Three lab-scale vertical-flow constructed wetlands （VFCWs）, including the non-aerated （NA）, intermittently aerated （IA） and continuously aerated （CA） ones, were operated at different hydraulic loading rates （...Three lab-scale vertical-flow constructed wetlands （VFCWs）, including the non-aerated （NA）, intermittently aerated （IA） and continuously aerated （CA） ones, were operated at different hydraulic loading rates （HLRs） to evaluate the effect of artificial aeration on the treatment efficiency of heavily polluted river water. Results indicated that artificial aeration increased the dissolved oxygen （DO） concentrations in IA and CA, which significantly favored the removal of organic matter and NH4＋-N. The DO grads caused by intermittent aeration formed aerobic and anoxic regions in IA and thus promoted the removal of total nitrogen （TN）. Although the removal efficiencies of CODEr, NH4＋-N and TN in the three VFCWs all decreased with an increase in HLR, artificial aeration enhanced the reactor resistance to the fluctuation of pollutant loadings. The maximal removal efficiencies of CODEr, NH4＋-N and total phosphorus （TP） （i.e., 81%, 87% and 37%, respectively） were observed in CA at 19 cm/day HLR, while the maximal TN removal （i.e., 57%） was achieved in IA. Although the improvement of artificial aeration on TP removal was limited, this study has demonstrated the feasibility of applying artificial aeration to VFCWs treating polluted river water, particularly at a high HLR.展开更多
Background Liver targeting drug delivery systems can improve the curative effects and relieve the cytotoxicity of the chemotherapy drugs in the treatment of liver diseases. Nanoparticles carrying therapeutic drugs are...Background Liver targeting drug delivery systems can improve the curative effects and relieve the cytotoxicity of the chemotherapy drugs in the treatment of liver diseases. Nanoparticles carrying therapeutic drugs are currently under hot investigation with great clinical significance. This study was aimed to investigate the different tissue distribution of the adriamycin polybutylcyanoacrylate nanoparticle （ADM-PBCA-NP） in the mice body after an injection via lateral tail vein, and to study the liver targeting effects of ADM-PBCA-NP in different diameters on normal mice liver. Methods One hundred and eighty Kunming mice were randomly divided into 6 groups with 30 mice in each group （5 treatment groups of ADM-PBCA-NP in the different diameter ranges, non-conjugated free adriamycin injection was employed as the control group）. A single dose of either conjugated or free adriamycin equaled 2 mg/kg of body weight was delivered via the tail vein. Five mice in each trail were sacrificed at 5, 15, 30 minutes, 1, 5 and 12 hours postinjection, respectively. The adriamycin cOncentrations in the respectively collected liver, kidney, spleen, heart, lung and plasma were demonstrated using a high performance liquid chromatography with fluorescence detector. Results Compared with the control group, adriamycin was hardly detected in the heart muscle of the treatment groups （P〈0.05）. The nanoparticle-conjugated adriamycin was cleaned up quickly from the kidney tissue. The adriamycin concentrations of the mice liver and spleen in the experimental groups were significantly higher than that in the control group, except for the group with the nanoparticles diameters of （22.3 ±6.2） nm （P〈0.05）. The ADM-PBCA-NP in （101.0±20.3） nm diameter had the highest liver distribution, and the second highest adriamycin distribution in liver was the group of （143.0±23.5） run diameter （P〈0.05）. Moreover, adriamycin was released slowly in the liver during the detection period in the experimental groups. ADM-PBC展开更多
To measure the surface flow in a physical river model, an improved system of Large-Scale Particle Tracking Velocimetry （LSPTV） was proposed and the elements of the PTV system were described. Usually the tracer parti...To measure the surface flow in a physical river model, an improved system of Large-Scale Particle Tracking Velocimetry （LSPTV） was proposed and the elements of the PTV system were described. Usually the tracer particles of a PTV system seeded on water surface tend to form conglomerates due to surface tension of water. In addition, they can not float on water surface when water flow is shallow. Ellipsoid particles were used to avoid the above problems. Another important issue is particle recognition. In order to eliminate the influence of noise, particles were recognized by the processing of multi-frame images. The kernel of the improved PTV system is the algorithm for particle tracking. A new 3-frame PTV algorithm was developed. The performance of this algorithm was compared with the conventional 4-frame PTV algorithm and 2-frame PTV algorithm by means of computer simulation using synthetically generated images. The results show that the new 3-frame PTV algorithm can recover more velocity vectors and have lower relative error. In addition, in order to attain the whole flow field from individual flow fields, the method of stitching individual flow fields by obvious marks was worked out. Then the improved PTV system was applied to the measurement of surface flow field in Model Yellow River and shows good performance.展开更多
