a-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors are considered to play a crucial role in synaptic plasticity in the developing visual cortex. In this study, we established a rat model of binocular form ...a-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors are considered to play a crucial role in synaptic plasticity in the developing visual cortex. In this study, we established a rat model of binocular form deprivation by suturing the rat binocular eyelids before eye-opening at postnatal day 14. During development, the decay time of excitatory postsynaptic currents mediated by a-amino-3- hydroxy-5-methyl-4-isoxazolepropionic acid receptors of normal rats became longer after eye- opening; however, the decay time did not change significantly in binocular form deprivation rats. The peak value in the normal group became gradually larger with age, but there was no significant change in the binocular form deprivation group. These findings indicate that binocular form deprivation influences the properties of excitatory postsynaptic currents mediated by a-amino-3- hydroxy-5-methyl-4-isoxazolepropionic acid receptors in the rat visual cortex around the end of the critical period, indicating that form stimulation is associated with the experience-dependent modification of neuronal synapses in the visual cortex.展开更多
目的:初步探讨β淀粉样蛋白Aβ_(25-35)损伤海马CA1区兴奋性突触的靶点及贝沙罗汀的可能拮抗效应。方法:以出生7~14 d Wistar大鼠海马脑片为研究对象,采用全细胞膜片钳技术,在电压钳模式下记录大鼠海马脑片CA1区锥体细胞自发兴奋性突触...目的:初步探讨β淀粉样蛋白Aβ_(25-35)损伤海马CA1区兴奋性突触的靶点及贝沙罗汀的可能拮抗效应。方法:以出生7~14 d Wistar大鼠海马脑片为研究对象,采用全细胞膜片钳技术,在电压钳模式下记录大鼠海马脑片CA1区锥体细胞自发兴奋性突触后电流(s EPSCs)和微小兴奋性突触后电流(mEPSCs),分析不同组神经元s EPSCs和mEPSCs幅度及频率的差异。结果:与对照组相比,经Aβ_(25-35)(1μmol/L)处理后,海马神经元sEPSCs与mEPSCs平均幅度和平均频率都显著降低(均P<0.05)。向Aβ_(25-35)处理过的海马脑片中加入贝沙罗汀(5μmol/L)后,sEPSCs与mEPSCs平均幅度和平均频率较Aβ_(25-35)组都显著提高(均P<0.05)。贝沙罗汀处理组sEPSCs与mEPSCs平均频率和平均幅度与对照组水平无显著性差异(均P>0.05)。结论:Aβ_(25-35)可作用于CA1区,导致海马锥体神经元兴奋性突触后电位降低,突触功能损伤,贝沙罗汀通过作用于突触前和突触后位点拮抗Aβ_(25-35)的损伤效应。展开更多
基金the National Natural Science Foundation of China, No.30772350
文摘a-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors are considered to play a crucial role in synaptic plasticity in the developing visual cortex. In this study, we established a rat model of binocular form deprivation by suturing the rat binocular eyelids before eye-opening at postnatal day 14. During development, the decay time of excitatory postsynaptic currents mediated by a-amino-3- hydroxy-5-methyl-4-isoxazolepropionic acid receptors of normal rats became longer after eye- opening; however, the decay time did not change significantly in binocular form deprivation rats. The peak value in the normal group became gradually larger with age, but there was no significant change in the binocular form deprivation group. These findings indicate that binocular form deprivation influences the properties of excitatory postsynaptic currents mediated by a-amino-3- hydroxy-5-methyl-4-isoxazolepropionic acid receptors in the rat visual cortex around the end of the critical period, indicating that form stimulation is associated with the experience-dependent modification of neuronal synapses in the visual cortex.
文摘目的:初步探讨β淀粉样蛋白Aβ_(25-35)损伤海马CA1区兴奋性突触的靶点及贝沙罗汀的可能拮抗效应。方法:以出生7~14 d Wistar大鼠海马脑片为研究对象,采用全细胞膜片钳技术,在电压钳模式下记录大鼠海马脑片CA1区锥体细胞自发兴奋性突触后电流(s EPSCs)和微小兴奋性突触后电流(mEPSCs),分析不同组神经元s EPSCs和mEPSCs幅度及频率的差异。结果:与对照组相比,经Aβ_(25-35)(1μmol/L)处理后,海马神经元sEPSCs与mEPSCs平均幅度和平均频率都显著降低(均P<0.05)。向Aβ_(25-35)处理过的海马脑片中加入贝沙罗汀(5μmol/L)后,sEPSCs与mEPSCs平均幅度和平均频率较Aβ_(25-35)组都显著提高(均P<0.05)。贝沙罗汀处理组sEPSCs与mEPSCs平均频率和平均幅度与对照组水平无显著性差异(均P>0.05)。结论:Aβ_(25-35)可作用于CA1区,导致海马锥体神经元兴奋性突触后电位降低,突触功能损伤,贝沙罗汀通过作用于突触前和突触后位点拮抗Aβ_(25-35)的损伤效应。
