目的研究微生物絮凝剂絮凝菌株S-3的絮凝条件对絮凝活性的影响,确定微生物絮凝剂的最佳絮凝条件.方法改变高岭土悬浊液的pH值、温度条件和生物絮凝剂的加入量来测定高岭土悬浊液的吸光度值.结果絮凝活性物质主要存在于发酵液中,微生物...目的研究微生物絮凝剂絮凝菌株S-3的絮凝条件对絮凝活性的影响,确定微生物絮凝剂的最佳絮凝条件.方法改变高岭土悬浊液的pH值、温度条件和生物絮凝剂的加入量来测定高岭土悬浊液的吸光度值.结果絮凝活性物质主要存在于发酵液中,微生物絮凝剂在pH值为8条件下絮凝活性最高.絮凝剂对温度的变化较敏感,对温度表现出热不稳定性,随着温度的升高絮凝活性降为0,加入6 mL Ca2+离子后絮凝活性提高为92.4%.絮凝活性在一定范围内随着絮凝剂添加量的增加而提高,加入1~2 mL的微生物絮凝剂时絮凝率最高为93.1%.结论由实验室制得的微生物絮凝剂的活性物质主要存在于离心上清液中,高岭土悬浊液絮凝体系的环境对微生物絮凝剂的絮凝效果有影响作用,微生物絮凝剂的主要成分是蛋白质.展开更多
Extracellular polymeric substances(EPS) produced by microorganisms represent biological macromolecules with unfathomable potentials and they are required to be explored further for their potential application as a b...Extracellular polymeric substances(EPS) produced by microorganisms represent biological macromolecules with unfathomable potentials and they are required to be explored further for their potential application as a bioflocculant in various wastewater sludge treatment. Although several studies already exist on biosynthetic pathways of different classical biopolymers like alginate and xanthan, no dedicated studies are available for EPS in sludge. This review highlights the EPS composition, functionality, and biodegradability for its potential use as a carbon source for production of other metabolites. Furthermore, the effect of various extraction methods(physical and chemical) on compositional, structural, physical and functional properties of microbial EPS has been addressed. The vital knowledge of the effect of extraction method on various important attributes of EPS can help to choose the suitable extraction method depending upon the intended use of EPS. The possible use of different molecular biological techniques for enhanced production of desired EPS was summarized.展开更多
文摘目的研究微生物絮凝剂絮凝菌株S-3的絮凝条件对絮凝活性的影响,确定微生物絮凝剂的最佳絮凝条件.方法改变高岭土悬浊液的pH值、温度条件和生物絮凝剂的加入量来测定高岭土悬浊液的吸光度值.结果絮凝活性物质主要存在于发酵液中,微生物絮凝剂在pH值为8条件下絮凝活性最高.絮凝剂对温度的变化较敏感,对温度表现出热不稳定性,随着温度的升高絮凝活性降为0,加入6 mL Ca2+离子后絮凝活性提高为92.4%.絮凝活性在一定范围内随着絮凝剂添加量的增加而提高,加入1~2 mL的微生物絮凝剂时絮凝率最高为93.1%.结论由实验室制得的微生物絮凝剂的活性物质主要存在于离心上清液中,高岭土悬浊液絮凝体系的环境对微生物絮凝剂的絮凝效果有影响作用,微生物絮凝剂的主要成分是蛋白质.
文摘Extracellular polymeric substances(EPS) produced by microorganisms represent biological macromolecules with unfathomable potentials and they are required to be explored further for their potential application as a bioflocculant in various wastewater sludge treatment. Although several studies already exist on biosynthetic pathways of different classical biopolymers like alginate and xanthan, no dedicated studies are available for EPS in sludge. This review highlights the EPS composition, functionality, and biodegradability for its potential use as a carbon source for production of other metabolites. Furthermore, the effect of various extraction methods(physical and chemical) on compositional, structural, physical and functional properties of microbial EPS has been addressed. The vital knowledge of the effect of extraction method on various important attributes of EPS can help to choose the suitable extraction method depending upon the intended use of EPS. The possible use of different molecular biological techniques for enhanced production of desired EPS was summarized.