Persistent hepatitis B virus （HBV） infection is the most important reason for chronic hepatitis B,hepatic cirrhosis, and hepatocellular carcinoma. T lymphocytes, including CD4^＋ and CD8^＋ T cells, are major compos...Persistent hepatitis B virus （HBV） infection is the most important reason for chronic hepatitis B,hepatic cirrhosis, and hepatocellular carcinoma. T lymphocytes, including CD4^＋ and CD8^＋ T cells, are major composition of host cellular immunity. Furthermore, CD8^＋ cells play a primary role in host immune reaction of anti-tumor and anti-infection. It has been confirmed that HBV infection leads to disorder of cellular immune function in patients, especially disorder in regulative function of T lymphocyte subgroups and cytokine. It has been paid more attention to the expression of HLA class I on hepatocytes infected by HBV and tumor cells, but less to the expression of HLA class I on peripheral blood lymphocytes in the past. In this study, we evaluated the expression of HLA class I and II on peripheral blood lymphocytes in patients with chronic hepatitis B, hepatic cirrhosis, and hepatocellular carcinoma and tried to provide new thought for the mechanism of disorder of celluar immunity.展开更多
All rivers in the Hexi inland region of Gansu Province, China, originate from the northern slope of the Qilian Mountains. They are located in the southern portion of the region and respectively belong to the three lar...All rivers in the Hexi inland region of Gansu Province, China, originate from the northern slope of the Qilian Mountains. They are located in the southern portion of the region and respectively belong to the three large river systems from east to west, the Shiyang, Heihe and Shule river basins. These rivers are supplied by precipitation, snowmelt and ice-melt runoff from the Qilian Mountain area. Therefore, changes of precipitation and temperature in the upstream watersheds of these rivers have an important effect on changes of mountainous runoff and reasonable utilization of water resources in this region. For this reason, the Qilian Mountain area, upstream watersheds and runoff forming areas of these rivers are chosen as the study area. The change characteristics and variation trend of temperature and precipitation in this area under the backdrop of global warming axe analyzed based on observa- tional data of relational weather and hydrologic stations in the area. Results show that temperatures in the upriver mountain areas of these three large river basins have been increasing, although the increasing degree is differentially affected by global warming. The rising extent of annual and seasonal temperatures in the upstream mountain area of the Shule river basin located in the west- em Qilian Mountains, were all largest over the past 50 years. Precipitation in the upstream mountain areas of Hexi region＇ three river basins located respectively in the western, middle and eastern Qilian Mountains have been presenting an increasing trend to varying degrees as a whole for more than 50 years. This means that climate in the upstream mountain areas of Hexi region＇ three river basins are becoming increasingly warmer and moister over the past 50 years, which will be very good for the ecological en- vironment and agricultural production in the region.展开更多
Land use and land cover changes have a great impact on the regional hydrological process. Based on three periods of remote sensing data from the 1960s and the long-term observed data of groundwater from the 1980s, the...Land use and land cover changes have a great impact on the regional hydrological process. Based on three periods of remote sensing data from the 1960s and the long-term observed data of groundwater from the 1980s, the impacts of land use changes on the groundwater system in the middle reach of Heihe River Basin in recent three decades are analyzed by the perspective of groundwater recharge and discharge system. The results indicate that with the different intensities of land use changes, the impacts on the groundwater recharge were 2.602 × 10^8 m^3/a in the former 15 years （1969-1985） and 0.218 × 10^8 m^3/a in the latter 15 years （1986-2000）, and the impacts on the groundwater discharge were 2.035 × 10^8 m^3/a and 4.91 × 10^8 m^3/a respectively. When the groundwater exploitation was in a reasonable range less than 3.0 × 10^8 m^3/a, the land use changes could control the changes of regional groundwater resources. Influenced by the land use changes and the large-scale exploitation in the recent decade, the groundwater resources present apparently regional differences in Zhangye region. Realizing the impact of land use changes on groundwater system and the characteristics of spatial-temporal variations of regional groundwater resources would be very important for reasonably utilizing and managing water and soil resources.展开更多
