Guided bone regeneration (GBR) often utilizes a combination of autologous bone grafts, deproteinized bovine bone mineral(DBBM), and collagen membranes. DBBM and collagen membranes pre-coated with bone-conditioned medi...Guided bone regeneration (GBR) often utilizes a combination of autologous bone grafts, deproteinized bovine bone mineral(DBBM), and collagen membranes. DBBM and collagen membranes pre-coated with bone-conditioned medium (BCM) extracted from locally harvested autologous bone chips have shown great regenerative potential in GBR. However, the underlying molecular mechanism remains largely unknown. Here, we investigated the composition of BCM and its activity on the osteogenic potential of mesenchymal stromal cells. We detected a fast and significant (P <0.001) release of transforming growth factor-β1 (TGF-β1) from autologous bone within 10 min versus a delayed bone morphogenetic protein-2 (BMP-2) release from 40 min onwards. BCMs harvested within short time periods (10, 20, or 40 min), corresponding to the time of a typical surgical procedure, significantly increased the proliferative activity and collagen matrix production of BCM-treated cells. Long-term (1, 3, or 6 days)-extracted BCMs promoted the later stages of osteoblast differentiation and maturation. Short-term-extracted BCMs, in which TGF-β1 but no BMP-2was detected, reduced the expression of the late differentiation marker osteocalcin. However, when both growth factors were present simultaneously in the BCM, no inhibitory effects on osteoblast differentiation were observed, suggesting a synergistic TGF-β1/BMP-2 activity. Consequently, in cells that were co-stimulated with recombinant TGF-β1 and BMP-2, we showed a significant stimulatory and dose-dependent effect of TGF-β1 on BMP-2-induced osteoblast differentiation due to prolonged BMP signaling and reduced expression of the BMP-2 antagonist noggin. Altogether, our data provide new insights into the molecular mechanisms underlying the favorable outcome from GBR procedures using BCM, derived from autologous bone grafts.展开更多
WANGFUJlNG Street is one of China＇s most renowned shopping streets. The area＇s commercial history can date back many centuries. Today, the street located in the eastern-central side of the city is always choked with...WANGFUJlNG Street is one of China＇s most renowned shopping streets. The area＇s commercial history can date back many centuries. Today, the street located in the eastern-central side of the city is always choked with happy＇shoppers and tourists from home and abroad. One August morning, people found that the street. congested as usual, had somehow changed - in the middle stood a row of panels which displayed pictures representing another country. ＂The photo exhibition, titled ＇South Africa in Beijing,＇ seeks to showcase the very rich and diverse South African culture and heritage to the broad Chinese public,＂ said Dr. Bheki Langa, South Africa＇s Ambassador to China.展开更多
Chinese entrepreneurs can be found scattered across the globe, all with an innate sense of business that has made them a potent force ininternational trade . To illustrate the importance of entrepreneurs, at the 100th...Chinese entrepreneurs can be found scattered across the globe, all with an innate sense of business that has made them a potent force ininternational trade . To illustrate the importance of entrepreneurs, at the 100th Chinese Export Commodities Fair (CECF) held in Guangzhou in October, Premier Wen Jiabao met with Zheng Mingru, Permanent Honorary President of the Wor ld Chinese Entrepr eneurs General Association (WCEGA), and awar ded him a medal for his sterling wor k in connecting Chinese business people wo...展开更多
