[ Objective] To isolate fetal fibroblasts from Tibetan yellow cattle and thus to provide the ideal biological material for cloning and cellular biology of animals inhabiting in the Tibet Plateau. [ Method] The fibrobl...[ Objective] To isolate fetal fibroblasts from Tibetan yellow cattle and thus to provide the ideal biological material for cloning and cellular biology of animals inhabiting in the Tibet Plateau. [ Method] The fibroblast cells were isolated from tissues of Tibetan yellow fetus by explant culture method. The subculture, morphology and dynamics were observed. [ Result] The fetal fibroblasts of Tibetan yellow cattle had typical morphology of fibroblast calls. The isolated cells had three growth stages including retention period （0 -2 d）, logarithmic phase （2 -7 d） and platform phase （7 -8 d）. Its doubling time was 60 h. [ Conclusion] Fetal fibroblasts of Tibetan yellow cattle have been successfully cultured.展开更多
Homeobox A10(Hoxa10) gene is one of the most important candidate genes associated with the reproductive performance of humans and mice. Overexpression of Hoxa10 in mouse endometrium can increase litter size. Moreover,...Homeobox A10(Hoxa10) gene is one of the most important candidate genes associated with the reproductive performance of humans and mice. Overexpression of Hoxa10 in mouse endometrium can increase litter size. Moreover, Hoxa10 plays a key role in regulating the embryo implantation of sows. This study aimed to generate transgenic pigs using Hoxa10 via somatic cell nuclear transfer(SCNT). We established seven Hoxa10-transgenic cell lines, and two of the cell lines were selected as nuclear donors for the transfer. A total of 1 270 cloned embryos were generated and transferred to five surrogate mothers(Landrace×Yorkshire). Eight cloned male piglets were produced including one with cryptorchidism. Six transgenic piglets grew up healthy and produced 56 offspring. Finally, we obtained six transgenic male pigs and 26 transgenic positive offspring that can be used to further study the regulatory mechanism of Hoxa10 on the reproductive performance of pigs.展开更多
基金supported by National Natural Science Foundation of China (31040077)University Teachers Team Construction Program of"211 Project"(SZRC-211-01)Regional Natural Science Foundation of Tibet Science and Technology Department (2010-17-02)
文摘[ Objective] To isolate fetal fibroblasts from Tibetan yellow cattle and thus to provide the ideal biological material for cloning and cellular biology of animals inhabiting in the Tibet Plateau. [ Method] The fibroblast cells were isolated from tissues of Tibetan yellow fetus by explant culture method. The subculture, morphology and dynamics were observed. [ Result] The fetal fibroblasts of Tibetan yellow cattle had typical morphology of fibroblast calls. The isolated cells had three growth stages including retention period （0 -2 d）, logarithmic phase （2 -7 d） and platform phase （7 -8 d）. Its doubling time was 60 h. [ Conclusion] Fetal fibroblasts of Tibetan yellow cattle have been successfully cultured.
基金supported by the National Major Special Project on New Varieties Cultivation for Transgenic Organisms,China(2014ZX08006-005 and 2014ZX0800950B)
文摘Homeobox A10(Hoxa10) gene is one of the most important candidate genes associated with the reproductive performance of humans and mice. Overexpression of Hoxa10 in mouse endometrium can increase litter size. Moreover, Hoxa10 plays a key role in regulating the embryo implantation of sows. This study aimed to generate transgenic pigs using Hoxa10 via somatic cell nuclear transfer(SCNT). We established seven Hoxa10-transgenic cell lines, and two of the cell lines were selected as nuclear donors for the transfer. A total of 1 270 cloned embryos were generated and transferred to five surrogate mothers(Landrace×Yorkshire). Eight cloned male piglets were produced including one with cryptorchidism. Six transgenic piglets grew up healthy and produced 56 offspring. Finally, we obtained six transgenic male pigs and 26 transgenic positive offspring that can be used to further study the regulatory mechanism of Hoxa10 on the reproductive performance of pigs.