The concentrations of 16 polycyclic aromatic hydrocarbons（PAHs） were determined in surface sediment samples from nine sites located at the Iguau River Basin in the Metropolitan Region of Curitiba,Brazil to evaluate ...The concentrations of 16 polycyclic aromatic hydrocarbons（PAHs） were determined in surface sediment samples from nine sites located at the Iguau River Basin in the Metropolitan Region of Curitiba,Brazil to evaluate their distribution and sources.The total concentration of the PAHs was greater for sediments from highly urbanized areas,while the sediments from the Iraí Environmental Protection Area（Iraí EPA） showed significantly low concentrations.The sediments from the Iguau and Barigui rivers were classified as highly contaminated,while those from the Cercado and Curralinho rivers were classified as moderately contaminated.The predominance of PAHs containing two to four aromatic rings in most of the samples suggested the direct input of raw sewage into the water resources evaluated.Benzo[g,h,i]perylene,dibenzo[a,h]anthracene and indeno[1,2,3-cd]pyrene were predominant in sediments from the areas under the greatest urban and industrial development.The correlation between thermodynamic stability and the kinetics of evolution presented by the isomeric pairs indicated that combustion is the predominant source of PAHs in the sediments because the combustion of fossil fuels affected most of the points evaluated,followed by combustion of biomass and eventually combustion of oil product inputs.In general,the results showed that areas under strong urban influence,as well as the Iraí EPA,receive contributions of PAHs from similar sources.展开更多
A non-invasive diagnostic approach is crucial for the evaluation of severity of liver disease,treatment decisions,and assessing drug efficacy.This study evaluated plasma proteomic profiling via an N-terminal isotope t...A non-invasive diagnostic approach is crucial for the evaluation of severity of liver disease,treatment decisions,and assessing drug efficacy.This study evaluated plasma proteomic profiling via an N-terminal isotope tagging strategy coupled with liquid chromatography/Fourier transform ion cyclotron resonance mass spectrometry measurement to detect liver fibrosis staging.Pooled plasma from different liver fibrosis stages,which were assessed in advance by the current gold-standard of liver biopsy,was quantitatively analyzed.A total of 72 plasma proteins were found to be dysregulated during the fibrogenesis process,and this finding constituted a valuable candidate plasma biomarker bank for follow-up analysis.Validation results of fibronectin by Western blotting reconfirmed the mass-based data.Ingenuity Pathways Analysis showed four types of metabolic networks for the functional effect of liver fibrosis disease in chronic hepatitis B patients.Consequently,quantitative proteomics via the N-terminal acetyl isotope labeling technique provides an effective and useful tool for screening plasma candidate biomarkers for liver fibrosis.We quantitatively monitored the fibrogenesis process in CHB patients.We discovered many new valuable candidate biomarkers for the diagnosis of liver fibrosis and also partly identified the mechanism involved in liver fibrosis disease.These results provide a clearer understanding of liver fibrosis pathophysiology and will also hopefully lead to improvement of clinical diagnosis and treatment.展开更多
AIM: To analyze changes of the optic nerve head(ONH) and peripapillary region during intraocular pressure(IOP) elevation in patients using spectral domain optical coherence tomography(SD-OCT).METHODS: Both an optic di...AIM: To analyze changes of the optic nerve head(ONH) and peripapillary region during intraocular pressure(IOP) elevation in patients using spectral domain optical coherence tomography(SD-OCT).METHODS: Both an optic disc 200×200 cube scan and a high-definition 5-line raster scan were obtained from open angle glaucoma patients presented with monocular elevation of IOP(≥30 mm Hg) using SD-OCT. Additional baseline characteristics included age, gender, diagnosis,best-corrected visual acuity, refractive error, findings of slit lamp biomicroscopy, findings of dilated stereoscopic examination of the ONH and fundus, IOP, pachymetry findings, and the results of visual field.RESULTS: The 24 patients were selected and divided into two groups: group 1 patients had no history of IOP elevation or glaucoma(n =14), and group 2 patients did have history of IOP elevation or glaucoma(n =10). In each patient, the study eye with elevated IOP was classified into group H(high), and the fellow eye was classified into group L(low). The mean deviation(MD)differed significantly between groups H and L when all eyes were considered(P =0.047) and in group 2(P =0.042), not in group 1(P =0.893). Retinal nerve fiber layer(RNFL) average thickness(P =0.050), rim area(P =0.015),vertical cup/disc ratio(P =0.011), cup volume(P =0.028),inferior quadrant RNFL thickness(P =0.017), and clockhour(1, 5, and 6) RNFL thicknesses(P =0.050, 0.012, and0.018, respectively), cup depth(P =0.008), central prelaminar layer thickness(P =0.023), mid-inferior prelaminar layer thickness(P =0.023), and nasal retinal slope(P =0.034)were significantly different between the eyes with groups H and L.CONCLUSION:RNFLaveragethickness,rim area,vertical cup/disc ratio, cup volume, inferior quadrant RNFL thickness, and clock-hour(1, 5, and 6) RNFL thicknesses significantly changed during acute IOP elevation.展开更多
