AIM： To determine if brain-derived neurotrophic factor （BDNF） could offer protention to retinal ganglion cells following a superior colliculus （SC） lesion in mice using pattern electroretinogram （PERG） and opti...AIM： To determine if brain-derived neurotrophic factor （BDNF） could offer protention to retinal ganglion cells following a superior colliculus （SC） lesion in mice using pattern electroretinogram （PERG） and optical coherence tomography （OCT） as a measures of ganglion cell response and retinal health. METHODS： Seven C57BIJ6J mice with BDNF protection were tested with PERG and OCT before and after SC lesions, RESULTS： Compared with baseline PERG, the amplitude of PERG decreased 11.7% after SC lesions, but not significantly（P〉0.05）. Through fast Fourier transform （FFT） analysis of the PERGs before and after SC lesions, it was found that dominant frequency of PERGs stayed unchanged, suggesting that the ganglion cells of the retina remained relatively healthy inspite of damage to the ends of the ganglion cell axons. Also, OCT showed no changes in retinal thickness after lesions. CONCLUSION： It was concluded that BDNF is essential component of normal retinal and helps retina keeping normal function. While retina lack of BDNF, ex vivo resource of BDNF provides protection to the sick retina. It implies that BDNF is a kind therapeutic neurotrophic factor to retina neurodegeneration diseases, such as glaucoma, age related macular degeneration.展开更多
文摘AIM： To determine if brain-derived neurotrophic factor （BDNF） could offer protention to retinal ganglion cells following a superior colliculus （SC） lesion in mice using pattern electroretinogram （PERG） and optical coherence tomography （OCT） as a measures of ganglion cell response and retinal health. METHODS： Seven C57BIJ6J mice with BDNF protection were tested with PERG and OCT before and after SC lesions, RESULTS： Compared with baseline PERG, the amplitude of PERG decreased 11.7% after SC lesions, but not significantly（P〉0.05）. Through fast Fourier transform （FFT） analysis of the PERGs before and after SC lesions, it was found that dominant frequency of PERGs stayed unchanged, suggesting that the ganglion cells of the retina remained relatively healthy inspite of damage to the ends of the ganglion cell axons. Also, OCT showed no changes in retinal thickness after lesions. CONCLUSION： It was concluded that BDNF is essential component of normal retinal and helps retina keeping normal function. While retina lack of BDNF, ex vivo resource of BDNF provides protection to the sick retina. It implies that BDNF is a kind therapeutic neurotrophic factor to retina neurodegeneration diseases, such as glaucoma, age related macular degeneration.
基金The project was supported by National Natural Science Foundation of China(11231005)Doctoral Program Foundation of the Ministry of Education of China(20110076110004)
