Objective To determine the effects of prostaglandin El (PGEl ) on the pulmonary bind flow by using retrograde flush (RF) in lung preservation. Methods Twelve canine donor lungs were treated with RF with UW solution. ...Objective To determine the effects of prostaglandin El (PGEl ) on the pulmonary bind flow by using retrograde flush (RF) in lung preservation. Methods Twelve canine donor lungs were treated with RF with UW solution. In group A (n = 6 ), 250μg of PGE1 wes infused into the pulmonary artery (PA ) before RF and another 250μg wes mixed with UW solution. No PGE1 wes used in group B (n = 6 ). After storage in the mine solution at 4℃ for 22 ~ 25h, the left lung wus transplanted to a recipient followed by 120min reperfusion. Pulmonary bind flow was determined by a microsphere technique. Colored microspheres were infused into the right atrium at the following time: before RF and donor lungs procurement (red ), prior to resecting the recipient left lung (red, served as vaseline), and 120min after donor lung implantation (yellow). Micro- sphere distribution was expressed by optical density per gram lung tissue (OD/g). Results The red microsphere distribution after transplantation in group A wus reduced as compered with baseline (P < 0. 05 ). And more bind flew to the right lung (P < 0. 05 ). The yellow microsphere distribution was also significantly reduced in group A when compered with that of group B (P < 0. 01 ). The hemodynamic bata and bind gases for the two groups were not significantly different. Conclusion After single left lung transplantation, more bind flows to the right side. Pretreatment with PGEl further reduces the flow distribution in the left side.展开更多
Background Myocardial blood flow(MBF) can be quantified with myocardial contrast echocardiography (MCE) during a venous infusion of microbubble. A minimal MBF is required to maintain cell membrane integrity and myocar...Background Myocardial blood flow(MBF) can be quantified with myocardial contrast echocardiography (MCE) during a venous infusion of microbubble. A minimal MBF is required to maintain cell membrane integrity and myocardial viability in ischemic condition. Thus, we hypothesized that MCE could be used to assess myocardial viability by the determination of MBF. Methods and ResultsMCE was performed at 4 hours after ligation of proximal left anterior descending coronary artery in 7 dogs with constant venous infusions of microbubbles. The video intensity versus pulsing interval plots derived from each myocardial pixel were fitted to an exponential function: y=A(1-e-βt), where y is Ⅵ at pulsing interval t, A reflects microvascular cross - sectional area (or myocardial blood volume), and βreflects mean myocardial microbubble velocity. The product of A·β represents MBF. MBF was also obtained by ra-diolabeled microsphere method servered as reference. MBF derived by radiolabeled microsphere - method in the regions of normal, ischemia and infarction was 1.5+0.3, 0.7+0.3, 0. 3+0. 2 mL @ min-1@ g-1 respectively. The product of A·β obtained by MCE in those regions was 52. 46±15. 09, 24. 36±3. 89, 3. 74 ±3. 80 respectively. There was good correlation between normalized MBF and the normalized A·β ( r = 0. 81, P=0. 001). Conclusions MCE has an ability to determine myocardial viability in myocardial infarction canine model.展开更多
文摘Objective To determine the effects of prostaglandin El (PGEl ) on the pulmonary bind flow by using retrograde flush (RF) in lung preservation. Methods Twelve canine donor lungs were treated with RF with UW solution. In group A (n = 6 ), 250μg of PGE1 wes infused into the pulmonary artery (PA ) before RF and another 250μg wes mixed with UW solution. No PGE1 wes used in group B (n = 6 ). After storage in the mine solution at 4℃ for 22 ~ 25h, the left lung wus transplanted to a recipient followed by 120min reperfusion. Pulmonary bind flow was determined by a microsphere technique. Colored microspheres were infused into the right atrium at the following time: before RF and donor lungs procurement (red ), prior to resecting the recipient left lung (red, served as vaseline), and 120min after donor lung implantation (yellow). Micro- sphere distribution was expressed by optical density per gram lung tissue (OD/g). Results The red microsphere distribution after transplantation in group A wus reduced as compered with baseline (P < 0. 05 ). And more bind flew to the right lung (P < 0. 05 ). The yellow microsphere distribution was also significantly reduced in group A when compered with that of group B (P < 0. 01 ). The hemodynamic bata and bind gases for the two groups were not significantly different. Conclusion After single left lung transplantation, more bind flows to the right side. Pretreatment with PGEl further reduces the flow distribution in the left side.
基金This work was supported in part by grants from thescience and technology foundation of China(39870329).
文摘Background Myocardial blood flow(MBF) can be quantified with myocardial contrast echocardiography (MCE) during a venous infusion of microbubble. A minimal MBF is required to maintain cell membrane integrity and myocardial viability in ischemic condition. Thus, we hypothesized that MCE could be used to assess myocardial viability by the determination of MBF. Methods and ResultsMCE was performed at 4 hours after ligation of proximal left anterior descending coronary artery in 7 dogs with constant venous infusions of microbubbles. The video intensity versus pulsing interval plots derived from each myocardial pixel were fitted to an exponential function: y=A(1-e-βt), where y is Ⅵ at pulsing interval t, A reflects microvascular cross - sectional area (or myocardial blood volume), and βreflects mean myocardial microbubble velocity. The product of A·β represents MBF. MBF was also obtained by ra-diolabeled microsphere method servered as reference. MBF derived by radiolabeled microsphere - method in the regions of normal, ischemia and infarction was 1.5+0.3, 0.7+0.3, 0. 3+0. 2 mL @ min-1@ g-1 respectively. The product of A·β obtained by MCE in those regions was 52. 46±15. 09, 24. 36±3. 89, 3. 74 ±3. 80 respectively. There was good correlation between normalized MBF and the normalized A·β ( r = 0. 81, P=0. 001). Conclusions MCE has an ability to determine myocardial viability in myocardial infarction canine model.