Background Tissue inhibitor of matrix metaUoproteinase-1 （TIMP-1） is related to the aging of many organs, but few data are available on the change of TIMP-1 in liver aging. The purpose of this study was to investiga...Background Tissue inhibitor of matrix metaUoproteinase-1 （TIMP-1） is related to the aging of many organs, but few data are available on the change of TIMP-1 in liver aging. The purpose of this study was to investigate the expression and role of TIMP-1, matrix metalloproteinase-2 （MMP-2） and MMP-9 in the process of natural aging in the livers of normal and transgenic mice, and to detect the effects of TIMP-1 on oxidative level and anti-oxidative ability of the livers of transgenic young mice. Methods Normal and transgenic mice were divided into 3 groups according to their age： 3-month-old group （n=5）, 12-month-old group （n=5） and 24-month-old group （n=5）. Histopathological changes of the liver were observed after HE and Masson staining. The messenger RNA （mRNA） levels of TIMP-1, MMP-2 and MMP-9 were determined by semi-quantitative reverse transcriptional polymerase chain reaction; protein expression was measured by Western blot in the livers of normal and transgenic mice of various ages. Changes in levels of superoxide dismutase （SOD）, monoamine oxidase （MAO）, malondialdehyde （MDA） as well as oxidative and anti-oxidative ability were measured. Results Histologically, more fatty degeneration and collagen deposition were found in the aging livers of transgenic mice than in those of the normal mice as their age of months increased. The mRNA and protein expressions of TIMP-1 were significantly high in the oldest animals. The histopathological changes, mRNA and protein expressions of TIMP-1 increased significantly in the liver of transgenic mice as compared with normal mice. The expression of MMP-2 and MMP-9 showed a minor change in the process of aging. Liver change and collagen deposition were not observed in young mice, but the activity of SOD decreased （P〈0.05）, and the activity of MAO （P〈0.01） and the content of MDA increased in the liver of transgenic mice （P〈0.01）. Conclusions The expression of TIMP-1 is significantly high in the liver of transgenic mouse i展开更多
The increase of irretrievable river water withdrawals and regulation of river flow has a negative effect on the natural regime of the Aral Sea. The Ainu Darya River and the Syr Darya River Basins are the largest irrig...The increase of irretrievable river water withdrawals and regulation of river flow has a negative effect on the natural regime of the Aral Sea. The Ainu Darya River and the Syr Darya River Basins are the largest irrigated farming areas. Their favorable soil and climatic conditions ensure guaranteed yields of various crops on irrigated lands. Since 1961, for the drastic increase of irretrievable river water withdrawal, mainly for irrigation, the inflow of fiver water into the Aral Sea has started to decrease significantly, accordingly the sea＇s hydrological and hydrochemical regimes disrupted dramatically. The sea level has continued to drop as evaporation exceeds inflow. This negatively transforms the natural environment and worsens socio-economic conditions in Priaralie as a whole, especially in the lower reaches of Amu Darya and Syr Darya, where natural conditions are largely determined by the sea＇s impact. At present, this causes desertification of the nonirrigated zone in the deltas, spreading to new areas as the Aral Sea dries out.展开更多
Purpose: We compared the thickness of circumpapillary retinal nerve fiver layer (cpRNFL) and macular ganglion cell layer with inner plexiform layer (GCL + IPL) using Cirrus HD-OCT (Ver.6.0: Carl Zeiss). Materials and ...Purpose: We compared the thickness of circumpapillary retinal nerve fiver layer (cpRNFL) and macular ganglion cell layer with inner plexiform layer (GCL + IPL) using Cirrus HD-OCT (Ver.6.0: Carl Zeiss). Materials and Methods: This study included 12 eyes of normal controls, 10 eyes of preperimetric glaucoma (PPG) with loss of RNFL either in superior or in inferior hemisphere without visual field defects, and 22 eyes of glaucoma eyes with visual field defects restricted to upper hemifield (UHFD: early 10 eyes, severe 12 eyes). The cpRNFL thickness analyzed from disk center by dividing into 12 sectors. The GCL + IPL thickness analyzed from central fovea by dividing into six sectors. Both compared between normal eye group and other 3 groups using the average value of each sectors. Result: The cpRNFL and the GCL + IPL thickness were obviously thin as compared with normal eyes. Conclusion: Even if it is in the state where abnormalities are not detected using the Humphrey field Analyzer, it is suggested that the early structural change of glaucoma has already arisen.展开更多