THE quadrangle is recognized as a masterpiece of traditional Chinese dwellings.From the bricks to the tiles,and beams to the pillars,this type of residence offers a template of Chinese aesthetics.Exhibiting the profou...THE quadrangle is recognized as a masterpiece of traditional Chinese dwellings.From the bricks to the tiles,and beams to the pillars,this type of residence offers a template of Chinese aesthetics.Exhibiting the profound and longstanding Chinese culture,the courtyards are valuable architectural and cultural heritages for China and even the rest of the world.展开更多
目的观察益气化瘀解毒法对慢性萎缩性胃炎(CAG)伴异型增生(Dys)模型大鼠EGFR/MAPK信号通路的影响。方法 60只SPF级健康雄性Wistar大鼠随机分为空白组10只,造模组50只。空白组给予SPF级动物标准饮食喂养,持续至实验结束。造模组采用以120...目的观察益气化瘀解毒法对慢性萎缩性胃炎(CAG)伴异型增生(Dys)模型大鼠EGFR/MAPK信号通路的影响。方法 60只SPF级健康雄性Wistar大鼠随机分为空白组10只,造模组50只。空白组给予SPF级动物标准饮食喂养,持续至实验结束。造模组采用以120 mg/L的N-甲基-N'-硝基-N-亚硝基胍(MNNG)溶液灌胃,每只5 m L/kg,1次/d为主,配合自由饮用0.1%氨水溶液及进食含0.03%雷尼替丁的颗粒状饲料,3种因素联合建立CAG伴Dys大鼠模型。28周末造模成功后将造模组剩余的30只大鼠随机分为模型组、维酶素组、益气化瘀解毒法组(中药组),每组各10只,均给予SPF级动物标准饮食喂养,并且每天灌胃1次,维酶素组予维酶素悬浊液2 m L/kg(即维酶素0.3 g/kg),中药组予益气化瘀解毒中药3 m L/kg(含生药9 g/kg),模型组予生理盐水3m L/kg。12周后即实验第40周末处死全部大鼠。选用Western blot法对各组大鼠胃窦部黏膜组织中EGFR、ERK1/2、p-ERK1/2蛋白表达进行检测。结果模型组EGFR蛋白表达最高,其次是维酶素组、中药组、空白组;其中,模型组与空白组和中药组比较均有显著性差异(均P<0.01),中药组与维酶素组比较有显著性差异(P<0.05)、与空白组比较无显著性差异(P>0.05)。模型组ERK1/2蛋白表达最高,其次是维酶素组、中药组、空白组;其中,模型组、维酶素组与空白组和中药组比较均有显著性差异(均P<0.01),中药组与空白组比较也有显著性差异(P<0.01)。模型组p-ERK1/2蛋白表达最高,其次是维酶素组、中药组、空白组;其中,模型组与空白组和中药组比较均有显著性差异(均P<0.01),中药组与维酶素组有显著性差异(P<0.01)、与空白组比较无显著性差异。结论益气化瘀解毒法可显著抑制EGFR的高表达及EGFR/MAPK细胞信号转导通路的异常激活,这可能是其治疗CAG伴Dys、逆转胃癌前病变(PLGC)、防治胃癌的有效作用机制之一。展开更多
基金supported by a grant from the ITI International Team for Implantology Foundation 1235_2017 to M.B.A
文摘Guided bone regeneration (GBR) often utilizes a combination of autologous bone grafts, deproteinized bovine bone mineral(DBBM), and collagen membranes. DBBM and collagen membranes pre-coated with bone-conditioned medium (BCM) extracted from locally harvested autologous bone chips have shown great regenerative potential in GBR. However, the underlying molecular mechanism remains largely unknown. Here, we investigated the composition of BCM and its activity on the osteogenic potential of mesenchymal stromal cells. We detected a fast and significant (P <0.001) release of transforming growth factor-β1 (TGF-β1) from autologous bone within 10 min versus a delayed bone morphogenetic protein-2 (BMP-2) release from 40 min onwards. BCMs harvested within short time periods (10, 20, or 40 min), corresponding to the time of a typical surgical procedure, significantly increased the proliferative activity and collagen matrix production of BCM-treated cells. Long-term (1, 3, or 6 days)-extracted BCMs promoted the later stages of osteoblast differentiation and maturation. Short-term-extracted BCMs, in which TGF-β1 but no BMP-2was detected, reduced the expression of the late differentiation marker osteocalcin. However, when both growth factors were present simultaneously in the BCM, no inhibitory effects on osteoblast differentiation were observed, suggesting a synergistic TGF-β1/BMP-2 activity. Consequently, in cells that were co-stimulated with recombinant TGF-β1 and BMP-2, we showed a significant stimulatory and dose-dependent effect of TGF-β1 on BMP-2-induced osteoblast differentiation due to prolonged BMP signaling and reduced expression of the BMP-2 antagonist noggin. Altogether, our data provide new insights into the molecular mechanisms underlying the favorable outcome from GBR procedures using BCM, derived from autologous bone grafts.
