Faster and better wound healing is a critical medical issue.Because the repair process of wounds is closely related to revascularization,accurate early assessment and postoperative monitoring are very important for es...Faster and better wound healing is a critical medical issue.Because the repair process of wounds is closely related to revascularization,accurate early assessment and postoperative monitoring are very important for establishing an optimal treatment plan.Herein,we present an extended depth-of-field photoacoustic microscopy system(E-DOF-PAM)that can achieve a constant spatial resolution and relatively uniform excitation efficiency over a long axial range.The superior performance of the system was verified by phantom and in vivo experiments.Furthermore,the system was applied to the imaging of normal and trauma sites of volunteers,and the experimental results accurately revealed the morphological differences between the normal and traumatized skin of the epidermis and dermis.These results demonstrated that the E-DOF-PAM is a powerful tool for observing and understanding the pathophysiology of cutaneous wound healing.展开更多
Microfuidic systems have been widely utilized in high-throughput biology analysis,but thedificulties in iquid manipulation and cell cultivation limit its application.This work has developed a new digital microfluidic(...Microfuidic systems have been widely utilized in high-throughput biology analysis,but thedificulties in iquid manipulation and cell cultivation limit its application.This work has developed a new digital microfluidic(DMF)system for on-demand droplet control.By adopting anextending-depth-of-field(EDoF)phase modulator to the optical system,the entire depth of themicrofluidic channel can be covered in one image without any refocusing process,ensuring that 95%of the particles in the droplet are captured within three shots together with shaking pro-cesses.With this system,suspension droplets are generated and droplets containing only oneyeast cll can be recognized,then each single cell is cultured in the array of the chip.Byobservingtheir growth in cell numbers and the green fluorescence protein(GFP)production via fluorescence imaging,the single cell with the highest production can be identified.The results haveproved the heterogeneity of yeast cells,and showed that the combined system can be applied forrapid single-cell sorting,cultivation,and analysis.展开更多
基于图像处理的景深延拓算法(Extend Depth of Field,EDF)指提取聚焦于样本不同区域的图像中的清晰部分,将其融合到一张图像中,使融合图像中样本的各个区域都清晰。文中提出了一种针对视频流的景深延拓算法,首先通过差值图像筛选出被认...基于图像处理的景深延拓算法(Extend Depth of Field,EDF)指提取聚焦于样本不同区域的图像中的清晰部分,将其融合到一张图像中,使融合图像中样本的各个区域都清晰。文中提出了一种针对视频流的景深延拓算法,首先通过差值图像筛选出被认为是焦面深度发生变化的图像;然后进行配准,减小融合误差;最后使用基于拉普拉斯金字塔的图像融合算法,与前一次的融合图像融合,通过重复这个过程来实现视频流的实时动态景深延拓。以基于空间域的图像融合算法和基于小波变换的图像融合算法为参照,从主观和客观角度比较了视频流场景下的运行效率和融合质量,实验结果表明基于拉普拉斯金字塔的图像融合算法具有较好的运算效率,而且对输入图像中存在离焦模糊的情况具有鲁棒性。展开更多
基金This work was supported by the National Natural Science Foundation of China(Grant Nos.61822505,11774101,61627827,and 81630046)the Science and Technology Planning Project of Guangdong Province,China(No.2015B020233016)the Science and Technology Program of Guangzhou(No.2019050001).
文摘Faster and better wound healing is a critical medical issue.Because the repair process of wounds is closely related to revascularization,accurate early assessment and postoperative monitoring are very important for establishing an optimal treatment plan.Herein,we present an extended depth-of-field photoacoustic microscopy system(E-DOF-PAM)that can achieve a constant spatial resolution and relatively uniform excitation efficiency over a long axial range.The superior performance of the system was verified by phantom and in vivo experiments.Furthermore,the system was applied to the imaging of normal and trauma sites of volunteers,and the experimental results accurately revealed the morphological differences between the normal and traumatized skin of the epidermis and dermis.These results demonstrated that the E-DOF-PAM is a powerful tool for observing and understanding the pathophysiology of cutaneous wound healing.
基金supported by the National Key R&D Program of China(2021YFF0502900)the National Natural Science Foundation of China(62175034,62175036)+7 种基金the Anhui Province KeyR&D Project(202003a07020020)the ShanghaiNatural Science Foundation(grant No.20ZR1405100)the Science and Technology Research Program ofShanghai(grant No.19DZ2282100)the Shanghaikey discipline construction plan(2020-2022)(grantNo.GWV-10.1-XK01)the Shanghai EngineeringTechnology Research Center of Hair Medicine(19DZ2250500)the Medical Engineering Fund of Fudan University(yg2021-022)the Pioneering Project of Academy for Engineering and Technology,the Fudan University(gy2018-001,gy2018-002)the Yantai Returned Scholars'Pioneering Park.
文摘Microfuidic systems have been widely utilized in high-throughput biology analysis,but thedificulties in iquid manipulation and cell cultivation limit its application.This work has developed a new digital microfluidic(DMF)system for on-demand droplet control.By adopting anextending-depth-of-field(EDoF)phase modulator to the optical system,the entire depth of themicrofluidic channel can be covered in one image without any refocusing process,ensuring that 95%of the particles in the droplet are captured within three shots together with shaking pro-cesses.With this system,suspension droplets are generated and droplets containing only oneyeast cll can be recognized,then each single cell is cultured in the array of the chip.Byobservingtheir growth in cell numbers and the green fluorescence protein(GFP)production via fluorescence imaging,the single cell with the highest production can be identified.The results haveproved the heterogeneity of yeast cells,and showed that the combined system can be applied forrapid single-cell sorting,cultivation,and analysis.
文摘基于图像处理的景深延拓算法(Extend Depth of Field,EDF)指提取聚焦于样本不同区域的图像中的清晰部分,将其融合到一张图像中,使融合图像中样本的各个区域都清晰。文中提出了一种针对视频流的景深延拓算法,首先通过差值图像筛选出被认为是焦面深度发生变化的图像;然后进行配准,减小融合误差;最后使用基于拉普拉斯金字塔的图像融合算法,与前一次的融合图像融合,通过重复这个过程来实现视频流的实时动态景深延拓。以基于空间域的图像融合算法和基于小波变换的图像融合算法为参照,从主观和客观角度比较了视频流场景下的运行效率和融合质量,实验结果表明基于拉普拉斯金字塔的图像融合算法具有较好的运算效率,而且对输入图像中存在离焦模糊的情况具有鲁棒性。