基金support from the Ministry of Environmental Protection (No.2008ZX07101-006-08)the Ministry of Science and Technology of China (No. 2009BAC57B02)
文摘Three lab-scale vertical-flow constructed wetlands （VFCWs）, including the non-aerated （NA）, intermittently aerated （IA） and continuously aerated （CA） ones, were operated at different hydraulic loading rates （HLRs） to evaluate the effect of artificial aeration on the treatment efficiency of heavily polluted river water. Results indicated that artificial aeration increased the dissolved oxygen （DO） concentrations in IA and CA, which significantly favored the removal of organic matter and NH4＋-N. The DO grads caused by intermittent aeration formed aerobic and anoxic regions in IA and thus promoted the removal of total nitrogen （TN）. Although the removal efficiencies of CODEr, NH4＋-N and TN in the three VFCWs all decreased with an increase in HLR, artificial aeration enhanced the reactor resistance to the fluctuation of pollutant loadings. The maximal removal efficiencies of CODEr, NH4＋-N and total phosphorus （TP） （i.e., 81%, 87% and 37%, respectively） were observed in CA at 19 cm/day HLR, while the maximal TN removal （i.e., 57%） was achieved in IA. Although the improvement of artificial aeration on TP removal was limited, this study has demonstrated the feasibility of applying artificial aeration to VFCWs treating polluted river water, particularly at a high HLR.
基金This study was supported by a grant from the National 863 Program of China (No. 2002AA216011).
文摘Background Liver targeting drug delivery systems can improve the curative effects and relieve the cytotoxicity of the chemotherapy drugs in the treatment of liver diseases. Nanoparticles carrying therapeutic drugs are currently under hot investigation with great clinical significance. This study was aimed to investigate the different tissue distribution of the adriamycin polybutylcyanoacrylate nanoparticle （ADM-PBCA-NP） in the mice body after an injection via lateral tail vein, and to study the liver targeting effects of ADM-PBCA-NP in different diameters on normal mice liver. Methods One hundred and eighty Kunming mice were randomly divided into 6 groups with 30 mice in each group （5 treatment groups of ADM-PBCA-NP in the different diameter ranges, non-conjugated free adriamycin injection was employed as the control group）. A single dose of either conjugated or free adriamycin equaled 2 mg/kg of body weight was delivered via the tail vein. Five mice in each trail were sacrificed at 5, 15, 30 minutes, 1, 5 and 12 hours postinjection, respectively. The adriamycin cOncentrations in the respectively collected liver, kidney, spleen, heart, lung and plasma were demonstrated using a high performance liquid chromatography with fluorescence detector. Results Compared with the control group, adriamycin was hardly detected in the heart muscle of the treatment groups （P〈0.05）. The nanoparticle-conjugated adriamycin was cleaned up quickly from the kidney tissue. The adriamycin concentrations of the mice liver and spleen in the experimental groups were significantly higher than that in the control group, except for the group with the nanoparticles diameters of （22.3 ±6.2） nm （P〈0.05）. The ADM-PBCA-NP in （101.0±20.3） nm diameter had the highest liver distribution, and the second highest adriamycin distribution in liver was the group of （143.0±23.5） run diameter （P〈0.05）. Moreover, adriamycin was released slowly in the liver during the detection period in the experimental groups. ADM-PBC
基金Project supported by the National Natural Science Foundation of China (Grant Nos. 50479068, 50779014)the Technological Innovation Project of Graduate students in Jiangsu Province (Grant No.1306014)the Project of "Six Talent Peak" of Jiangsu Province (Grant No. 07-A-006).
文摘To measure the surface flow in a physical river model, an improved system of Large-Scale Particle Tracking Velocimetry （LSPTV） was proposed and the elements of the PTV system were described. Usually the tracer particles of a PTV system seeded on water surface tend to form conglomerates due to surface tension of water. In addition, they can not float on water surface when water flow is shallow. Ellipsoid particles were used to avoid the above problems. Another important issue is particle recognition. In order to eliminate the influence of noise, particles were recognized by the processing of multi-frame images. The kernel of the improved PTV system is the algorithm for particle tracking. A new 3-frame PTV algorithm was developed. The performance of this algorithm was compared with the conventional 4-frame PTV algorithm and 2-frame PTV algorithm by means of computer simulation using synthetically generated images. The results show that the new 3-frame PTV algorithm can recover more velocity vectors and have lower relative error. In addition, in order to attain the whole flow field from individual flow fields, the method of stitching individual flow fields by obvious marks was worked out. Then the improved PTV system was applied to the measurement of surface flow field in Model Yellow River and shows good performance.