文摘WANGFUJlNG Street is one of China＇s most renowned shopping streets. The area＇s commercial history can date back many centuries. Today, the street located in the eastern-central side of the city is always choked with happy＇shoppers and tourists from home and abroad. One August morning, people found that the street. congested as usual, had somehow changed - in the middle stood a row of panels which displayed pictures representing another country. ＂The photo exhibition, titled ＇South Africa in Beijing,＇ seeks to showcase the very rich and diverse South African culture and heritage to the broad Chinese public,＂ said Dr. Bheki Langa, South Africa＇s Ambassador to China.
文摘Chinese entrepreneurs can be found scattered across the globe, all with an innate sense of business that has made them a potent force ininternational trade . To illustrate the importance of entrepreneurs, at the 100th Chinese Export Commodities Fair (CECF) held in Guangzhou in October, Premier Wen Jiabao met with Zheng Mingru, Permanent Honorary President of the Wor ld Chinese Entrepr eneurs General Association (WCEGA), and awar ded him a medal for his sterling wor k in connecting Chinese business people wo...
文摘THE quadrangle is recognized as a masterpiece of traditional Chinese dwellings.From the bricks to the tiles,and beams to the pillars,this type of residence offers a template of Chinese aesthetics.Exhibiting the profound and longstanding Chinese culture,the courtyards are valuable architectural and cultural heritages for China and even the rest of the world.
文摘目的观察益气化瘀解毒法对慢性萎缩性胃炎(CAG)伴异型增生(Dys)模型大鼠EGFR/MAPK信号通路的影响。方法 60只SPF级健康雄性Wistar大鼠随机分为空白组10只,造模组50只。空白组给予SPF级动物标准饮食喂养,持续至实验结束。造模组采用以120 mg/L的N-甲基-N'-硝基-N-亚硝基胍(MNNG)溶液灌胃,每只5 m L/kg,1次/d为主,配合自由饮用0.1%氨水溶液及进食含0.03%雷尼替丁的颗粒状饲料,3种因素联合建立CAG伴Dys大鼠模型。28周末造模成功后将造模组剩余的30只大鼠随机分为模型组、维酶素组、益气化瘀解毒法组(中药组),每组各10只,均给予SPF级动物标准饮食喂养,并且每天灌胃1次,维酶素组予维酶素悬浊液2 m L/kg(即维酶素0.3 g/kg),中药组予益气化瘀解毒中药3 m L/kg(含生药9 g/kg),模型组予生理盐水3m L/kg。12周后即实验第40周末处死全部大鼠。选用Western blot法对各组大鼠胃窦部黏膜组织中EGFR、ERK1/2、p-ERK1/2蛋白表达进行检测。结果模型组EGFR蛋白表达最高,其次是维酶素组、中药组、空白组;其中,模型组与空白组和中药组比较均有显著性差异(均P<0.01),中药组与维酶素组比较有显著性差异(P<0.05)、与空白组比较无显著性差异(P>0.05)。模型组ERK1/2蛋白表达最高,其次是维酶素组、中药组、空白组;其中,模型组、维酶素组与空白组和中药组比较均有显著性差异(均P<0.01),中药组与空白组比较也有显著性差异(P<0.01)。模型组p-ERK1/2蛋白表达最高,其次是维酶素组、中药组、空白组;其中,模型组与空白组和中药组比较均有显著性差异(均P<0.01),中药组与维酶素组有显著性差异(P<0.01)、与空白组比较无显著性差异。结论益气化瘀解毒法可显著抑制EGFR的高表达及EGFR/MAPK细胞信号转导通路的异常激活,这可能是其治疗CAG伴Dys、逆转胃癌前病变(PLGC)、防治胃癌的有效作用机制之一。
