excimer is formed only when the molecules have traveled a certain distance by molecular motion and attained a suitable orientation by diffusion-controlled process in the excited state. But Ferguson and Avis et al. discovered the excimer fluorescence
文摘Objective To investigate the effect and discuss its potential mechanism of sirolimus induced calcium overload and apoptosis in vascular endothelial cells.Meth-ods Human umbilical vein endothelial cells(HUVEC)were exposed to sirolimus with or without intervention treatment with ryanodine.Effects on cell viability