基金This study was supported by a grant from the Science and Technology Department of Shandong Province (No. 2002BB1CJA8).
文摘Persistent hepatitis B virus （HBV） infection is the most important reason for chronic hepatitis B,hepatic cirrhosis, and hepatocellular carcinoma. T lymphocytes, including CD4^＋ and CD8^＋ T cells, are major composition of host cellular immunity. Furthermore, CD8^＋ cells play a primary role in host immune reaction of anti-tumor and anti-infection. It has been confirmed that HBV infection leads to disorder of cellular immune function in patients, especially disorder in regulative function of T lymphocyte subgroups and cytokine. It has been paid more attention to the expression of HLA class I on hepatocytes infected by HBV and tumor cells, but less to the expression of HLA class I on peripheral blood lymphocytes in the past. In this study, we evaluated the expression of HLA class I and II on peripheral blood lymphocytes in patients with chronic hepatitis B, hepatic cirrhosis, and hepatocellular carcinoma and tried to provide new thought for the mechanism of disorder of celluar immunity.
基金supported by the Open Foundation of Key Laboratory of Ecohydrology in Inland River Basin, Chinese Academy of Sciences and National Nature Science Foundation of China (No. 9112502)The Coupling and Modeling of Eco-hydrological Processes in the Upper Reaches of Heihe River (No. 91225302)+1 种基金Research of Eco-hydrological Response Units in Heihe River Basin Based on the Method of Concept Lattice (No. 41240002)System Behaviors and Regulation of Ecohydrological Processes in the Middle and Lower Heihe River Basin (91225301)
文摘All rivers in the Hexi inland region of Gansu Province, China, originate from the northern slope of the Qilian Mountains. They are located in the southern portion of the region and respectively belong to the three large river systems from east to west, the Shiyang, Heihe and Shule river basins. These rivers are supplied by precipitation, snowmelt and ice-melt runoff from the Qilian Mountain area. Therefore, changes of precipitation and temperature in the upstream watersheds of these rivers have an important effect on changes of mountainous runoff and reasonable utilization of water resources in this region. For this reason, the Qilian Mountain area, upstream watersheds and runoff forming areas of these rivers are chosen as the study area. The change characteristics and variation trend of temperature and precipitation in this area under the backdrop of global warming axe analyzed based on observa- tional data of relational weather and hydrologic stations in the area. Results show that temperatures in the upriver mountain areas of these three large river basins have been increasing, although the increasing degree is differentially affected by global warming. The rising extent of annual and seasonal temperatures in the upstream mountain area of the Shule river basin located in the west- em Qilian Mountains, were all largest over the past 50 years. Precipitation in the upstream mountain areas of Hexi region＇ three river basins located respectively in the western, middle and eastern Qilian Mountains have been presenting an increasing trend to varying degrees as a whole for more than 50 years. This means that climate in the upstream mountain areas of Hexi region＇ three river basins are becoming increasingly warmer and moister over the past 50 years, which will be very good for the ecological en- vironment and agricultural production in the region.
基金NationalNaturalScience Foundation of China,No.40171002China-Japan Cooperation Project "Estim ation ofoasisadaptability to waterresourceunderchanging environment"
文摘Land use and land cover changes have a great impact on the regional hydrological process. Based on three periods of remote sensing data from the 1960s and the long-term observed data of groundwater from the 1980s, the impacts of land use changes on the groundwater system in the middle reach of Heihe River Basin in recent three decades are analyzed by the perspective of groundwater recharge and discharge system. The results indicate that with the different intensities of land use changes, the impacts on the groundwater recharge were 2.602 × 10^8 m^3/a in the former 15 years （1969-1985） and 0.218 × 10^8 m^3/a in the latter 15 years （1986-2000）, and the impacts on the groundwater discharge were 2.035 × 10^8 m^3/a and 4.91 × 10^8 m^3/a respectively. When the groundwater exploitation was in a reasonable range less than 3.0 × 10^8 m^3/a, the land use changes could control the changes of regional groundwater resources. Influenced by the land use changes and the large-scale exploitation in the recent decade, the groundwater resources present apparently regional differences in Zhangye region. Realizing the impact of land use changes on groundwater system and the characteristics of spatial-temporal variations of regional groundwater resources would be very important for reasonably utilizing and managing water and soil resources.
文摘The concentrations of 16 polycyclic aromatic hydrocarbons（PAHs） were determined in surface sediment samples from nine sites located at the Iguau River Basin in the Metropolitan Region of Curitiba,Brazil to evaluate their distribution and sources.The total concentration of the PAHs was greater for sediments from highly urbanized areas,while the sediments from the Iraí Environmental Protection Area（Iraí EPA） showed significantly low concentrations.The sediments from the Iguau and Barigui rivers were classified as highly contaminated,while those from the Cercado and Curralinho rivers were classified as moderately contaminated.The predominance of PAHs containing two to four aromatic rings in most of the samples suggested the direct input of raw sewage into the water resources evaluated.Benzo[g,h,i]perylene,dibenzo[a,h]anthracene and indeno[1,2,3-cd]pyrene were predominant in sediments from the areas under the greatest urban and industrial development.The correlation between thermodynamic stability and the kinetics of evolution presented by the isomeric pairs indicated that combustion is the predominant source of PAHs in the sediments because the combustion of fossil fuels affected most of the points evaluated,followed by combustion of biomass and eventually combustion of oil product inputs.In general,the results showed that areas under strong urban influence,as well as the Iraí EPA,receive contributions of PAHs from similar sources.
基金supported by the National Basic Research Program of China(Grant Nos.2011CB910601,2011CB910700,2010CB912700 and 2011CB505304)the National High Technology Research and Development Program of China (Grant No.2006AA02A308)+4 种基金National Natural Science Foundation of China(Grant Nos.30700356,30700988,30972909,81000192,81001470 and 81010064)Chinese State Key Project Specialized for Infectious Diseases (Grant Nos.2008ZX10002-016 and 2009ZX10004-103)the National Key Technologies R&D Program for New Drugs (Grant No.2009ZX09301-002)the International Scientific Collaboration Program (Grant Nos.2009DFB33070 and 2010DFA31260)State Key Laboratory of Proteomics(Grant Nos.SKLP-Y200901 and SKLP-O200901)
文摘A non-invasive diagnostic approach is crucial for the evaluation of severity of liver disease,treatment decisions,and assessing drug efficacy.This study evaluated plasma proteomic profiling via an N-terminal isotope tagging strategy coupled with liquid chromatography/Fourier transform ion cyclotron resonance mass spectrometry measurement to detect liver fibrosis staging.Pooled plasma from different liver fibrosis stages,which were assessed in advance by the current gold-standard of liver biopsy,was quantitatively analyzed.A total of 72 plasma proteins were found to be dysregulated during the fibrogenesis process,and this finding constituted a valuable candidate plasma biomarker bank for follow-up analysis.Validation results of fibronectin by Western blotting reconfirmed the mass-based data.Ingenuity Pathways Analysis showed four types of metabolic networks for the functional effect of liver fibrosis disease in chronic hepatitis B patients.Consequently,quantitative proteomics via the N-terminal acetyl isotope labeling technique provides an effective and useful tool for screening plasma candidate biomarkers for liver fibrosis.We quantitatively monitored the fibrogenesis process in CHB patients.We discovered many new valuable candidate biomarkers for the diagnosis of liver fibrosis and also partly identified the mechanism involved in liver fibrosis disease.These results provide a clearer understanding of liver fibrosis pathophysiology and will also hopefully lead to improvement of clinical diagnosis and treatment.
文摘AIM: To analyze changes of the optic nerve head(ONH) and peripapillary region during intraocular pressure(IOP) elevation in patients using spectral domain optical coherence tomography(SD-OCT).METHODS: Both an optic disc 200×200 cube scan and a high-definition 5-line raster scan were obtained from open angle glaucoma patients presented with monocular elevation of IOP(≥30 mm Hg) using SD-OCT. Additional baseline characteristics included age, gender, diagnosis,best-corrected visual acuity, refractive error, findings of slit lamp biomicroscopy, findings of dilated stereoscopic examination of the ONH and fundus, IOP, pachymetry findings, and the results of visual field.RESULTS: The 24 patients were selected and divided into two groups: group 1 patients had no history of IOP elevation or glaucoma(n =14), and group 2 patients did have history of IOP elevation or glaucoma(n =10). In each patient, the study eye with elevated IOP was classified into group H(high), and the fellow eye was classified into group L(low). The mean deviation(MD)differed significantly between groups H and L when all eyes were considered(P =0.047) and in group 2(P =0.042), not in group 1(P =0.893). Retinal nerve fiber layer(RNFL) average thickness(P =0.050), rim area(P =0.015),vertical cup/disc ratio(P =0.011), cup volume(P =0.028),inferior quadrant RNFL thickness(P =0.017), and clockhour(1, 5, and 6) RNFL thicknesses(P =0.050, 0.012, and0.018, respectively), cup depth(P =0.008), central prelaminar layer thickness(P =0.023), mid-inferior prelaminar layer thickness(P =0.023), and nasal retinal slope(P =0.034)were significantly different between the eyes with groups H and L.CONCLUSION:RNFLaveragethickness,rim area,vertical cup/disc ratio, cup volume, inferior quadrant RNFL thickness, and clock-hour(1, 5, and 6) RNFL thicknesses significantly changed during acute IOP elevation.
基金This study was supported by grants from the Major State Basic Research Development Program of China (973 Program, Creative Research Group Fund of the National Science Foundation of China (No. 30121005)Postdoctor Foundation of China (No. 2004035048).
文摘Background Tissue inhibitor of matrix metaUoproteinase-1 （TIMP-1） is related to the aging of many organs, but few data are available on the change of TIMP-1 in liver aging. The purpose of this study was to investigate the expression and role of TIMP-1, matrix metalloproteinase-2 （MMP-2） and MMP-9 in the process of natural aging in the livers of normal and transgenic mice, and to detect the effects of TIMP-1 on oxidative level and anti-oxidative ability of the livers of transgenic young mice. Methods Normal and transgenic mice were divided into 3 groups according to their age： 3-month-old group （n=5）, 12-month-old group （n=5） and 24-month-old group （n=5）. Histopathological changes of the liver were observed after HE and Masson staining. The messenger RNA （mRNA） levels of TIMP-1, MMP-2 and MMP-9 were determined by semi-quantitative reverse transcriptional polymerase chain reaction; protein expression was measured by Western blot in the livers of normal and transgenic mice of various ages. Changes in levels of superoxide dismutase （SOD）, monoamine oxidase （MAO）, malondialdehyde （MDA） as well as oxidative and anti-oxidative ability were measured. Results Histologically, more fatty degeneration and collagen deposition were found in the aging livers of transgenic mice than in those of the normal mice as their age of months increased. The mRNA and protein expressions of TIMP-1 were significantly high in the oldest animals. The histopathological changes, mRNA and protein expressions of TIMP-1 increased significantly in the liver of transgenic mice as compared with normal mice. The expression of MMP-2 and MMP-9 showed a minor change in the process of aging. Liver change and collagen deposition were not observed in young mice, but the activity of SOD decreased （P〈0.05）, and the activity of MAO （P〈0.01） and the content of MDA increased in the liver of transgenic mice （P〈0.01）. Conclusions The expression of TIMP-1 is significantly high in the liver of transgenic mouse i
文摘The increase of irretrievable river water withdrawals and regulation of river flow has a negative effect on the natural regime of the Aral Sea. The Ainu Darya River and the Syr Darya River Basins are the largest irrigated farming areas. Their favorable soil and climatic conditions ensure guaranteed yields of various crops on irrigated lands. Since 1961, for the drastic increase of irretrievable river water withdrawal, mainly for irrigation, the inflow of fiver water into the Aral Sea has started to decrease significantly, accordingly the sea＇s hydrological and hydrochemical regimes disrupted dramatically. The sea level has continued to drop as evaporation exceeds inflow. This negatively transforms the natural environment and worsens socio-economic conditions in Priaralie as a whole, especially in the lower reaches of Amu Darya and Syr Darya, where natural conditions are largely determined by the sea＇s impact. At present, this causes desertification of the nonirrigated zone in the deltas, spreading to new areas as the Aral Sea dries out.
文摘Purpose: We compared the thickness of circumpapillary retinal nerve fiver layer (cpRNFL) and macular ganglion cell layer with inner plexiform layer (GCL + IPL) using Cirrus HD-OCT (Ver.6.0: Carl Zeiss). Materials and Methods: This study included 12 eyes of normal controls, 10 eyes of preperimetric glaucoma (PPG) with loss of RNFL either in superior or in inferior hemisphere without visual field defects, and 22 eyes of glaucoma eyes with visual field defects restricted to upper hemifield (UHFD: early 10 eyes, severe 12 eyes). The cpRNFL thickness analyzed from disk center by dividing into 12 sectors. The GCL + IPL thickness analyzed from central fovea by dividing into six sectors. Both compared between normal eye group and other 3 groups using the average value of each sectors. Result: The cpRNFL and the GCL + IPL thickness were obviously thin as compared with normal eyes. Conclusion: Even if it is in the state where abnormalities are not detected using the Humphrey field Analyzer, it is suggested that the early structural change of glaucoma has already arisen.
